Genome-wide association study of resistance to Mal de Río Cuarto disease in maize

Autores
Rossi, Ezequiel Alejandro; Ruiz, Marcos; Bonamico, Natalia Cecilia; Balzarini, Monica Graciela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Argentine maize has been extensively screened for incidence (INC) and severity (SEV) of Mal de Río Cuarto disease (MRC), caused by Mal de Río Cuarto virus (MRCV), family Reoviridae, genus Fijivirus, narrowing the breeding genetic basis. Both traits are highly heritable phenotypic measurements, and quantify the strong disease impact on grain yield. The adaptation of exotic germplasm to variation of those traits has not been explored. The aim of this work was to identify, in a non-local and diverse panel of maize inbred lines, novel genomic regions associated with resistance to MRC. First, we phenotyped 206 maize inbred lines from the International Maize and Wheat Improvement Center (CIMMYT), in several environments of the MRC-endemic area under natural virus infection, to obtain the best linear unbiased predictor (BLUP) of line effects regarding INC and SEV. A multi-environment and multi-trait mixed linear model was fitted to derive the multivariate BLUPs. Genetic variance and mean-basis heritability were high in both traits and a significant genetic correlation among them was found. Second, we performed a genome-wide association study (GWAS) by linking the BLUPs with 78,376 SNP markers available for 186 lines. The GWAS identified new alleles for resistance to MRC in six genomic regions from the exotic germoplasm. Four of them reduce symultaneously the appearance and severity of disease symptoms. Improved susceptible parental lines through marker-assisted recurrent selection would allow us to increase the resistance of maize hybrids to MRC disease.
Fil: Rossi, Ezequiel Alejandro. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Ruiz, Marcos. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Bonamico, Natalia Cecilia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Balzarini, Monica Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Desarrollo Rural. Area de Estadística y Biometría; Argentina
Materia
Exotic germplasm
Maize inbred lines
Mal de Rio Cuarto
Resistance alleles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146047
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/146047
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome-wide association study of resistance to Mal de Río Cuarto disease in maizeRossi, Ezequiel AlejandroRuiz, MarcosBonamico, Natalia CeciliaBalzarini, Monica GracielaExotic germplasmMaize inbred linesMal de Rio CuartoResistance alleleshttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Argentine maize has been extensively screened for incidence (INC) and severity (SEV) of Mal de Río Cuarto disease (MRC), caused by Mal de Río Cuarto virus (MRCV), family Reoviridae, genus Fijivirus, narrowing the breeding genetic basis. Both traits are highly heritable phenotypic measurements, and quantify the strong disease impact on grain yield. The adaptation of exotic germplasm to variation of those traits has not been explored. The aim of this work was to identify, in a non-local and diverse panel of maize inbred lines, novel genomic regions associated with resistance to MRC. First, we phenotyped 206 maize inbred lines from the International Maize and Wheat Improvement Center (CIMMYT), in several environments of the MRC-endemic area under natural virus infection, to obtain the best linear unbiased predictor (BLUP) of line effects regarding INC and SEV. A multi-environment and multi-trait mixed linear model was fitted to derive the multivariate BLUPs. Genetic variance and mean-basis heritability were high in both traits and a significant genetic correlation among them was found. Second, we performed a genome-wide association study (GWAS) by linking the BLUPs with 78,376 SNP markers available for 186 lines. The GWAS identified new alleles for resistance to MRC in six genomic regions from the exotic germoplasm. Four of them reduce symultaneously the appearance and severity of disease symptoms. Improved susceptible parental lines through marker-assisted recurrent selection would allow us to increase the resistance of maize hybrids to MRC disease.Fil: Rossi, Ezequiel Alejandro. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Ruiz, Marcos. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Bonamico, Natalia Cecilia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; ArgentinaFil: Balzarini, Monica Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Desarrollo Rural. Area de Estadística y Biometría; ArgentinaAmerican Society of Agronomy2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146047Rossi, Ezequiel Alejandro; Ruiz, Marcos; Bonamico, Natalia Cecilia; Balzarini, Monica Graciela; Genome-wide association study of resistance to Mal de Río Cuarto disease in maize; American Society of Agronomy; Agronomy Journal; 112; 6; 11-2020; 4624-46330002-1962CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://acsess.onlinelibrary.wiley.com/doi/full/10.1002/agj2.20448info:eu-repo/semantics/altIdentifier/doi/10.1002/agj2.20448info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:03Zoai:ri.conicet.gov.ar:11336/146047instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:03.619CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
title Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
spellingShingle Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
Rossi, Ezequiel Alejandro
Exotic germplasm
Maize inbred lines
Mal de Rio Cuarto
Resistance alleles
title_short Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
