Development of a scalable recombinant system for cyclic beta-1,2-glucans production

Autores
Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; Ciocchini, Andres Eduardo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields.
Fil: Guidolin, Leticia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Caillava, Ana Josefina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Comerci, Diego José. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ciocchini, Andres Eduardo. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
CYCLIC BETA-1,2-GLUCANS
CYCLOSOPHORAOSES
CYCLODEXTRIN
OLIGOSACCHARIDES
DRUG SOLUBILIZATION
NANOMATERIAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260982
Acceder
id CONICETDig_3ed2e1ed4bffae9841706d597e5a82c3
oai_identifier_str oai:ri.conicet.gov.ar:11336/260982
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a scalable recombinant system for cyclic beta-1,2-glucans productionGuidolin, Leticia SoledadCaillava, Ana JosefinaLandoni, MalenaCouto, Alicia SusanaComerci, Diego JoséCiocchini, Andres EduardoCYCLIC BETA-1,2-GLUCANSCYCLOSOPHORAOSESCYCLODEXTRINOLIGOSACCHARIDESDRUG SOLUBILIZATIONNANOMATERIALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields.Fil: Guidolin, Leticia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Caillava, Ana Josefina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Comerci, Diego José. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Ciocchini, Andres Eduardo. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaBioMed Central2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260982Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; et al.; Development of a scalable recombinant system for cyclic beta-1,2-glucans production; BioMed Central; Microbial Cell Factories; 23; 1; 5-2024; 1-111475-2859CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-024-02407-zinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-024-02407-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:45:04Zoai:ri.conicet.gov.ar:11336/260982instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:45:05.205CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a scalable recombinant system for cyclic beta-1,2-glucans production
title Development of a scalable recombinant system for cyclic beta-1,2-glucans production
spellingShingle Development of a scalable recombinant system for cyclic beta-1,2-glucans production
Guidolin, Leticia Soledad
CYCLIC BETA-1,2-GLUCANS
CYCLOSOPHORAOSES
CYCLODEXTRIN
OLIGOSACCHARIDES
DRUG SOLUBILIZATION
NANOMATERIAL
title_short Development of a scalable recombinant system for cyclic beta-1,2-glucans production
title_full Development of a scalable recombinant system for cyclic beta-1,2-glucans production
title_fullStr Development of a scalable recombinant system for cyclic beta-1,2-glucans production
title_full_unstemmed Development of a scalable recombinant system for cyclic beta-1,2-glucans production
title_sort Development of a scalable recombinant system for cyclic beta-1,2-glucans production
dc.creator.none.fl_str_mv Guidolin, Leticia Soledad
Caillava, Ana Josefina
Landoni, Malena
Couto, Alicia Susana
Comerci, Diego José
Ciocchini, Andres Eduardo
author Guidolin, Leticia Soledad
author_facet Guidolin, Leticia Soledad
Caillava, Ana Josefina
Landoni, Malena
Couto, Alicia Susana
Comerci, Diego José
Ciocchini, Andres Eduardo
author_role author
author2 Caillava, Ana Josefina
Landoni, Malena
Couto, Alicia Susana
Comerci, Diego José
Ciocchini, Andres Eduardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CYCLIC BETA-1,2-GLUCANS
CYCLOSOPHORAOSES
CYCLODEXTRIN
OLIGOSACCHARIDES
DRUG SOLUBILIZATION
NANOMATERIAL
topic CYCLIC BETA-1,2-GLUCANS
CYCLOSOPHORAOSES
CYCLODEXTRIN
OLIGOSACCHARIDES
DRUG SOLUBILIZATION
NANOMATERIAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields.
Fil: Guidolin, Leticia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Caillava, Ana Josefina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Comerci, Diego José. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ciocchini, Andres Eduardo. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
description Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields.
publishDate 2024
dc.date.none.fl_str_mv 2024-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260982
Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; et al.; Development of a scalable recombinant system for cyclic beta-1,2-glucans production; BioMed Central; Microbial Cell Factories; 23; 1; 5-2024; 1-11
1475-2859
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260982
identifier_str_mv Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; et al.; Development of a scalable recombinant system for cyclic beta-1,2-glucans production; BioMed Central; Microbial Cell Factories; 23; 1; 5-2024; 1-11
1475-2859
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-024-02407-z
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-024-02407-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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