Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity

Autores
Valli, Adrian Alejandro; Gonzalo Magro, Irene; Sanchez, Diego Hernan
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eukaryotic genomics frequently revealed historical spontaneous endogenization events of external invading nucleic acids, such as viral elements. In plants, an extensive occurrence of endogenous plant pararetroviruses (EPRVs) is usually believed to endow hosts with an additional layer of internal suppressive weaponry. However, an actual demonstration of this activity remains speculative. We analyzed the EPRV component and accompanying silencing effectors of Solanum lycopersicum, documenting that intronic/intergenic pararetroviral integrations bearing inverted-repeats fuel the plant’s RNA-based immune system with suitable transcripts capable of evoking a silencing response. A surprisingly small set of rearrangements explained a substantial fraction of pararetroviral-derived endogenous smallinterfering (si)RNAs, enriched in 22-nt forms typically associated with anti-viral post-transcriptional gene silencing. We provide preliminary evidence that such genetic and immunological signals may be found in other species outside the genus Solanum. Based on molecular dating, bioinformatics, and empirical explorations, we propose that homology-dependent silencing emerging from particular immuno-competent rearranged chromosomal areas that constitute an adaptive heritable trans-acting record of past infections, with potential impact against the unlocking of plant latent EPRVs and cognate-free pararetroviruses.
Fil: Valli, Adrian Alejandro. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Gonzalo Magro, Irene. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Sanchez, Diego Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Materia
CAULIMOVIRIDAE
SOLANUM
ENDOGENIZED PARARETROVIRUS
EPIGENETIC SILENCING
EPIGENETICS
EPRVS
INTRONS
LONG NON-CODING RNA
PARARETROVIRUS
POST-TRANSCRIPTIONAL GENE SILENCING
PTGS
RNA INTERFERENCE
SIRNA
TOMATO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229523
Acceder
id CONICETDig_40bcfc9212193d873f9b1fb431b63711
oai_identifier_str oai:ri.conicet.gov.ar:11336/229523
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based ImmunityValli, Adrian AlejandroGonzalo Magro, IreneSanchez, Diego HernanCAULIMOVIRIDAESOLANUMENDOGENIZED PARARETROVIRUSEPIGENETIC SILENCINGEPIGENETICSEPRVSINTRONSLONG NON-CODING RNAPARARETROVIRUSPOST-TRANSCRIPTIONAL GENE SILENCINGPTGSRNA INTERFERENCESIRNATOMATOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Eukaryotic genomics frequently revealed historical spontaneous endogenization events of external invading nucleic acids, such as viral elements. In plants, an extensive occurrence of endogenous plant pararetroviruses (EPRVs) is usually believed to endow hosts with an additional layer of internal suppressive weaponry. However, an actual demonstration of this activity remains speculative. We analyzed the EPRV component and accompanying silencing effectors of Solanum lycopersicum, documenting that intronic/intergenic pararetroviral integrations bearing inverted-repeats fuel the plant’s RNA-based immune system with suitable transcripts capable of evoking a silencing response. A surprisingly small set of rearrangements explained a substantial fraction of pararetroviral-derived endogenous smallinterfering (si)RNAs, enriched in 22-nt forms typically associated with anti-viral post-transcriptional gene silencing. We provide preliminary evidence that such genetic and immunological signals may be found in other species outside the genus Solanum. Based on molecular dating, bioinformatics, and empirical explorations, we propose that homology-dependent silencing emerging from particular immuno-competent rearranged chromosomal areas that constitute an adaptive heritable trans-acting record of past infections, with potential impact against the unlocking of plant latent EPRVs and cognate-free pararetroviruses.Fil: Valli, Adrian Alejandro. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Gonzalo Magro, Irene. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; EspañaFil: Sanchez, Diego Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaOxford University Press2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229523Valli, Adrian Alejandro; Gonzalo Magro, Irene; Sanchez, Diego Hernan; Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity; Oxford University Press; Molecular Biology and Evolution; 40; 1; 1-2023; 1-171537-1719CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/molbev/msac240info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mbe/article/40/1/msac240/6794085info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:09Zoai:ri.conicet.gov.ar:11336/229523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:10.053CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
title Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
spellingShingle Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
Valli, Adrian Alejandro
CAULIMOVIRIDAE
SOLANUM
ENDOGENIZED PARARETROVIRUS
EPIGENETIC SILENCING
EPIGENETICS
EPRVS
INTRONS
LONG NON-CODING RNA
PARARETROVIRUS
