Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background

Autores
Torres Tejerizo, Gonzalo Arturo; Pistorio, Mariano; Althabegoiti, Maria Julia; Cervantes, Laura; Wibberg, Daniel; Schlüter, Andreas; Pühler, Alfred; Lagares, Antonio; Romero, David; Brom, Susana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plasmids have played a major role in bacterial evolution, mainly by theircapacity to perform horizontal gene transfer (HGT). Their conjugative transfer(CT) properties are usually described in terms of the plasmid itself. In thiswork, we analyzed structural and functional aspects of the CT of pLPU83a, anaccessory replicon fromRhizobiumsp. LPU83, able to transfer from its parentalstrain, fromEnsifer meliloti, or fromRhizobium etli. pLPU83a contains a com-plete set of transfer genes, featuring a particular organization, shared with onlytwo other rhizobial plasmids. These plasmids contain a TraR quorum-sensing(QS) transcriptional regulator, but lack an acyl-homoserine lactone (AHL) syn-thase gene. We also determined that the ability of pLPU83a to transfer fromR. etliCFN42 genomic background was mainly achieved through mobilization,employing the machinery of the endogenous plasmid pRetCFN42a, fallingunder control of the QS regulators from pRetCFN42a. In contrast, from itsnative or from theE. melilotibackground, pLPU83a utilized its own machineryfor conjugation, requiring the plasmid-encodedtraR.Activation of TraRseemed to be AHL independent. The results obtained indicate that the CT phe-notype of a plasmid is dictated not only by the genes it carries, but by theirinteraction with its genomic context.
Fil: Torres Tejerizo, Gonzalo Arturo. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Althabegoiti, Maria Julia. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Cervantes, Laura. Universidad Nacional Autónoma de México; México
Fil: Wibberg, Daniel. Universitat Bielefeld; Alemania
Fil: Schlüter, Andreas. Universitat Bielefeld; Alemania
Fil: Pühler, Alfred. Universitat Bielefeld; Alemania
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Romero, David. Universidad Nacional Autónoma de México; México
Fil: Brom, Susana. Universidad Nacional Autónoma de México; México
Materia
rhizobia
plasmid
conjugative transfer regulation
luxR regulators
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32383
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/32383
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic backgroundTorres Tejerizo, Gonzalo ArturoPistorio, MarianoAlthabegoiti, Maria JuliaCervantes, LauraWibberg, DanielSchlüter, AndreasPühler, AlfredLagares, AntonioRomero, DavidBrom, Susanarhizobiaplasmidconjugative transfer regulationluxR regulatorshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plasmids have played a major role in bacterial evolution, mainly by theircapacity to perform horizontal gene transfer (HGT). Their conjugative transfer(CT) properties are usually described in terms of the plasmid itself. In thiswork, we analyzed structural and functional aspects of the CT of pLPU83a, anaccessory replicon fromRhizobiumsp. LPU83, able to transfer from its parentalstrain, fromEnsifer meliloti, or fromRhizobium etli. pLPU83a contains a com-plete set of transfer genes, featuring a particular organization, shared with onlytwo other rhizobial plasmids. These plasmids contain a TraR quorum-sensing(QS) transcriptional regulator, but lack an acyl-homoserine lactone (AHL) syn-thase gene. We also determined that the ability of pLPU83a to transfer fromR. etliCFN42 genomic background was mainly achieved through mobilization,employing the machinery of the endogenous plasmid pRetCFN42a, fallingunder control of the QS regulators from pRetCFN42a. In contrast, from itsnative or from theE. melilotibackground, pLPU83a utilized its own machineryfor conjugation, requiring the plasmid-encodedtraR.Activation of TraRseemed to be AHL independent. The results obtained indicate that the CT phe-notype of a plasmid is dictated not only by the genes it carries, but by theirinteraction with its genomic context.Fil: Torres Tejerizo, Gonzalo Arturo. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Althabegoiti, Maria Julia. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Cervantes, Laura. Universidad Nacional Autónoma de México; MéxicoFil: Wibberg, Daniel. Universitat Bielefeld; AlemaniaFil: Schlüter, Andreas. Universitat Bielefeld; AlemaniaFil: Pühler, Alfred. Universitat Bielefeld; AlemaniaFil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Romero, David. Universidad Nacional Autónoma de México; MéxicoFil: Brom, Susana. Universidad Nacional Autónoma de México; MéxicoWiley Blackwell Publishing, Inc2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32383Brom, Susana; Romero, David; Lagares, Antonio; Pühler, Alfred; Schlüter, Andreas; Wibberg, Daniel; et al.; Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 88; 3; 5-2014; 565-5780168-6496CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6941.12325info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/88/3/565/586606info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:54:02Zoai:ri.conicet.gov.ar:11336/32383instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:54:02.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
spellingShingle Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
Torres Tejerizo, Gonzalo Arturo
rhizobia
plasmid
