Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives

Autores
Trelles, Jorge Abel; Lapponi, María José
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract: Background: Nucleoside analogue (NAs) derivatives comprise a large family of pharmaceuticals clinically used as antitumoral and antiviral compounds. Originally, the production of NAs involved chemical synthesis, but a greener bioproduction alternative exists and involves the use of enzymes that catalyze transglycosylation reactions between modified purinic or pyrimidinic bases and sugars. To be considered as an option for industrial application, it is vital to immobilize these biocatalysts. Methods: This article describes current methodologies for whole cell and protein immobilization mostly applied to the synthesis of important NAs. Immobilization describes ways of cell or enzyme confinement in diverse surfaces or matrixes. It is important to be familiar with the variety of matrixes and supports available prior to biocatalyst immobilization so the most adequate can be selected for the purpose sought. Results: From the different articles compiled, it can be acknowledged that the main methods for protein or cell stabilization are immobilization by adsorption, covalent, cross-linking and entrapment. The most widely used matrixes and supports are agar, alginate, polyacrylamide, sepharose derivatives, and acrylic resins, among others.
Protein or cell stabilization has the advantage of stabilizing immobilization, favoring their facile separation from the reaction medium for further reuse and also making the purification of the final product easier. Moreover, biocatalyst stabilization allows a facile estimation of the economic cost of the bioprocess and of an eventual scale-up, being a basic requirement for industrial application.
Conclusion: In order to achieve successful biocatalyst immobilization, parameters such as biocatalyst stability,mechanical resistance, and reusability should be considered. This review describes and summarizes the methods used for the immobilization of biocatalysts for the synthesis of NAs in the last years.
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Lapponi, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Materia
Entrapment
Covalent Bonding
Nanoclay
Hydrogel
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41660
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41660
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivativesTrelles, Jorge AbelLapponi, María JoséEntrapmentCovalent BondingNanoclayHydrogelhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Abstract: Background: Nucleoside analogue (NAs) derivatives comprise a large family of pharmaceuticals clinically used as antitumoral and antiviral compounds. Originally, the production of NAs involved chemical synthesis, but a greener bioproduction alternative exists and involves the use of enzymes that catalyze transglycosylation reactions between modified purinic or pyrimidinic bases and sugars. To be considered as an option for industrial application, it is vital to immobilize these biocatalysts. Methods: This article describes current methodologies for whole cell and protein immobilization mostly applied to the synthesis of important NAs. Immobilization describes ways of cell or enzyme confinement in diverse surfaces or matrixes. It is important to be familiar with the variety of matrixes and supports available prior to biocatalyst immobilization so the most adequate can be selected for the purpose sought. Results: From the different articles compiled, it can be acknowledged that the main methods for protein or cell stabilization are immobilization by adsorption, covalent, cross-linking and entrapment. The most widely used matrixes and supports are agar, alginate, polyacrylamide, sepharose derivatives, and acrylic resins, among others.<br />Protein or cell stabilization has the advantage of stabilizing immobilization, favoring their facile separation from the reaction medium for further reuse and also making the purification of the final product easier. Moreover, biocatalyst stabilization allows a facile estimation of the economic cost of the bioprocess and of an eventual scale-up, being a basic requirement for industrial application.<br />Conclusion: In order to achieve successful biocatalyst immobilization, parameters such as biocatalyst stability,mechanical resistance, and reusability should be considered. This review describes and summarizes the methods used for the immobilization of biocatalysts for the synthesis of NAs in the last years.Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaFil: Lapponi, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaBentham Science Publishers2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/41660Trelles, Jorge Abel; Lapponi, María José; Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives; Bentham Science Publishers; Current Pharmaceutical Design; 24; 12-20171381-6128CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2174/1381612824666171204102204info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/158036/articleinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:17Zoai:ri.conicet.gov.ar:11336/41660instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:18.229CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
title Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
spellingShingle Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
Trelles, Jorge Abel
Entrapment
Covalent Bonding
Nanoclay
Hydrogel
title_short Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
