Studying ion transport dynamics in electrochemical measurements of lateral flow assays

Autores
Le Brun, Grégoire; Calucho, Enric; Hauwaert, Margo; Moumneh, Ramy; Maroli, Gabriel; Yunus, Sami; Rosati, Giulio; Álvarez Diduk, Ruslan; Piper, Andrew; Merkoçi, Arben; Raskin, Jean Pierre
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lateral Flow Assays (LFAs) are among the most widely used biosensors. Conventional colorimetric LFAs yield binary, non-quantifiable results. Electrochemical LFAs (eLFAs) aim to overcome this limitation. However, the reported disadvantage of extreme sensitivity to experimental conditions poses a significant challenge in the development of new eLFAs. In this work, impedimetric measurements were performed to probe the impact of experimental factors such as electrolyte conductivity/ionic strength on the eLFA outcomes. The time-dependent evolution of non-Faradaic impedance measurements coupled with flow velocity computations were employed to investigate ion transport across different LFA configurations and buffer compositions. The analysis of the Péclet number, a characteristic dimensionless parameter of flow dynamics within the LFA, revealed the coexistence of diffusive and convective ionic transport regimes over the LFA operational time. In addition, our findings underscore the critical role of absorbent pad dead volume adjustments in governing the capacity to maintain sample flow within the membrane over extended durations. These electro-fluidics phenomena are essential considerations for conducting electrochemical measurements within LFAs. Overall, this study offers insights into key design parameters for the integration of electrochemistry into LFAs.
Fil: Le Brun, Grégoire. Université Catholique de Louvain; Bélgica
Fil: Calucho, Enric. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España
Fil: Hauwaert, Margo. Université Catholique de Louvain; Bélgica
Fil: Moumneh, Ramy. Université Catholique de Louvain; Bélgica
Fil: Maroli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España
Fil: Yunus, Sami. Université Catholique de Louvain; Bélgica
Fil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; España
Fil: Álvarez Diduk, Ruslan. Institut Català de Nanociència I Nanotecnologia; España
Fil: Piper, Andrew. Institut Català de Nanociència I Nanotecnologia; España
Fil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; España
Fil: Raskin, Jean Pierre. Université Catholique de Louvain; Bélgica
Materia
LATERAL-FLOW
MICROFLUIDICS
ELECTROCHEMISTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263134
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/263134
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Studying ion transport dynamics in electrochemical measurements of lateral flow assaysLe Brun, GrégoireCalucho, EnricHauwaert, MargoMoumneh, RamyMaroli, GabrielYunus, SamiRosati, GiulioÁlvarez Diduk, RuslanPiper, AndrewMerkoçi, ArbenRaskin, Jean PierreLATERAL-FLOWMICROFLUIDICSELECTROCHEMISTRYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Lateral Flow Assays (LFAs) are among the most widely used biosensors. Conventional colorimetric LFAs yield binary, non-quantifiable results. Electrochemical LFAs (eLFAs) aim to overcome this limitation. However, the reported disadvantage of extreme sensitivity to experimental conditions poses a significant challenge in the development of new eLFAs. In this work, impedimetric measurements were performed to probe the impact of experimental factors such as electrolyte conductivity/ionic strength on the eLFA outcomes. The time-dependent evolution of non-Faradaic impedance measurements coupled with flow velocity computations were employed to investigate ion transport across different LFA configurations and buffer compositions. The analysis of the Péclet number, a characteristic dimensionless parameter of flow dynamics within the LFA, revealed the coexistence of diffusive and convective ionic transport regimes over the LFA operational time. In addition, our findings underscore the critical role of absorbent pad dead volume adjustments in governing the capacity to maintain sample flow within the membrane over extended durations. These electro-fluidics phenomena are essential considerations for conducting electrochemical measurements within LFAs. Overall, this study offers insights into key design parameters for the integration of electrochemistry into LFAs.Fil: Le Brun, Grégoire. Université Catholique de Louvain; BélgicaFil: Calucho, Enric. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; EspañaFil: Hauwaert, Margo. Université Catholique de Louvain; BélgicaFil: Moumneh, Ramy. Université Catholique de Louvain; BélgicaFil: Maroli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; EspañaFil: Yunus, Sami. Université Catholique de Louvain; BélgicaFil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Álvarez Diduk, Ruslan. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Piper, Andrew. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; EspañaFil: Raskin, Jean Pierre. Université Catholique de Louvain; BélgicaElsevier Science SA2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263134Le Brun, Grégoire; Calucho, Enric; Hauwaert, Margo; Moumneh, Ramy; Maroli, Gabriel; et al.; Studying ion transport dynamics in electrochemical measurements of lateral flow assays; Elsevier Science SA; Journal of Electroanalytical Chemistry; 966; 118399; 8-2024; 1-91572-6657CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1572665724003771info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jelechem.2024.118399info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:07Zoai:ri.conicet.gov.ar:11336/263134instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:07.791CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title Studying ion transport dynamics in electrochemical measurements of lateral flow assays
