Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes

Autores
Barisón, María Julia; Rapado, Ludmila Nakamura; Merino, Emilio F.; Furusho Pral, Elizabeth Mieko; Mantilla, Brian Suarez; Marchese, Letícia; Nowicki, Cristina; Silber, Ariel Mariano; Cassera, Maria Belen
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi, the etiological agent of Chagas disease, is a protozoan parasite with a complex life cycle involving a triatomine insect and mammals. Throughout its life cycle, the T. cruzi parasite faces several alternating events of cell division and cell differentiation in which exponential and stationary growth phases play key biological roles. It is well accepted that arrest of the cell division in the epimastigote stage, both in the midgut of the triatomine insect and in vitro, is required for metacyclogenesis, and it has been previously shown that the parasites change the expression profile of several proteins when entering this quiescent stage. However, little is known about the metabolic changes that epimastigotes undergo before they develop into the metacyclic trypomastigote stage. We applied targeted metabolomics to measure the metabolic intermediates in the most relevant pathways for energy metabolism and oxidative imbalance in exponentially growing and stationary growth-arrested epimastigote parasites. We show for the first time that T. cruzi epimastigotes transitioning from the exponential to the stationary phase exhibit a finely tuned adaptive metabolic mechanism that enables switching from glucose to amino acid consumption, which is more abundant in the stationary phase. This metabolic plasticity appears to be crucial for survival of the T. cruzi parasite in the myriad different environmental conditions to which it is exposed during its life cycle.
Fil: Barisón, María Julia. Universidade de Sao Paulo; Brasil
Fil: Rapado, Ludmila Nakamura. Universidade de Sao Paulo; Brasil
Fil: Merino, Emilio F.. Virginia Tech University; Estados Unidos
Fil: Furusho Pral, Elizabeth Mieko. Universidade de Sao Paulo; Brasil
Fil: Mantilla, Brian Suarez. Universidade de Sao Paulo; Brasil
Fil: Marchese, Letícia. Universidade de Sao Paulo; Brasil
Fil: Nowicki, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Silber, Ariel Mariano. Universidade de Sao Paulo; Brasil
Fil: Cassera, Maria Belen. Virginia Tech University; Estados Unidos
Materia
Chagas disease
Trypanosoma cruzi
Cell growth
Cell metabolism
Energy metabolism
Metabolomics
Epimastigotes
Oxidative imbalance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47270
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotesBarisón, María JuliaRapado, Ludmila NakamuraMerino, Emilio F.Furusho Pral, Elizabeth MiekoMantilla, Brian SuarezMarchese, LetíciaNowicki, CristinaSilber, Ariel MarianoCassera, Maria BelenChagas diseaseTrypanosoma cruziCell growthCell metabolismEnergy metabolismMetabolomicsEpimastigotesOxidative imbalancehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi, the etiological agent of Chagas disease, is a protozoan parasite with a complex life cycle involving a triatomine insect and mammals. Throughout its life cycle, the T. cruzi parasite faces several alternating events of cell division and cell differentiation in which exponential and stationary growth phases play key biological roles. It is well accepted that arrest of the cell division in the epimastigote stage, both in the midgut of the triatomine insect and in vitro, is required for metacyclogenesis, and it has been previously shown that the parasites change the expression profile of several proteins when entering this quiescent stage. However, little is known about the metabolic changes that epimastigotes undergo before they develop into the metacyclic trypomastigote stage. We applied targeted metabolomics to measure the metabolic intermediates in the most relevant pathways for energy metabolism and oxidative imbalance in exponentially growing and stationary growth-arrested epimastigote parasites. We show for the first time that T. cruzi epimastigotes transitioning from the exponential to the stationary phase exhibit a finely tuned adaptive metabolic mechanism that enables switching from glucose to amino acid consumption, which is more abundant in the stationary phase. This metabolic plasticity appears to be crucial for survival of the T. cruzi parasite in the myriad different environmental conditions to which it is exposed during its life cycle.Fil: Barisón, María Julia. Universidade de Sao Paulo; BrasilFil: Rapado, Ludmila Nakamura. Universidade de Sao Paulo; BrasilFil: Merino, Emilio F.. Virginia Tech University; Estados UnidosFil: Furusho Pral, Elizabeth Mieko. Universidade de Sao Paulo; BrasilFil: Mantilla, Brian Suarez. Universidade de Sao Paulo; BrasilFil: Marchese, Letícia. Universidade de Sao Paulo; BrasilFil: Nowicki, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Silber, Ariel Mariano. Universidade de Sao Paulo; BrasilFil: Cassera, Maria Belen. Virginia Tech University; Estados UnidosAmerican Society for Biochemistry and Molecular Biology2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47270Barisón, María Julia; Rapado, Ludmila Nakamura; Merino, Emilio F.; Furusho Pral, Elizabeth Mieko; Mantilla, Brian Suarez; et al.; Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 292; 21; 5-2017; 8964-89770021-9258CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/292/21/8964info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M117.778522info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:57Zoai:ri.conicet.gov.ar:11336/47270instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:57.348CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
title Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
spellingShingle Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
Barisón, María Julia
Chagas disease
Trypanosoma cruzi
Cell growth
Cell metabolism
Energy metabolism
Metabolomics
Epimastigotes
Oxidative imbalance
title_short Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
title_full Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
title_fullStr Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
title_full_unstemmed Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
title_sort Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes
dc.creator.none.fl_str_mv Barisón, María Julia
Rapado, Ludmila Nakamura
Merino, Emilio F.
