Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant

Autores
Rivas Aravena, Andrea; Fuentes, Jazmin; Cartagena, Julio; Brito, Tania; Poggio, Thelma Veronica; la Torre, Jose Leonardo; Mendoza, Hegaly; Gonzalez Nilo, Fernando Danilo; Sandino, Ana María; Eugenio, Emilio Osvaldo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.
Fil: Rivas Aravena, Andrea. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile. Comision Chilena de Energía Nuclear. Departamento de Aplicaciones Nucleares; Chile
Fil: Fuentes, Jazmin. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Cartagena, Julio. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Brito, Tania. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina. Tecnovax; Argentina
Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina. Tecnovax; Argentina
Fil: Mendoza, Hegaly. Universidad Andr és Bello. Center for Bioinformatics and Integrative Biology; Chile
Fil: Gonzalez Nilo, Fernando Danilo. Universidad Andr és Bello. Center for Bioinformatics and Integrative Biology; Chile
Fil: Sandino, Ana María. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Eugenio, Emilio Osvaldo. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Materia
Nanoparticles
Adjuvant
Vaccine
Atlantic Salmon
Salmon Alphavirus
Isav
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4151
Acceder
id CONICETDig_3f2a56869e4f252e5138565f968a8471
oai_identifier_str oai:ri.conicet.gov.ar:11336/4151
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvantRivas Aravena, AndreaFuentes, JazminCartagena, JulioBrito, TaniaPoggio, Thelma Veronicala Torre, Jose LeonardoMendoza, HegalyGonzalez Nilo, Fernando DaniloSandino, Ana MaríaEugenio, Emilio OsvaldoNanoparticlesAdjuvantVaccineAtlantic SalmonSalmon AlphavirusIsavhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.Fil: Rivas Aravena, Andrea. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile. Comision Chilena de Energía Nuclear. Departamento de Aplicaciones Nucleares; ChileFil: Fuentes, Jazmin. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; ChileFil: Cartagena, Julio. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; ChileFil: Brito, Tania. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; ChileFil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina. Tecnovax; ArgentinaFil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina. Tecnovax; ArgentinaFil: Mendoza, Hegaly. Universidad Andr és Bello. Center for Bioinformatics and Integrative Biology; ChileFil: Gonzalez Nilo, Fernando Danilo. Universidad Andr és Bello. Center for Bioinformatics and Integrative Biology; ChileFil: Sandino, Ana María. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; ChileFil: Eugenio, Emilio Osvaldo. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; ChileElsevier2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4151Rivas Aravena, Andrea; Fuentes, Jazmin; Cartagena, Julio; Brito, Tania; Poggio, Thelma Veronica; et al.; Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant; Elsevier; Fish & Shellfish Immunology; 45; 1; 7-2015; 157-1661050-4648enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1050464815001424info:eu-repo/semantics/altIdentifier/issn/http://dx.doi.org/10.1016/j.fsi.2015.03.033info:eu-repo/semantics/altIdentifier/issn/1050-4648info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:29Zoai:ri.conicet.gov.ar:11336/4151instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:29.874CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
title Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
spellingShingle Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
Rivas Aravena, Andrea
Nanoparticles
Adjuvant
Vaccine
Atlantic Salmon
Salmon Alphavirus
Isav
title_short Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
title_full Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
title_fullStr Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
title_full_unstemmed Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
title_sort Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant
dc.creator.none.fl_str_mv Rivas Aravena, Andrea
Fuentes, Jazmin
Cartagena, Julio
Brito, Tania
Poggio, Thelma Veronica
la Torre, Jose Leonardo
Mendoza, Hegaly
Gonzalez Nilo, Fernando Danilo
Sandino, Ana María
Eugenio, Emilio Osvaldo
author Rivas Aravena, Andrea
author_facet Rivas Aravena, Andrea
Fuentes, Jazmin
Cartagena, Julio
Brito, Tania
Poggio, Thelma Veronica
la Torre, Jose Leonardo
Mendoza, Hegaly
Gonzalez Nilo, Fernando Danilo
Sandino, Ana María
Eugenio, Emilio Osvaldo
author_role author
author2 Fuentes, Jazmin
Cartagena, Julio
Brito, Tania
Poggio, Thelma Veronica
la Torre, Jose Leonardo
Mendoza, Hegaly
Gonzalez Nilo, Fernando Danilo
Sandino, Ana María
Eugenio, Emilio Osvaldo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanoparticles
Adjuvant
Vaccine
Atlantic Salmon
Salmon Alphavirus
Isav
topic Nanoparticles
Adjuvant
Vaccine
Atlantic Salmon
Salmon Alphavirus
Isav
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.
Fil: Rivas Aravena, Andrea. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile. Comision Chilena de Energía Nuclear. Departamento de Aplicaciones Nucleares; Chile
Fil: Fuentes, Jazmin. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Cartagena, Julio. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Brito, Tania. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Poggio, Thelma Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina. Tecnovax; Argentina
Fil: la Torre, Jose Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina. Tecnovax; Argentina
Fil: Mendoza, Hegaly. Universidad Andr és Bello. Center for Bioinformatics and Integrative Biology; Chile
Fil: Gonzalez Nilo, Fernando Danilo. Universidad Andr és Bello. Center for Bioinformatics and Integrative Biology; Chile
Fil: Sandino, Ana María. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
Fil: Eugenio, Emilio Osvaldo. Universidad de Santiago. Facultad de Química y Biología. Centro de Biotecnología Acuícola. Laboratorio de Virología; Chile
description Adjuvants used in vaccine aquaculture are frequently harmful for the fish, causing melanosis, granulomas and kidney damage. Along with that, vaccines are mostly administered by injection, causing pain and stress to the fish. We used the DNA coding for the replicase of alphavirus as adjuvant (Ad) of a vaccine against ISAV. The Ad and an inactivated ISAV (V) were loaded in chitosan nanoparticles (NPs) to be administered orally to Atlantic salmon. NP-Ad was able to deliver the DNA ex vivo and in vivo. Oral administration of the NPs stimulated the expression of immune molecules, but did not stimulate the humoral response. Although the vaccination with NP-V results in a modest protection of fish against ISAV, NP-V administered together with NP-Ad caused a protection of 77%. Therefore, the DNA coding for the replicase of alphavirus could be administered orally and can potentiate the immuneprotection of a virine against infection.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4151
Rivas Aravena, Andrea; Fuentes, Jazmin; Cartagena, Julio; Brito, Tania; Poggio, Thelma Veronica; et al.; Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant; Elsevier; Fish & Shellfish Immunology; 45; 1; 7-2015; 157-166
1050-4648
url http://hdl.handle.net/11336/4151
identifier_str_mv Rivas Aravena, Andrea; Fuentes, Jazmin; Cartagena, Julio; Brito, Tania; Poggio, Thelma Veronica; et al.; Development of a nanoparticle-based oral vaccine for Atlantic salmon against ISAV using an alphavirus replicon as adjuvant; Elsevier; Fish & Shellfish Immunology; 45; 1; 7-2015; 157-166
1050-4648
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1050464815001424
info:eu-repo/semantics/altIdentifier/issn/http://dx.doi.org/10.1016/j.fsi.2015.03.033
info:eu-repo/semantics/altIdentifier/issn/1050-4648
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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