Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia

Autores
Althabegoiti, Maria Julia; Ormeño Orrillo, Ernesto; Lozano, Luis; Torres Tejerizo, Gonzalo Arturo; Rogel, Marco Antonio; Mora, Jaime; Martinez Romero, Esperanza
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Rhizobium grahamii belongs to a new phylogenetic group of rhizobia together with Rhizobium mesoamericanum and other species. R. grahamii has a broad-host-range that includes Leucaena leucocephala and Phaseolus vulgaris, although it is a poor competitor for P. vulgaris nodulation in the presence of Rhizobium etli or Rhizobium phaseoli strains. This work analyzed the genome sequence and transfer properties of R. grahamii plasmids. Results Genome sequence was obtained from R. grahamii CCGE502 type strain isolated from Dalea leporina in Mexico. The CCGE502 genome comprises one chromosome and two extrachromosomal replicons (ERs), pRgrCCGE502a and pRgrCCGE502b. Additionally, a plasmid integrated in the CCGE502 chromosome was found. The genomic comparison of ERs from this group showed that gene content is more variable than average nucleotide identity (ANI). Well conserved nod and nif genes were found in R. grahamii and R. mesoamericanum with some differences. R. phaseoli Ch24-10 genes expressed in bacterial cells in roots were found to be conserved in pRgrCCGE502b. Regarding conjugative transfer we were unable to transfer the R. grahamii CCGE502 symbiotic plasmid and its megaplasmid to other rhizobial hosts but we could transfer the symbiotic plasmid to Agrobacterium tumefaciens with transfer dependent on homoserine lactones. Conclusion Variable degrees of nucleotide identity and gene content conservation were found among the different R. grahamii CCGE502 replicons in comparison to R. mesoamericanum genomes. The extrachromosomal replicons from R. grahamii were more similar to those found in phylogenetically related Rhizobium species. However, limited similarities of R. grahamii CCGE502 symbiotic plasmid and megaplasmid were observed in other more distant Rhizobium species. The set of conserved genes in R. grahamii comprises some of those that are highly expressed in R. phaseoli on plant roots, suggesting that they play an important role in root colonization.
Fil: Althabegoiti, Maria Julia. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ormeño Orrillo, Ernesto. Universidad Nacional Autónoma de México; México
Fil: Lozano, Luis. Universidad Nacional Autónoma de México; México
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional Autónoma de México; México
Fil: Rogel, Marco Antonio. Universidad Nacional Autónoma de México; México
Fil: Mora, Jaime. Universidad Nacional Autónoma de México; México
Fil: Martinez Romero, Esperanza. Universidad Nacional Autónoma de México; México
Materia
Genome sequence
Quorum sensing
Conjugative transfer
Symbiotic plasmid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32348
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32348
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobiaAlthabegoiti, Maria JuliaOrmeño Orrillo, ErnestoLozano, LuisTorres Tejerizo, Gonzalo ArturoRogel, Marco AntonioMora, JaimeMartinez Romero, EsperanzaGenome sequenceQuorum sensingConjugative transferSymbiotic plasmidhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background Rhizobium grahamii belongs to a new phylogenetic group of rhizobia together with Rhizobium mesoamericanum and other species. R. grahamii has a broad-host-range that includes Leucaena leucocephala and Phaseolus vulgaris, although it is a poor competitor for P. vulgaris nodulation in the presence of Rhizobium etli or Rhizobium phaseoli strains. This work analyzed the genome sequence and transfer properties of R. grahamii plasmids. Results Genome sequence was obtained from R. grahamii CCGE502 type strain isolated from Dalea leporina in Mexico. The CCGE502 genome comprises one chromosome and two extrachromosomal replicons (ERs), pRgrCCGE502a and pRgrCCGE502b. Additionally, a plasmid integrated in the CCGE502 chromosome was found. The genomic comparison of ERs from this group showed that gene content is more variable than average nucleotide identity (ANI). Well conserved nod and nif genes were found in R. grahamii and R. mesoamericanum with some differences. R. phaseoli Ch24-10 genes expressed in bacterial cells in roots were found to be conserved in pRgrCCGE502b. Regarding conjugative transfer we were unable to transfer the R. grahamii CCGE502 symbiotic plasmid and its megaplasmid to other rhizobial hosts but we could transfer the symbiotic plasmid to Agrobacterium tumefaciens with transfer dependent on homoserine lactones. Conclusion Variable degrees of nucleotide identity and gene content conservation were found among the different R. grahamii CCGE502 replicons in comparison to R. mesoamericanum genomes. The extrachromosomal replicons from R. grahamii were more similar to those found in phylogenetically related Rhizobium species. However, limited similarities of R. grahamii CCGE502 symbiotic plasmid and megaplasmid were observed in other more distant Rhizobium species. The set of conserved genes in R. grahamii comprises some of those that are highly expressed in R. phaseoli on plant roots, suggesting that they play an important role in root colonization.Fil: Althabegoiti, Maria Julia. