Measurement of charge distribution in actin bundles by surface potential microscopy

Autores
Zhang, Peng; Cantiello, Horacio Fabio
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bundles of filamentous actin (F-actin) deposited on a gold-plated surface were concurrently imaged using atomic force microscopy (AFM) and surface potential microscopy (SPM). The surface potential was mapped as a function of tip distance to surface using a constant bias potential. There was an uneven spatial distribution of charges detected by SPM, consistent with the segmented topological features shown by AFM of the actin bundles. SPM analysis showed localized changes in surface potential between the axial and transversal sections of the bundles, which are consistent with nonuniform charge distributions of adsorbed salt ions on F-actin.
Fil: Zhang, Peng. Harvard Medical School; Estados Unidos
Fil: Cantiello, Horacio Fabio. Harvard Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
actin bundles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242039
Acceder
id CONICETDig_40946ddf8a66baff610f4d810838f224
oai_identifier_str oai:ri.conicet.gov.ar:11336/242039
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Measurement of charge distribution in actin bundles by surface potential microscopyZhang, PengCantiello, Horacio Fabioactin bundleshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bundles of filamentous actin (F-actin) deposited on a gold-plated surface were concurrently imaged using atomic force microscopy (AFM) and surface potential microscopy (SPM). The surface potential was mapped as a function of tip distance to surface using a constant bias potential. There was an uneven spatial distribution of charges detected by SPM, consistent with the segmented topological features shown by AFM of the actin bundles. SPM analysis showed localized changes in surface potential between the axial and transversal sections of the bundles, which are consistent with nonuniform charge distributions of adsorbed salt ions on F-actin.Fil: Zhang, Peng. Harvard Medical School; Estados UnidosFil: Cantiello, Horacio Fabio. Harvard Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaAmerican Institute of Physics2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242039Zhang, Peng; Cantiello, Horacio Fabio; Measurement of charge distribution in actin bundles by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 3; 7-2009; 33701-337020003-6951CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1063/1.3167281info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:11Zoai:ri.conicet.gov.ar:11336/242039instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:11.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Measurement of charge distribution in actin bundles by surface potential microscopy
title Measurement of charge distribution in actin bundles by surface potential microscopy
spellingShingle Measurement of charge distribution in actin bundles by surface potential microscopy
Zhang, Peng
actin bundles
title_short Measurement of charge distribution in actin bundles by surface potential microscopy
title_full Measurement of charge distribution in actin bundles by surface potential microscopy
title_fullStr Measurement of charge distribution in actin bundles by surface potential microscopy
title_full_unstemmed Measurement of charge distribution in actin bundles by surface potential microscopy
title_sort Measurement of charge distribution in actin bundles by surface potential microscopy
dc.creator.none.fl_str_mv Zhang, Peng
Cantiello, Horacio Fabio
author Zhang, Peng
author_facet Zhang, Peng
Cantiello, Horacio Fabio
author_role author
author2 Cantiello, Horacio Fabio
author2_role author
dc.subject.none.fl_str_mv actin bundles
topic actin bundles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bundles of filamentous actin (F-actin) deposited on a gold-plated surface were concurrently imaged using atomic force microscopy (AFM) and surface potential microscopy (SPM). The surface potential was mapped as a function of tip distance to surface using a constant bias potential. There was an uneven spatial distribution of charges detected by SPM, consistent with the segmented topological features shown by AFM of the actin bundles. SPM analysis showed localized changes in surface potential between the axial and transversal sections of the bundles, which are consistent with nonuniform charge distributions of adsorbed salt ions on F-actin.
Fil: Zhang, Peng. Harvard Medical School; Estados Unidos
Fil: Cantiello, Horacio Fabio. Harvard Medical School; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Bundles of filamentous actin (F-actin) deposited on a gold-plated surface were concurrently imaged using atomic force microscopy (AFM) and surface potential microscopy (SPM). The surface potential was mapped as a function of tip distance to surface using a constant bias potential. There was an uneven spatial distribution of charges detected by SPM, consistent with the segmented topological features shown by AFM of the actin bundles. SPM analysis showed localized changes in surface potential between the axial and transversal sections of the bundles, which are consistent with nonuniform charge distributions of adsorbed salt ions on F-actin.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242039
Zhang, Peng; Cantiello, Horacio Fabio; Measurement of charge distribution in actin bundles by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 3; 7-2009; 33701-33702
0003-6951
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242039
identifier_str_mv Zhang, Peng; Cantiello, Horacio Fabio; Measurement of charge distribution in actin bundles by surface potential microscopy; American Institute of Physics; Applied Physics Letters; 95; 3; 7-2009; 33701-33702
0003-6951
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1063/1.3167281
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Institute of Physics
publisher.none.fl_str_mv American Institute of Physics
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270035176849408
score 13.13397

Publicaciones similares