Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions

Autores
Cardoso, Renato M. S.; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal; Loura, Luís M. S.; Moreno, Maria João
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes is very relevant. In this work, the dipole potentials of monolayers, formed at the air-water interface, from pure or mixed lipids (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyserine (POPS), sphingomyelin (SpM) and cholesterol) were measured and correlated with the mean area per lipid. The results showed that, as previously observed, cholesterol increases the dipole potential in correspondence with the decrease in the average area per lipid. At the small mole fractions encountered in biomembranes, the presence of the negatively charged lipid POPS increases the dipole potentials of monolayers despite inducing an increase in the average area per lipid. Additionally, the inclusion of POPE in POPC:cholesterol monolayers disrupts the area condensation induced by cholesterol while increasing the membrane dipole moment, leading to a small reduction in the dipole potential. This trend is reinforced for the quaternary POPC:cholesterol:POPE:POPS 4:3:2:1 system, which mimics the inner leaflets of eukaryotic plasma membranes. In agreement with previous works, the replacement of phosphocholine lipids with sphingomyelin leads to a decrease in the dipole potential. Together, this results in a lower dipole potential for the SpM-enriched outer leaflet, generating a non-zero transbilayer dipole potential in the asymmetric plasma membranes of eukaryotic cells.
Fil: Cardoso, Renato M. S.. Universidad de Coimbra; Portugal
Fil: Lairion, Fabiana Norma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Fil: Loura, Luís M. S.. Universidad de Coimbra; Portugal
Fil: Moreno, Maria João. Universidad de Coimbra; Portugal
Materia
POPC
LIPIDS
CHOLESTEROL
AREA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276138
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276138
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dipole Potential of Monolayers with Biologically Relevant Lipid CompositionsCardoso, Renato M. S.Lairion, Fabiana NormaDisalvo, Edgardo AnibalLoura, Luís M. S.Moreno, Maria JoãoPOPCLIPIDSCHOLESTEROLAREAhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes is very relevant. In this work, the dipole potentials of monolayers, formed at the air-water interface, from pure or mixed lipids (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyserine (POPS), sphingomyelin (SpM) and cholesterol) were measured and correlated with the mean area per lipid. The results showed that, as previously observed, cholesterol increases the dipole potential in correspondence with the decrease in the average area per lipid. At the small mole fractions encountered in biomembranes, the presence of the negatively charged lipid POPS increases the dipole potentials of monolayers despite inducing an increase in the average area per lipid. Additionally, the inclusion of POPE in POPC:cholesterol monolayers disrupts the area condensation induced by cholesterol while increasing the membrane dipole moment, leading to a small reduction in the dipole potential. This trend is reinforced for the quaternary POPC:cholesterol:POPE:POPS 4:3:2:1 system, which mimics the inner leaflets of eukaryotic plasma membranes. In agreement with previous works, the replacement of phosphocholine lipids with sphingomyelin leads to a decrease in the dipole potential. Together, this results in a lower dipole potential for the SpM-enriched outer leaflet, generating a non-zero transbilayer dipole potential in the asymmetric plasma membranes of eukaryotic cells.Fil: Cardoso, Renato M. S.. Universidad de Coimbra; PortugalFil: Lairion, Fabiana Norma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; ArgentinaFil: Loura, Luís M. S.. Universidad de Coimbra; PortugalFil: Moreno, Maria João. Universidad de Coimbra; PortugalMultidisciplinary Digital Publishing Institute2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276138Cardoso, Renato M. S.; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal; Loura, Luís M. S.; Moreno, Maria João; Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions; Multidisciplinary Digital Publishing Institute; Molecules; 29; 24; 12-2024; 1-191420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/29/24/5843info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules29245843info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:29:10Zoai:ri.conicet.gov.ar:11336/276138instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:29:10.884CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
title Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
spellingShingle Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
Cardoso, Renato M. S.
POPC
LIPIDS
CHOLESTEROL
AREA
title_short Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
title_full Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
title_fullStr Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
title_full_unstemmed Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
title_sort Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions
dc.creator.none.fl_str_mv Cardoso, Renato M. S.
