Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications

Autores
Moreno, Natalia; Scheverin, Verónica Natalia; Agotegaray, Mariela Alejandra; Lassalle, Verónica Leticia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Coating of iron oxide magnetic nanoparticles (IOMNPs) with biocompatible molecules is mandatory for biomedical applications. The election of amino acids as coating agents is because their ability in surface stabilization of IOMNPs being also inexpensive and nontoxic. In the present work, glutamic acid (GA) was selected as coating agent aiming to obtain GA-modified IOMNPs with suitable physicochemical properties, including stability, for biomedical applications. The co-precipitation method was applied to obtain IOMNPs functionalized with GA. Two experimental procedures were evaluated varying the order of GA incorporation. In the first procedure (1), an aqueous solution of GA was added under magnetic stirring to a mixture of FeCl3.6H2O and FeSO4. In the second case (2), the mixture of iron salts was added to the aqueous solution of GA. Both reactions were conducted under N2 atmosphere at 70 °C. A solution of NaOH was added dropwise. After 30 min, the supernatants were removed, and the resultant solids were washed and dried at 45 °C. The same procedure was applied to obtain bare IOMNPs. The resultant formulations were studied by FTIR, DLS to determine hydrodynamic diameter and isoelectric point, TEM, atomic absorption spectroscopy (for iron content determination) and DRX. Data analysis revealed that the method 2 allowed the efficient functionalization of IOMNPs with GA rendering a formulation with hydrodynamic diameter of 226.0 nm with spherical shape. The first method renders IOMNPs with similar properties than bare IOMNPs, revealing that functionalization with GA was not successful. The method applied to functionalize IOMNPs with GA was dependent on the order of reactants aggregation. It was possible to obtain IOMNPs coated with GA (IOMNPs@GA) with suitable properties for biomedical applications. The exposure of functional groups such as carboxylate moieties allows to the potential anchoring of diverse molecules (drugs or biomolecules) for the located treatment of multiple diseases.
Fil: Moreno, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Scheverin, Verónica Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Reunión Anual de Sociedades de Biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Asociación Argentina de Farmacología Experimental
Materia
MAGNETIC NANOPARTICLES
GLUTAMIC ACID
BIOMEDICINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221512
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applicationsMoreno, NataliaScheverin, Verónica NataliaAgotegaray, Mariela AlejandraLassalle, Verónica LeticiaMAGNETIC NANOPARTICLESGLUTAMIC ACIDBIOMEDICINEhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Coating of iron oxide magnetic nanoparticles (IOMNPs) with biocompatible molecules is mandatory for biomedical applications. The election of amino acids as coating agents is because their ability in surface stabilization of IOMNPs being also inexpensive and nontoxic. In the present work, glutamic acid (GA) was selected as coating agent aiming to obtain GA-modified IOMNPs with suitable physicochemical properties, including stability, for biomedical applications. The co-precipitation method was applied to obtain IOMNPs functionalized with GA. Two experimental procedures were evaluated varying the order of GA incorporation. In the first procedure (1), an aqueous solution of GA was added under magnetic stirring to a mixture of FeCl3.6H2O and FeSO4. In the second case (2), the mixture of iron salts was added to the aqueous solution of GA. Both reactions were conducted under N2 atmosphere at 70 °C. A solution of NaOH was added dropwise. After 30 min, the supernatants were removed, and the resultant solids were washed and dried at 45 °C. The same procedure was applied to obtain bare IOMNPs. The resultant formulations were studied by FTIR, DLS to determine hydrodynamic diameter and isoelectric point, TEM, atomic absorption spectroscopy (for iron content determination) and DRX. Data analysis revealed that the method 2 allowed the efficient functionalization of IOMNPs with GA rendering a formulation with hydrodynamic diameter of 226.0 nm with spherical shape. The first method renders IOMNPs with similar properties than bare IOMNPs, revealing that functionalization with GA was not successful. The method applied to functionalize IOMNPs with GA was dependent on the order of reactants aggregation. It was possible to obtain IOMNPs coated with GA (IOMNPs@GA) with suitable properties for biomedical applications. The exposure of functional groups such as carboxylate moieties allows to the potential anchoring of diverse molecules (drugs or biomolecules) for the located treatment of multiple diseases.Fil: Moreno, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Scheverin, Verónica Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaReunión Anual de Sociedades de Biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioAsociación Argentina de Farmacología ExperimentalFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSeminarioJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221512Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications; Reunión Anual de Sociedades de Biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 279-2790025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:45Zoai:ri.conicet.gov.ar:11336/221512instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:46.047CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
title Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
spellingShingle Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
Moreno, Natalia
MAGNETIC NANOPARTICLES
GLUTAMIC ACID
BIOMEDICINE
title_short Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
title_full Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
title_fullStr Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
title_full_unstemmed Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
