Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells

Autores
Meroni, Silvina Beatriz; Suburo, Angela Maria; Cigorraga, Selva Beatriz
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several cytokines have been involved in the regulation of Sertoli cell function. Further investigations are required to elucidate the role of interleukin-1beta (IL1beta) in Sertoli cell physiology. Twenty-day-old rat Sertoli cell cultures were used to investigate a possible role of IL1beta in the regulation of gamma-glutamyl transpeptidase (gammaGTP) and to elucidate the signaling pathway utilized by this cytokine. GammaGTP is a membrane-bound enzyme that has been involved in amino acid transport across the plasma membrane and in protection from oxidative stress through its importance in the regulation of glutathione levels. Previous studies suggested that IL1beta stimulates NO biosynthesis in other cell types. Therefore, we investigated whether IL1beta modified the level of nitrite, a stable metabolite of NO, in Sertoli cells. Dose-response curves to IL1beta for gammaGTP activity and nitrite production were observed. The increments observed in gammaGTP activity and nitrite production were partially and completely blocked by simultaneous treatment with the NO synthase inhibitor aminoguanidine. Treatment of Sertoli cell cultures with the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine resulted in an increase in gammaGTP activity. The presence of neural, endothelial, and inducible isoforms of NO synthase (NOS) was investigated by a immunohistochemical technique using specific antibodies. The 2 constitutive isoforms were present under basal conditions, and the inducible protein appeared in IL1beta-treated cultures. Finally, translocation of NF-kappaB p65 subunit to the nucleus in IL1beta-treated cultures was observed. These findings suggest that the action of IL1beta on Sertoli cell gammaGTP activity is partially mediated via activation of NF-kappaB and increments in iNOS and cellular production of NO.
Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Materia
Testis
Cytokines
Nitric Oxide Synthase
Nf-Kb
Signal Transduction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71776
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71776
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cellsMeroni, Silvina BeatrizSuburo, Angela MariaCigorraga, Selva BeatrizTestisCytokinesNitric Oxide SynthaseNf-KbSignal Transductionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Several cytokines have been involved in the regulation of Sertoli cell function. Further investigations are required to elucidate the role of interleukin-1beta (IL1beta) in Sertoli cell physiology. Twenty-day-old rat Sertoli cell cultures were used to investigate a possible role of IL1beta in the regulation of gamma-glutamyl transpeptidase (gammaGTP) and to elucidate the signaling pathway utilized by this cytokine. GammaGTP is a membrane-bound enzyme that has been involved in amino acid transport across the plasma membrane and in protection from oxidative stress through its importance in the regulation of glutathione levels. Previous studies suggested that IL1beta stimulates NO biosynthesis in other cell types. Therefore, we investigated whether IL1beta modified the level of nitrite, a stable metabolite of NO, in Sertoli cells. Dose-response curves to IL1beta for gammaGTP activity and nitrite production were observed. The increments observed in gammaGTP activity and nitrite production were partially and completely blocked by simultaneous treatment with the NO synthase inhibitor aminoguanidine. Treatment of Sertoli cell cultures with the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine resulted in an increase in gammaGTP activity. The presence of neural, endothelial, and inducible isoforms of NO synthase (NOS) was investigated by a immunohistochemical technique using specific antibodies. The 2 constitutive isoforms were present under basal conditions, and the inducible protein appeared in IL1beta-treated cultures. Finally, translocation of NF-kappaB p65 subunit to the nucleus in IL1beta-treated cultures was observed. These findings suggest that the action of IL1beta on Sertoli cell gammaGTP activity is partially mediated via activation of NF-kappaB and increments in iNOS and cellular production of NO.Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaFil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomédicas; ArgentinaFil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; ArgentinaAmerican Society of Andrology2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71776Meroni, Silvina Beatriz; Suburo, Angela Maria; Cigorraga, Selva Beatriz; Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells; American Society of Andrology; Journal of Andrology; 21; 6; 12-2000; 855-8610196-3635CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/j.1939-4640.2000.tb03416.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/j.1939-4640.2000.tb03416.x?sid=nlm%3Apubmedinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:45:21Zoai:ri.conicet.gov.ar:11336/71776instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:45:21.882CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
title Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
spellingShingle Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
Meroni, Silvina Beatriz
Testis
Cytokines
Nitric Oxide Synthase
Nf-Kb
Signal Transduction
title_short Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
title_full Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
title_fullStr Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
