Development of a scalable recombinant system for cyclic beta-1,2-glucans production
- Autores
- Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; Ciocchini, Andres Eduardo
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields.
Fil: Guidolin, Leticia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Caillava, Ana Josefina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Comerci, Diego José. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Ciocchini, Andres Eduardo. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina - Materia
-
CYCLIC BETA-1,2-GLUCANS
CYCLOSOPHORAOSES
CYCLODEXTRIN
OLIGOSACCHARIDES
DRUG SOLUBILIZATION
NANOMATERIAL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/260982
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Development of a scalable recombinant system for cyclic beta-1,2-glucans productionGuidolin, Leticia SoledadCaillava, Ana JosefinaLandoni, MalenaCouto, Alicia SusanaComerci, Diego JoséCiocchini, Andres EduardoCYCLIC BETA-1,2-GLUCANSCYCLOSOPHORAOSESCYCLODEXTRINOLIGOSACCHARIDESDRUG SOLUBILIZATIONNANOMATERIALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields.Fil: Guidolin, Leticia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Caillava, Ana Josefina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Comerci, Diego José. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Ciocchini, Andres Eduardo. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; ArgentinaBioMed Central2024-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260982Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; et al.; Development of a scalable recombinant system for cyclic beta-1,2-glucans production; BioMed Central; Microbial Cell Factories; 23; 1; 5-2024; 1-111475-2859CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-024-02407-zinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-024-02407-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:49Zoai:ri.conicet.gov.ar:11336/260982instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:49.81CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production |
title |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production |
spellingShingle |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production Guidolin, Leticia Soledad CYCLIC BETA-1,2-GLUCANS CYCLOSOPHORAOSES CYCLODEXTRIN OLIGOSACCHARIDES DRUG SOLUBILIZATION NANOMATERIAL |
title_short |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production |
title_full |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production |
title_fullStr |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production |
title_full_unstemmed |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production |
title_sort |
Development of a scalable recombinant system for cyclic beta-1,2-glucans production |
dc.creator.none.fl_str_mv |
Guidolin, Leticia Soledad Caillava, Ana Josefina Landoni, Malena Couto, Alicia Susana Comerci, Diego José Ciocchini, Andres Eduardo |
author |
Guidolin, Leticia Soledad |
author_facet |
Guidolin, Leticia Soledad Caillava, Ana Josefina Landoni, Malena Couto, Alicia Susana Comerci, Diego José Ciocchini, Andres Eduardo |
author_role |
author |
author2 |
Caillava, Ana Josefina Landoni, Malena Couto, Alicia Susana Comerci, Diego José Ciocchini, Andres Eduardo |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CYCLIC BETA-1,2-GLUCANS CYCLOSOPHORAOSES CYCLODEXTRIN OLIGOSACCHARIDES DRUG SOLUBILIZATION NANOMATERIAL |
topic |
CYCLIC BETA-1,2-GLUCANS CYCLOSOPHORAOSES CYCLODEXTRIN OLIGOSACCHARIDES DRUG SOLUBILIZATION NANOMATERIAL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields. Fil: Guidolin, Leticia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Caillava, Ana Josefina. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Landoni, Malena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Comerci, Diego José. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Ciocchini, Andres Eduardo. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina |
description |
Background Cyclic β-1,2-glucans (CβG) are bacterial cyclic homopolysaccharides with interesting biotechnologicalapplications. These ring-shaped molecules have a hydrophilic surface that confers high solubility and a hydrophobiccavity able to include poorly soluble molecules. Several studies demonstrate that CβG and many derivatives canbe applied in drug solubilization and stabilization, enantiomer separation, catalysis, synthesis of nanomaterials andeven as immunomodulators, suggesting these molecules have great potential for their industrial and commercialexploitation. Nowadays, there is no method to produce CβG by chemical synthesis and bacteria that synthesize themare slow-growing or even pathogenic, which makes the scaling up of the process difficult and expensive. Therefore,scalable production and purification methods are needed to afford the demand and expand the repertoire ofapplications of CβG.Results We present the production of CβG in specially designed E. coli strains by means of the deletion of intrinsicpolysaccharide biosynthetic genes and the heterologous expression of enzymes involved in CβG synthesis, transportand succinilation. These strains produce different types of CβG: unsubstituted CβG, anionic CβG and CβG of high size.Unsubstituted CβG with a degree of polymerization of 17 to 24 glucoses were produced and secreted to the culturemedium by one of the strains. Through high cell density culture (HCDC) of that strain we were able to produce 4,5 gof pure unsubstituted CβG /L in culture medium within 48 h culture.Conclusions We have developed a new recombinant bacterial system for the synthesis of cyclic β-1,2-glucans,expanding the use of bacteria as a platform for the production of new polysaccharides with biotechnologicalapplications. This new approach allowed us to produce CβG in E. coli with high yields and the highest volumetricproductivity reported to date. We expect this new highly scalable system facilitates CβG availability for furtherresearch and the widespread use of these promising molecules across many application fields. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/260982 Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; et al.; Development of a scalable recombinant system for cyclic beta-1,2-glucans production; BioMed Central; Microbial Cell Factories; 23; 1; 5-2024; 1-11 1475-2859 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/260982 |
identifier_str_mv |
Guidolin, Leticia Soledad; Caillava, Ana Josefina; Landoni, Malena; Couto, Alicia Susana; Comerci, Diego José; et al.; Development of a scalable recombinant system for cyclic beta-1,2-glucans production; BioMed Central; Microbial Cell Factories; 23; 1; 5-2024; 1-11 1475-2859 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-024-02407-z info:eu-repo/semantics/altIdentifier/doi/10.1186/s12934-024-02407-z |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614079710232576 |
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13.070432 |