Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights

Autores
Cook, Kimberly L.; Garret, Victoria; Layton, Alice C.; Dionisi, Hebe Monica; Sayler, Gary S.
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microorganisms will be an integral part of biologically based waste processing systems used for water purification or nutrient recycling on space flights.  Establishment of these systems with a defined group of microorganisms will provide a standardized means for conferring specific properties to the system.  Phylogenetic analysis of 16S rDNA sequences from a clonal library of organisms from a graywater-degrading rhizosphere community suggested that members of the Cytophagales and Proteobacter phylogenetic groups dominated. A clonal library of organisms from an industrial wastewater treatment plant (WWTP) was more diverse and consisted of organisms from more phylogenetic groups. This analysis provided the basis for selection of organisms for use in a defined, constructed community and for selection of a source of an undefined, complex microbial inoculum. The constructed community and the sludge inoculum were inoculated into a model plant rhizosphere waste processing system to evaluate survival. Based on plant, microbiological and molecular biological measures, it appeared that both inoculated communities were able to become established and persist in this model wastewater processing system.
Fil: Cook, Kimberly L.. University of Tennessee; Estados Unidos
Fil: Garret, Victoria. University of Tennessee; Estados Unidos
Fil: Layton, Alice C.. University of Tennessee; Estados Unidos
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina
Fil: Sayler, Gary S.. University of Tennessee; Estados Unidos
Materia
MICROBIAL INOCULA
GRAYWATER WASTE PROCESSING SYSTEMS
LONG-TERM SPACE FLIGHTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103675
Acceder
id CONICETDig_3d88074179d1cd0a158707afaf1ec822
oai_identifier_str oai:ri.conicet.gov.ar:11336/103675
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space FlightsCook, Kimberly L.Garret, VictoriaLayton, Alice C.Dionisi, Hebe MonicaSayler, Gary S.MICROBIAL INOCULAGRAYWATER WASTE PROCESSING SYSTEMSLONG-TERM SPACE FLIGHTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microorganisms will be an integral part of biologically based waste processing systems used for water purification or nutrient recycling on space flights.  Establishment of these systems with a defined group of microorganisms will provide a standardized means for conferring specific properties to the system.  Phylogenetic analysis of 16S rDNA sequences from a clonal library of organisms from a graywater-degrading rhizosphere community suggested that members of the Cytophagales and Proteobacter phylogenetic groups dominated. A clonal library of organisms from an industrial wastewater treatment plant (WWTP) was more diverse and consisted of organisms from more phylogenetic groups. This analysis provided the basis for selection of organisms for use in a defined, constructed community and for selection of a source of an undefined, complex microbial inoculum. The constructed community and the sludge inoculum were inoculated into a model plant rhizosphere waste processing system to evaluate survival. Based on plant, microbiological and molecular biological measures, it appeared that both inoculated communities were able to become established and persist in this model wastewater processing system.Fil: Cook, Kimberly L.. University of Tennessee; Estados UnidosFil: Garret, Victoria. University of Tennessee; Estados UnidosFil: Layton, Alice C.. University of Tennessee; Estados UnidosFil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; ArgentinaFil: Sayler, Gary S.. University of Tennessee; Estados UnidosSAE International2003-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103675Cook, Kimberly L.; Garret, Victoria; Layton, Alice C.; Dionisi, Hebe Monica; Sayler, Gary S.; Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights; SAE International; Sae Technical Papers; 2003; 7-2003; 1-110148-71912688-3627CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4271/2003-01-2512info:eu-repo/semantics/altIdentifier/url/https://saemobilus.sae.org/content/2003-01-2512/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T09:08:54Zoai:ri.conicet.gov.ar:11336/103675instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 09:08:54.954CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
title Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
spellingShingle Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
Cook, Kimberly L.
MICROBIAL INOCULA
GRAYWATER WASTE PROCESSING SYSTEMS
LONG-TERM SPACE FLIGHTS
title_short Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
title_full Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
title_fullStr Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
title_full_unstemmed Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
title_sort Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights
dc.creator.none.fl_str_mv Cook, Kimberly L.
