Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal

Autores
Di Bella, Daniela Jesica; Carcagno, Abel Luis; Bartolomeu, M. Lucía; Pardi, Belén; Löhr, Heiko; Siegel, Nicole; Hammerschmidt, Matthias; Marin Burgin, Antonia; Lanuza, Guillermo Marcos
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Generation of neuronal types at the right time, location, and number is essential for building a functional nervous system. Significant progress has been reached in understanding the mechanisms that govern neuronal diversity. Cerebrospinal fluid-contacting neurons (CSF-cNs), an intriguing spinal cord central canal population, are produced during advanced developmental stages, simultaneous with glial and ependymal cells. It is unknown how CSF-cNs are specified after the neurogenesis-to-gliogenesis switch. Here, we identify delayed Ascl1 expression in mouse spinal progenitors during the gliogenic phase as key in CSF-cN differentiation. With fate mappings and time-controlled deletions, we demonstrate that CSF-cNs derive from Ascl1-expressing cells and that Ascl1 triggers late neurogenesis in the amniote spinal cord. Ascl1 abrogation transforms prospective CSF-cN progenitors into ependymocytes. These results demonstrate that late spinal progenitors have the potential to produce neurons and that Ascl1 initiates CSF-cN differentiation, controlling the precise neuronal and nonneuronal composition of the spinal central canal.
Fil: Di Bella, Daniela Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Instituto Leloir; Argentina
Fil: Carcagno, Abel Luis. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bartolomeu, M. Lucía. Fundación Instituto Leloir; Argentina
Fil: Pardi, Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Löhr, Heiko. University of Cologne; Alemania
Fil: Siegel, Nicole. Fundación Instituto Leloir; Argentina
Fil: Hammerschmidt, Matthias. University of Cologne; Alemania
Fil: Marin Burgin, Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Lanuza, Guillermo Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Instituto Leloir; Argentina
Materia
ASCL1
CENTRAL CANAL
CSF-CN
CSF-CONTACTING NEURONS
EPENDYMOCYTES
LATE NEUROGENESIS
NEURAL PROGENITOR
NEURON SPECIFICATION
SPINAL CORD
TRANSCRIPTION FACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94162
Acceder
id CONICETDig_45090498edd5606c7da472b6a6b081ad
oai_identifier_str oai:ri.conicet.gov.ar:11336/94162
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central CanalDi Bella, Daniela JesicaCarcagno, Abel LuisBartolomeu, M. LucíaPardi, BelénLöhr, HeikoSiegel, NicoleHammerschmidt, MatthiasMarin Burgin, AntoniaLanuza, Guillermo MarcosASCL1CENTRAL CANALCSF-CNCSF-CONTACTING NEURONSEPENDYMOCYTESLATE NEUROGENESISNEURAL PROGENITORNEURON SPECIFICATIONSPINAL CORDTRANSCRIPTION FACTORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Generation of neuronal types at the right time, location, and number is essential for building a functional nervous system. Significant progress has been reached in understanding the mechanisms that govern neuronal diversity. Cerebrospinal fluid-contacting neurons (CSF-cNs), an intriguing spinal cord central canal population, are produced during advanced developmental stages, simultaneous with glial and ependymal cells. It is unknown how CSF-cNs are specified after the neurogenesis-to-gliogenesis switch. Here, we identify delayed Ascl1 expression in mouse spinal progenitors during the gliogenic phase as key in CSF-cN differentiation. With fate mappings and time-controlled deletions, we demonstrate that CSF-cNs derive from Ascl1-expressing cells and that Ascl1 triggers late neurogenesis in the amniote spinal cord. Ascl1 abrogation transforms prospective CSF-cN progenitors into ependymocytes. These results demonstrate that late spinal progenitors have the potential to produce neurons and that Ascl1 initiates CSF-cN differentiation, controlling the precise neuronal and nonneuronal composition of the spinal central canal.Fil: Di Bella, Daniela Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Instituto Leloir; ArgentinaFil: Carcagno, Abel Luis. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bartolomeu, M. Lucía. Fundación Instituto Leloir; ArgentinaFil: Pardi, Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Löhr, Heiko. University of Cologne; AlemaniaFil: Siegel, Nicole. Fundación Instituto Leloir; ArgentinaFil: Hammerschmidt, Matthias. University of Cologne; AlemaniaFil: Marin Burgin, Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Lanuza, Guillermo Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Instituto Leloir; ArgentinaElsevier2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94162Di Bella, Daniela Jesica; Carcagno, Abel Luis; Bartolomeu, M. Lucía; Pardi, Belén; Löhr, Heiko; et al.; Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal; Elsevier; Cell Reports; 28; 9; 8-2019; 2264-2274.e32211-1247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2019.07.087info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124719310034info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:50Zoai:ri.conicet.gov.ar:11336/94162instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:50.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
title Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
spellingShingle Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
