Screening of bacterial endophytes as potential biocontrol agents against soybean diseases

Autores
de Almeida Lopes, Karla Bianca; Carpentieri Pipolo, Valéria; Fira, Djordje; Balatti, Pedro Alberto; López, Silvina Marianela Yanil; Oro, Thiago Henrique; Stefani Pagliosa, Eduardo; Degrassi, Giuliano
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: This research was aimed at identifying and characterizing endophytic micro-organisms associated with soybean that have antimicrobial activity towards soybean pathogens. Methods and Results: Soybean plants were collected from field trials in four locations of southern Brazil that were cultivated with conventional (C) and transgenic glyphosate-resistant (GR) soybeans. Endophytic bacteria isolated from roots, stems and leaves of soybeans were evaluated for their capacity to inhibit fungal and bacterial plant pathogens and 13 micro-organisms were identified with antagonistic activity. Approximately 230 bacteria were isolated and identified based on the 16S rRNA and rpoN gene sequences. Bacteria isolated from conventional and transgenic soybeans were significantly different not only in population diversity but also in their antagonistic capacity. Thirteen isolates showed in vitro antagonism against Sclerotinia sclerotiorum, Phomopsis sojae and Rhizoctonia solani. Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling bacterial and fungal pathogens in vitro. Extracts and precipitates from culture supernatants of isolates showed different patterns of inhibitory activity on growth of fungal and bacterial pathogens. Conclusions: Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling fungal pathogens in vitro, and the activity is mainly due to peptides. However, most of the studied bacteria showed the presence of antimicrobial compounds in the culture supernatant, either peptides, bacteriocins or secondary metabolites. Significance and Impact of the Study: These results could be significant to develop tools for the biological control of soybean diseases. The work brought to the identification of micro-organisms such as Bacillus sp. and Burkholderia sp. that have the potential to protect crops in order to enhance a sustainable management system of crops. Furthermore, the study provides the first evidences of the influence of management as well as the genetics of glyphosate-resistant soybean on the diversity of bacterial endophytes of soybean phytobiome.
Fil: de Almeida Lopes, Karla Bianca. Universidade Estadual de Londrina; Brasil
Fil: Carpentieri Pipolo, Valéria. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Fira, Djordje. University of Belgrade; Serbia
Fil: Balatti, Pedro Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: López, Silvina Marianela Yanil. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Oro, Thiago Henrique. Universidade Estadual de Londrina; Brasil
Fil: Stefani Pagliosa, Eduardo. Universidade Estadual de Londrina; Brasil
Fil: Degrassi, Giuliano. International Centre for Genetic Engineering and Biotechnology; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ANTAGONISM
BIOLOGICAL CONTROL
CROP PROTECTION
ENDOPHYTES
GLYPHOSATE-RESISTANT SOYBEAN
PLANT–BACTERIA ASSOCIATION
SOYBEAN PATHOGENS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85405
Acceder
id CONICETDig_45aa87e07b701b28ee847292595ad446
oai_identifier_str oai:ri.conicet.gov.ar:11336/85405
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening of bacterial endophytes as potential biocontrol agents against soybean diseasesde Almeida Lopes, Karla BiancaCarpentieri Pipolo, ValériaFira, DjordjeBalatti, Pedro AlbertoLópez, Silvina Marianela YanilOro, Thiago HenriqueStefani Pagliosa, EduardoDegrassi, GiulianoANTAGONISMBIOLOGICAL CONTROLCROP PROTECTIONENDOPHYTESGLYPHOSATE-RESISTANT SOYBEANPLANT–BACTERIA ASSOCIATIONSOYBEAN PATHOGENShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Aims: This research was aimed at identifying and characterizing endophytic micro-organisms associated with soybean that have antimicrobial activity towards soybean pathogens. Methods and Results: Soybean plants were collected from field trials in four locations of southern Brazil that were cultivated with conventional (C) and transgenic glyphosate-resistant (GR) soybeans. Endophytic bacteria isolated from roots, stems and leaves of soybeans were evaluated for their capacity to inhibit fungal and bacterial plant pathogens and 13 micro-organisms were identified with antagonistic activity. Approximately 230 bacteria were isolated and identified based on the 16S rRNA and rpoN gene sequences. Bacteria isolated from conventional and transgenic soybeans were significantly different not only in population diversity but also in their antagonistic capacity. Thirteen isolates showed in vitro antagonism against Sclerotinia sclerotiorum, Phomopsis sojae and Rhizoctonia solani. Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling bacterial and fungal pathogens in vitro. Extracts and precipitates from culture supernatants of isolates showed different patterns of inhibitory activity on growth of fungal and bacterial pathogens. Conclusions: Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling fungal pathogens in vitro, and the activity is mainly due to peptides. However, most of the studied bacteria showed the presence of antimicrobial compounds in the culture supernatant, either peptides, bacteriocins or secondary metabolites. Significance and Impact of the Study: These results could be significant to develop tools for the biological control of soybean diseases. The work brought to the identification of micro-organisms such as Bacillus sp. and Burkholderia sp. that have the potential to protect crops in order to enhance a sustainable management system of crops. Furthermore, the study provides the first evidences of the influence of management as well as the genetics of glyphosate-resistant soybean on the diversity of bacterial endophytes of soybean phytobiome.Fil: de Almeida Lopes, Karla Bianca. Universidade Estadual de Londrina; BrasilFil: Carpentieri Pipolo, Valéria. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Fira, Djordje. University of Belgrade; SerbiaFil: Balatti, Pedro Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaFil: López, Silvina Marianela Yanil. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Oro, Thiago Henrique. Universidade Estadual de Londrina; BrasilFil: Stefani Pagliosa, Eduardo. Universidade Estadual de Londrina; BrasilFil: Degrassi, Giuliano. International Centre for Genetic Engineering and Biotechnology; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85405de Almeida Lopes, Karla Bianca; Carpentieri Pipolo, Valéria; Fira, Djordje; Balatti, Pedro Alberto; López, Silvina Marianela Yanil; et al.; Screening of bacterial endophytes as potential biocontrol agents against soybean diseases; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 125; 5; 11-2018; 1466-14811364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14041info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.14041info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:00:22Zoai:ri.conicet.gov.ar:11336/85405instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:00:22.865CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
title Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
spellingShingle Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
de Almeida Lopes, Karla Bianca
ANTAGONISM
BIOLOGICAL CONTROL
CROP PROTECTION
ENDOPHYTES
GLYPHOSATE-RESISTANT SOYBEAN
PLANT–BACTERIA ASSOCIATION
SOYBEAN PATHOGENS
title_short Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
title_full Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
title_fullStr Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
title_full_unstemmed Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
title_sort Screening of bacterial endophytes as potential biocontrol agents against soybean diseases
dc.creator.none.fl_str_mv de Almeida Lopes, Karla Bianca
Carpentieri Pipolo, Valéria
Fira, Djordje
Balatti, Pedro Alberto
López, Silvina Marianela Yanil
Oro, Thiago Henrique
Stefani Pagliosa, Eduardo
Degrassi, Giuliano
author de Almeida Lopes, Karla Bianca
author_facet de Almeida Lopes, Karla Bianca
Carpentieri Pipolo, Valéria
Fira, Djordje
Balatti, Pedro Alberto
López, Silvina Marianela Yanil
Oro, Thiago Henrique
Stefani Pagliosa, Eduardo
Degrassi, Giuliano
author_role author
author2 Carpentieri Pipolo, Valéria
Fira, Djordje
Balatti, Pedro Alberto
López, Silvina Marianela Yanil
Oro, Thiago Henrique
Stefani Pagliosa, Eduardo
Degrassi, Giuliano
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTAGONISM
BIOLOGICAL CONTROL
CROP PROTECTION
ENDOPHYTES
GLYPHOSATE-RESISTANT SOYBEAN
PLANT–BACTERIA ASSOCIATION
SOYBEAN PATHOGENS
topic ANTAGONISM
BIOLOGICAL CONTROL
CROP PROTECTION
ENDOPHYTES
GLYPHOSATE-RESISTANT SOYBEAN
PLANT–BACTERIA ASSOCIATION
SOYBEAN PATHOGENS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Aims: This research was aimed at identifying and characterizing endophytic micro-organisms associated with soybean that have antimicrobial activity towards soybean pathogens. Methods and Results: Soybean plants were collected from field trials in four locations of southern Brazil that were cultivated with conventional (C) and transgenic glyphosate-resistant (GR) soybeans. Endophytic bacteria isolated from roots, stems and leaves of soybeans were evaluated for their capacity to inhibit fungal and bacterial plant pathogens and 13 micro-organisms were identified with antagonistic activity. Approximately 230 bacteria were isolated and identified based on the 16S rRNA and rpoN gene sequences. Bacteria isolated from conventional and transgenic soybeans were significantly different not only in population diversity but also in their antagonistic capacity. Thirteen isolates showed in vitro antagonism against Sclerotinia sclerotiorum, Phomopsis sojae and Rhizoctonia solani. Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling bacterial and fungal pathogens in vitro. Extracts and precipitates from culture supernatants of isolates showed different patterns of inhibitory activity on growth of fungal and bacterial pathogens. Conclusions: Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling fungal pathogens in vitro, and the activity is mainly due to peptides. However, most of the studied bacteria showed the presence of antimicrobial compounds in the culture supernatant, either peptides, bacteriocins or secondary metabolites. Significance and Impact of the Study: These results could be significant to develop tools for the biological control of soybean diseases. The work brought to the identification of micro-organisms such as Bacillus sp. and Burkholderia sp. that have the potential to protect crops in order to enhance a sustainable management system of crops. Furthermore, the study provides the first evidences of the influence of management as well as the genetics of glyphosate-resistant soybean on the diversity of bacterial endophytes of soybean phytobiome.
Fil: de Almeida Lopes, Karla Bianca. Universidade Estadual de Londrina; Brasil
Fil: Carpentieri Pipolo, Valéria. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Fira, Djordje. University of Belgrade; Serbia
Fil: Balatti, Pedro Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Fil: López, Silvina Marianela Yanil. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Oro, Thiago Henrique. Universidade Estadual de Londrina; Brasil
Fil: Stefani Pagliosa, Eduardo. Universidade Estadual de Londrina; Brasil
Fil: Degrassi, Giuliano. International Centre for Genetic Engineering and Biotechnology; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aims: This research was aimed at identifying and characterizing endophytic micro-organisms associated with soybean that have antimicrobial activity towards soybean pathogens. Methods and Results: Soybean plants were collected from field trials in four locations of southern Brazil that were cultivated with conventional (C) and transgenic glyphosate-resistant (GR) soybeans. Endophytic bacteria isolated from roots, stems and leaves of soybeans were evaluated for their capacity to inhibit fungal and bacterial plant pathogens and 13 micro-organisms were identified with antagonistic activity. Approximately 230 bacteria were isolated and identified based on the 16S rRNA and rpoN gene sequences. Bacteria isolated from conventional and transgenic soybeans were significantly different not only in population diversity but also in their antagonistic capacity. Thirteen isolates showed in vitro antagonism against Sclerotinia sclerotiorum, Phomopsis sojae and Rhizoctonia solani. Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling bacterial and fungal pathogens in vitro. Extracts and precipitates from culture supernatants of isolates showed different patterns of inhibitory activity on growth of fungal and bacterial pathogens. Conclusions: Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling fungal pathogens in vitro, and the activity is mainly due to peptides. However, most of the studied bacteria showed the presence of antimicrobial compounds in the culture supernatant, either peptides, bacteriocins or secondary metabolites. Significance and Impact of the Study: These results could be significant to develop tools for the biological control of soybean diseases. The work brought to the identification of micro-organisms such as Bacillus sp. and Burkholderia sp. that have the potential to protect crops in order to enhance a sustainable management system of crops. Furthermore, the study provides the first evidences of the influence of management as well as the genetics of glyphosate-resistant soybean on the diversity of bacterial endophytes of soybean phytobiome.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85405
de Almeida Lopes, Karla Bianca; Carpentieri Pipolo, Valéria; Fira, Djordje; Balatti, Pedro Alberto; López, Silvina Marianela Yanil; et al.; Screening of bacterial endophytes as potential biocontrol agents against soybean diseases; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 125; 5; 11-2018; 1466-1481
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85405
identifier_str_mv de Almeida Lopes, Karla Bianca; Carpentieri Pipolo, Valéria; Fira, Djordje; Balatti, Pedro Alberto; López, Silvina Marianela Yanil; et al.; Screening of bacterial endophytes as potential biocontrol agents against soybean diseases; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 125; 5; 11-2018; 1466-1481
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14041
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.14041
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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