Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment

Autores
Bublik, Débora Rosa; Scolz, Massimiliano; Triolo, Gianluca; Monte, Martin; Schneider, Claudio
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
p21CIP1/WAF1 belongs to the CIP/KIP family of Cdk inhibitors, and its expression is tightly controlled during the cell cycle, mainly by transcriptional and post-translational mechanisms. Fine regulation of p21 CIP1/WAF1 levels is critical for cell cycle control and for cellular response to stress. In the present work, we describe a novel mechanism to modulate p21CIP1/WAF1 levels mediated by the human GTSE-1 (G 2 and S phase-expressed-1) protein. Our results provide evidence that hGTSE-1 protects p21CIP1/WAF1 from proteasome-dependent degradation as part of a functional complex containing the Hsp90-bindingTPR protein WISp39. We further show that the hGTSE-1 N-terminal portion is sufficient for p21 CIP1/WAF1 binding and stabilization. Finally, we demonstrate that hGTSE-1 mediated-p21CIP1/WAF1 stabilization is clearly involved in the ability of cells to counter-act cytotoxicity induced by the microtubule poison paclitaxel. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Fil: Bublik, Débora Rosa. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia
Fil: Scolz, Massimiliano. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia
Fil: Triolo, Gianluca. Genome Stability Laboratory; Italia
Fil: Monte, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia
Fil: Schneider, Claudio. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia. Università di Udine; Italia
Materia
GTSE
p21
taxol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66674
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66674
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatmentBublik, Débora RosaScolz, MassimilianoTriolo, GianlucaMonte, MartinSchneider, ClaudioGTSEp21taxolhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1p21CIP1/WAF1 belongs to the CIP/KIP family of Cdk inhibitors, and its expression is tightly controlled during the cell cycle, mainly by transcriptional and post-translational mechanisms. Fine regulation of p21 CIP1/WAF1 levels is critical for cell cycle control and for cellular response to stress. In the present work, we describe a novel mechanism to modulate p21CIP1/WAF1 levels mediated by the human GTSE-1 (G 2 and S phase-expressed-1) protein. Our results provide evidence that hGTSE-1 protects p21CIP1/WAF1 from proteasome-dependent degradation as part of a functional complex containing the Hsp90-bindingTPR protein WISp39. We further show that the hGTSE-1 N-terminal portion is sufficient for p21 CIP1/WAF1 binding and stabilization. Finally, we demonstrate that hGTSE-1 mediated-p21CIP1/WAF1 stabilization is clearly involved in the ability of cells to counter-act cytotoxicity induced by the microtubule poison paclitaxel. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.Fil: Bublik, Débora Rosa. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; ItaliaFil: Scolz, Massimiliano. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; ItaliaFil: Triolo, Gianluca. Genome Stability Laboratory; ItaliaFil: Monte, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; ItaliaFil: Schneider, Claudio. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia. Università di Udine; ItaliaAmerican Society for Biochemistry and Molecular Biology2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66674Bublik, Débora Rosa; Scolz, Massimiliano; Triolo, Gianluca; Monte, Martin; Schneider, Claudio; Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 285; 8; 2-2010; 5274-52810021-9258CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M109.045948info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/285/8/5274info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:51Zoai:ri.conicet.gov.ar:11336/66674instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:51.835CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
title Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
spellingShingle Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
Bublik, Débora Rosa
GTSE
p21
taxol
title_short Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
title_full Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
title_fullStr Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
title_full_unstemmed Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
title_sort Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment
dc.creator.none.fl_str_mv Bublik, Débora Rosa
Scolz, Massimiliano
Triolo, Gianluca
Monte, Martin
Schneider, Claudio
author Bublik, Débora Rosa
author_facet Bublik, Débora Rosa
Scolz, Massimiliano
Triolo, Gianluca
Monte, Martin
Schneider, Claudio
author_role author
author2 Scolz, Massimiliano
Triolo, Gianluca
Monte, Martin
Schneider, Claudio
author2_role author
author
author
author
dc.subject.none.fl_str_mv GTSE
p21
taxol
topic GTSE
p21
taxol
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv p21CIP1/WAF1 belongs to the CIP/KIP family of Cdk inhibitors, and its expression is tightly controlled during the cell cycle, mainly by transcriptional and post-translational mechanisms. Fine regulation of p21 CIP1/WAF1 levels is critical for cell cycle control and for cellular response to stress. In the present work, we describe a novel mechanism to modulate p21CIP1/WAF1 levels mediated by the human GTSE-1 (G 2 and S phase-expressed-1) protein. Our results provide evidence that hGTSE-1 protects p21CIP1/WAF1 from proteasome-dependent degradation as part of a functional complex containing the Hsp90-bindingTPR protein WISp39. We further show that the hGTSE-1 N-terminal portion is sufficient for p21 CIP1/WAF1 binding and stabilization. Finally, we demonstrate that hGTSE-1 mediated-p21CIP1/WAF1 stabilization is clearly involved in the ability of cells to counter-act cytotoxicity induced by the microtubule poison paclitaxel. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
Fil: Bublik, Débora Rosa. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia
Fil: Scolz, Massimiliano. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia
Fil: Triolo, Gianluca. Genome Stability Laboratory; Italia
Fil: Monte, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia
Fil: Schneider, Claudio. Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie ; Italia. Università di Udine; Italia
description p21CIP1/WAF1 belongs to the CIP/KIP family of Cdk inhibitors, and its expression is tightly controlled during the cell cycle, mainly by transcriptional and post-translational mechanisms. Fine regulation of p21 CIP1/WAF1 levels is critical for cell cycle control and for cellular response to stress. In the present work, we describe a novel mechanism to modulate p21CIP1/WAF1 levels mediated by the human GTSE-1 (G 2 and S phase-expressed-1) protein. Our results provide evidence that hGTSE-1 protects p21CIP1/WAF1 from proteasome-dependent degradation as part of a functional complex containing the Hsp90-bindingTPR protein WISp39. We further show that the hGTSE-1 N-terminal portion is sufficient for p21 CIP1/WAF1 binding and stabilization. Finally, we demonstrate that hGTSE-1 mediated-p21CIP1/WAF1 stabilization is clearly involved in the ability of cells to counter-act cytotoxicity induced by the microtubule poison paclitaxel. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66674
Bublik, Débora Rosa; Scolz, Massimiliano; Triolo, Gianluca; Monte, Martin; Schneider, Claudio; Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 285; 8; 2-2010; 5274-5281
0021-9258
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66674
identifier_str_mv Bublik, Débora Rosa; Scolz, Massimiliano; Triolo, Gianluca; Monte, Martin; Schneider, Claudio; Human GTSE-1 regulates p21CIP1/WAF1 stability conferring resistance to paclitaxel treatment; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 285; 8; 2-2010; 5274-5281
0021-9258
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M109.045948
info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/285/8/5274
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268758348922880
score 13.13397

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