Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes

Autores
Grune Loffler, Sylvia; Samartino, Luis Ernesto; Brihuega, Bibiana
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Leptospirosis is the most widespread zoonosis worldwide. This disease is caused by bacteria, pathogenic strains of the genus Leptospira spp. belonging to the order Spirochaetales. These pathogenic strains of Leptospira spp. are disseminated through urine of infected animals and/or reservoirs into the environment. Human and animal vaccines are not available in many countries and are serovar specific. It is very important to study the genotypes of pathogenic leptosprial strains, since this allows knowing the current epidemiological scenario from endemic countries and in high risk areas. Methods and findings: A total of 67 pathogenic Leptospira spp. strains were used in this study. Genotyping using Multiple Locus Variable-number tandem repeat Analysis (MLVA) was carried out. The discriminatory power of two sets of loci (Set A: VNTRs 4, 7, 9, 10, 19, 23, 31 and Set B: VNTRs: 4bis, 7bis, 10bis, Lb4, Lb5) was analyzed by Principal Coordinate Analysis (PCoA). This type of analysis has not been previously applied to bacterial genotypes. PCoA was done using a distance matrix of the genotypes obtained and the genetic variability was analyzed. According to the numbers of alleles found, it was possible to conclude that the discriminatory power of the five (5) loci of VNTR Set B is higher than that of the seven (7) loci of VNTR Set A. The discriminatory power of VNTR Lb5 and VNTR 31 is very high, since with one different allele the genotypes characterized in this study could be separated. Conclusion: In this study the use of PCoA was very helpful to analyse the obtained pathogenic Leptospira spp. genotypes isolated from animals, humans and the environment. This analysis gave a current results compared with recent studies that include the evaluation of the discriminatory power of VNTRs for genotyping of pathogenic leptospiral strains. The VNTR Lb5 and VNTR 31 have the greatest power of discrimination between serovars.
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Materia
LEPTOSPIROSIS
PATHOGENIC
MLVA
GENOTYPES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78470
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78470
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypesGrune Loffler, SylviaSamartino, Luis ErnestoBrihuega, BibianaLEPTOSPIROSISPATHOGENICMLVAGENOTYPEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Leptospirosis is the most widespread zoonosis worldwide. This disease is caused by bacteria, pathogenic strains of the genus Leptospira spp. belonging to the order Spirochaetales. These pathogenic strains of Leptospira spp. are disseminated through urine of infected animals and/or reservoirs into the environment. Human and animal vaccines are not available in many countries and are serovar specific. It is very important to study the genotypes of pathogenic leptosprial strains, since this allows knowing the current epidemiological scenario from endemic countries and in high risk areas. Methods and findings: A total of 67 pathogenic Leptospira spp. strains were used in this study. Genotyping using Multiple Locus Variable-number tandem repeat Analysis (MLVA) was carried out. The discriminatory power of two sets of loci (Set A: VNTRs 4, 7, 9, 10, 19, 23, 31 and Set B: VNTRs: 4bis, 7bis, 10bis, Lb4, Lb5) was analyzed by Principal Coordinate Analysis (PCoA). This type of analysis has not been previously applied to bacterial genotypes. PCoA was done using a distance matrix of the genotypes obtained and the genetic variability was analyzed. According to the numbers of alleles found, it was possible to conclude that the discriminatory power of the five (5) loci of VNTR Set B is higher than that of the seven (7) loci of VNTR Set A. The discriminatory power of VNTR Lb5 and VNTR 31 is very high, since with one different allele the genotypes characterized in this study could be separated. Conclusion: In this study the use of PCoA was very helpful to analyse the obtained pathogenic Leptospira spp. genotypes isolated from animals, humans and the environment. This analysis gave a current results compared with recent studies that include the evaluation of the discriminatory power of VNTRs for genotyping of pathogenic leptospiral strains. The VNTR Lb5 and VNTR 31 have the greatest power of discrimination between serovars.Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaiMedPub2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78470Grune Loffler, Sylvia; Samartino, Luis Ernesto; Brihuega, Bibiana; Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes; iMedPub; Journal of Zoonotic Diseases and Public Health; 1; 1; 1-2017; 1-80093-3821CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.imedpub.com/articles/insights-into-genetic-distances-of-pathogenic-leptospira-spp-from-humans-animals-and-environment-using-multiple-locus-variablenumb.php?aid=18087info:eu-repo/semantics/altIdentifier/url/http://www.imedpub.com/articles/insights-into-genetic-distances-of-pathogenic-leptospira-spp-from-humans-animals-and-environment-using-multiple-locus-variablenumb.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:32Zoai:ri.conicet.gov.ar:11336/78470instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:32.826CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
title Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
spellingShingle Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
Grune Loffler, Sylvia
LEPTOSPIROSIS
PATHOGENIC
MLVA
GENOTYPES
title_short Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
title_full Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
title_fullStr Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
