The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication

Autores
Brunetti, Jesús Emanuel; Scolaro, Luis Alberto; Castilla, Viviana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengue virus type 2 (DENV-2) and Junín virus (JUNV). We determined that DENV-2 promoted the cytoplasmic translocation of hnRNP K and to a lesser extent of hnRNP A2, meanwhile, JUNV infection induced an increase in hnRNP K cytoplasmic localization whereas hnRNP A2 remained mainly in the nucleus of infected cells. Both hnRNP K and hnRNP A2 were localized predominantly in the nucleus of JUNV persistently infected cells even after superinfection with JUNV indicating that persistent infection does not alter nucleo-cytoplasmic transport of these hnRNPs. Total levels of hnRNP K expression were unaffected by DENV-2 or JUNV infection. In addition we determined, using small interfering RNAs, that hnRNP K knockout inhibits DENV-2 and JUNV multiplication. Our results indicate that DENV-2 and JUNV induce hnRNPK cytoplasmic translocation to favor viral multiplication.
Fil: Brunetti, Jesús Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Scolaro, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Materia
Dengue Virus
Junin Virus
Heterogeneous Nuclear Ribonucleoproteins
Hnrnp A2
Hnrnp K
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43412
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43412
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplicationBrunetti, Jesús EmanuelScolaro, Luis AlbertoCastilla, VivianaDengue VirusJunin VirusHeterogeneous Nuclear RibonucleoproteinsHnrnp A2Hnrnp Khttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengue virus type 2 (DENV-2) and Junín virus (JUNV). We determined that DENV-2 promoted the cytoplasmic translocation of hnRNP K and to a lesser extent of hnRNP A2, meanwhile, JUNV infection induced an increase in hnRNP K cytoplasmic localization whereas hnRNP A2 remained mainly in the nucleus of infected cells. Both hnRNP K and hnRNP A2 were localized predominantly in the nucleus of JUNV persistently infected cells even after superinfection with JUNV indicating that persistent infection does not alter nucleo-cytoplasmic transport of these hnRNPs. Total levels of hnRNP K expression were unaffected by DENV-2 or JUNV infection. In addition we determined, using small interfering RNAs, that hnRNP K knockout inhibits DENV-2 and JUNV multiplication. Our results indicate that DENV-2 and JUNV induce hnRNPK cytoplasmic translocation to favor viral multiplication.Fil: Brunetti, Jesús Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Scolaro, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaElsevier Science2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43412Brunetti, Jesús Emanuel; Scolaro, Luis Alberto; Castilla, Viviana; The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication; Elsevier Science; Virus Research; 203; 5-2015; 84-910168-17021872-7492CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2015.04.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168170215001355info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:49:40Zoai:ri.conicet.gov.ar:11336/43412instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:49:41.131CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
title The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
spellingShingle The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
Brunetti, Jesús Emanuel
Dengue Virus
Junin Virus
Heterogeneous Nuclear Ribonucleoproteins
Hnrnp A2
Hnrnp K
title_short The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
title_full The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
title_fullStr The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
title_full_unstemmed The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
title_sort The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication
dc.creator.none.fl_str_mv Brunetti, Jesús Emanuel
Scolaro, Luis Alberto
Castilla, Viviana
author Brunetti, Jesús Emanuel
author_facet Brunetti, Jesús Emanuel
Scolaro, Luis Alberto
Castilla, Viviana
author_role author
author2 Scolaro, Luis Alberto
Castilla, Viviana
author2_role author
author
dc.subject.none.fl_str_mv Dengue Virus
Junin Virus
Heterogeneous Nuclear Ribonucleoproteins
Hnrnp A2
Hnrnp K
topic Dengue Virus
Junin Virus
Heterogeneous Nuclear Ribonucleoproteins
Hnrnp A2
Hnrnp K
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengue virus type 2 (DENV-2) and Junín virus (JUNV). We determined that DENV-2 promoted the cytoplasmic translocation of hnRNP K and to a lesser extent of hnRNP A2, meanwhile, JUNV infection induced an increase in hnRNP K cytoplasmic localization whereas hnRNP A2 remained mainly in the nucleus of infected cells. Both hnRNP K and hnRNP A2 were localized predominantly in the nucleus of JUNV persistently infected cells even after superinfection with JUNV indicating that persistent infection does not alter nucleo-cytoplasmic transport of these hnRNPs. Total levels of hnRNP K expression were unaffected by DENV-2 or JUNV infection. In addition we determined, using small interfering RNAs, that hnRNP K knockout inhibits DENV-2 and JUNV multiplication. Our results indicate that DENV-2 and JUNV induce hnRNPK cytoplasmic translocation to favor viral multiplication.
Fil: Brunetti, Jesús Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Scolaro, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Castilla, Viviana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
description Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengue virus type 2 (DENV-2) and Junín virus (JUNV). We determined that DENV-2 promoted the cytoplasmic translocation of hnRNP K and to a lesser extent of hnRNP A2, meanwhile, JUNV infection induced an increase in hnRNP K cytoplasmic localization whereas hnRNP A2 remained mainly in the nucleus of infected cells. Both hnRNP K and hnRNP A2 were localized predominantly in the nucleus of JUNV persistently infected cells even after superinfection with JUNV indicating that persistent infection does not alter nucleo-cytoplasmic transport of these hnRNPs. Total levels of hnRNP K expression were unaffected by DENV-2 or JUNV infection. In addition we determined, using small interfering RNAs, that hnRNP K knockout inhibits DENV-2 and JUNV multiplication. Our results indicate that DENV-2 and JUNV induce hnRNPK cytoplasmic translocation to favor viral multiplication.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43412
Brunetti, Jesús Emanuel; Scolaro, Luis Alberto; Castilla, Viviana; The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication; Elsevier Science; Virus Research; 203; 5-2015; 84-91
0168-1702
1872-7492
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43412
identifier_str_mv Brunetti, Jesús Emanuel; Scolaro, Luis Alberto; Castilla, Viviana; The heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a host factor required for dengue virus and Junin virus multiplication; Elsevier Science; Virus Research; 203; 5-2015; 84-91
0168-1702
1872-7492
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2015.04.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168170215001355
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.121305

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