Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life

Autores
Pereira Da Costa, Javier Hernán; Rodríguez, Gustavo Rubén; Picardi, Liliana Amelia; Zorzoli, Roxana; Pratta, Guillermo Raúl
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ripening stage at harvest time determines the tomato fruit quality. After the fruit achieves its maximum size several metabolic changes of typically climacteric fruits are produced. Two cultivated genotypes of Solanum lycopersicum (Caimanta and 804627), with normal and altered fruit ripening, respectively and two accession, LA1385 of S. lycopersicum var. cerasiforme and LA722 of S. pimpinellifolium, with genes that prolong fruit shelf life, were tested to: 1) characterize and make a comparatively analysis for the transcriptome at different fruit ripening stages in genotypes that differ in fruit shelf life by cDNA-AFLP; and 2) provide further insight into the relationship between the extreme phenotypic differences for ripening among the genotypes through changes at transcriptomic level. Fruits at the breaker stage (B) were evaluated for fruit weight, firmness and fruit shelf life. The elapsed days between mature green (MG) and breaker stages Days (MG-B) as well as the elapsed days between B and red ripe (RR) stages Days (B-RR) were recorded. Comparison among ripening stages showed a great polymorphism related to the changes in gene expression. For all genotypes the transition from B to RR stages had higher polymorphism than the transition from MG to B. It was observed a great genetic variability for the phenotypic traits in agreement with the changes of gene expression. Moreover, it was observed that the transcriptome expression profiles in the initial and intermediate stages during ripening (MG and B) are more important to characterize genotypes. The wild species which have long shelf life do not show as drastic changes in gene expression as the cultivar with altered ripening that carrythe nor gene. These results suggest that the expressed or silenced genes could be involved, in some way, in the determination of the phenotypic traits evaluated in this study.
Fil: Pereira Da Costa, Javier Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Rodríguez, Gustavo Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Picardi, Liliana Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Zorzoli, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Pratta, Guillermo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
CDNA-AFLP
FRUIT RIPENING PROCESS
GENETIC VARIABILITY
NOR
SOLANUM LYCOPERSICUM
SOLANUM PIMPINELLIFOLIUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90859
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/90859
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf lifePereira Da Costa, Javier HernánRodríguez, Gustavo RubénPicardi, Liliana AmeliaZorzoli, RoxanaPratta, Guillermo RaúlCDNA-AFLPFRUIT RIPENING PROCESSGENETIC VARIABILITYNORSOLANUM LYCOPERSICUMSOLANUM PIMPINELLIFOLIUMhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The ripening stage at harvest time determines the tomato fruit quality. After the fruit achieves its maximum size several metabolic changes of typically climacteric fruits are produced. Two cultivated genotypes of Solanum lycopersicum (Caimanta and 804627), with normal and altered fruit ripening, respectively and two accession, LA1385 of S. lycopersicum var. cerasiforme and LA722 of S. pimpinellifolium, with genes that prolong fruit shelf life, were tested to: 1) characterize and make a comparatively analysis for the transcriptome at different fruit ripening stages in genotypes that differ in fruit shelf life by cDNA-AFLP; and 2) provide further insight into the relationship between the extreme phenotypic differences for ripening among the genotypes through changes at transcriptomic level. Fruits at the breaker stage (B) were evaluated for fruit weight, firmness and fruit shelf life. The elapsed days between mature green (MG) and breaker stages Days (MG-B) as well as the elapsed days between B and red ripe (RR) stages Days (B-RR) were recorded. Comparison among ripening stages showed a great polymorphism related to the changes in gene expression. For all genotypes the transition from B to RR stages had higher polymorphism than the transition from MG to B. It was observed a great genetic variability for the phenotypic traits in agreement with the changes of gene expression. Moreover, it was observed that the transcriptome expression profiles in the initial and intermediate stages during ripening (MG and B) are more important to characterize genotypes. The wild species which have long shelf life do not show as drastic changes in gene expression as the cultivar with altered ripening that carrythe nor gene. These results suggest that the expressed or silenced genes could be involved, in some way, in the determination of the phenotypic traits evaluated in this study.Fil: Pereira Da Costa, Javier Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Rodríguez, Gustavo Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Picardi, Liliana Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Zorzoli, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Pratta, Guillermo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaElsevier Science2018-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/90859Pereira Da Costa, Javier Hernán; Rodríguez, Gustavo Rubén; Picardi, Liliana Amelia; Zorzoli, Roxana; Pratta, Guillermo Raúl; Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life; Elsevier Science; Scientia Horticulturae; 229; 2-2018; 125-1310304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2017.11.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:31Zoai:ri.conicet.gov.ar:11336/90859instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:32.089CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
title Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
spellingShingle Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
Pereira Da Costa, Javier Hernán
CDNA-AFLP
FRUIT RIPENING PROCESS
GENETIC VARIABILITY
NOR
SOLANUM LYCOPERSICUM
