Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil

Autores
Vannucchi, Francesca; Imperato, Valeria; Saran, Anabel; Staykov, Svetoslav; D'Haen, Jan; Sebastiani, Luca; Vangronsveld, Jaco; Thijs, Sofie
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seed endophyte inoculation can enhance the plant tolerance to pollutants, which allows plant cultivation on trace element (TE) polluted soils. Methylobacterium sp. CP3 and Kineococcus en-dophyticus CP19 were tested in vitro for their tolerance to Zn and Cd and their plant growth promotion traits. The in vivo effects of bioaugmentation with individual strains or both strains were tested using two poplar cultivars, Populus deltoides x (P. trichocarpa x P. maximowiczii) ‘Dender’ and ‘Marke’, grown in TE polluted soil for six weeks. Methylobacterium sp. was found to grow on media enriched with 0.4 and 0.8 mM Cd, and both endophytes tolerated 0.6 and 1 mM Zn, due to the presence of genes involved in Zn and Cd tolerance and transport. Methylobacterium sp. showed an extracellular ion sequestration mechanism. Production of indole-3-acetic acid by Methylobacterium sp. and K. en-dophyticus, as well as phosphorus solubilization by Methylobacterium sp. were observed. Bioaugmen-tation with both endophytes increased the shoot length of Populus ‘Marke’ and enhanced the Mg uptake in both cultivars. Inoculation with Methylobacterium sp. reduced the bioaccumulation of Zn in ‘Marke’, conferring it an excluder strategy. Methylobacterium sp. and K. endophyticus seemed to improve the plant nutritional status, which can alleviate abiotic stress.
Fil: Vannucchi, Francesca. Scuola Superiore Sant’Anna; Italia
Fil: Imperato, Valeria. Hasselt University; Bélgica
Fil: Saran, Anabel. Hasselt University; Bélgica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Staykov, Svetoslav. Hasselt University; Bélgica
Fil: D'Haen, Jan. Hasselt University; Bélgica
Fil: Sebastiani, Luca. Scuola Superiore Sant’Anna; Italia
Fil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie-Skłodowska University; Polonia
Fil: Thijs, Sofie. Hasselt University; Bélgica
Materia
CADMIUM
KINEOCOCCUS ENDOPHYTICUS CP19
METHYLOBACTERIUM SP. CP3
PLANT GROWTH PRO-MOTION
POPULUS
ZINC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151678
Acceder
id CONICETDig_44fc0ba34dbbff6f482aafa02558167d
oai_identifier_str oai:ri.conicet.gov.ar:11336/151678
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted SoilVannucchi, FrancescaImperato, ValeriaSaran, AnabelStaykov, SvetoslavD'Haen, JanSebastiani, LucaVangronsveld, JacoThijs, SofieCADMIUMKINEOCOCCUS ENDOPHYTICUS CP19METHYLOBACTERIUM SP. CP3PLANT GROWTH PRO-MOTIONPOPULUSZINChttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Seed endophyte inoculation can enhance the plant tolerance to pollutants, which allows plant cultivation on trace element (TE) polluted soils. Methylobacterium sp. CP3 and Kineococcus en-dophyticus CP19 were tested in vitro for their tolerance to Zn and Cd and their plant growth promotion traits. The in vivo effects of bioaugmentation with individual strains or both strains were tested using two poplar cultivars, Populus deltoides x (P. trichocarpa x P. maximowiczii) ‘Dender’ and ‘Marke’, grown in TE polluted soil for six weeks. Methylobacterium sp. was found to grow on media enriched with 0.4 and 0.8 mM Cd, and both endophytes tolerated 0.6 and 1 mM Zn, due to the presence of genes involved in Zn and Cd tolerance and transport. Methylobacterium sp. showed an extracellular ion sequestration mechanism. Production of indole-3-acetic acid by Methylobacterium sp. and K. en-dophyticus, as well as phosphorus solubilization by Methylobacterium sp. were observed. Bioaugmen-tation with both endophytes increased the shoot length of Populus ‘Marke’ and enhanced the Mg uptake in both cultivars. Inoculation with Methylobacterium sp. reduced the bioaccumulation of Zn in ‘Marke’, conferring it an excluder strategy. Methylobacterium sp. and K. endophyticus seemed to improve the plant nutritional status, which can alleviate abiotic stress.Fil: Vannucchi, Francesca. Scuola Superiore Sant’Anna; ItaliaFil: Imperato, Valeria. Hasselt University; BélgicaFil: Saran, Anabel. Hasselt University; Bélgica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; ArgentinaFil: Staykov, Svetoslav. Hasselt University; BélgicaFil: D'Haen, Jan. Hasselt University; BélgicaFil: Sebastiani, Luca. Scuola Superiore Sant’Anna; ItaliaFil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie-Skłodowska University; PoloniaFil: Thijs, Sofie. Hasselt University; BélgicaMDPI2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151678Vannucchi, Francesca; Imperato, Valeria; Saran, Anabel; Staykov, Svetoslav; D'Haen, Jan; et al.; Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil; MDPI; Agronomy; 11; 10; 10-2021; 1-172073-4395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/11/10/1987info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy11101987info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-04T10:52:18Zoai:ri.conicet.gov.ar:11336/151678instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-04 10:52:18.754CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
title Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
spellingShingle Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
