Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha

Autores
Gambino, Y.P.; Pérez Pérez, A.; Dueñas, J.L.; Calvo, J.C.; Sánchez-Margalet, V.; Varone, C.L.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..
Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Biochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910
Materia
17β-estradiol
Estrogen receptor
Gene expression
Leptin
Placenta
estradiol
estrogen receptor alpha
leptin
mitogen activated protein kinase
protein kinase B
article
controlled study
enzyme activation
hormonal regulation
hormone action
hormone synthesis
human
human cell
placenta
priority journal
Cell Membrane
Cell Nucleus
Estradiol
Estrogen Receptor alpha
Female
Gene Expression Regulation
Gene Silencing
Humans
Leptin
Models, Biological
Placenta
Pregnancy
Protein Binding
Protein Transport
Serum Albumin, Bovine
Signal Transduction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_01674889_v1823_n4_p900_Gambino

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oai_identifier_str paperaa:paper_01674889_v1823_n4_p900_Gambino
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alphaGambino, Y.P.Pérez Pérez, A.Dueñas, J.L.Calvo, J.C.Sánchez-Margalet, V.Varone, C.L.17β-estradiolEstrogen receptorGene expressionLeptinPlacentaestradiolestrogen receptor alphaleptinmitogen activated protein kinaseprotein kinase Barticlecontrolled studyenzyme activationhormonal regulationhormone actionhormone synthesishumanhuman cellplacentapriority journalCell MembraneCell NucleusEstradiolEstrogen Receptor alphaFemaleGene Expression RegulationGene SilencingHumansLeptinModels, BiologicalPlacentaPregnancyProtein BindingProtein TransportSerum Albumin, BovineSignal TransductionThe placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_GambinoBiochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:51Zpaperaa:paper_01674889_v1823_n4_p900_GambinoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:53.28Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
spellingShingle Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
Gambino, Y.P.
17β-estradiol
Estrogen receptor
Gene expression
Leptin
Placenta
estradiol
estrogen receptor alpha
leptin
mitogen activated protein kinase
protein kinase B
article
controlled study
enzyme activation
hormonal regulation
hormone action
hormone synthesis
human
human cell
placenta
priority journal
Cell Membrane
Cell Nucleus
Estradiol
Estrogen Receptor alpha
Female
Gene Expression Regulation
Gene Silencing
Humans
Leptin
Models, Biological
Placenta
Pregnancy
Protein Binding
Protein Transport
Serum Albumin, Bovine
Signal Transduction
title_short Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_full Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_fullStr Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_full_unstemmed Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
title_sort Regulation of leptin expression by 17beta-estradiol in human placental cells involves membrane associated estrogen receptor alpha
dc.creator.none.fl_str_mv Gambino, Y.P.
Pérez Pérez, A.
Dueñas, J.L.
Calvo, J.C.
Sánchez-Margalet, V.
Varone, C.L.
author Gambino, Y.P.
author_facet Gambino, Y.P.
Pérez Pérez, A.
Dueñas, J.L.
Calvo, J.C.
Sánchez-Margalet, V.
Varone, C.L.
author_role author
author2 Pérez Pérez, A.
Dueñas, J.L.
Calvo, J.C.
Sánchez-Margalet, V.
Varone, C.L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 17β-estradiol
Estrogen receptor
Gene expression
Leptin
Placenta
estradiol
estrogen receptor alpha
leptin
mitogen activated protein kinase
protein kinase B
article
controlled study
enzyme activation
hormonal regulation
hormone action
hormone synthesis
human
human cell
placenta
priority journal
Cell Membrane
Cell Nucleus
Estradiol
Estrogen Receptor alpha
Female
Gene Expression Regulation
Gene Silencing
Humans
Leptin
Models, Biological
Placenta
Pregnancy
Protein Binding
Protein Transport
Serum Albumin, Bovine
Signal Transduction
topic 17β-estradiol
Estrogen receptor
Gene expression
Leptin
Placenta
estradiol
estrogen receptor alpha
leptin
mitogen activated protein kinase
protein kinase B
article
controlled study
enzyme activation
hormonal regulation
hormone action
hormone synthesis
human
human cell
placenta
priority journal
Cell Membrane
Cell Nucleus
Estradiol
Estrogen Receptor alpha
Female
Gene Expression Regulation
Gene Silencing
Humans
Leptin
Models, Biological
Placenta
Pregnancy
Protein Binding
Protein Transport
Serum Albumin, Bovine
Signal Transduction
dc.description.none.fl_txt_mv The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..
Fil:Gambino, Y.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Varone, C.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The placenta produces a wide number of molecules that play essential roles in the establishment and maintenance of pregnancy. In this context, leptin has emerged as an important player in reproduction. The synthesis of leptin in normal trophoblastic cells is regulated by different endogenous biochemical agents, but the regulation of placental leptin expression is still poorly understood. We have previously reported that 17β-estradiol (E 2) up-regulates placental leptin expression. To improve the understanding of estrogen receptor mechanisms in regulating leptin gene expression, in the current study we examined the effect of membrane-constrained E 2 conjugate, E-BSA, on leptin expression in human placental cells. We have found that leptin expression was induced by E-BSA both in BeWo cells and human placental explants, suggesting that E 2 also exerts its effects through membrane receptors. Moreover E-BSA rapidly activated different MAPKs and AKT pathways, and these pathways were involved in E 2 induced placental leptin expression. On the other hand we demonstrated the presence of ERα associated to the plasma membrane of BeWo cells. We showed that E 2 genomic and nongenomic actions could be mediated by ERα. Supporting this idea, the downregulation of ERα level through a specific siRNA, decreased E-BSA effects on leptin expression. Taken together, these results provide new evidence of the mechanisms whereby E 2 regulates leptin expression in placenta and support the importance of leptin in placental physiology. © 2012 Elsevier B.V..
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino
url http://hdl.handle.net/20.500.12110/paper_01674889_v1823_n4_p900_Gambino
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biochim. Biophys. Acta Mol. Cell Res. 2012;1823(4):900-910
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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