Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes

Autores
Ramos, Maria Victoria; Fernández, Gabriela Cristina; Fernández Brando, Romina Jimena; Panek, Cecilia Analía; Bentancor, Leticia Verónica; Landoni, Verónica Inés; Isturiz, Martín Amadeo; Palermo, Marina Sandra
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The membrane-anchored form of the chemokine fractalkine (CX3CL1) has been identified as a novel adhesion molecule that interacts with its specific receptor (CX3CR1) expressed in monocytes, T cells and natural killer cells to induce adhesion. In addition, CX3CL1 can be cleaved from the cell membrane to induce chemotaxis of CX3CR1- expressing leucocytes. Recently, marked variations in CX3CR1 monocyte expression have been observed during several pathological conditions. Regulation of CX3CR1 in monocytes during basal or inflammatory/anti-inflammatory conditions is poorly understood. The aim of this study was therefore to examine CX3CR1 expression during monocyte maturation and the effect of soluble mediators on this process. We found that basal expression of CX3CR1 in fresh monocytes was reduced during culture, and that lipopolysacchairde accelerated this effect. In contrast, interleukin-10 and interferon-γ treatment abrogated CX3CR1 down-modulation, through a phosphatidylinositol 3 kinase-dependent pathway. Most importantly, CX3CR1 membrane expression correlated with monocyte CX3CL1-dependent function. Taken together, our data demonstrate that CX3CR1 expression in monocytes can be modulated, and suggest that alterations in their environment are able to influence CX3CL1- dependent functions, such as chemotaxis and adhesion, leading to changes in the kinetics, composition and/or functional status of the leucocyte infiltrate. © 2010 The Authors.
Fil: Ramos, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fernández, Gabriela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Panek, Cecilia Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Bentancor, Leticia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Landoni, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Materia
Chemokine Receptors
Cytokines
Human Macrophages/Monocytes
Innate Immunity
Signalling/Signal Transduction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53000
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytesRamos, Maria VictoriaFernández, Gabriela CristinaFernández Brando, Romina JimenaPanek, Cecilia AnalíaBentancor, Leticia VerónicaLandoni, Verónica InésIsturiz, Martín AmadeoPalermo, Marina SandraChemokine ReceptorsCytokinesHuman Macrophages/MonocytesInnate ImmunitySignalling/Signal Transductionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The membrane-anchored form of the chemokine fractalkine (CX3CL1) has been identified as a novel adhesion molecule that interacts with its specific receptor (CX3CR1) expressed in monocytes, T cells and natural killer cells to induce adhesion. In addition, CX3CL1 can be cleaved from the cell membrane to induce chemotaxis of CX3CR1- expressing leucocytes. Recently, marked variations in CX3CR1 monocyte expression have been observed during several pathological conditions. Regulation of CX3CR1 in monocytes during basal or inflammatory/anti-inflammatory conditions is poorly understood. The aim of this study was therefore to examine CX3CR1 expression during monocyte maturation and the effect of soluble mediators on this process. We found that basal expression of CX3CR1 in fresh monocytes was reduced during culture, and that lipopolysacchairde accelerated this effect. In contrast, interleukin-10 and interferon-γ treatment abrogated CX3CR1 down-modulation, through a phosphatidylinositol 3 kinase-dependent pathway. Most importantly, CX3CR1 membrane expression correlated with monocyte CX3CL1-dependent function. Taken together, our data demonstrate that CX3CR1 expression in monocytes can be modulated, and suggest that alterations in their environment are able to influence CX3CL1- dependent functions, such as chemotaxis and adhesion, leading to changes in the kinetics, composition and/or functional status of the leucocyte infiltrate. © 2010 The Authors.Fil: Ramos, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Fernández, Gabriela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; ArgentinaFil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; ArgentinaFil: Panek, Cecilia Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; ArgentinaFil: Bentancor, Leticia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Landoni, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; ArgentinaFil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; ArgentinaFil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; ArgentinaWiley Blackwell Publishing, Inc2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53000Ramos, Maria Victoria; Fernández, Gabriela Cristina; Fernández Brando, Romina Jimena; Panek, Cecilia Analía; Bentancor, Leticia Verónica; et al.; Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes; Wiley Blackwell Publishing, Inc; Immunology; 129; 4; 4-2010; 600-6090019-2805CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2567.2009.03181.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2567.2009.03181.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:24:59Zoai:ri.conicet.gov.ar:11336/53000instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:24:59.819CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
title Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
spellingShingle Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
Ramos, Maria Victoria
Chemokine Receptors
Cytokines
Human Macrophages/Monocytes
Innate Immunity
