Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary

Autores
Godoi, Filipe G. Andrade; Forner Piquer, Isabel; Randazzo, Basilio; Habibi, Hamid R.; Lo Nostro, Fabiana Laura; Moreira, Renata Guimarães; Carnevali, Oliana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.
Fil: Godoi, Filipe G. Andrade. Università Politecnica dele Marche; Italia. Universidade de Sao Paulo; Brasil
Fil: Forner Piquer, Isabel. Università Politecnica dele Marche; Italia
Fil: Randazzo, Basilio. Università Politecnica dele Marche; Italia
Fil: Habibi, Hamid R.. University of Calgary; Canadá
Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Moreira, Renata Guimarães. Universidade de Sao Paulo; Brasil
Fil: Carnevali, Oliana. Università Politecnica dele Marche; Italia. Consorzio Interuniversitario di Biosistemi e Biostrutture; Italia
Materia
AQUATIC TOXICOLOGY
DANIO RERIO
ENDOCRINE DISRUPTION
FOLLICULAR ATRESIA
OXIDATIVE STRESS
PHTHALATE
REPRODUCTIVE BIOMARKERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181577
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181577
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish OvaryGodoi, Filipe G. AndradeForner Piquer, IsabelRandazzo, BasilioHabibi, Hamid R.Lo Nostro, Fabiana LauraMoreira, Renata GuimarãesCarnevali, OlianaAQUATIC TOXICOLOGYDANIO RERIOENDOCRINE DISRUPTIONFOLLICULAR ATRESIAOXIDATIVE STRESSPHTHALATEREPRODUCTIVE BIOMARKERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.Fil: Godoi, Filipe G. Andrade. Università Politecnica dele Marche; Italia. Universidade de Sao Paulo; BrasilFil: Forner Piquer, Isabel. Università Politecnica dele Marche; ItaliaFil: Randazzo, Basilio. Università Politecnica dele Marche; ItaliaFil: Habibi, Hamid R.. University of Calgary; CanadáFil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Moreira, Renata Guimarães. Universidade de Sao Paulo; BrasilFil: Carnevali, Oliana. Università Politecnica dele Marche; Italia. Consorzio Interuniversitario di Biosistemi e Biostrutture; ItaliaFrontiers Media2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181577Godoi, Filipe G. Andrade; Forner Piquer, Isabel; Randazzo, Basilio; Habibi, Hamid R.; Lo Nostro, Fabiana Laura; et al.; Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary; Frontiers Media; Frontiers in Endocrinology; 12; 6-2021; 1-101664-2392CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fendo.2021.677853/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2021.677853info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:09:42Zoai:ri.conicet.gov.ar:11336/181577instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:09:43.205CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
title Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
spellingShingle Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
Godoi, Filipe G. Andrade
AQUATIC TOXICOLOGY
DANIO RERIO
ENDOCRINE DISRUPTION
FOLLICULAR ATRESIA
OXIDATIVE STRESS
PHTHALATE
REPRODUCTIVE BIOMARKERS
title_short Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
title_full Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
title_fullStr Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
title_full_unstemmed Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
title_sort Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary
dc.creator.none.fl_str_mv Godoi, Filipe G. Andrade
Forner Piquer, Isabel
Randazzo, Basilio
Habibi, Hamid R.
Lo Nostro, Fabiana Laura
Moreira, Renata Guimarães
Carnevali, Oliana
author Godoi, Filipe G. Andrade
author_facet Godoi, Filipe G. Andrade
Forner Piquer, Isabel
Randazzo, Basilio
Habibi, Hamid R.
Lo Nostro, Fabiana Laura
Moreira, Renata Guimarães
Carnevali, Oliana
author_role author
author2 Forner Piquer, Isabel
Randazzo, Basilio
Habibi, Hamid R.
Lo Nostro, Fabiana Laura
Moreira, Renata Guimarães
Carnevali, Oliana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AQUATIC TOXICOLOGY
DANIO RERIO
ENDOCRINE DISRUPTION
FOLLICULAR ATRESIA
OXIDATIVE STRESS
PHTHALATE
REPRODUCTIVE BIOMARKERS
topic AQUATIC TOXICOLOGY
DANIO RERIO
ENDOCRINE DISRUPTION
FOLLICULAR ATRESIA
OXIDATIVE STRESS
PHTHALATE
REPRODUCTIVE BIOMARKERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.
Fil: Godoi, Filipe G. Andrade. Università Politecnica dele Marche; Italia. Universidade de Sao Paulo; Brasil
Fil: Forner Piquer, Isabel. Università Politecnica dele Marche; Italia
Fil: Randazzo, Basilio. Università Politecnica dele Marche; Italia
Fil: Habibi, Hamid R.. University of Calgary; Canadá
Fil: Lo Nostro, Fabiana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Moreira, Renata Guimarães. Universidade de Sao Paulo; Brasil
Fil: Carnevali, Oliana. Università Politecnica dele Marche; Italia. Consorzio Interuniversitario di Biosistemi e Biostrutture; Italia
description Di-isononyl phthalate (DiNP) is a plasticizer reported to elicit hormone-like activity and disrupt metabolism and reproduction in fish and other vertebrates. In general, phthalates have been used at high concentrations beyond reported environmental levels to assess their adverse effects on fish gonadal physiology. The present study exposed adult female zebrafish to a wide range of DiNP concentrations [0.42 µg L−1 (10−9 M), 4.2 µg L−1 (10−8 M), and 42 µg L−1 (10−7 M)] for 21 days. We evaluated gene expression profiles related to apoptosis, autophagy, and oxidative stress; DNA fragmentation (TUNEL assay: terminal deoxynucleotidyl transferase dUTP nick end labeling) and caspase activity (CAS3) were also examined. Exposure to 0.42 and 4.2 µg L−1 upregulated the genes coding for tnfa and baxa, sod1, prkaa1, respectively. CAS3 immunohistochemistry revealed a higher number of positive vitellogenic oocytes in ovaries exposed to 0.42 µg L−1. Subsequently, we examined the relationship between CAS3 signaling and DNA fragmentation. Accordingly, DNA fragmentation was observed in vitellogenic follicles of fish exposed to 0.42 and 4.2 μg L−1. Our results demonstrate that follicular atresia can occur after exposure to environmental levels of DiNP for 21 days, which may adversely affect the reproductive performance of female zebrafish in a non-monotonic manner.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181577
Godoi, Filipe G. Andrade; Forner Piquer, Isabel; Randazzo, Basilio; Habibi, Hamid R.; Lo Nostro, Fabiana Laura; et al.; Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary; Frontiers Media; Frontiers in Endocrinology; 12; 6-2021; 1-10
1664-2392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181577
identifier_str_mv Godoi, Filipe G. Andrade; Forner Piquer, Isabel; Randazzo, Basilio; Habibi, Hamid R.; Lo Nostro, Fabiana Laura; et al.; Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary; Frontiers Media; Frontiers in Endocrinology; 12; 6-2021; 1-10
1664-2392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fendo.2021.677853/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2021.677853
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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