Furusho Pral, Elizabeth Mieko
Mantilla, Brian Suarez
Marchese, Letícia
Nowicki, Cristina
Silber, Ariel Mariano
Cassera, Maria Belen
author Barisón, María Julia
author_facet Barisón, María Julia
Rapado, Ludmila Nakamura
Merino, Emilio F.
Furusho Pral, Elizabeth Mieko
Mantilla, Brian Suarez
Marchese, Letícia
Nowicki, Cristina
Silber, Ariel Mariano
Cassera, Maria Belen
author_role author
author2 Rapado, Ludmila Nakamura
Merino, Emilio F.
Furusho Pral, Elizabeth Mieko
Mantilla, Brian Suarez
Marchese, Letícia
Nowicki, Cristina
Silber, Ariel Mariano
Cassera, Maria Belen
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chagas disease
Trypanosoma cruzi
Cell growth
Cell metabolism
Energy metabolism
Metabolomics
Epimastigotes
Oxidative imbalance
topic Chagas disease
Trypanosoma cruzi
Cell growth
Cell metabolism
Energy metabolism
Metabolomics
Epimastigotes
Oxidative imbalance
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi, the etiological agent of Chagas disease, is a protozoan parasite with a complex life cycle involving a triatomine insect and mammals. Throughout its life cycle, the T. cruzi parasite faces several alternating events of cell division and cell differentiation in which exponential and stationary growth phases play key biological roles. It is well accepted that arrest of the cell division in the epimastigote stage, both in the midgut of the triatomine insect and in vitro, is required for metacyclogenesis, and it has been previously shown that the parasites change the expression profile of several proteins when entering this quiescent stage. However, little is known about the metabolic changes that epimastigotes undergo before they develop into the metacyclic trypomastigote stage. We applied targeted metabolomics to measure the metabolic intermediates in the most relevant pathways for energy metabolism and oxidative imbalance in exponentially growing and stationary growth-arrested epimastigote parasites. We show for the first time that T. cruzi epimastigotes transitioning from the exponential to the stationary phase exhibit a finely tuned adaptive metabolic mechanism that enables switching from glucose to amino acid consumption, which is more abundant in the stationary phase. This metabolic plasticity appears to be crucial for survival of the T. cruzi parasite in the myriad different environmental conditions to which it is exposed during its life cycle.
Fil: Barisón, María Julia. Universidade de Sao Paulo; Brasil
Fil: Rapado, Ludmila Nakamura. Universidade de Sao Paulo; Brasil
Fil: Merino, Emilio F.. Virginia Tech University; Estados Unidos
Fil: Furusho Pral, Elizabeth Mieko. Universidade de Sao Paulo; Brasil
Fil: Mantilla, Brian Suarez. Universidade de Sao Paulo; Brasil
Fil: Marchese, Letícia. Universidade de Sao Paulo; Brasil
Fil: Nowicki, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Silber, Ariel Mariano. Universidade de Sao Paulo; Brasil
Fil: Cassera, Maria Belen. Virginia Tech University; Estados Unidos
description Trypanosoma cruzi, the etiological agent of Chagas disease, is a protozoan parasite with a complex life cycle involving a triatomine insect and mammals. Throughout its life cycle, the T. cruzi parasite faces several alternating events of cell division and cell differentiation in which exponential and stationary growth phases play key biological roles. It is well accepted that arrest of the cell division in the epimastigote stage, both in the midgut of the triatomine insect and in vitro, is required for metacyclogenesis, and it has been previously shown that the parasites change the expression profile of several proteins when entering this quiescent stage. However, little is known about the metabolic changes that epimastigotes undergo before they develop into the metacyclic trypomastigote stage. We applied targeted metabolomics to measure the metabolic intermediates in the most relevant pathways for energy metabolism and oxidative imbalance in exponentially growing and stationary growth-arrested epimastigote parasites. We show for the first time that T. cruzi epimastigotes transitioning from the exponential to the stationary phase exhibit a finely tuned adaptive metabolic mechanism that enables switching from glucose to amino acid consumption, which is more abundant in the stationary phase. This metabolic plasticity appears to be crucial for survival of the T. cruzi parasite in the myriad different environmental conditions to which it is exposed during its life cycle.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47270
Barisón, María Julia; Rapado, Ludmila Nakamura; Merino, Emilio F.; Furusho Pral, Elizabeth Mieko; Mantilla, Brian Suarez; et al.; Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 292; 21; 5-2017; 8964-8977
0021-9258
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47270
identifier_str_mv Barisón, María Julia; Rapado, Ludmila Nakamura; Merino, Emilio F.; Furusho Pral, Elizabeth Mieko; Mantilla, Brian Suarez; et al.; Metabolomic profiling reveals a finely tuned, starvation-induced metabolic switch in Trypanosoma cruzi epimastigotes; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 292; 21; 5-2017; 8964-8977
0021-9258
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/292/21/8964
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M117.778522
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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