POST-TRANSCRIPTIONAL GENE SILENCING
PTGS
RNA INTERFERENCE
SIRNA
TOMATO
title_short Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
title_full Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
title_fullStr Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
title_full_unstemmed Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
title_sort Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity
dc.creator.none.fl_str_mv Valli, Adrian Alejandro
Gonzalo Magro, Irene
Sanchez, Diego Hernan
author Valli, Adrian Alejandro
author_facet Valli, Adrian Alejandro
Gonzalo Magro, Irene
Sanchez, Diego Hernan
author_role author
author2 Gonzalo Magro, Irene
Sanchez, Diego Hernan
author2_role author
author
dc.subject.none.fl_str_mv CAULIMOVIRIDAE
SOLANUM
ENDOGENIZED PARARETROVIRUS
EPIGENETIC SILENCING
EPIGENETICS
EPRVS
INTRONS
LONG NON-CODING RNA
PARARETROVIRUS
POST-TRANSCRIPTIONAL GENE SILENCING
PTGS
RNA INTERFERENCE
SIRNA
TOMATO
topic CAULIMOVIRIDAE
SOLANUM
ENDOGENIZED PARARETROVIRUS
EPIGENETIC SILENCING
EPIGENETICS
EPRVS
INTRONS
LONG NON-CODING RNA
PARARETROVIRUS
POST-TRANSCRIPTIONAL GENE SILENCING
PTGS
RNA INTERFERENCE
SIRNA
TOMATO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Eukaryotic genomics frequently revealed historical spontaneous endogenization events of external invading nucleic acids, such as viral elements. In plants, an extensive occurrence of endogenous plant pararetroviruses (EPRVs) is usually believed to endow hosts with an additional layer of internal suppressive weaponry. However, an actual demonstration of this activity remains speculative. We analyzed the EPRV component and accompanying silencing effectors of Solanum lycopersicum, documenting that intronic/intergenic pararetroviral integrations bearing inverted-repeats fuel the plant’s RNA-based immune system with suitable transcripts capable of evoking a silencing response. A surprisingly small set of rearrangements explained a substantial fraction of pararetroviral-derived endogenous smallinterfering (si)RNAs, enriched in 22-nt forms typically associated with anti-viral post-transcriptional gene silencing. We provide preliminary evidence that such genetic and immunological signals may be found in other species outside the genus Solanum. Based on molecular dating, bioinformatics, and empirical explorations, we propose that homology-dependent silencing emerging from particular immuno-competent rearranged chromosomal areas that constitute an adaptive heritable trans-acting record of past infections, with potential impact against the unlocking of plant latent EPRVs and cognate-free pararetroviruses.
Fil: Valli, Adrian Alejandro. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Gonzalo Magro, Irene. Consejo Superior de Investigaciones Científicas. Centro Nacional de Biotecnología; España
Fil: Sanchez, Diego Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
description Eukaryotic genomics frequently revealed historical spontaneous endogenization events of external invading nucleic acids, such as viral elements. In plants, an extensive occurrence of endogenous plant pararetroviruses (EPRVs) is usually believed to endow hosts with an additional layer of internal suppressive weaponry. However, an actual demonstration of this activity remains speculative. We analyzed the EPRV component and accompanying silencing effectors of Solanum lycopersicum, documenting that intronic/intergenic pararetroviral integrations bearing inverted-repeats fuel the plant’s RNA-based immune system with suitable transcripts capable of evoking a silencing response. A surprisingly small set of rearrangements explained a substantial fraction of pararetroviral-derived endogenous smallinterfering (si)RNAs, enriched in 22-nt forms typically associated with anti-viral post-transcriptional gene silencing. We provide preliminary evidence that such genetic and immunological signals may be found in other species outside the genus Solanum. Based on molecular dating, bioinformatics, and empirical explorations, we propose that homology-dependent silencing emerging from particular immuno-competent rearranged chromosomal areas that constitute an adaptive heritable trans-acting record of past infections, with potential impact against the unlocking of plant latent EPRVs and cognate-free pararetroviruses.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229523
Valli, Adrian Alejandro; Gonzalo Magro, Irene; Sanchez, Diego Hernan; Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity; Oxford University Press; Molecular Biology and Evolution; 40; 1; 1-2023; 1-17
1537-1719
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229523
identifier_str_mv Valli, Adrian Alejandro; Gonzalo Magro, Irene; Sanchez, Diego Hernan; Rearranged Endogenized Plant Pararetroviruses as Evidence of Heritable RNA-based Immunity; Oxford University Press; Molecular Biology and Evolution; 40; 1; 1-2023; 1-17
1537-1719
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/molbev/msac240
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/mbe/article/40/1/msac240/6794085
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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