Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes

Autores
Villasuso, Ana Laura; Aveldaño, Marta Isabel; Vicario, A.; Machado Domenech, E. E.; Garcia, Mirta Beatriz
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Physiological and cellular adaptations to environmental changes are known to be related to modifications in membrane lipids. This work provides metabolic and compositional evidence that Trypanosoma cruzi epimastigotes are able to synthesize and desaturate fatty acids, to incorporate them into their lipids, and to modify this incorporation when carbamoylcholine is present in the medium. The fatty acids formed from [2-14C]acetate in the period from 2 to 9 days were mostly (70%) incorporated in phospholipids, the remainder 30% being recovered in neutral lipids, such as triacylglycerols (TAG) and diacylglycerols (DAG). The main fatty acids formed from [2-14C]acetate were saturates (16:0, 18:0), monoenes (16:1, 18:1) and dienes (mostly 18:2). The ratios between labelled unsaturated and saturated fatty acids increased continuously with growth, consistent with a precursor–product relationship between the main fatty acids, and with the occurrence in T. cruzi of Δ9- and Δ12-desaturases. From days 2 to 5, [14C]18:2 was the main fatty acid produced. Accordingly, the fatty acid profiles showed a significant increase in the percentage of 18:2 in all lipids in the period under study, especially in the first 2 to 5 days. In the presence of carbamoylcholine, the labelling of DAG and TAG with [14C]18:2 augmented. The results indicate that T cruzi is able to synthesize the main types of fatty acids required to form its membrane lipids, and to exchange them actively in response to environmental stimuli.
Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vicario, A.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Machado Domenech, E. E.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Garcia, Mirta Beatriz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Trypanosoma Cruzi
Linoleic Acid
Carbamoylcholine
Epimastigotes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43484
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43484
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotesVillasuso, Ana LauraAveldaño, Marta IsabelVicario, A.Machado Domenech, E. E.Garcia, Mirta BeatrizTrypanosoma CruziLinoleic AcidCarbamoylcholineEpimastigoteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Physiological and cellular adaptations to environmental changes are known to be related to modifications in membrane lipids. This work provides metabolic and compositional evidence that Trypanosoma cruzi epimastigotes are able to synthesize and desaturate fatty acids, to incorporate them into their lipids, and to modify this incorporation when carbamoylcholine is present in the medium. The fatty acids formed from [2-14C]acetate in the period from 2 to 9 days were mostly (70%) incorporated in phospholipids, the remainder 30% being recovered in neutral lipids, such as triacylglycerols (TAG) and diacylglycerols (DAG). The main fatty acids formed from [2-14C]acetate were saturates (16:0, 18:0), monoenes (16:1, 18:1) and dienes (mostly 18:2). The ratios between labelled unsaturated and saturated fatty acids increased continuously with growth, consistent with a precursor–product relationship between the main fatty acids, and with the occurrence in T. cruzi of Δ9- and Δ12-desaturases. From days 2 to 5, [14C]18:2 was the main fatty acid produced. Accordingly, the fatty acid profiles showed a significant increase in the percentage of 18:2 in all lipids in the period under study, especially in the first 2 to 5 days. In the presence of carbamoylcholine, the labelling of DAG and TAG with [14C]18:2 augmented. The results indicate that T cruzi is able to synthesize the main types of fatty acids required to form its membrane lipids, and to exchange them actively in response to environmental stimuli.Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Vicario, A.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Machado Domenech, E. E.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Garcia, Mirta Beatriz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2005-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43484Villasuso, Ana Laura; Aveldaño, Marta Isabel; Vicario, A.; Machado Domenech, E. E.; Garcia, Mirta Beatriz; Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1735; 3; 8-2005; 185-1911388-1981CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2005.06.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1388198105001344info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:16Zoai:ri.conicet.gov.ar:11336/43484instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:16.311CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
title Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
spellingShingle Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
Villasuso, Ana Laura
Trypanosoma Cruzi
Linoleic Acid
Carbamoylcholine
Epimastigotes
title_short Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
title_full Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
title_fullStr Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
title_full_unstemmed Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
title_sort Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes
dc.creator.none.fl_str_mv Villasuso, Ana Laura
Aveldaño, Marta Isabel
Vicario, A.