title_full_unstemmed Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
title_sort Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells
dc.creator.none.fl_str_mv Meroni, Silvina Beatriz
Suburo, Angela Maria
Cigorraga, Selva Beatriz
author Meroni, Silvina Beatriz
author_facet Meroni, Silvina Beatriz
Suburo, Angela Maria
Cigorraga, Selva Beatriz
author_role author
author2 Suburo, Angela Maria
Cigorraga, Selva Beatriz
author2_role author
author
dc.subject.none.fl_str_mv Testis
Cytokines
Nitric Oxide Synthase
Nf-Kb
Signal Transduction
topic Testis
Cytokines
Nitric Oxide Synthase
Nf-Kb
Signal Transduction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Several cytokines have been involved in the regulation of Sertoli cell function. Further investigations are required to elucidate the role of interleukin-1beta (IL1beta) in Sertoli cell physiology. Twenty-day-old rat Sertoli cell cultures were used to investigate a possible role of IL1beta in the regulation of gamma-glutamyl transpeptidase (gammaGTP) and to elucidate the signaling pathway utilized by this cytokine. GammaGTP is a membrane-bound enzyme that has been involved in amino acid transport across the plasma membrane and in protection from oxidative stress through its importance in the regulation of glutathione levels. Previous studies suggested that IL1beta stimulates NO biosynthesis in other cell types. Therefore, we investigated whether IL1beta modified the level of nitrite, a stable metabolite of NO, in Sertoli cells. Dose-response curves to IL1beta for gammaGTP activity and nitrite production were observed. The increments observed in gammaGTP activity and nitrite production were partially and completely blocked by simultaneous treatment with the NO synthase inhibitor aminoguanidine. Treatment of Sertoli cell cultures with the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine resulted in an increase in gammaGTP activity. The presence of neural, endothelial, and inducible isoforms of NO synthase (NOS) was investigated by a immunohistochemical technique using specific antibodies. The 2 constitutive isoforms were present under basal conditions, and the inducible protein appeared in IL1beta-treated cultures. Finally, translocation of NF-kappaB p65 subunit to the nucleus in IL1beta-treated cultures was observed. These findings suggest that the action of IL1beta on Sertoli cell gammaGTP activity is partially mediated via activation of NF-kappaB and increments in iNOS and cellular production of NO.
Fil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
Fil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomédicas; Argentina
Fil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina
description Several cytokines have been involved in the regulation of Sertoli cell function. Further investigations are required to elucidate the role of interleukin-1beta (IL1beta) in Sertoli cell physiology. Twenty-day-old rat Sertoli cell cultures were used to investigate a possible role of IL1beta in the regulation of gamma-glutamyl transpeptidase (gammaGTP) and to elucidate the signaling pathway utilized by this cytokine. GammaGTP is a membrane-bound enzyme that has been involved in amino acid transport across the plasma membrane and in protection from oxidative stress through its importance in the regulation of glutathione levels. Previous studies suggested that IL1beta stimulates NO biosynthesis in other cell types. Therefore, we investigated whether IL1beta modified the level of nitrite, a stable metabolite of NO, in Sertoli cells. Dose-response curves to IL1beta for gammaGTP activity and nitrite production were observed. The increments observed in gammaGTP activity and nitrite production were partially and completely blocked by simultaneous treatment with the NO synthase inhibitor aminoguanidine. Treatment of Sertoli cell cultures with the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine resulted in an increase in gammaGTP activity. The presence of neural, endothelial, and inducible isoforms of NO synthase (NOS) was investigated by a immunohistochemical technique using specific antibodies. The 2 constitutive isoforms were present under basal conditions, and the inducible protein appeared in IL1beta-treated cultures. Finally, translocation of NF-kappaB p65 subunit to the nucleus in IL1beta-treated cultures was observed. These findings suggest that the action of IL1beta on Sertoli cell gammaGTP activity is partially mediated via activation of NF-kappaB and increments in iNOS and cellular production of NO.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71776
Meroni, Silvina Beatriz; Suburo, Angela Maria; Cigorraga, Selva Beatriz; Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells; American Society of Andrology; Journal of Andrology; 21; 6; 12-2000; 855-861
0196-3635
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71776
identifier_str_mv Meroni, Silvina Beatriz; Suburo, Angela Maria; Cigorraga, Selva Beatriz; Interleukin-1ß regulates nitric oxide production and gamma-glutamyltranspeptidase activity in Sertoli cells; American Society of Andrology; Journal of Andrology; 21; 6; 12-2000; 855-861
0196-3635
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/j.1939-4640.2000.tb03416.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/j.1939-4640.2000.tb03416.x?sid=nlm%3Apubmed
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Andrology
publisher.none.fl_str_mv American Society of Andrology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606805585854464
score 13.000565

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