title_full Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
title_fullStr Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
title_full_unstemmed Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
title_sort Genome-wide association study of resistance to Mal de Río Cuarto disease in maize
dc.creator.none.fl_str_mv Rossi, Ezequiel Alejandro
Ruiz, Marcos
Bonamico, Natalia Cecilia
Balzarini, Monica Graciela
author Rossi, Ezequiel Alejandro
author_facet Rossi, Ezequiel Alejandro
Ruiz, Marcos
Bonamico, Natalia Cecilia
Balzarini, Monica Graciela
author_role author
author2 Ruiz, Marcos
Bonamico, Natalia Cecilia
Balzarini, Monica Graciela
author2_role author
author
author
dc.subject.none.fl_str_mv Exotic germplasm
Maize inbred lines
Mal de Rio Cuarto
Resistance alleles
topic Exotic germplasm
Maize inbred lines
Mal de Rio Cuarto
Resistance alleles
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Argentine maize has been extensively screened for incidence (INC) and severity (SEV) of Mal de Río Cuarto disease (MRC), caused by Mal de Río Cuarto virus (MRCV), family Reoviridae, genus Fijivirus, narrowing the breeding genetic basis. Both traits are highly heritable phenotypic measurements, and quantify the strong disease impact on grain yield. The adaptation of exotic germplasm to variation of those traits has not been explored. The aim of this work was to identify, in a non-local and diverse panel of maize inbred lines, novel genomic regions associated with resistance to MRC. First, we phenotyped 206 maize inbred lines from the International Maize and Wheat Improvement Center (CIMMYT), in several environments of the MRC-endemic area under natural virus infection, to obtain the best linear unbiased predictor (BLUP) of line effects regarding INC and SEV. A multi-environment and multi-trait mixed linear model was fitted to derive the multivariate BLUPs. Genetic variance and mean-basis heritability were high in both traits and a significant genetic correlation among them was found. Second, we performed a genome-wide association study (GWAS) by linking the BLUPs with 78,376 SNP markers available for 186 lines. The GWAS identified new alleles for resistance to MRC in six genomic regions from the exotic germoplasm. Four of them reduce symultaneously the appearance and severity of disease symptoms. Improved susceptible parental lines through marker-assisted recurrent selection would allow us to increase the resistance of maize hybrids to MRC disease.
Fil: Rossi, Ezequiel Alejandro. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Ruiz, Marcos. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Bonamico, Natalia Cecilia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Investigaciones Agrobiotecnologicas. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Investigaciones Agrobiotecnologicas.; Argentina
Fil: Balzarini, Monica Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Desarrollo Rural. Area de Estadística y Biometría; Argentina
description Argentine maize has been extensively screened for incidence (INC) and severity (SEV) of Mal de Río Cuarto disease (MRC), caused by Mal de Río Cuarto virus (MRCV), family Reoviridae, genus Fijivirus, narrowing the breeding genetic basis. Both traits are highly heritable phenotypic measurements, and quantify the strong disease impact on grain yield. The adaptation of exotic germplasm to variation of those traits has not been explored. The aim of this work was to identify, in a non-local and diverse panel of maize inbred lines, novel genomic regions associated with resistance to MRC. First, we phenotyped 206 maize inbred lines from the International Maize and Wheat Improvement Center (CIMMYT), in several environments of the MRC-endemic area under natural virus infection, to obtain the best linear unbiased predictor (BLUP) of line effects regarding INC and SEV. A multi-environment and multi-trait mixed linear model was fitted to derive the multivariate BLUPs. Genetic variance and mean-basis heritability were high in both traits and a significant genetic correlation among them was found. Second, we performed a genome-wide association study (GWAS) by linking the BLUPs with 78,376 SNP markers available for 186 lines. The GWAS identified new alleles for resistance to MRC in six genomic regions from the exotic germoplasm. Four of them reduce symultaneously the appearance and severity of disease symptoms. Improved susceptible parental lines through marker-assisted recurrent selection would allow us to increase the resistance of maize hybrids to MRC disease.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146047
Rossi, Ezequiel Alejandro; Ruiz, Marcos; Bonamico, Natalia Cecilia; Balzarini, Monica Graciela; Genome-wide association study of resistance to Mal de Río Cuarto disease in maize; American Society of Agronomy; Agronomy Journal; 112; 6; 11-2020; 4624-4633
0002-1962
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146047
identifier_str_mv Rossi, Ezequiel Alejandro; Ruiz, Marcos; Bonamico, Natalia Cecilia; Balzarini, Monica Graciela; Genome-wide association study of resistance to Mal de Río Cuarto disease in maize; American Society of Agronomy; Agronomy Journal; 112; 6; 11-2020; 4624-4633
0002-1962
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://acsess.onlinelibrary.wiley.com/doi/full/10.1002/agj2.20448
info:eu-repo/semantics/altIdentifier/doi/10.1002/agj2.20448
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Agronomy
publisher.none.fl_str_mv American Society of Agronomy
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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