title_sort Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications
dc.creator.none.fl_str_mv Moreno, Natalia
Scheverin, Verónica Natalia
Agotegaray, Mariela Alejandra
Lassalle, Verónica Leticia
author Moreno, Natalia
author_facet Moreno, Natalia
Scheverin, Verónica Natalia
Agotegaray, Mariela Alejandra
Lassalle, Verónica Leticia
author_role author
author2 Scheverin, Verónica Natalia
Agotegaray, Mariela Alejandra
Lassalle, Verónica Leticia
author2_role author
author
author
dc.subject.none.fl_str_mv MAGNETIC NANOPARTICLES
GLUTAMIC ACID
BIOMEDICINE
topic MAGNETIC NANOPARTICLES
GLUTAMIC ACID
BIOMEDICINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Coating of iron oxide magnetic nanoparticles (IOMNPs) with biocompatible molecules is mandatory for biomedical applications. The election of amino acids as coating agents is because their ability in surface stabilization of IOMNPs being also inexpensive and nontoxic. In the present work, glutamic acid (GA) was selected as coating agent aiming to obtain GA-modified IOMNPs with suitable physicochemical properties, including stability, for biomedical applications. The co-precipitation method was applied to obtain IOMNPs functionalized with GA. Two experimental procedures were evaluated varying the order of GA incorporation. In the first procedure (1), an aqueous solution of GA was added under magnetic stirring to a mixture of FeCl3.6H2O and FeSO4. In the second case (2), the mixture of iron salts was added to the aqueous solution of GA. Both reactions were conducted under N2 atmosphere at 70 °C. A solution of NaOH was added dropwise. After 30 min, the supernatants were removed, and the resultant solids were washed and dried at 45 °C. The same procedure was applied to obtain bare IOMNPs. The resultant formulations were studied by FTIR, DLS to determine hydrodynamic diameter and isoelectric point, TEM, atomic absorption spectroscopy (for iron content determination) and DRX. Data analysis revealed that the method 2 allowed the efficient functionalization of IOMNPs with GA rendering a formulation with hydrodynamic diameter of 226.0 nm with spherical shape. The first method renders IOMNPs with similar properties than bare IOMNPs, revealing that functionalization with GA was not successful. The method applied to functionalize IOMNPs with GA was dependent on the order of reactants aggregation. It was possible to obtain IOMNPs coated with GA (IOMNPs@GA) with suitable properties for biomedical applications. The exposure of functional groups such as carboxylate moieties allows to the potential anchoring of diverse molecules (drugs or biomolecules) for the located treatment of multiple diseases.
Fil: Moreno, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Scheverin, Verónica Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Reunión Anual de Sociedades de Biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Asociación Argentina de Farmacología Experimental
description Coating of iron oxide magnetic nanoparticles (IOMNPs) with biocompatible molecules is mandatory for biomedical applications. The election of amino acids as coating agents is because their ability in surface stabilization of IOMNPs being also inexpensive and nontoxic. In the present work, glutamic acid (GA) was selected as coating agent aiming to obtain GA-modified IOMNPs with suitable physicochemical properties, including stability, for biomedical applications. The co-precipitation method was applied to obtain IOMNPs functionalized with GA. Two experimental procedures were evaluated varying the order of GA incorporation. In the first procedure (1), an aqueous solution of GA was added under magnetic stirring to a mixture of FeCl3.6H2O and FeSO4. In the second case (2), the mixture of iron salts was added to the aqueous solution of GA. Both reactions were conducted under N2 atmosphere at 70 °C. A solution of NaOH was added dropwise. After 30 min, the supernatants were removed, and the resultant solids were washed and dried at 45 °C. The same procedure was applied to obtain bare IOMNPs. The resultant formulations were studied by FTIR, DLS to determine hydrodynamic diameter and isoelectric point, TEM, atomic absorption spectroscopy (for iron content determination) and DRX. Data analysis revealed that the method 2 allowed the efficient functionalization of IOMNPs with GA rendering a formulation with hydrodynamic diameter of 226.0 nm with spherical shape. The first method renders IOMNPs with similar properties than bare IOMNPs, revealing that functionalization with GA was not successful. The method applied to functionalize IOMNPs with GA was dependent on the order of reactants aggregation. It was possible to obtain IOMNPs coated with GA (IOMNPs@GA) with suitable properties for biomedical applications. The exposure of functional groups such as carboxylate moieties allows to the potential anchoring of diverse molecules (drugs or biomolecules) for the located treatment of multiple diseases.
publishDate 2020
dc.date.none.fl_str_mv 2020
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221512
Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications; Reunión Anual de Sociedades de Biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 279-279
0025-7680
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CONICET Digital
CONICET
url http://hdl.handle.net/11336/221512
identifier_str_mv Glutamic acid as coating for magnetic nanoparticles: a platform for diverse biomedical applications; Reunión Anual de Sociedades de Biociencia 2019: LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); LI Reunión Anual de la Asociación Argentina de Farmacología Experimental (SAFE); XXI Reunión Anual de la Sociedad Argentina de Biología (SAB); XXXI Reunión Anual de la Sociedad Argentina de Protozoología (SAP); IX Reunión Anual de la Asociación Argentina de Nanomedicinas (NANOMED-ar); VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (AACyTAL); Mar del Plata; Argentina; 2019; 279-279
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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