Genetic Transformation of Apomictic Grasses: Progress and Constraints
- Autores
- Bellido, Andres; Souza Canadá, Eduado D.; Permingeat, Hugo Raúl; Echenique, Carmen Viviana
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The available methods for plant transformation and expansion beyond its limits remain especially critical for crop improvement. For grass species, this is even more critical, mainly due to drawbacks in in vitro regeneration. Despite the existence of many protocols in grasses to achieve genetic transformation through Agrobacterium or biolistic gene delivery, their efficiencies are genotype-dependent and still very low due to the recalcitrance of these species to in vitro regeneration. Many plant transformation facilities for cereals and other important crops may be found around the world in universities and enterprises, but this is not the case for apomictic species, many of which are C4 grasses. Moreover, apomixis (asexual reproduction by seeds) represents an additional constraint for breeding. However, the transformation of an apomictic clone is an attractive strategy, as the transgene is immediately fixed in a highly adapted genetic background, capable of large-scale clonal propagation. With the exception of some species like Brachiaria brizantha which is planted in approximately 100 M ha in Brazil, apomixis is almost non-present in economically important crops. However, as it is sometimes present in their wild relatives, the main goal is to transfer this trait to crops to fix heterosis. Until now this has been a difficult task, mainly because many aspects of apomixis are unknown. Over the last few years, many candidate genes have been identified and attempts have been made to characterize them functionally in Arabidopsis and rice. However, functional analysis in true apomictic species lags far behind, mainly due to the complexity of its genomes, of the trait itself, and the lack of efficient genetic transformation protocols. In this study, we review the current status of the in vitro culture and genetic transformation methods focusing on apomictic grasses, and the prospects for the application of new tools assayed in other related species, with two aims: to pave the way for discovering the molecular pathways involved in apomixis and to develop new capacities for breeding purposes because many of these grasses are important forage or biofuel resources.
Fil: Bellido, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Souza Canadá, Eduado D.. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Agrobiotec; Argentina
Fil: Permingeat, Hugo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Agrobiotec; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina - Materia
-
APOMICTIC GRASSES
DNA-DELIVERY METHODS
EDITING
GENETIC TRANSFORMATION
MORPHOGENIC REGULATORS
PLANT REGENERATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/149646
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Genetic Transformation of Apomictic Grasses: Progress and ConstraintsBellido, AndresSouza Canadá, Eduado D.Permingeat, Hugo RaúlEchenique, Carmen VivianaAPOMICTIC GRASSESDNA-DELIVERY METHODSEDITINGGENETIC TRANSFORMATIONMORPHOGENIC REGULATORSPLANT REGENERATIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The available methods for plant transformation and expansion beyond its limits remain especially critical for crop improvement. For grass species, this is even more critical, mainly due to drawbacks in in vitro regeneration. Despite the existence of many protocols in grasses to achieve genetic transformation through Agrobacterium or biolistic gene delivery, their efficiencies are genotype-dependent and still very low due to the recalcitrance of these species to in vitro regeneration. Many plant transformation facilities for cereals and other important crops may be found around the world in universities and enterprises, but this is not the case for apomictic species, many of which are C4 grasses. Moreover, apomixis (asexual reproduction by seeds) represents an additional constraint for breeding. However, the transformation of an apomictic clone is an attractive strategy, as the transgene is immediately fixed in a highly adapted genetic background, capable of large-scale clonal propagation. With the exception of some species like Brachiaria brizantha which is planted in approximately 100 M ha in Brazil, apomixis is almost non-present in economically important crops. However, as it is sometimes present in their wild relatives, the main goal is to transfer this trait to crops to fix heterosis. Until now this has been a difficult task, mainly because many aspects of apomixis are unknown. Over the last few years, many candidate genes have been identified and attempts have been made to characterize them functionally in Arabidopsis and rice. However, functional analysis in true apomictic species lags far behind, mainly due to the complexity of its genomes, of the trait itself, and the lack of efficient genetic transformation protocols. In this study, we review the current status of the in vitro culture and genetic transformation methods focusing on apomictic grasses, and the prospects for the application of new tools assayed in other related species, with two aims: to pave the way for discovering the molecular pathways involved in apomixis and to develop new capacities for breeding purposes because many of these grasses are important forage or biofuel resources.Fil: Bellido, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Souza Canadá, Eduado D.. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Agrobiotec; ArgentinaFil: Permingeat, Hugo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Agrobiotec; ArgentinaFil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFrontiers Media2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149646Bellido, Andres; Souza Canadá, Eduado D.; Permingeat, Hugo Raúl; Echenique, Carmen Viviana; Genetic Transformation of Apomictic Grasses: Progress and Constraints; Frontiers Media; Frontiers in Plant Science; 12; 11-2021; 1-191664-462X1664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2021.768393/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.768393info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:00Zoai:ri.conicet.gov.ar:11336/149646instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:01.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genetic Transformation of Apomictic Grasses: Progress and Constraints |
title |
Genetic Transformation of Apomictic Grasses: Progress and Constraints |
spellingShingle |
Genetic Transformation of Apomictic Grasses: Progress and Constraints Bellido, Andres APOMICTIC GRASSES DNA-DELIVERY METHODS EDITING GENETIC TRANSFORMATION MORPHOGENIC REGULATORS PLANT REGENERATION |