Garret, Victoria
Layton, Alice C.
Dionisi, Hebe Monica
Sayler, Gary S.
author Cook, Kimberly L.
author_facet Cook, Kimberly L.
Garret, Victoria
Layton, Alice C.
Dionisi, Hebe Monica
Sayler, Gary S.
author_role author
author2 Garret, Victoria
Layton, Alice C.
Dionisi, Hebe Monica
Sayler, Gary S.
author2_role author
author
author
author
dc.subject.none.fl_str_mv MICROBIAL INOCULA
GRAYWATER WASTE PROCESSING SYSTEMS
LONG-TERM SPACE FLIGHTS
topic MICROBIAL INOCULA
GRAYWATER WASTE PROCESSING SYSTEMS
LONG-TERM SPACE FLIGHTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microorganisms will be an integral part of biologically based waste processing systems used for water purification or nutrient recycling on space flights.  Establishment of these systems with a defined group of microorganisms will provide a standardized means for conferring specific properties to the system.  Phylogenetic analysis of 16S rDNA sequences from a clonal library of organisms from a graywater-degrading rhizosphere community suggested that members of the Cytophagales and Proteobacter phylogenetic groups dominated. A clonal library of organisms from an industrial wastewater treatment plant (WWTP) was more diverse and consisted of organisms from more phylogenetic groups. This analysis provided the basis for selection of organisms for use in a defined, constructed community and for selection of a source of an undefined, complex microbial inoculum. The constructed community and the sludge inoculum were inoculated into a model plant rhizosphere waste processing system to evaluate survival. Based on plant, microbiological and molecular biological measures, it appeared that both inoculated communities were able to become established and persist in this model wastewater processing system.
Fil: Cook, Kimberly L.. University of Tennessee; Estados Unidos
Fil: Garret, Victoria. University of Tennessee; Estados Unidos
Fil: Layton, Alice C.. University of Tennessee; Estados Unidos
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina
Fil: Sayler, Gary S.. University of Tennessee; Estados Unidos
description Microorganisms will be an integral part of biologically based waste processing systems used for water purification or nutrient recycling on space flights.  Establishment of these systems with a defined group of microorganisms will provide a standardized means for conferring specific properties to the system.  Phylogenetic analysis of 16S rDNA sequences from a clonal library of organisms from a graywater-degrading rhizosphere community suggested that members of the Cytophagales and Proteobacter phylogenetic groups dominated. A clonal library of organisms from an industrial wastewater treatment plant (WWTP) was more diverse and consisted of organisms from more phylogenetic groups. This analysis provided the basis for selection of organisms for use in a defined, constructed community and for selection of a source of an undefined, complex microbial inoculum. The constructed community and the sludge inoculum were inoculated into a model plant rhizosphere waste processing system to evaluate survival. Based on plant, microbiological and molecular biological measures, it appeared that both inoculated communities were able to become established and persist in this model wastewater processing system.
publishDate 2003
dc.date.none.fl_str_mv 2003-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103675
Cook, Kimberly L.; Garret, Victoria; Layton, Alice C.; Dionisi, Hebe Monica; Sayler, Gary S.; Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights; SAE International; Sae Technical Papers; 2003; 7-2003; 1-11
0148-7191
2688-3627
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103675
identifier_str_mv Cook, Kimberly L.; Garret, Victoria; Layton, Alice C.; Dionisi, Hebe Monica; Sayler, Gary S.; Development and Molecular Characterization of Microbial Inocula for Initiation of Graywater Waste Processing Systems on Long-Term Space Flights; SAE International; Sae Technical Papers; 2003; 7-2003; 1-11
0148-7191
2688-3627
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4271/2003-01-2512
info:eu-repo/semantics/altIdentifier/url/https://saemobilus.sae.org/content/2003-01-2512/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAE International
publisher.none.fl_str_mv SAE International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1849873607340064768
score 13.011256

Publicaciones similares