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ormeño Orrillo, Ernesto. Universidad Nacional Autónoma de México; MéxicoFil: Lozano, Luis. Universidad Nacional Autónoma de México; MéxicoFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional Autónoma de México; MéxicoFil: Rogel, Marco Antonio. Universidad Nacional Autónoma de México; MéxicoFil: Mora, Jaime. Universidad Nacional Autónoma de México; MéxicoFil: Martinez Romero, Esperanza. Universidad Nacional Autónoma de México; MéxicoBioMed Central2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32348Martinez Romero, Esperanza; Mora, Jaime; Rogel, Marco Antonio; Torres Tejerizo, Gonzalo Arturo; Lozano, Luis; Ormeño Orrillo, Ernesto; et al.; Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia; BioMed Central; Bmc Microbiology; 14; 6; 1-2014; 1-101471-2180CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-6info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-14-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:06Zoai:ri.conicet.gov.ar:11336/32348instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:30:06.944CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
title Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
spellingShingle Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
Althabegoiti, Maria Julia
Genome sequence
Quorum sensing
Conjugative transfer
Symbiotic plasmid
title_short Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
title_full Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
title_fullStr Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
title_full_unstemmed Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
title_sort Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia
dc.creator.none.fl_str_mv Althabegoiti, Maria Julia
Ormeño Orrillo, Ernesto
Lozano, Luis
Torres Tejerizo, Gonzalo Arturo
Rogel, Marco Antonio
Mora, Jaime
Martinez Romero, Esperanza
author Althabegoiti, Maria Julia
author_facet Althabegoiti, Maria Julia
Ormeño Orrillo, Ernesto
Lozano, Luis
Torres Tejerizo, Gonzalo Arturo
Rogel, Marco Antonio
Mora, Jaime
Martinez Romero, Esperanza
author_role author
author2 Ormeño Orrillo, Ernesto
Lozano, Luis
Torres Tejerizo, Gonzalo Arturo
Rogel, Marco Antonio
Mora, Jaime
Martinez Romero, Esperanza
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Genome sequence
Quorum sensing
Conjugative transfer
Symbiotic plasmid
topic Genome sequence
Quorum sensing
Conjugative transfer
Symbiotic plasmid
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background Rhizobium grahamii belongs to a new phylogenetic group of rhizobia together with Rhizobium mesoamericanum and other species. R. grahamii has a broad-host-range that includes Leucaena leucocephala and Phaseolus vulgaris, although it is a poor competitor for P. vulgaris nodulation in the presence of Rhizobium etli or Rhizobium phaseoli strains. This work analyzed the genome sequence and transfer properties of R. grahamii plasmids. Results Genome sequence was obtained from R. grahamii CCGE502 type strain isolated from Dalea leporina in Mexico. The CCGE502 genome comprises one chromosome and two extrachromosomal replicons (ERs), pRgrCCGE502a and pRgrCCGE502b. Additionally, a plasmid integrated in the CCGE502 chromosome was found. The genomic comparison of ERs from this group showed that gene content is more variable than average nucleotide identity (ANI). Well conserved nod and nif genes were found in R. grahamii and R. mesoamericanum with some differences. R. phaseoli Ch24-10 genes expressed in bacterial cells in roots were found to be conserved in pRgrCCGE502b. Regarding conjugative transfer we were unable to transfer the R. grahamii CCGE502 symbiotic plasmid and its megaplasmid to other rhizobial hosts but we could transfer the symbiotic plasmid to Agrobacterium tumefaciens with transfer dependent on homoserine lactones. Conclusion Variable degrees of nucleotide identity and gene content conservation were found among the different R. grahamii CCGE502 replicons in comparison to R. mesoamericanum genomes. The extrachromosomal replicons from R. grahamii were more similar to those found in phylogenetically related Rhizobium species. However, limited similarities of R. grahamii CCGE502 symbiotic plasmid and megaplasmid were observed in other more distant Rhizobium species. The set of conserved genes in R. grahamii comprises some of those that are highly expressed in R. phaseoli on plant roots, suggesting that they play an important role in root colonization.
