Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation

Autores
Martínez León, Eduardo Antonio; Osycka Salut, Claudia Elena; Signorelli, Janetti; Kong, Milene; Morales, Patricio; Perez Martinez, Silvina Laura; Díaz, Emilce Silvina
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fibronectin (Fn) enhances human sperm capacitation via the cAMP/PKA pathway, and the endocannabinoid system participates in this process. Moreover, Fn has been linked to endocannabinoid system components in different cellular models, even though no evidence of such interactions in human sperm is available. Normal semen samples were evaluated over a 4-year period. Our findings suggest that (a) the capacitating effects of Fn were reversed by preincubating the sperm with a cannabinoid receptor 1 (CB1) or transient receptor potential cation channel subfamily V member 1 (TRPV1) antagonist (p < 0.001 and p < 0.05, respectively); (b) cooperation between CB1 and TRPV1 may exist (p < 0.01); (c) the activity of specific fatty acid amide hydroxylase (FAAH) decreased after 1 min (p < 0.01) and increased after 60 min (p < 0.01) of capacitation in the presence of Fn; (d) the effects of Fn on FAAH activity were prevented by preincubating spermatozoa with a protein kinase A (PKA) inhibitor (p < 0.01); (e) Fn modulated both the cyclic adenosine monophosphate concentration and PKA activity (p < 0.05) during early capacitation; and (f) FAAH was a PKA substrate modulated by phosphorylation. These findings indicate that Fn stimulates human sperm capacitation via the cAMP/PKA pathway through modulation of the endocannabinoid system. Understanding the functional competence of human spermatozoa is essential for facilitating clinical advances in infertility treatment and for developing novel contraceptive strategies.
Fil: Martínez León, Eduardo Antonio. Universidad de Antofagasta; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Osycka Salut, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Signorelli, Janetti. Universidad de Antofagasta; Chile
Fil: Kong, Milene. Universidad de Antofagasta; Chile
Fil: Morales, Patricio. Universidad de Antofagasta; Chile
Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Díaz, Emilce Silvina. Universidad de Antofagasta; Chile
Materia
CAMP/PKA PATHWAY
ENDOCANNABINOID SYSTEM
FIBRONECTIN
HUMAN SPERM CAPACITATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96702
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96702
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitationMartínez León, Eduardo AntonioOsycka Salut, Claudia ElenaSignorelli, JanettiKong, MileneMorales, PatricioPerez Martinez, Silvina LauraDíaz, Emilce SilvinaCAMP/PKA PATHWAYENDOCANNABINOID SYSTEMFIBRONECTINHUMAN SPERM CAPACITATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fibronectin (Fn) enhances human sperm capacitation via the cAMP/PKA pathway, and the endocannabinoid system participates in this process. Moreover, Fn has been linked to endocannabinoid system components in different cellular models, even though no evidence of such interactions in human sperm is available. Normal semen samples were evaluated over a 4-year period. Our findings suggest that (a) the capacitating effects of Fn were reversed by preincubating the sperm with a cannabinoid receptor 1 (CB1) or transient receptor potential cation channel subfamily V member 1 (TRPV1) antagonist (p < 0.001 and p < 0.05, respectively); (b) cooperation between CB1 and TRPV1 may exist (p < 0.01); (c) the activity of specific fatty acid amide hydroxylase (FAAH) decreased after 1 min (p < 0.01) and increased after 60 min (p < 0.01) of capacitation in the presence of Fn; (d) the effects of Fn on FAAH activity were prevented by preincubating spermatozoa with a protein kinase A (PKA) inhibitor (p < 0.01); (e) Fn modulated both the cyclic adenosine monophosphate concentration and PKA activity (p < 0.05) during early capacitation; and (f) FAAH was a PKA substrate modulated by phosphorylation. These findings indicate that Fn stimulates human sperm capacitation via the cAMP/PKA pathway through modulation of the endocannabinoid system. Understanding the functional competence of human spermatozoa is essential for facilitating clinical advances in infertility treatment and for developing novel contraceptive strategies.Fil: Martínez León, Eduardo Antonio. Universidad de Antofagasta; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Osycka Salut, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Signorelli, Janetti. Universidad de Antofagasta; ChileFil: Kong, Milene. Universidad de Antofagasta; ChileFil: Morales, Patricio. Universidad de Antofagasta; ChileFil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Díaz, Emilce Silvina. Universidad de Antofagasta; ChileWiley-liss, Div John Wiley & Sons Inc2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96702Martínez León, Eduardo Antonio; Osycka Salut, Claudia Elena; Signorelli, Janetti; Kong, Milene; Morales, Patricio; et al.; Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction and Development; 86; 2; 2-2019; 224-2381040-452XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1002/mrd.23097info:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.23097info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:23Zoai:ri.conicet.gov.ar:11336/96702instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:24.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
title Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
spellingShingle Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
Martínez León, Eduardo Antonio
CAMP/PKA PATHWAY
ENDOCANNABINOID SYSTEM
FIBRONECTIN
HUMAN SPERM CAPACITATION
