Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina

Autores
Mooney, Ronnie; Rodgers, Kiri; Carnicelli, Sandro; Carnevale, Matías Emanuel; Farias, Maria Eugenia; Henriquez, Fiona L.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acanthamoeba spp. are widespread protists that feed on bacteria via phagocytosis. This predation pressure has led many bacteria to evolve strategies to resist and survive inside these protists. The impact of this is not well understood, but it may limit detection and allow survival in extreme environments. Three sites in the Puna salt plains, Catamarca province, Argentina, were sampled for Acanthamoeba spp., verified using PCR and Sanger sequencing. The intracellular microbiome was analysed with 16S rRNA gene sequencing and compared to the overall site microbiome. Acanthamoeba were found at all locations, and their intracellular microbiome was similar across samples but differed from the overall site microbiome. Pseudomonas spp., a clinically relevant genus, was most abundant in all isolates. This study suggests Acanthamoeba can protect bacteria, aiding their detection avoidance and survival in harsh conditions.
Fil: Mooney, Ronnie. University of Strathclyde; Reino Unido
Fil: Rodgers, Kiri. University Of The West Of Scotland; Reino Unido
Fil: Carnicelli, Sandro. University Of The West Of Scotland; Reino Unido
Fil: Carnevale, Matías Emanuel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Centro Regional de Energia y Ambiente Para El Desarrollo Sustentable. - Universidad Nacional de Catamarca. Centro Regional de Energia y Ambiente Para El Desarrollo Sustentable.; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Henriquez, Fiona L.. University of Strathclyde; Reino Unido
Materia
ACANTHAMOEBA
INTRACELLULAR
MICROBIOME
SYMBIOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261480
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, ArgentinaMooney, RonnieRodgers, KiriCarnicelli, SandroCarnevale, Matías EmanuelFarias, Maria EugeniaHenriquez, Fiona L.ACANTHAMOEBAINTRACELLULARMICROBIOMESYMBIOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acanthamoeba spp. are widespread protists that feed on bacteria via phagocytosis. This predation pressure has led many bacteria to evolve strategies to resist and survive inside these protists. The impact of this is not well understood, but it may limit detection and allow survival in extreme environments. Three sites in the Puna salt plains, Catamarca province, Argentina, were sampled for Acanthamoeba spp., verified using PCR and Sanger sequencing. The intracellular microbiome was analysed with 16S rRNA gene sequencing and compared to the overall site microbiome. Acanthamoeba were found at all locations, and their intracellular microbiome was similar across samples but differed from the overall site microbiome. Pseudomonas spp., a clinically relevant genus, was most abundant in all isolates. This study suggests Acanthamoeba can protect bacteria, aiding their detection avoidance and survival in harsh conditions.Fil: Mooney, Ronnie. University of Strathclyde; Reino UnidoFil: Rodgers, Kiri. University Of The West Of Scotland; Reino UnidoFil: Carnicelli, Sandro. University Of The West Of Scotland; Reino UnidoFil: Carnevale, Matías Emanuel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Centro Regional de Energia y Ambiente Para El Desarrollo Sustentable. - Universidad Nacional de Catamarca. Centro Regional de Energia y Ambiente Para El Desarrollo Sustentable.; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Henriquez, Fiona L.. University of Strathclyde; Reino UnidoJohn Wiley & Sons2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261480Mooney, Ronnie; Rodgers, Kiri; Carnicelli, Sandro; Carnevale, Matías Emanuel; Farias, Maria Eugenia; et al.; Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina; John Wiley & Sons; Environmental Microbiology Reports; 17; 1; 11-2024; 1-101758-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.70059info:eu-repo/semantics/altIdentifier/doi/10.1111/1758-2229.70059info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:59Zoai:ri.conicet.gov.ar:11336/261480instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:59.504CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
title Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
spellingShingle Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
Mooney, Ronnie
ACANTHAMOEBA
INTRACELLULAR
MICROBIOME
SYMBIOSIS
title_short Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
title_full Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
title_fullStr Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
title_full_unstemmed Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
title_sort Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina
dc.creator.none.fl_str_mv Mooney, Ronnie
Rodgers, Kiri
Carnicelli, Sandro
Carnevale, Matías Emanuel
Farias, Maria Eugenia
Henriquez, Fiona L.