Fil: Althabegoiti, Maria Julia. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ormeño Orrillo, Ernesto. Universidad Nacional Autónoma de México; México
Fil: Lozano, Luis. Universidad Nacional Autónoma de México; México
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional Autónoma de México; México
Fil: Rogel, Marco Antonio. Universidad Nacional Autónoma de México; México
Fil: Mora, Jaime. Universidad Nacional Autónoma de México; México
Fil: Martinez Romero, Esperanza. Universidad Nacional Autónoma de México; México
description Background Rhizobium grahamii belongs to a new phylogenetic group of rhizobia together with Rhizobium mesoamericanum and other species. R. grahamii has a broad-host-range that includes Leucaena leucocephala and Phaseolus vulgaris, although it is a poor competitor for P. vulgaris nodulation in the presence of Rhizobium etli or Rhizobium phaseoli strains. This work analyzed the genome sequence and transfer properties of R. grahamii plasmids. Results Genome sequence was obtained from R. grahamii CCGE502 type strain isolated from Dalea leporina in Mexico. The CCGE502 genome comprises one chromosome and two extrachromosomal replicons (ERs), pRgrCCGE502a and pRgrCCGE502b. Additionally, a plasmid integrated in the CCGE502 chromosome was found. The genomic comparison of ERs from this group showed that gene content is more variable than average nucleotide identity (ANI). Well conserved nod and nif genes were found in R. grahamii and R. mesoamericanum with some differences. R. phaseoli Ch24-10 genes expressed in bacterial cells in roots were found to be conserved in pRgrCCGE502b. Regarding conjugative transfer we were unable to transfer the R. grahamii CCGE502 symbiotic plasmid and its megaplasmid to other rhizobial hosts but we could transfer the symbiotic plasmid to Agrobacterium tumefaciens with transfer dependent on homoserine lactones. Conclusion Variable degrees of nucleotide identity and gene content conservation were found among the different R. grahamii CCGE502 replicons in comparison to R. mesoamericanum genomes. The extrachromosomal replicons from R. grahamii were more similar to those found in phylogenetically related Rhizobium species. However, limited similarities of R. grahamii CCGE502 symbiotic plasmid and megaplasmid were observed in other more distant Rhizobium species. The set of conserved genes in R. grahamii comprises some of those that are highly expressed in R. phaseoli on plant roots, suggesting that they play an important role in root colonization.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32348
Martinez Romero, Esperanza; Mora, Jaime; Rogel, Marco Antonio; Torres Tejerizo, Gonzalo Arturo; Lozano, Luis; Ormeño Orrillo, Ernesto; et al.; Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia; BioMed Central; Bmc Microbiology; 14; 6; 1-2014; 1-10
1471-2180
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32348
identifier_str_mv Martinez Romero, Esperanza; Mora, Jaime; Rogel, Marco Antonio; Torres Tejerizo, Gonzalo Arturo; Lozano, Luis; Ormeño Orrillo, Ernesto; et al.; Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia; BioMed Central; Bmc Microbiology; 14; 6; 1-2014; 1-10
1471-2180
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-6
info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-14-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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