spellingShingle Studying ion transport dynamics in electrochemical measurements of lateral flow assays
Le Brun, Grégoire
LATERAL-FLOW
MICROFLUIDICS
ELECTROCHEMISTRY
title_short Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_full Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_fullStr Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_full_unstemmed Studying ion transport dynamics in electrochemical measurements of lateral flow assays
title_sort Studying ion transport dynamics in electrochemical measurements of lateral flow assays
dc.creator.none.fl_str_mv Le Brun, Grégoire
Calucho, Enric
Hauwaert, Margo
Moumneh, Ramy
Maroli, Gabriel
Yunus, Sami
Rosati, Giulio
Álvarez Diduk, Ruslan
Piper, Andrew
Merkoçi, Arben
Raskin, Jean Pierre
author Le Brun, Grégoire
author_facet Le Brun, Grégoire
Calucho, Enric
Hauwaert, Margo
Moumneh, Ramy
Maroli, Gabriel
Yunus, Sami
Rosati, Giulio
Álvarez Diduk, Ruslan
Piper, Andrew
Merkoçi, Arben
Raskin, Jean Pierre
author_role author
author2 Calucho, Enric
Hauwaert, Margo
Moumneh, Ramy
Maroli, Gabriel
Yunus, Sami
Rosati, Giulio
Álvarez Diduk, Ruslan
Piper, Andrew
Merkoçi, Arben
Raskin, Jean Pierre
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LATERAL-FLOW
MICROFLUIDICS
ELECTROCHEMISTRY
topic LATERAL-FLOW
MICROFLUIDICS
ELECTROCHEMISTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lateral Flow Assays (LFAs) are among the most widely used biosensors. Conventional colorimetric LFAs yield binary, non-quantifiable results. Electrochemical LFAs (eLFAs) aim to overcome this limitation. However, the reported disadvantage of extreme sensitivity to experimental conditions poses a significant challenge in the development of new eLFAs. In this work, impedimetric measurements were performed to probe the impact of experimental factors such as electrolyte conductivity/ionic strength on the eLFA outcomes. The time-dependent evolution of non-Faradaic impedance measurements coupled with flow velocity computations were employed to investigate ion transport across different LFA configurations and buffer compositions. The analysis of the Péclet number, a characteristic dimensionless parameter of flow dynamics within the LFA, revealed the coexistence of diffusive and convective ionic transport regimes over the LFA operational time. In addition, our findings underscore the critical role of absorbent pad dead volume adjustments in governing the capacity to maintain sample flow within the membrane over extended durations. These electro-fluidics phenomena are essential considerations for conducting electrochemical measurements within LFAs. Overall, this study offers insights into key design parameters for the integration of electrochemistry into LFAs.
Fil: Le Brun, Grégoire. Université Catholique de Louvain; Bélgica
Fil: Calucho, Enric. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España
Fil: Hauwaert, Margo. Université Catholique de Louvain; Bélgica
Fil: Moumneh, Ramy. Université Catholique de Louvain; Bélgica
Fil: Maroli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España
Fil: Yunus, Sami. Université Catholique de Louvain; Bélgica
Fil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; España
Fil: Álvarez Diduk, Ruslan. Institut Català de Nanociència I Nanotecnologia; España
Fil: Piper, Andrew. Institut Català de Nanociència I Nanotecnologia; España
Fil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; España
Fil: Raskin, Jean Pierre. Université Catholique de Louvain; Bélgica
description Lateral Flow Assays (LFAs) are among the most widely used biosensors. Conventional colorimetric LFAs yield binary, non-quantifiable results. Electrochemical LFAs (eLFAs) aim to overcome this limitation. However, the reported disadvantage of extreme sensitivity to experimental conditions poses a significant challenge in the development of new eLFAs. In this work, impedimetric measurements were performed to probe the impact of experimental factors such as electrolyte conductivity/ionic strength on the eLFA outcomes. The time-dependent evolution of non-Faradaic impedance measurements coupled with flow velocity computations were employed to investigate ion transport across different LFA configurations and buffer compositions. The analysis of the Péclet number, a characteristic dimensionless parameter of flow dynamics within the LFA, revealed the coexistence of diffusive and convective ionic transport regimes over the LFA operational time. In addition, our findings underscore the critical role of absorbent pad dead volume adjustments in governing the capacity to maintain sample flow within the membrane over extended durations. These electro-fluidics phenomena are essential considerations for conducting electrochemical measurements within LFAs. Overall, this study offers insights into key design parameters for the integration of electrochemistry into LFAs.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263134
Le Brun, Grégoire; Calucho, Enric; Hauwaert, Margo; Moumneh, Ramy; Maroli, Gabriel; et al.; Studying ion transport dynamics in electrochemical measurements of lateral flow assays; Elsevier Science SA; Journal of Electroanalytical Chemistry; 966; 118399; 8-2024; 1-9
1572-6657
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263134
identifier_str_mv Le Brun, Grégoire; Calucho, Enric; Hauwaert, Margo; Moumneh, Ramy; Maroli, Gabriel; et al.; Studying ion transport dynamics in electrochemical measurements of lateral flow assays; Elsevier Science SA; Journal of Electroanalytical Chemistry; 966; 118399; 8-2024; 1-9
1572-6657
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1572665724003771
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jelechem.2024.118399
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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