author Mooney, Ronnie
author_facet Mooney, Ronnie
Rodgers, Kiri
Carnicelli, Sandro
Carnevale, Matías Emanuel
Farias, Maria Eugenia
Henriquez, Fiona L.
author_role author
author2 Rodgers, Kiri
Carnicelli, Sandro
Carnevale, Matías Emanuel
Farias, Maria Eugenia
Henriquez, Fiona L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ACANTHAMOEBA
INTRACELLULAR
MICROBIOME
SYMBIOSIS
topic ACANTHAMOEBA
INTRACELLULAR
MICROBIOME
SYMBIOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acanthamoeba spp. are widespread protists that feed on bacteria via phagocytosis. This predation pressure has led many bacteria to evolve strategies to resist and survive inside these protists. The impact of this is not well understood, but it may limit detection and allow survival in extreme environments. Three sites in the Puna salt plains, Catamarca province, Argentina, were sampled for Acanthamoeba spp., verified using PCR and Sanger sequencing. The intracellular microbiome was analysed with 16S rRNA gene sequencing and compared to the overall site microbiome. Acanthamoeba were found at all locations, and their intracellular microbiome was similar across samples but differed from the overall site microbiome. Pseudomonas spp., a clinically relevant genus, was most abundant in all isolates. This study suggests Acanthamoeba can protect bacteria, aiding their detection avoidance and survival in harsh conditions.
Fil: Mooney, Ronnie. University of Strathclyde; Reino Unido
Fil: Rodgers, Kiri. University Of The West Of Scotland; Reino Unido
Fil: Carnicelli, Sandro. University Of The West Of Scotland; Reino Unido
Fil: Carnevale, Matías Emanuel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Centro Regional de Energia y Ambiente Para El Desarrollo Sustentable. - Universidad Nacional de Catamarca. Centro Regional de Energia y Ambiente Para El Desarrollo Sustentable.; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Henriquez, Fiona L.. University of Strathclyde; Reino Unido
description Acanthamoeba spp. are widespread protists that feed on bacteria via phagocytosis. This predation pressure has led many bacteria to evolve strategies to resist and survive inside these protists. The impact of this is not well understood, but it may limit detection and allow survival in extreme environments. Three sites in the Puna salt plains, Catamarca province, Argentina, were sampled for Acanthamoeba spp., verified using PCR and Sanger sequencing. The intracellular microbiome was analysed with 16S rRNA gene sequencing and compared to the overall site microbiome. Acanthamoeba were found at all locations, and their intracellular microbiome was similar across samples but differed from the overall site microbiome. Pseudomonas spp., a clinically relevant genus, was most abundant in all isolates. This study suggests Acanthamoeba can protect bacteria, aiding their detection avoidance and survival in harsh conditions.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261480
Mooney, Ronnie; Rodgers, Kiri; Carnicelli, Sandro; Carnevale, Matías Emanuel; Farias, Maria Eugenia; et al.; Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina; John Wiley & Sons; Environmental Microbiology Reports; 17; 1; 11-2024; 1-10
1758-2229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261480
identifier_str_mv Mooney, Ronnie; Rodgers, Kiri; Carnicelli, Sandro; Carnevale, Matías Emanuel; Farias, Maria Eugenia; et al.; Isolation of Acanthamoeba species and variability in the symbiont bacterial profiles from the Puna salt plains, Catamarca province, Argentina; John Wiley & Sons; Environmental Microbiology Reports; 17; 1; 11-2024; 1-10
1758-2229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.70059
info:eu-repo/semantics/altIdentifier/doi/10.1111/1758-2229.70059
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613519450832896
score 13.070432

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