Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
- Autores
- Gambino, Y.P.; Pérez Pérez, A.; Dueñas, J.L.; Calvo, J.C.; Sánchez-Margalet, V.; Varone, C.L.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..
Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Biochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910
- Materia
-
17β-estradiol
Estrogen receptor
Gene expression
Leptin
Placenta
estradiol
estrogen receptor alpha
leptin
mitogen activated protein kinase
protein kinase B
article
controlled study
enzyme activation
hormonal regulation
hormone action
hormone synthesis
human
human cell
placenta
priority journal
Cell Membrane
Cell Nucleus
Estradiol
Estrogen Receptor alpha
Female
Gene Expression Regulation
Gene Silencing
Humans
Leptin
Models, Biological
Placenta
Pregnancy
Protein Binding
Protein Transport
Serum Albumin, Bovine
Signal Transduction - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_01674889_v1823_n4_p900_Gambino
Ver los metadatos del registro completo
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Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alphaGambino, Y.P.Pérez Pérez, A.Dueñas, J.L.Calvo, J.C.Sánchez-Margalet, V.Varone, C.L.17β-estradiolEstrogen receptorGene expressionLeptinPlacentaestradiolestrogen receptor alphaleptinmitogen activated protein kinaseprotein kinase Barticlecontrolled studyenzyme activationhormonal regulationhormone actionhormone synthesishumanhuman cellplacentapriority journalCell MembraneCell NucleusEstradiolEstrogen Receptor alphaFemaleGene Expression RegulationGene SilencingHumansLeptinModels, BiologicalPlacentaPregnancyProtein BindingProtein TransportSerum Albumin, BovineSignal TransductionThe placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_GambinoBiochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:51Zpaperaa:paper_01674889_v1823_n4_p900_GambinoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:53.28Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
title |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
spellingShingle |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha Gambino, Y.P. 17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction |
title_short |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
title_full |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
title_fullStr |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
title_full_unstemmed |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
title_sort |
Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha |
dc.creator.none.fl_str_mv |
Gambino, Y.P. Pérez Pérez, A. Dueñas, J.L. Calvo, J.C. Sánchez-Margalet, V. Varone, C.L. |
author |
Gambino, Y.P. |
author_facet |
Gambino, Y.P. Pérez Pérez, A. Dueñas, J.L. Calvo, J.C. Sánchez-Margalet, V. Varone, C.L. |
author_role |
author |
author2 |
Pérez Pérez, A. Dueñas, J.L. Calvo, J.C. Sánchez-Margalet, V. Varone, C.L. |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction |
topic |
17β-estradiol Estrogen receptor Gene expression Leptin Placenta estradiol estrogen receptor alpha leptin mitogen activated protein kinase protein kinase B article controlled study enzyme activation hormonal regulation hormone action hormone synthesis human human cell placenta priority journal Cell Membrane Cell Nucleus Estradiol Estrogen Receptor alpha Female Gene Expression Regulation Gene Silencing Humans Leptin Models, Biological Placenta Pregnancy Protein Binding Protein Transport Serum Albumin, Bovine Signal Transduction |
dc.description.none.fl_txt_mv |
The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V.. Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V.. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino |
url |
http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Biochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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13.070432 |