title_short Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
title_full Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
title_fullStr Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
title_full_unstemmed Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
title_sort Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation
dc.creator.none.fl_str_mv Martínez León, Eduardo Antonio
Osycka Salut, Claudia Elena
Signorelli, Janetti
Kong, Milene
Morales, Patricio
Perez Martinez, Silvina Laura
Díaz, Emilce Silvina
author Martínez León, Eduardo Antonio
author_facet Martínez León, Eduardo Antonio
Osycka Salut, Claudia Elena
Signorelli, Janetti
Kong, Milene
Morales, Patricio
Perez Martinez, Silvina Laura
Díaz, Emilce Silvina
author_role author
author2 Osycka Salut, Claudia Elena
Signorelli, Janetti
Kong, Milene
Morales, Patricio
Perez Martinez, Silvina Laura
Díaz, Emilce Silvina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CAMP/PKA PATHWAY
ENDOCANNABINOID SYSTEM
FIBRONECTIN
HUMAN SPERM CAPACITATION
topic CAMP/PKA PATHWAY
ENDOCANNABINOID SYSTEM
FIBRONECTIN
HUMAN SPERM CAPACITATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fibronectin (Fn) enhances human sperm capacitation via the cAMP/PKA pathway, and the endocannabinoid system participates in this process. Moreover, Fn has been linked to endocannabinoid system components in different cellular models, even though no evidence of such interactions in human sperm is available. Normal semen samples were evaluated over a 4-year period. Our findings suggest that (a) the capacitating effects of Fn were reversed by preincubating the sperm with a cannabinoid receptor 1 (CB1) or transient receptor potential cation channel subfamily V member 1 (TRPV1) antagonist (p < 0.001 and p < 0.05, respectively); (b) cooperation between CB1 and TRPV1 may exist (p < 0.01); (c) the activity of specific fatty acid amide hydroxylase (FAAH) decreased after 1 min (p < 0.01) and increased after 60 min (p < 0.01) of capacitation in the presence of Fn; (d) the effects of Fn on FAAH activity were prevented by preincubating spermatozoa with a protein kinase A (PKA) inhibitor (p < 0.01); (e) Fn modulated both the cyclic adenosine monophosphate concentration and PKA activity (p < 0.05) during early capacitation; and (f) FAAH was a PKA substrate modulated by phosphorylation. These findings indicate that Fn stimulates human sperm capacitation via the cAMP/PKA pathway through modulation of the endocannabinoid system. Understanding the functional competence of human spermatozoa is essential for facilitating clinical advances in infertility treatment and for developing novel contraceptive strategies.
Fil: Martínez León, Eduardo Antonio. Universidad de Antofagasta; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Osycka Salut, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Signorelli, Janetti. Universidad de Antofagasta; Chile
Fil: Kong, Milene. Universidad de Antofagasta; Chile
Fil: Morales, Patricio. Universidad de Antofagasta; Chile
Fil: Perez Martinez, Silvina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Díaz, Emilce Silvina. Universidad de Antofagasta; Chile
description Fibronectin (Fn) enhances human sperm capacitation via the cAMP/PKA pathway, and the endocannabinoid system participates in this process. Moreover, Fn has been linked to endocannabinoid system components in different cellular models, even though no evidence of such interactions in human sperm is available. Normal semen samples were evaluated over a 4-year period. Our findings suggest that (a) the capacitating effects of Fn were reversed by preincubating the sperm with a cannabinoid receptor 1 (CB1) or transient receptor potential cation channel subfamily V member 1 (TRPV1) antagonist (p < 0.001 and p < 0.05, respectively); (b) cooperation between CB1 and TRPV1 may exist (p < 0.01); (c) the activity of specific fatty acid amide hydroxylase (FAAH) decreased after 1 min (p < 0.01) and increased after 60 min (p < 0.01) of capacitation in the presence of Fn; (d) the effects of Fn on FAAH activity were prevented by preincubating spermatozoa with a protein kinase A (PKA) inhibitor (p < 0.01); (e) Fn modulated both the cyclic adenosine monophosphate concentration and PKA activity (p < 0.05) during early capacitation; and (f) FAAH was a PKA substrate modulated by phosphorylation. These findings indicate that Fn stimulates human sperm capacitation via the cAMP/PKA pathway through modulation of the endocannabinoid system. Understanding the functional competence of human spermatozoa is essential for facilitating clinical advances in infertility treatment and for developing novel contraceptive strategies.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96702
Martínez León, Eduardo Antonio; Osycka Salut, Claudia Elena; Signorelli, Janetti; Kong, Milene; Morales, Patricio; et al.; Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction and Development; 86; 2; 2-2019; 224-238
1040-452X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96702
identifier_str_mv Martínez León, Eduardo Antonio; Osycka Salut, Claudia Elena; Signorelli, Janetti; Kong, Milene; Morales, Patricio; et al.; Fibronectin modulates the endocannabinoid system through the cAMP/PKA pathway during human sperm capacitation; Wiley-liss, Div John Wiley & Sons Inc; Molecular Reproduction and Development; 86; 2; 2-2019; 224-238
1040-452X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://doi.wiley.com/10.1002/mrd.23097
info:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.23097
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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