Di Bella, Daniela Jesica
ASCL1
CENTRAL CANAL
CSF-CN
CSF-CONTACTING NEURONS
EPENDYMOCYTES
LATE NEUROGENESIS
NEURAL PROGENITOR
NEURON SPECIFICATION
SPINAL CORD
TRANSCRIPTION FACTOR
title_short Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
title_full Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
title_fullStr Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
title_full_unstemmed Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
title_sort Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal
dc.creator.none.fl_str_mv Di Bella, Daniela Jesica
Carcagno, Abel Luis
Bartolomeu, M. Lucía
Pardi, Belén
Löhr, Heiko
Siegel, Nicole
Hammerschmidt, Matthias
Marin Burgin, Antonia
Lanuza, Guillermo Marcos
author Di Bella, Daniela Jesica
author_facet Di Bella, Daniela Jesica
Carcagno, Abel Luis
Bartolomeu, M. Lucía
Pardi, Belén
Löhr, Heiko
Siegel, Nicole
Hammerschmidt, Matthias
Marin Burgin, Antonia
Lanuza, Guillermo Marcos
author_role author
author2 Carcagno, Abel Luis
Bartolomeu, M. Lucía
Pardi, Belén
Löhr, Heiko
Siegel, Nicole
Hammerschmidt, Matthias
Marin Burgin, Antonia
Lanuza, Guillermo Marcos
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ASCL1
CENTRAL CANAL
CSF-CN
CSF-CONTACTING NEURONS
EPENDYMOCYTES
LATE NEUROGENESIS
NEURAL PROGENITOR
NEURON SPECIFICATION
SPINAL CORD
TRANSCRIPTION FACTOR
topic ASCL1
CENTRAL CANAL
CSF-CN
CSF-CONTACTING NEURONS
EPENDYMOCYTES
LATE NEUROGENESIS
NEURAL PROGENITOR
NEURON SPECIFICATION
SPINAL CORD
TRANSCRIPTION FACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Generation of neuronal types at the right time, location, and number is essential for building a functional nervous system. Significant progress has been reached in understanding the mechanisms that govern neuronal diversity. Cerebrospinal fluid-contacting neurons (CSF-cNs), an intriguing spinal cord central canal population, are produced during advanced developmental stages, simultaneous with glial and ependymal cells. It is unknown how CSF-cNs are specified after the neurogenesis-to-gliogenesis switch. Here, we identify delayed Ascl1 expression in mouse spinal progenitors during the gliogenic phase as key in CSF-cN differentiation. With fate mappings and time-controlled deletions, we demonstrate that CSF-cNs derive from Ascl1-expressing cells and that Ascl1 triggers late neurogenesis in the amniote spinal cord. Ascl1 abrogation transforms prospective CSF-cN progenitors into ependymocytes. These results demonstrate that late spinal progenitors have the potential to produce neurons and that Ascl1 initiates CSF-cN differentiation, controlling the precise neuronal and nonneuronal composition of the spinal central canal.
Fil: Di Bella, Daniela Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Instituto Leloir; Argentina
Fil: Carcagno, Abel Luis. Fundación Instituto Leloir; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bartolomeu, M. Lucía. Fundación Instituto Leloir; Argentina
Fil: Pardi, Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Löhr, Heiko. University of Cologne; Alemania
Fil: Siegel, Nicole. Fundación Instituto Leloir; Argentina
Fil: Hammerschmidt, Matthias. University of Cologne; Alemania
Fil: Marin Burgin, Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Lanuza, Guillermo Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Instituto Leloir; Argentina
description Generation of neuronal types at the right time, location, and number is essential for building a functional nervous system. Significant progress has been reached in understanding the mechanisms that govern neuronal diversity. Cerebrospinal fluid-contacting neurons (CSF-cNs), an intriguing spinal cord central canal population, are produced during advanced developmental stages, simultaneous with glial and ependymal cells. It is unknown how CSF-cNs are specified after the neurogenesis-to-gliogenesis switch. Here, we identify delayed Ascl1 expression in mouse spinal progenitors during the gliogenic phase as key in CSF-cN differentiation. With fate mappings and time-controlled deletions, we demonstrate that CSF-cNs derive from Ascl1-expressing cells and that Ascl1 triggers late neurogenesis in the amniote spinal cord. Ascl1 abrogation transforms prospective CSF-cN progenitors into ependymocytes. These results demonstrate that late spinal progenitors have the potential to produce neurons and that Ascl1 initiates CSF-cN differentiation, controlling the precise neuronal and nonneuronal composition of the spinal central canal.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94162
Di Bella, Daniela Jesica; Carcagno, Abel Luis; Bartolomeu, M. Lucía; Pardi, Belén; Löhr, Heiko; et al.; Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal; Elsevier; Cell Reports; 28; 9; 8-2019; 2264-2274.e3
2211-1247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94162
identifier_str_mv Di Bella, Daniela Jesica; Carcagno, Abel Luis; Bartolomeu, M. Lucía; Pardi, Belén; Löhr, Heiko; et al.; Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal; Elsevier; Cell Reports; 28; 9; 8-2019; 2264-2274.e3
2211-1247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2019.07.087
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2211124719310034
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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