Vannucchi, Francesca
CADMIUM
KINEOCOCCUS ENDOPHYTICUS CP19
METHYLOBACTERIUM SP. CP3
PLANT GROWTH PRO-MOTION
POPULUS
ZINC
title_short Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
title_full Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
title_fullStr Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
title_full_unstemmed Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
title_sort Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil
dc.creator.none.fl_str_mv Vannucchi, Francesca
Imperato, Valeria
Saran, Anabel
Staykov, Svetoslav
D'Haen, Jan
Sebastiani, Luca
Vangronsveld, Jaco
Thijs, Sofie
author Vannucchi, Francesca
author_facet Vannucchi, Francesca
Imperato, Valeria
Saran, Anabel
Staykov, Svetoslav
D'Haen, Jan
Sebastiani, Luca
Vangronsveld, Jaco
Thijs, Sofie
author_role author
author2 Imperato, Valeria
Saran, Anabel
Staykov, Svetoslav
D'Haen, Jan
Sebastiani, Luca
Vangronsveld, Jaco
Thijs, Sofie
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CADMIUM
KINEOCOCCUS ENDOPHYTICUS CP19
METHYLOBACTERIUM SP. CP3
PLANT GROWTH PRO-MOTION
POPULUS
ZINC
topic CADMIUM
KINEOCOCCUS ENDOPHYTICUS CP19
METHYLOBACTERIUM SP. CP3
PLANT GROWTH PRO-MOTION
POPULUS
ZINC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Seed endophyte inoculation can enhance the plant tolerance to pollutants, which allows plant cultivation on trace element (TE) polluted soils. Methylobacterium sp. CP3 and Kineococcus en-dophyticus CP19 were tested in vitro for their tolerance to Zn and Cd and their plant growth promotion traits. The in vivo effects of bioaugmentation with individual strains or both strains were tested using two poplar cultivars, Populus deltoides x (P. trichocarpa x P. maximowiczii) ‘Dender’ and ‘Marke’, grown in TE polluted soil for six weeks. Methylobacterium sp. was found to grow on media enriched with 0.4 and 0.8 mM Cd, and both endophytes tolerated 0.6 and 1 mM Zn, due to the presence of genes involved in Zn and Cd tolerance and transport. Methylobacterium sp. showed an extracellular ion sequestration mechanism. Production of indole-3-acetic acid by Methylobacterium sp. and K. en-dophyticus, as well as phosphorus solubilization by Methylobacterium sp. were observed. Bioaugmen-tation with both endophytes increased the shoot length of Populus ‘Marke’ and enhanced the Mg uptake in both cultivars. Inoculation with Methylobacterium sp. reduced the bioaccumulation of Zn in ‘Marke’, conferring it an excluder strategy. Methylobacterium sp. and K. endophyticus seemed to improve the plant nutritional status, which can alleviate abiotic stress.
Fil: Vannucchi, Francesca. Scuola Superiore Sant’Anna; Italia
Fil: Imperato, Valeria. Hasselt University; Bélgica
Fil: Saran, Anabel. Hasselt University; Bélgica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Staykov, Svetoslav. Hasselt University; Bélgica
Fil: D'Haen, Jan. Hasselt University; Bélgica
Fil: Sebastiani, Luca. Scuola Superiore Sant’Anna; Italia
Fil: Vangronsveld, Jaco. Hasselt University; Bélgica. Maria Curie-Skłodowska University; Polonia
Fil: Thijs, Sofie. Hasselt University; Bélgica
description Seed endophyte inoculation can enhance the plant tolerance to pollutants, which allows plant cultivation on trace element (TE) polluted soils. Methylobacterium sp. CP3 and Kineococcus en-dophyticus CP19 were tested in vitro for their tolerance to Zn and Cd and their plant growth promotion traits. The in vivo effects of bioaugmentation with individual strains or both strains were tested using two poplar cultivars, Populus deltoides x (P. trichocarpa x P. maximowiczii) ‘Dender’ and ‘Marke’, grown in TE polluted soil for six weeks. Methylobacterium sp. was found to grow on media enriched with 0.4 and 0.8 mM Cd, and both endophytes tolerated 0.6 and 1 mM Zn, due to the presence of genes involved in Zn and Cd tolerance and transport. Methylobacterium sp. showed an extracellular ion sequestration mechanism. Production of indole-3-acetic acid by Methylobacterium sp. and K. en-dophyticus, as well as phosphorus solubilization by Methylobacterium sp. were observed. Bioaugmen-tation with both endophytes increased the shoot length of Populus ‘Marke’ and enhanced the Mg uptake in both cultivars. Inoculation with Methylobacterium sp. reduced the bioaccumulation of Zn in ‘Marke’, conferring it an excluder strategy. Methylobacterium sp. and K. endophyticus seemed to improve the plant nutritional status, which can alleviate abiotic stress.
publishDate 2021
dc.date.none.fl_str_mv 2021-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151678
Vannucchi, Francesca; Imperato, Valeria; Saran, Anabel; Staykov, Svetoslav; D'Haen, Jan; et al.; Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil; MDPI; Agronomy; 11; 10; 10-2021; 1-17
2073-4395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151678
identifier_str_mv Vannucchi, Francesca; Imperato, Valeria; Saran, Anabel; Staykov, Svetoslav; D'Haen, Jan; et al.; Inoculated Seed Endophytes Modify the Poplar Responses to Trace Elements in Polluted Soil; MDPI; Agronomy; 11; 10; 10-2021; 1-17
2073-4395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4395/11/10/1987
info:eu-repo/semantics/altIdentifier/doi/10.3390/agronomy11101987
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.832306

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