title_full_unstemmed Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
title_sort Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes
dc.creator.none.fl_str_mv Grune Loffler, Sylvia
Samartino, Luis Ernesto
Brihuega, Bibiana
author Grune Loffler, Sylvia
author_facet Grune Loffler, Sylvia
Samartino, Luis Ernesto
Brihuega, Bibiana
author_role author
author2 Samartino, Luis Ernesto
Brihuega, Bibiana
author2_role author
author
dc.subject.none.fl_str_mv LEPTOSPIROSIS
PATHOGENIC
MLVA
GENOTYPES
topic LEPTOSPIROSIS
PATHOGENIC
MLVA
GENOTYPES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Leptospirosis is the most widespread zoonosis worldwide. This disease is caused by bacteria, pathogenic strains of the genus Leptospira spp. belonging to the order Spirochaetales. These pathogenic strains of Leptospira spp. are disseminated through urine of infected animals and/or reservoirs into the environment. Human and animal vaccines are not available in many countries and are serovar specific. It is very important to study the genotypes of pathogenic leptosprial strains, since this allows knowing the current epidemiological scenario from endemic countries and in high risk areas. Methods and findings: A total of 67 pathogenic Leptospira spp. strains were used in this study. Genotyping using Multiple Locus Variable-number tandem repeat Analysis (MLVA) was carried out. The discriminatory power of two sets of loci (Set A: VNTRs 4, 7, 9, 10, 19, 23, 31 and Set B: VNTRs: 4bis, 7bis, 10bis, Lb4, Lb5) was analyzed by Principal Coordinate Analysis (PCoA). This type of analysis has not been previously applied to bacterial genotypes. PCoA was done using a distance matrix of the genotypes obtained and the genetic variability was analyzed. According to the numbers of alleles found, it was possible to conclude that the discriminatory power of the five (5) loci of VNTR Set B is higher than that of the seven (7) loci of VNTR Set A. The discriminatory power of VNTR Lb5 and VNTR 31 is very high, since with one different allele the genotypes characterized in this study could be separated. Conclusion: In this study the use of PCoA was very helpful to analyse the obtained pathogenic Leptospira spp. genotypes isolated from animals, humans and the environment. This analysis gave a current results compared with recent studies that include the evaluation of the discriminatory power of VNTRs for genotyping of pathogenic leptospiral strains. The VNTR Lb5 and VNTR 31 have the greatest power of discrimination between serovars.
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
description Background: Leptospirosis is the most widespread zoonosis worldwide. This disease is caused by bacteria, pathogenic strains of the genus Leptospira spp. belonging to the order Spirochaetales. These pathogenic strains of Leptospira spp. are disseminated through urine of infected animals and/or reservoirs into the environment. Human and animal vaccines are not available in many countries and are serovar specific. It is very important to study the genotypes of pathogenic leptosprial strains, since this allows knowing the current epidemiological scenario from endemic countries and in high risk areas. Methods and findings: A total of 67 pathogenic Leptospira spp. strains were used in this study. Genotyping using Multiple Locus Variable-number tandem repeat Analysis (MLVA) was carried out. The discriminatory power of two sets of loci (Set A: VNTRs 4, 7, 9, 10, 19, 23, 31 and Set B: VNTRs: 4bis, 7bis, 10bis, Lb4, Lb5) was analyzed by Principal Coordinate Analysis (PCoA). This type of analysis has not been previously applied to bacterial genotypes. PCoA was done using a distance matrix of the genotypes obtained and the genetic variability was analyzed. According to the numbers of alleles found, it was possible to conclude that the discriminatory power of the five (5) loci of VNTR Set B is higher than that of the seven (7) loci of VNTR Set A. The discriminatory power of VNTR Lb5 and VNTR 31 is very high, since with one different allele the genotypes characterized in this study could be separated. Conclusion: In this study the use of PCoA was very helpful to analyse the obtained pathogenic Leptospira spp. genotypes isolated from animals, humans and the environment. This analysis gave a current results compared with recent studies that include the evaluation of the discriminatory power of VNTRs for genotyping of pathogenic leptospiral strains. The VNTR Lb5 and VNTR 31 have the greatest power of discrimination between serovars.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78470
Grune Loffler, Sylvia; Samartino, Luis Ernesto; Brihuega, Bibiana; Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes; iMedPub; Journal of Zoonotic Diseases and Public Health; 1; 1; 1-2017; 1-8
0093-3821
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78470
identifier_str_mv Grune Loffler, Sylvia; Samartino, Luis Ernesto; Brihuega, Bibiana; Insights into genetic distances of pathogenic Leptospira spp. from humans, animals and environment using Multiple Locus Variable-number tandem repeat Analysis (MLVA) genotypes; iMedPub; Journal of Zoonotic Diseases and Public Health; 1; 1; 1-2017; 1-8
0093-3821
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.imedpub.com/articles/insights-into-genetic-distances-of-pathogenic-leptospira-spp-from-humans-animals-and-environment-using-multiple-locus-variablenumb.php?aid=18087
info:eu-repo/semantics/altIdentifier/url/http://www.imedpub.com/articles/insights-into-genetic-distances-of-pathogenic-leptospira-spp-from-humans-animals-and-environment-using-multiple-locus-variablenumb.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv iMedPub
publisher.none.fl_str_mv iMedPub
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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