Lairion, Fabiana Norma
Disalvo, Edgardo Anibal
Loura, Luís M. S.
Moreno, Maria João
author Cardoso, Renato M. S.
author_facet Cardoso, Renato M. S.
Lairion, Fabiana Norma
Disalvo, Edgardo Anibal
Loura, Luís M. S.
Moreno, Maria João
author_role author
author2 Lairion, Fabiana Norma
Disalvo, Edgardo Anibal
Loura, Luís M. S.
Moreno, Maria João
author2_role author
author
author
author
dc.subject.none.fl_str_mv POPC
LIPIDS
CHOLESTEROL
AREA
topic POPC
LIPIDS
CHOLESTEROL
AREA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes is very relevant. In this work, the dipole potentials of monolayers, formed at the air-water interface, from pure or mixed lipids (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyserine (POPS), sphingomyelin (SpM) and cholesterol) were measured and correlated with the mean area per lipid. The results showed that, as previously observed, cholesterol increases the dipole potential in correspondence with the decrease in the average area per lipid. At the small mole fractions encountered in biomembranes, the presence of the negatively charged lipid POPS increases the dipole potentials of monolayers despite inducing an increase in the average area per lipid. Additionally, the inclusion of POPE in POPC:cholesterol monolayers disrupts the area condensation induced by cholesterol while increasing the membrane dipole moment, leading to a small reduction in the dipole potential. This trend is reinforced for the quaternary POPC:cholesterol:POPE:POPS 4:3:2:1 system, which mimics the inner leaflets of eukaryotic plasma membranes. In agreement with previous works, the replacement of phosphocholine lipids with sphingomyelin leads to a decrease in the dipole potential. Together, this results in a lower dipole potential for the SpM-enriched outer leaflet, generating a non-zero transbilayer dipole potential in the asymmetric plasma membranes of eukaryotic cells.
Fil: Cardoso, Renato M. S.. Universidad de Coimbra; Portugal
Fil: Lairion, Fabiana Norma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Fil: Loura, Luís M. S.. Universidad de Coimbra; Portugal
Fil: Moreno, Maria João. Universidad de Coimbra; Portugal
description The membrane dipole potential that arises from the interfacial water and constitutive dipolar groups of lipid molecules modulates the interaction of amphiphiles and proteins with membranes. Consequently, its determination for lipid mixtures resembling the existing diversity in biological membranes is very relevant. In this work, the dipole potentials of monolayers, formed at the air-water interface, from pure or mixed lipids (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyserine (POPS), sphingomyelin (SpM) and cholesterol) were measured and correlated with the mean area per lipid. The results showed that, as previously observed, cholesterol increases the dipole potential in correspondence with the decrease in the average area per lipid. At the small mole fractions encountered in biomembranes, the presence of the negatively charged lipid POPS increases the dipole potentials of monolayers despite inducing an increase in the average area per lipid. Additionally, the inclusion of POPE in POPC:cholesterol monolayers disrupts the area condensation induced by cholesterol while increasing the membrane dipole moment, leading to a small reduction in the dipole potential. This trend is reinforced for the quaternary POPC:cholesterol:POPE:POPS 4:3:2:1 system, which mimics the inner leaflets of eukaryotic plasma membranes. In agreement with previous works, the replacement of phosphocholine lipids with sphingomyelin leads to a decrease in the dipole potential. Together, this results in a lower dipole potential for the SpM-enriched outer leaflet, generating a non-zero transbilayer dipole potential in the asymmetric plasma membranes of eukaryotic cells.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276138
Cardoso, Renato M. S.; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal; Loura, Luís M. S.; Moreno, Maria João; Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions; Multidisciplinary Digital Publishing Institute; Molecules; 29; 24; 12-2024; 1-19
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276138
identifier_str_mv Cardoso, Renato M. S.; Lairion, Fabiana Norma; Disalvo, Edgardo Anibal; Loura, Luís M. S.; Moreno, Maria João; Dipole Potential of Monolayers with Biologically Relevant Lipid Compositions; Multidisciplinary Digital Publishing Institute; Molecules; 29; 24; 12-2024; 1-19
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/29/24/5843
info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules29245843
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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