conjugative transfer regulation
luxR regulators
title_short Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_full Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_fullStr Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_full_unstemmed Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
title_sort Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background
dc.creator.none.fl_str_mv Torres Tejerizo, Gonzalo Arturo
Pistorio, Mariano
Althabegoiti, Maria Julia
Cervantes, Laura
Wibberg, Daniel
Schlüter, Andreas
Pühler, Alfred
Lagares, Antonio
Romero, David
Brom, Susana
author Torres Tejerizo, Gonzalo Arturo
author_facet Torres Tejerizo, Gonzalo Arturo
Pistorio, Mariano
Althabegoiti, Maria Julia
Cervantes, Laura
Wibberg, Daniel
Schlüter, Andreas
Pühler, Alfred
Lagares, Antonio
Romero, David
Brom, Susana
author_role author
author2 Pistorio, Mariano
Althabegoiti, Maria Julia
Cervantes, Laura
Wibberg, Daniel
Schlüter, Andreas
Pühler, Alfred
Lagares, Antonio
Romero, David
Brom, Susana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv rhizobia
plasmid
conjugative transfer regulation
luxR regulators
topic rhizobia
plasmid
conjugative transfer regulation
luxR regulators
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plasmids have played a major role in bacterial evolution, mainly by theircapacity to perform horizontal gene transfer (HGT). Their conjugative transfer(CT) properties are usually described in terms of the plasmid itself. In thiswork, we analyzed structural and functional aspects of the CT of pLPU83a, anaccessory replicon fromRhizobiumsp. LPU83, able to transfer from its parentalstrain, fromEnsifer meliloti, or fromRhizobium etli. pLPU83a contains a com-plete set of transfer genes, featuring a particular organization, shared with onlytwo other rhizobial plasmids. These plasmids contain a TraR quorum-sensing(QS) transcriptional regulator, but lack an acyl-homoserine lactone (AHL) syn-thase gene. We also determined that the ability of pLPU83a to transfer fromR. etliCFN42 genomic background was mainly achieved through mobilization,employing the machinery of the endogenous plasmid pRetCFN42a, fallingunder control of the QS regulators from pRetCFN42a. In contrast, from itsnative or from theE. melilotibackground, pLPU83a utilized its own machineryfor conjugation, requiring the plasmid-encodedtraR.Activation of TraRseemed to be AHL independent. The results obtained indicate that the CT phe-notype of a plasmid is dictated not only by the genes it carries, but by theirinteraction with its genomic context.
Fil: Torres Tejerizo, Gonzalo Arturo. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Althabegoiti, Maria Julia. Universidad Nacional Autónoma de México; México. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Cervantes, Laura. Universidad Nacional Autónoma de México; México
Fil: Wibberg, Daniel. Universitat Bielefeld; Alemania
Fil: Schlüter, Andreas. Universitat Bielefeld; Alemania
Fil: Pühler, Alfred. Universitat Bielefeld; Alemania
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Romero, David. Universidad Nacional Autónoma de México; México
Fil: Brom, Susana. Universidad Nacional Autónoma de México; México
description Plasmids have played a major role in bacterial evolution, mainly by theircapacity to perform horizontal gene transfer (HGT). Their conjugative transfer(CT) properties are usually described in terms of the plasmid itself. In thiswork, we analyzed structural and functional aspects of the CT of pLPU83a, anaccessory replicon fromRhizobiumsp. LPU83, able to transfer from its parentalstrain, fromEnsifer meliloti, or fromRhizobium etli. pLPU83a contains a com-plete set of transfer genes, featuring a particular organization, shared with onlytwo other rhizobial plasmids. These plasmids contain a TraR quorum-sensing(QS) transcriptional regulator, but lack an acyl-homoserine lactone (AHL) syn-thase gene. We also determined that the ability of pLPU83a to transfer fromR. etliCFN42 genomic background was mainly achieved through mobilization,employing the machinery of the endogenous plasmid pRetCFN42a, fallingunder control of the QS regulators from pRetCFN42a. In contrast, from itsnative or from theE. melilotibackground, pLPU83a utilized its own machineryfor conjugation, requiring the plasmid-encodedtraR.Activation of TraRseemed to be AHL independent. The results obtained indicate that the CT phe-notype of a plasmid is dictated not only by the genes it carries, but by theirinteraction with its genomic context.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32383
Brom, Susana; Romero, David; Lagares, Antonio; Pühler, Alfred; Schlüter, Andreas; Wibberg, Daniel; et al.; Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 88; 3; 5-2014; 565-578
0168-6496
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32383
identifier_str_mv Brom, Susana; Romero, David; Lagares, Antonio; Pühler, Alfred; Schlüter, Andreas; Wibberg, Daniel; et al.; Rhizobial plasmid pLPU83a is able to switch between different transfer machineries depending on its genomic background; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 88; 3; 5-2014; 565-578
0168-6496
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/1574-6941.12325
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/88/3/565/586606
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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