title_short |
Genetic Transformation of Apomictic Grasses: Progress and Constraints |
title_full |
Genetic Transformation of Apomictic Grasses: Progress and Constraints |
title_fullStr |
Genetic Transformation of Apomictic Grasses: Progress and Constraints |
title_full_unstemmed |
Genetic Transformation of Apomictic Grasses: Progress and Constraints |
title_sort |
Genetic Transformation of Apomictic Grasses: Progress and Constraints |
dc.creator.none.fl_str_mv |
Bellido, Andres Souza Canadá, Eduado D. Permingeat, Hugo Raúl Echenique, Carmen Viviana |
author |
Bellido, Andres |
author_facet |
Bellido, Andres Souza Canadá, Eduado D. Permingeat, Hugo Raúl Echenique, Carmen Viviana |
author_role |
author |
author2 |
Souza Canadá, Eduado D. Permingeat, Hugo Raúl Echenique, Carmen Viviana |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
APOMICTIC GRASSES DNA-DELIVERY METHODS EDITING GENETIC TRANSFORMATION MORPHOGENIC REGULATORS PLANT REGENERATION |
topic |
APOMICTIC GRASSES DNA-DELIVERY METHODS EDITING GENETIC TRANSFORMATION MORPHOGENIC REGULATORS PLANT REGENERATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The available methods for plant transformation and expansion beyond its limits remain especially critical for crop improvement. For grass species, this is even more critical, mainly due to drawbacks in in vitro regeneration. Despite the existence of many protocols in grasses to achieve genetic transformation through Agrobacterium or biolistic gene delivery, their efficiencies are genotype-dependent and still very low due to the recalcitrance of these species to in vitro regeneration. Many plant transformation facilities for cereals and other important crops may be found around the world in universities and enterprises, but this is not the case for apomictic species, many of which are C4 grasses. Moreover, apomixis (asexual reproduction by seeds) represents an additional constraint for breeding. However, the transformation of an apomictic clone is an attractive strategy, as the transgene is immediately fixed in a highly adapted genetic background, capable of large-scale clonal propagation. With the exception of some species like Brachiaria brizantha which is planted in approximately 100 M ha in Brazil, apomixis is almost non-present in economically important crops. However, as it is sometimes present in their wild relatives, the main goal is to transfer this trait to crops to fix heterosis. Until now this has been a difficult task, mainly because many aspects of apomixis are unknown. Over the last few years, many candidate genes have been identified and attempts have been made to characterize them functionally in Arabidopsis and rice. However, functional analysis in true apomictic species lags far behind, mainly due to the complexity of its genomes, of the trait itself, and the lack of efficient genetic transformation protocols. In this study, we review the current status of the in vitro culture and genetic transformation methods focusing on apomictic grasses, and the prospects for the application of new tools assayed in other related species, with two aims: to pave the way for discovering the molecular pathways involved in apomixis and to develop new capacities for breeding purposes because many of these grasses are important forage or biofuel resources. Fil: Bellido, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina Fil: Souza Canadá, Eduado D.. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Agrobiotec; Argentina Fil: Permingeat, Hugo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Agrobiotec; Argentina Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina |
description |
The available methods for plant transformation and expansion beyond its limits remain especially critical for crop improvement. For grass species, this is even more critical, mainly due to drawbacks in in vitro regeneration. Despite the existence of many protocols in grasses to achieve genetic transformation through Agrobacterium or biolistic gene delivery, their efficiencies are genotype-dependent and still very low due to the recalcitrance of these species to in vitro regeneration. Many plant transformation facilities for cereals and other important crops may be found around the world in universities and enterprises, but this is not the case for apomictic species, many of which are C4 grasses. Moreover, apomixis (asexual reproduction by seeds) represents an additional constraint for breeding. However, the transformation of an apomictic clone is an attractive strategy, as the transgene is immediately fixed in a highly adapted genetic background, capable of large-scale clonal propagation. With the exception of some species like Brachiaria brizantha which is planted in approximately 100 M ha in Brazil, apomixis is almost non-present in economically important crops. However, as it is sometimes present in their wild relatives, the main goal is to transfer this trait to crops to fix heterosis. Until now this has been a difficult task, mainly because many aspects of apomixis are unknown. Over the last few years, many candidate genes have been identified and attempts have been made to characterize them functionally in Arabidopsis and rice. However, functional analysis in true apomictic species lags far behind, mainly due to the complexity of its genomes, of the trait itself, and the lack of efficient genetic transformation protocols. In this study, we review the current status of the in vitro culture and genetic transformation methods focusing on apomictic grasses, and the prospects for the application of new tools assayed in other related species, with two aims: to pave the way for discovering the molecular pathways involved in apomixis and to develop new capacities for breeding purposes because many of these grasses are important forage or biofuel resources. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/149646 Bellido, Andres; Souza Canadá, Eduado D.; Permingeat, Hugo Raúl; Echenique, Carmen Viviana; Genetic Transformation of Apomictic Grasses: Progress and Constraints; Frontiers Media; Frontiers in Plant Science; 12; 11-2021; 1-19 1664-462X 1664-462X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/149646 |
identifier_str_mv |
Bellido, Andres; Souza Canadá, Eduado D.; Permingeat, Hugo Raúl; Echenique, Carmen Viviana; Genetic Transformation of Apomictic Grasses: Progress and Constraints; Frontiers Media; Frontiers in Plant Science; 12; 11-2021; 1-19 1664-462X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2021.768393/full info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.768393 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613661385031680 |
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13.070432 |