Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus

Autores
Giorgio, Ernesto Martín; Díaz, Gabriela Verónica; Saparrat, Mario Carlos Nazareno; Zapata, Pedro Dario
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lignocellulosic residues generated by the agro-forestry industry are low-cost renewable biomass, rich in cellulose, and can be valorized by converting it into second-generation bioethanol. This work aimed to convert the rice straw, pine, and eucalyptus sawdust into fermentable sugars by applying an enzymatic cocktail from Irpex lacteus LBM 034 optimized for enhancing cellulase levels. The most influential medium compounds in the production of the fungal cellulolytic enzymes were identified by a Plackett Burman design. The most significant compounds were xylan, carboxymethylcellulose, and peptone. The physical conditions for fungal incubation were optimized using response surface methodology, with 32 °C and pH 5.5 without agitation identified as the optimal conditions. Under these conditions, the level of endo-β-1,4-glucanases in the cocktail was 2.6 times higher than the original. The optimized fungal cocktail was then applied to hydrolyze the polysaccharides of NaOH-pre-treated rice straw, pine, and eucalyptus sawdust into fermentable sugars. The use of this enzymatic cocktail enabled efficient hydrolysis of rice straw, yielding in 27.19% and 38.18% of hemicellulose and cellulose conversion, respectively. This bioconversion of cellulose and hemicellulose of renewable biomass into fermentable sugars represents a cost-effective bioprocess for applications such as the production of lignocellulosic bioethanol.
Fil: Giorgio, Ernesto Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Saparrat, Mario Carlos Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Materia
BIOPRODUCTOS
ENZIMAS
LIGNOCELULOSIC
BIOCONVERSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279980
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteusGiorgio, Ernesto MartínDíaz, Gabriela VerónicaSaparrat, Mario Carlos NazarenoZapata, Pedro DarioBIOPRODUCTOSENZIMASLIGNOCELULOSICBIOCONVERSIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Lignocellulosic residues generated by the agro-forestry industry are low-cost renewable biomass, rich in cellulose, and can be valorized by converting it into second-generation bioethanol. This work aimed to convert the rice straw, pine, and eucalyptus sawdust into fermentable sugars by applying an enzymatic cocktail from Irpex lacteus LBM 034 optimized for enhancing cellulase levels. The most influential medium compounds in the production of the fungal cellulolytic enzymes were identified by a Plackett Burman design. The most significant compounds were xylan, carboxymethylcellulose, and peptone. The physical conditions for fungal incubation were optimized using response surface methodology, with 32 °C and pH 5.5 without agitation identified as the optimal conditions. Under these conditions, the level of endo-β-1,4-glucanases in the cocktail was 2.6 times higher than the original. The optimized fungal cocktail was then applied to hydrolyze the polysaccharides of NaOH-pre-treated rice straw, pine, and eucalyptus sawdust into fermentable sugars. The use of this enzymatic cocktail enabled efficient hydrolysis of rice straw, yielding in 27.19% and 38.18% of hemicellulose and cellulose conversion, respectively. This bioconversion of cellulose and hemicellulose of renewable biomass into fermentable sugars represents a cost-effective bioprocess for applications such as the production of lignocellulosic bioethanol.Fil: Giorgio, Ernesto Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Saparrat, Mario Carlos Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaMushroom Research Foundation2025-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279980Giorgio, Ernesto Martín; Díaz, Gabriela Verónica; Saparrat, Mario Carlos Nazareno; Zapata, Pedro Dario; Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus; Mushroom Research Foundation; Asian Journal of Mycology; 8; 2; 10-2025; 76-912651-1339CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://asianjournalofmycology.org/pdf/AJOM_8_2_6.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.5943/ajom/8/2/6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:41:04Zoai:ri.conicet.gov.ar:11336/279980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:41:04.335CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
title Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
spellingShingle Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
Giorgio, Ernesto Martín
BIOPRODUCTOS
ENZIMAS
LIGNOCELULOSIC
BIOCONVERSION
title_short Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
title_full Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
title_fullStr Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
