Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha

Autores
Gambino, Yésica Paola; Pérez Pérez, Antonio; Dueñas, José L.; Calvo, Juan Carlos; Sánchez Margalet, Víctor; Varone, Cecilia Laura
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology.
Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pérez Pérez, Antonio. Hospital Universitario Virgen Macarena; España
Fil: Dueñas, José L.. Hospital Universitario Virgen Macarena; España
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Sánchez Margalet, Víctor. Hospital Universitario Virgen Macarena; España
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
17β-estradiol
ESTROGEN RECEPTOR
GENE EXPRESSION
LEPTIN
PLACENTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93757
Acceder
id CONICETDig_44f23013c39d99fb25355e91a87b8953
oai_identifier_str oai:ri.conicet.gov.ar:11336/93757
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alphaGambino, Yésica PaolaPérez Pérez, AntonioDueñas, José L.Calvo, Juan CarlosSánchez Margalet, VíctorVarone, Cecilia Laura17β-estradiolESTROGEN RECEPTORGENE EXPRESSIONLEPTINPLACENTAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology.Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Pérez Pérez, Antonio. Hospital Universitario Virgen Macarena; EspañaFil: Dueñas, José L.. Hospital Universitario Virgen Macarena; EspañaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Sánchez Margalet, Víctor. Hospital Universitario Virgen Macarena; EspañaFil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaElsevier Science2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93757Gambino, Yésica Paola; Pérez Pérez, Antonio; Dueñas, José L.; Calvo, Juan Carlos; Sánchez Margalet, Víctor; et al.; Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1823; 4; 4-2012; 900-9100167-4889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167488912000237info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamcr.2012.01.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:59Zoai:ri.conicet.gov.ar:11336/93757instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:00.108CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
spellingShingle Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
Gambino, Yésica Paola
17β-estradiol
ESTROGEN RECEPTOR
GENE EXPRESSION
LEPTIN
PLACENTA
title_short Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_full Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_fullStr Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_full_unstemmed Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_sort Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
dc.creator.none.fl_str_mv Gambino, Yésica Paola
Pérez Pérez, Antonio
Dueñas, José L.
Calvo, Juan Carlos
Sánchez Margalet, Víctor
Varone, Cecilia Laura
author Gambino, Yésica Paola
author_facet Gambino, Yésica Paola
Pérez Pérez, Antonio
Dueñas, José L.
Calvo, Juan Carlos
Sánchez Margalet, Víctor
Varone, Cecilia Laura
author_role author
author2 Pérez Pérez, Antonio
Dueñas, José L.
Calvo, Juan Carlos
Sánchez Margalet, Víctor
Varone, Cecilia Laura
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 17β-estradiol
ESTROGEN RECEPTOR
GENE EXPRESSION
LEPTIN
PLACENTA
topic 17β-estradiol
ESTROGEN RECEPTOR
GENE EXPRESSION
LEPTIN
PLACENTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology.
Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pérez Pérez, Antonio. Hospital Universitario Virgen Macarena; España
Fil: Dueñas, José L.. Hospital Universitario Virgen Macarena; España
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Sánchez Margalet, Víctor. Hospital Universitario Virgen Macarena; España
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93757
Gambino, Yésica Paola; Pérez Pérez, Antonio; Dueñas, José L.; Calvo, Juan Carlos; Sánchez Margalet, Víctor; et al.; Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1823; 4; 4-2012; 900-910
0167-4889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93757
identifier_str_mv Gambino, Yésica Paola; Pérez Pérez, Antonio; Dueñas, José L.; Calvo, Juan Carlos; Sánchez Margalet, Víctor; et al.; Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1823; 4; 4-2012; 900-910
0167-4889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167488912000237
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamcr.2012.01.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613707933417472
score 13.070432

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