Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1

Autores
Volpe, Verónica; Perez, Sandra; Dolcini, Guillermina Laura; Buzzola, Fernanda Roxana; Grunhut, Marcos
Año de publicación
2022
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bovine alphaherpesvirus 1 (BoHV-1) is a virus known to cause several diseases in cattle, including rhinotracheitis, abortion, among others. Quercetin is a flavonoid that presents therapeutic properties such as antioxidant, antibacterial and antiviral. However, the use of quercetin is limited due to its poor water solubility and its limited bioavailability. This work proposes an oil in water microemulsion based on the use of biocompatible materials as a vehicle to load quercetin. The microemulsion was composed of isopropyl myristate (2.50%); tween 80-ethanol (42.0%) and water (55.5%) and was loaded with quercetin at concentrations that ranged from 50-200 mg L -1 . Particle size (Z) and polidispersity index (PdI) were measured by dynamic light scattering and antiviral activity studies were performed using MDBK (Madin-Darby bovine kidney) cells and BoHV-1Cooper strain (Titer: 106TCID50mL-1 ). A significant reduction (p<0.05) of viral titers after pretreatment of cell monolayers (70.7±1.9)% and in the binding assay (56.8±0.7)%,with respect to the control sample, was achieved when the concentration of quercetin loaded was 100 mgL-1 . The Z and PdI values for this microemulsion were 10.65 nm and 0.137, respectively.
Fil: Volpe, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Grunhut, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
6th International Meeting of Pharmaceutical Sciences
Córdoba
Argentina
Universidad Nacional de Córdoba
Universidad Nacional de Rosario
Materia
MICROEMULSIONS
BOVINE ALFAHERPESVIRUS
QUERCETIN
FLAVONOID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231937
Acceder
id CONICETDig_411afe83ca2373d399a50887ba72fa32
oai_identifier_str oai:ri.conicet.gov.ar:11336/231937
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1Volpe, VerónicaPerez, SandraDolcini, Guillermina LauraBuzzola, Fernanda RoxanaGrunhut, MarcosMICROEMULSIONSBOVINE ALFAHERPESVIRUSQUERCETINFLAVONOIDhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Bovine alphaherpesvirus 1 (BoHV-1) is a virus known to cause several diseases in cattle, including rhinotracheitis, abortion, among others. Quercetin is a flavonoid that presents therapeutic properties such as antioxidant, antibacterial and antiviral. However, the use of quercetin is limited due to its poor water solubility and its limited bioavailability. This work proposes an oil in water microemulsion based on the use of biocompatible materials as a vehicle to load quercetin. The microemulsion was composed of isopropyl myristate (2.50%); tween 80-ethanol (42.0%) and water (55.5%) and was loaded with quercetin at concentrations that ranged from 50-200 mg L -1 . Particle size (Z) and polidispersity index (PdI) were measured by dynamic light scattering and antiviral activity studies were performed using MDBK (Madin-Darby bovine kidney) cells and BoHV-1Cooper strain (Titer: 106TCID50mL-1 ). A significant reduction (p<0.05) of viral titers after pretreatment of cell monolayers (70.7±1.9)% and in the binding assay (56.8±0.7)%,with respect to the control sample, was achieved when the concentration of quercetin loaded was 100 mgL-1 . The Z and PdI values for this microemulsion were 10.65 nm and 0.137, respectively.Fil: Volpe, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Grunhut, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina6th International Meeting of Pharmaceutical SciencesCórdobaArgentinaUniversidad Nacional de CórdobaUniversidad Nacional de RosarioAMG Transcend Association2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231937Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1; 6th International Meeting of Pharmaceutical Sciences; Córdoba; Argentina; 2021; 1-22668-6384CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://conferenceproceedings.international/?page_id=2155info:eu-repo/semantics/altIdentifier/doi/10.33263/Proceedings42.075Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:20:33Zoai:ri.conicet.gov.ar:11336/231937instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:20:34.247CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
title Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
spellingShingle Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
Volpe, Verónica
MICROEMULSIONS
BOVINE ALFAHERPESVIRUS
QUERCETIN
FLAVONOID
title_short Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