title_full_unstemmed Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
title_sort Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus
dc.creator.none.fl_str_mv Giorgio, Ernesto Martín
Díaz, Gabriela Verónica
Saparrat, Mario Carlos Nazareno
Zapata, Pedro Dario
author Giorgio, Ernesto Martín
author_facet Giorgio, Ernesto Martín
Díaz, Gabriela Verónica
Saparrat, Mario Carlos Nazareno
Zapata, Pedro Dario
author_role author
author2 Díaz, Gabriela Verónica
Saparrat, Mario Carlos Nazareno
Zapata, Pedro Dario
author2_role author
author
author
dc.subject.none.fl_str_mv BIOPRODUCTOS
ENZIMAS
LIGNOCELULOSIC
BIOCONVERSION
topic BIOPRODUCTOS
ENZIMAS
LIGNOCELULOSIC
BIOCONVERSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Lignocellulosic residues generated by the agro-forestry industry are low-cost renewable biomass, rich in cellulose, and can be valorized by converting it into second-generation bioethanol. This work aimed to convert the rice straw, pine, and eucalyptus sawdust into fermentable sugars by applying an enzymatic cocktail from Irpex lacteus LBM 034 optimized for enhancing cellulase levels. The most influential medium compounds in the production of the fungal cellulolytic enzymes were identified by a Plackett Burman design. The most significant compounds were xylan, carboxymethylcellulose, and peptone. The physical conditions for fungal incubation were optimized using response surface methodology, with 32 °C and pH 5.5 without agitation identified as the optimal conditions. Under these conditions, the level of endo-β-1,4-glucanases in the cocktail was 2.6 times higher than the original. The optimized fungal cocktail was then applied to hydrolyze the polysaccharides of NaOH-pre-treated rice straw, pine, and eucalyptus sawdust into fermentable sugars. The use of this enzymatic cocktail enabled efficient hydrolysis of rice straw, yielding in 27.19% and 38.18% of hemicellulose and cellulose conversion, respectively. This bioconversion of cellulose and hemicellulose of renewable biomass into fermentable sugars represents a cost-effective bioprocess for applications such as the production of lignocellulosic bioethanol.
Fil: Giorgio, Ernesto Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Díaz, Gabriela Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Saparrat, Mario Carlos Nazareno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
description Lignocellulosic residues generated by the agro-forestry industry are low-cost renewable biomass, rich in cellulose, and can be valorized by converting it into second-generation bioethanol. This work aimed to convert the rice straw, pine, and eucalyptus sawdust into fermentable sugars by applying an enzymatic cocktail from Irpex lacteus LBM 034 optimized for enhancing cellulase levels. The most influential medium compounds in the production of the fungal cellulolytic enzymes were identified by a Plackett Burman design. The most significant compounds were xylan, carboxymethylcellulose, and peptone. The physical conditions for fungal incubation were optimized using response surface methodology, with 32 °C and pH 5.5 without agitation identified as the optimal conditions. Under these conditions, the level of endo-β-1,4-glucanases in the cocktail was 2.6 times higher than the original. The optimized fungal cocktail was then applied to hydrolyze the polysaccharides of NaOH-pre-treated rice straw, pine, and eucalyptus sawdust into fermentable sugars. The use of this enzymatic cocktail enabled efficient hydrolysis of rice straw, yielding in 27.19% and 38.18% of hemicellulose and cellulose conversion, respectively. This bioconversion of cellulose and hemicellulose of renewable biomass into fermentable sugars represents a cost-effective bioprocess for applications such as the production of lignocellulosic bioethanol.
publishDate 2025
dc.date.none.fl_str_mv 2025-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279980
Giorgio, Ernesto Martín; Díaz, Gabriela Verónica; Saparrat, Mario Carlos Nazareno; Zapata, Pedro Dario; Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus; Mushroom Research Foundation; Asian Journal of Mycology; 8; 2; 10-2025; 76-91
2651-1339
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279980
identifier_str_mv Giorgio, Ernesto Martín; Díaz, Gabriela Verónica; Saparrat, Mario Carlos Nazareno; Zapata, Pedro Dario; Lignocellulosic bioconversion by an optimized enzymatic cocktail from Irpex lacteus; Mushroom Research Foundation; Asian Journal of Mycology; 8; 2; 10-2025; 76-91
2651-1339
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://asianjournalofmycology.org/pdf/AJOM_8_2_6.pdf
info:eu-repo/semantics/altIdentifier/doi/10.5943/ajom/8/2/6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mushroom Research Foundation
publisher.none.fl_str_mv Mushroom Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.11174

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