Machado Domenech, E. E.
Garcia, Mirta Beatriz
author Villasuso, Ana Laura
author_facet Villasuso, Ana Laura
Aveldaño, Marta Isabel
Vicario, A.
Machado Domenech, E. E.
Garcia, Mirta Beatriz
author_role author
author2 Aveldaño, Marta Isabel
Vicario, A.
Machado Domenech, E. E.
Garcia, Mirta Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma Cruzi
Linoleic Acid
Carbamoylcholine
Epimastigotes
topic Trypanosoma Cruzi
Linoleic Acid
Carbamoylcholine
Epimastigotes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Physiological and cellular adaptations to environmental changes are known to be related to modifications in membrane lipids. This work provides metabolic and compositional evidence that Trypanosoma cruzi epimastigotes are able to synthesize and desaturate fatty acids, to incorporate them into their lipids, and to modify this incorporation when carbamoylcholine is present in the medium. The fatty acids formed from [2-14C]acetate in the period from 2 to 9 days were mostly (70%) incorporated in phospholipids, the remainder 30% being recovered in neutral lipids, such as triacylglycerols (TAG) and diacylglycerols (DAG). The main fatty acids formed from [2-14C]acetate were saturates (16:0, 18:0), monoenes (16:1, 18:1) and dienes (mostly 18:2). The ratios between labelled unsaturated and saturated fatty acids increased continuously with growth, consistent with a precursor–product relationship between the main fatty acids, and with the occurrence in T. cruzi of Δ9- and Δ12-desaturases. From days 2 to 5, [14C]18:2 was the main fatty acid produced. Accordingly, the fatty acid profiles showed a significant increase in the percentage of 18:2 in all lipids in the period under study, especially in the first 2 to 5 days. In the presence of carbamoylcholine, the labelling of DAG and TAG with [14C]18:2 augmented. The results indicate that T cruzi is able to synthesize the main types of fatty acids required to form its membrane lipids, and to exchange them actively in response to environmental stimuli.
Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vicario, A.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Machado Domenech, E. E.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Garcia, Mirta Beatriz. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Physiological and cellular adaptations to environmental changes are known to be related to modifications in membrane lipids. This work provides metabolic and compositional evidence that Trypanosoma cruzi epimastigotes are able to synthesize and desaturate fatty acids, to incorporate them into their lipids, and to modify this incorporation when carbamoylcholine is present in the medium. The fatty acids formed from [2-14C]acetate in the period from 2 to 9 days were mostly (70%) incorporated in phospholipids, the remainder 30% being recovered in neutral lipids, such as triacylglycerols (TAG) and diacylglycerols (DAG). The main fatty acids formed from [2-14C]acetate were saturates (16:0, 18:0), monoenes (16:1, 18:1) and dienes (mostly 18:2). The ratios between labelled unsaturated and saturated fatty acids increased continuously with growth, consistent with a precursor–product relationship between the main fatty acids, and with the occurrence in T. cruzi of Δ9- and Δ12-desaturases. From days 2 to 5, [14C]18:2 was the main fatty acid produced. Accordingly, the fatty acid profiles showed a significant increase in the percentage of 18:2 in all lipids in the period under study, especially in the first 2 to 5 days. In the presence of carbamoylcholine, the labelling of DAG and TAG with [14C]18:2 augmented. The results indicate that T cruzi is able to synthesize the main types of fatty acids required to form its membrane lipids, and to exchange them actively in response to environmental stimuli.
publishDate 2005
dc.date.none.fl_str_mv 2005-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43484
Villasuso, Ana Laura; Aveldaño, Marta Isabel; Vicario, A.; Machado Domenech, E. E.; Garcia, Mirta Beatriz; Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1735; 3; 8-2005; 185-191
1388-1981
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43484
identifier_str_mv Villasuso, Ana Laura; Aveldaño, Marta Isabel; Vicario, A.; Machado Domenech, E. E.; Garcia, Mirta Beatriz; Culture age and carbamoylcholine increase the incorporation of endogenously synthesized linoleic acid in lipids of Trypanosoma cruzi epimastigotes; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1735; 3; 8-2005; 185-191
1388-1981
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2005.06.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1388198105001344
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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