title_full Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
title_fullStr Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
title_full_unstemmed Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
title_sort Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1
dc.creator.none.fl_str_mv Volpe, Verónica
Perez, Sandra
Dolcini, Guillermina Laura
Buzzola, Fernanda Roxana
Grunhut, Marcos
author Volpe, Verónica
author_facet Volpe, Verónica
Perez, Sandra
Dolcini, Guillermina Laura
Buzzola, Fernanda Roxana
Grunhut, Marcos
author_role author
author2 Perez, Sandra
Dolcini, Guillermina Laura
Buzzola, Fernanda Roxana
Grunhut, Marcos
author2_role author
author
author
author
dc.subject.none.fl_str_mv MICROEMULSIONS
BOVINE ALFAHERPESVIRUS
QUERCETIN
FLAVONOID
topic MICROEMULSIONS
BOVINE ALFAHERPESVIRUS
QUERCETIN
FLAVONOID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bovine alphaherpesvirus 1 (BoHV-1) is a virus known to cause several diseases in cattle, including rhinotracheitis, abortion, among others. Quercetin is a flavonoid that presents therapeutic properties such as antioxidant, antibacterial and antiviral. However, the use of quercetin is limited due to its poor water solubility and its limited bioavailability. This work proposes an oil in water microemulsion based on the use of biocompatible materials as a vehicle to load quercetin. The microemulsion was composed of isopropyl myristate (2.50%); tween 80-ethanol (42.0%) and water (55.5%) and was loaded with quercetin at concentrations that ranged from 50-200 mg L -1 . Particle size (Z) and polidispersity index (PdI) were measured by dynamic light scattering and antiviral activity studies were performed using MDBK (Madin-Darby bovine kidney) cells and BoHV-1Cooper strain (Titer: 106TCID50mL-1 ). A significant reduction (p<0.05) of viral titers after pretreatment of cell monolayers (70.7±1.9)% and in the binding assay (56.8±0.7)%,with respect to the control sample, was achieved when the concentration of quercetin loaded was 100 mgL-1 . The Z and PdI values for this microemulsion were 10.65 nm and 0.137, respectively.
Fil: Volpe, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Perez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Grunhut, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
6th International Meeting of Pharmaceutical Sciences
Córdoba
Argentina
Universidad Nacional de Córdoba
Universidad Nacional de Rosario
description Bovine alphaherpesvirus 1 (BoHV-1) is a virus known to cause several diseases in cattle, including rhinotracheitis, abortion, among others. Quercetin is a flavonoid that presents therapeutic properties such as antioxidant, antibacterial and antiviral. However, the use of quercetin is limited due to its poor water solubility and its limited bioavailability. This work proposes an oil in water microemulsion based on the use of biocompatible materials as a vehicle to load quercetin. The microemulsion was composed of isopropyl myristate (2.50%); tween 80-ethanol (42.0%) and water (55.5%) and was loaded with quercetin at concentrations that ranged from 50-200 mg L -1 . Particle size (Z) and polidispersity index (PdI) were measured by dynamic light scattering and antiviral activity studies were performed using MDBK (Madin-Darby bovine kidney) cells and BoHV-1Cooper strain (Titer: 106TCID50mL-1 ). A significant reduction (p<0.05) of viral titers after pretreatment of cell monolayers (70.7±1.9)% and in the binding assay (56.8±0.7)%,with respect to the control sample, was achieved when the concentration of quercetin loaded was 100 mgL-1 . The Z and PdI values for this microemulsion were 10.65 nm and 0.137, respectively.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231937
Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1; 6th International Meeting of Pharmaceutical Sciences; Córdoba; Argentina; 2021; 1-2
2668-6384
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231937
identifier_str_mv Quercetin loaded oil in water microemulsion. characterization and evaluation of the antiviral activity against Bovine alphaherpesvirus 1; 6th International Meeting of Pharmaceutical Sciences; Córdoba; Argentina; 2021; 1-2
2668-6384
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://conferenceproceedings.international/?page_id=2155
info:eu-repo/semantics/altIdentifier/doi/10.33263/Proceedings42.075
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv AMG Transcend Association
publisher.none.fl_str_mv AMG Transcend Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847426141267165184
score 13.10058

Publicaciones similares