Signalling/Signal Transduction
title_short Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
title_full Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
title_fullStr Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
title_full_unstemmed Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
title_sort Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes
dc.creator.none.fl_str_mv Ramos, Maria Victoria
Fernández, Gabriela Cristina
Fernández Brando, Romina Jimena
Panek, Cecilia Analía
Bentancor, Leticia Verónica
Landoni, Verónica Inés
Isturiz, Martín Amadeo
Palermo, Marina Sandra
author Ramos, Maria Victoria
author_facet Ramos, Maria Victoria
Fernández, Gabriela Cristina
Fernández Brando, Romina Jimena
Panek, Cecilia Analía
Bentancor, Leticia Verónica
Landoni, Verónica Inés
Isturiz, Martín Amadeo
Palermo, Marina Sandra
author_role author
author2 Fernández, Gabriela Cristina
Fernández Brando, Romina Jimena
Panek, Cecilia Analía
Bentancor, Leticia Verónica
Landoni, Verónica Inés
Isturiz, Martín Amadeo
Palermo, Marina Sandra
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chemokine Receptors
Cytokines
Human Macrophages/Monocytes
Innate Immunity
Signalling/Signal Transduction
topic Chemokine Receptors
Cytokines
Human Macrophages/Monocytes
Innate Immunity
Signalling/Signal Transduction
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The membrane-anchored form of the chemokine fractalkine (CX3CL1) has been identified as a novel adhesion molecule that interacts with its specific receptor (CX3CR1) expressed in monocytes, T cells and natural killer cells to induce adhesion. In addition, CX3CL1 can be cleaved from the cell membrane to induce chemotaxis of CX3CR1- expressing leucocytes. Recently, marked variations in CX3CR1 monocyte expression have been observed during several pathological conditions. Regulation of CX3CR1 in monocytes during basal or inflammatory/anti-inflammatory conditions is poorly understood. The aim of this study was therefore to examine CX3CR1 expression during monocyte maturation and the effect of soluble mediators on this process. We found that basal expression of CX3CR1 in fresh monocytes was reduced during culture, and that lipopolysacchairde accelerated this effect. In contrast, interleukin-10 and interferon-γ treatment abrogated CX3CR1 down-modulation, through a phosphatidylinositol 3 kinase-dependent pathway. Most importantly, CX3CR1 membrane expression correlated with monocyte CX3CL1-dependent function. Taken together, our data demonstrate that CX3CR1 expression in monocytes can be modulated, and suggest that alterations in their environment are able to influence CX3CL1- dependent functions, such as chemotaxis and adhesion, leading to changes in the kinetics, composition and/or functional status of the leucocyte infiltrate. © 2010 The Authors.
Fil: Ramos, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fernández, Gabriela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Panek, Cecilia Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Bentancor, Leticia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Landoni, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Leucemia Experimental; Argentina
description The membrane-anchored form of the chemokine fractalkine (CX3CL1) has been identified as a novel adhesion molecule that interacts with its specific receptor (CX3CR1) expressed in monocytes, T cells and natural killer cells to induce adhesion. In addition, CX3CL1 can be cleaved from the cell membrane to induce chemotaxis of CX3CR1- expressing leucocytes. Recently, marked variations in CX3CR1 monocyte expression have been observed during several pathological conditions. Regulation of CX3CR1 in monocytes during basal or inflammatory/anti-inflammatory conditions is poorly understood. The aim of this study was therefore to examine CX3CR1 expression during monocyte maturation and the effect of soluble mediators on this process. We found that basal expression of CX3CR1 in fresh monocytes was reduced during culture, and that lipopolysacchairde accelerated this effect. In contrast, interleukin-10 and interferon-γ treatment abrogated CX3CR1 down-modulation, through a phosphatidylinositol 3 kinase-dependent pathway. Most importantly, CX3CR1 membrane expression correlated with monocyte CX3CL1-dependent function. Taken together, our data demonstrate that CX3CR1 expression in monocytes can be modulated, and suggest that alterations in their environment are able to influence CX3CL1- dependent functions, such as chemotaxis and adhesion, leading to changes in the kinetics, composition and/or functional status of the leucocyte infiltrate. © 2010 The Authors.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53000
Ramos, Maria Victoria; Fernández, Gabriela Cristina; Fernández Brando, Romina Jimena; Panek, Cecilia Analía; Bentancor, Leticia Verónica; et al.; Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes; Wiley Blackwell Publishing, Inc; Immunology; 129; 4; 4-2010; 600-609
0019-2805
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53000
identifier_str_mv Ramos, Maria Victoria; Fernández, Gabriela Cristina; Fernández Brando, Romina Jimena; Panek, Cecilia Analía; Bentancor, Leticia Verónica; et al.; Interleukin-10 and interferon-γ modulate surface expression of fractalkine-receptor (CX3CR1) via PI3K in monocytes; Wiley Blackwell Publishing, Inc; Immunology; 129; 4; 4-2010; 600-609
0019-2805
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2567.2009.03181.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2567.2009.03181.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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