SOLANUM PIMPINELLIFOLIUM
title_short Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
title_full Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
title_fullStr Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
title_full_unstemmed Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
title_sort Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life
dc.creator.none.fl_str_mv Pereira Da Costa, Javier Hernán
Rodríguez, Gustavo Rubén
Picardi, Liliana Amelia
Zorzoli, Roxana
Pratta, Guillermo Raúl
author Pereira Da Costa, Javier Hernán
author_facet Pereira Da Costa, Javier Hernán
Rodríguez, Gustavo Rubén
Picardi, Liliana Amelia
Zorzoli, Roxana
Pratta, Guillermo Raúl
author_role author
author2 Rodríguez, Gustavo Rubén
Picardi, Liliana Amelia
Zorzoli, Roxana
Pratta, Guillermo Raúl
author2_role author
author
author
author
dc.subject.none.fl_str_mv CDNA-AFLP
FRUIT RIPENING PROCESS
GENETIC VARIABILITY
NOR
SOLANUM LYCOPERSICUM
SOLANUM PIMPINELLIFOLIUM
topic CDNA-AFLP
FRUIT RIPENING PROCESS
GENETIC VARIABILITY
NOR
SOLANUM LYCOPERSICUM
SOLANUM PIMPINELLIFOLIUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The ripening stage at harvest time determines the tomato fruit quality. After the fruit achieves its maximum size several metabolic changes of typically climacteric fruits are produced. Two cultivated genotypes of Solanum lycopersicum (Caimanta and 804627), with normal and altered fruit ripening, respectively and two accession, LA1385 of S. lycopersicum var. cerasiforme and LA722 of S. pimpinellifolium, with genes that prolong fruit shelf life, were tested to: 1) characterize and make a comparatively analysis for the transcriptome at different fruit ripening stages in genotypes that differ in fruit shelf life by cDNA-AFLP; and 2) provide further insight into the relationship between the extreme phenotypic differences for ripening among the genotypes through changes at transcriptomic level. Fruits at the breaker stage (B) were evaluated for fruit weight, firmness and fruit shelf life. The elapsed days between mature green (MG) and breaker stages Days (MG-B) as well as the elapsed days between B and red ripe (RR) stages Days (B-RR) were recorded. Comparison among ripening stages showed a great polymorphism related to the changes in gene expression. For all genotypes the transition from B to RR stages had higher polymorphism than the transition from MG to B. It was observed a great genetic variability for the phenotypic traits in agreement with the changes of gene expression. Moreover, it was observed that the transcriptome expression profiles in the initial and intermediate stages during ripening (MG and B) are more important to characterize genotypes. The wild species which have long shelf life do not show as drastic changes in gene expression as the cultivar with altered ripening that carrythe nor gene. These results suggest that the expressed or silenced genes could be involved, in some way, in the determination of the phenotypic traits evaluated in this study.
Fil: Pereira Da Costa, Javier Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Rodríguez, Gustavo Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Picardi, Liliana Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Zorzoli, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Pratta, Guillermo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description The ripening stage at harvest time determines the tomato fruit quality. After the fruit achieves its maximum size several metabolic changes of typically climacteric fruits are produced. Two cultivated genotypes of Solanum lycopersicum (Caimanta and 804627), with normal and altered fruit ripening, respectively and two accession, LA1385 of S. lycopersicum var. cerasiforme and LA722 of S. pimpinellifolium, with genes that prolong fruit shelf life, were tested to: 1) characterize and make a comparatively analysis for the transcriptome at different fruit ripening stages in genotypes that differ in fruit shelf life by cDNA-AFLP; and 2) provide further insight into the relationship between the extreme phenotypic differences for ripening among the genotypes through changes at transcriptomic level. Fruits at the breaker stage (B) were evaluated for fruit weight, firmness and fruit shelf life. The elapsed days between mature green (MG) and breaker stages Days (MG-B) as well as the elapsed days between B and red ripe (RR) stages Days (B-RR) were recorded. Comparison among ripening stages showed a great polymorphism related to the changes in gene expression. For all genotypes the transition from B to RR stages had higher polymorphism than the transition from MG to B. It was observed a great genetic variability for the phenotypic traits in agreement with the changes of gene expression. Moreover, it was observed that the transcriptome expression profiles in the initial and intermediate stages during ripening (MG and B) are more important to characterize genotypes. The wild species which have long shelf life do not show as drastic changes in gene expression as the cultivar with altered ripening that carrythe nor gene. These results suggest that the expressed or silenced genes could be involved, in some way, in the determination of the phenotypic traits evaluated in this study.
publishDate 2018
dc.date.none.fl_str_mv 2018-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90859
Pereira Da Costa, Javier Hernán; Rodríguez, Gustavo Rubén; Picardi, Liliana Amelia; Zorzoli, Roxana; Pratta, Guillermo Raúl; Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life; Elsevier Science; Scientia Horticulturae; 229; 2-2018; 125-131
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90859
identifier_str_mv Pereira Da Costa, Javier Hernán; Rodríguez, Gustavo Rubén; Picardi, Liliana Amelia; Zorzoli, Roxana; Pratta, Guillermo Raúl; Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life; Elsevier Science; Scientia Horticulturae; 229; 2-2018; 125-131
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2017.11.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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