title_full Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
title_fullStr Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
title_full_unstemmed Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
title_sort Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives
dc.creator.none.fl_str_mv Trelles, Jorge Abel
Lapponi, María José
author Trelles, Jorge Abel
author_facet Trelles, Jorge Abel
Lapponi, María José
author_role author
author2 Lapponi, María José
author2_role author
dc.subject.none.fl_str_mv Entrapment
Covalent Bonding
Nanoclay
Hydrogel
topic Entrapment
Covalent Bonding
Nanoclay
Hydrogel
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Abstract: Background: Nucleoside analogue (NAs) derivatives comprise a large family of pharmaceuticals clinically used as antitumoral and antiviral compounds. Originally, the production of NAs involved chemical synthesis, but a greener bioproduction alternative exists and involves the use of enzymes that catalyze transglycosylation reactions between modified purinic or pyrimidinic bases and sugars. To be considered as an option for industrial application, it is vital to immobilize these biocatalysts. Methods: This article describes current methodologies for whole cell and protein immobilization mostly applied to the synthesis of important NAs. Immobilization describes ways of cell or enzyme confinement in diverse surfaces or matrixes. It is important to be familiar with the variety of matrixes and supports available prior to biocatalyst immobilization so the most adequate can be selected for the purpose sought. Results: From the different articles compiled, it can be acknowledged that the main methods for protein or cell stabilization are immobilization by adsorption, covalent, cross-linking and entrapment. The most widely used matrixes and supports are agar, alginate, polyacrylamide, sepharose derivatives, and acrylic resins, among others.<br />Protein or cell stabilization has the advantage of stabilizing immobilization, favoring their facile separation from the reaction medium for further reuse and also making the purification of the final product easier. Moreover, biocatalyst stabilization allows a facile estimation of the economic cost of the bioprocess and of an eventual scale-up, being a basic requirement for industrial application.<br />Conclusion: In order to achieve successful biocatalyst immobilization, parameters such as biocatalyst stability,mechanical resistance, and reusability should be considered. This review describes and summarizes the methods used for the immobilization of biocatalysts for the synthesis of NAs in the last years.
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Lapponi, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
description Abstract: Background: Nucleoside analogue (NAs) derivatives comprise a large family of pharmaceuticals clinically used as antitumoral and antiviral compounds. Originally, the production of NAs involved chemical synthesis, but a greener bioproduction alternative exists and involves the use of enzymes that catalyze transglycosylation reactions between modified purinic or pyrimidinic bases and sugars. To be considered as an option for industrial application, it is vital to immobilize these biocatalysts. Methods: This article describes current methodologies for whole cell and protein immobilization mostly applied to the synthesis of important NAs. Immobilization describes ways of cell or enzyme confinement in diverse surfaces or matrixes. It is important to be familiar with the variety of matrixes and supports available prior to biocatalyst immobilization so the most adequate can be selected for the purpose sought. Results: From the different articles compiled, it can be acknowledged that the main methods for protein or cell stabilization are immobilization by adsorption, covalent, cross-linking and entrapment. The most widely used matrixes and supports are agar, alginate, polyacrylamide, sepharose derivatives, and acrylic resins, among others.<br />Protein or cell stabilization has the advantage of stabilizing immobilization, favoring their facile separation from the reaction medium for further reuse and also making the purification of the final product easier. Moreover, biocatalyst stabilization allows a facile estimation of the economic cost of the bioprocess and of an eventual scale-up, being a basic requirement for industrial application.<br />Conclusion: In order to achieve successful biocatalyst immobilization, parameters such as biocatalyst stability,mechanical resistance, and reusability should be considered. This review describes and summarizes the methods used for the immobilization of biocatalysts for the synthesis of NAs in the last years.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41660
Trelles, Jorge Abel; Lapponi, María José; Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives; Bentham Science Publishers; Current Pharmaceutical Design; 24; 12-2017
1381-6128
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41660
identifier_str_mv Trelles, Jorge Abel; Lapponi, María José; Immobilization techniques applied to the development of biocatalysts for the synthesis of nucleoside analogue derivatives; Bentham Science Publishers; Current Pharmaceutical Design; 24; 12-2017
1381-6128
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2174/1381612824666171204102204
info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/158036/article
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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