Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells

Autores
Benavente, Micaela Andrea; Bianchi, Carolina Paula; Imperiale, Fernanda Andrea; Aba, Marcelo Alfredo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Neoplasms of the mammary gland represent the most frequent tumor type in the female dog, and according to the histologic criteria, approximately 50% are malignant. In the most aggressive cases of mammary cancer, surgery is not enough to warrant a favorable outcome, and adjuvant therapies are needed to improve the patient?s overall survival. Several in vitro studies performed on murine and human cancer cells have suggested that some peptide hormones can modulate tumor growth. However, little is known about its effects on canine cancer. The aim of the present study was to evaluate the effects of Oxytocin (OT) and Desmopressin (DDAVP) on proliferation of the canine mammary cancer cell line CMT-U27. The cells were grown in 96-well plates, at two densities (4x103 and 8x103 cells/well) in 200 µl of RPMI-1640 supplemented with 10% Fetal Bovine Serum. After overnight culture, the medium was removed and replaced with medium containing OT or DDAVP at five concentrations: 10, 50, 100, 500 and 1000 nM, or medium without drugs (controls). After 72 h of incubation, cell viability was determined using the MTT colorimetric dye reduction method. With 4x103 cells/well, OT at 1000 nM resulted in a 25% of inhibition of cell viability (p<0.01). Surprisingly, OT 50 nM resulted in a higher inhibitory effect (18%) than 500 nM (9%). At 8x103 cells/well, OT showed a significant antiproliferative effect (almost 25%) with the highest concentration (p<0.05). Desmopressin at 1000 nM and 4x103 cells/well reduced cell growth by 22% (p<0.05). At 50, 100, 500 nM the inhibitory effect was lower. With 8x103cells/well, DDAVP at 100, 500 and 1000 nM exhibited a mild antiproliferative effect (p>0.05). In conclusion, OT and DDAVP can inhibit proliferation of CMT-U27 cells, by almost 20% at the highest concentrations. Further studies are required to evaluate its potential as antitumor agents, alone or combined with conventional cytotoxic drugs, for the treatment of dogs with advanced mammary cancer.
Fil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Imperiale, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicina
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
DESMOPRESSIN
OXYTOCIN
MAMMARY CANCER CELLS
CANINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184786
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184786
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cellsBenavente, Micaela AndreaBianchi, Carolina PaulaImperiale, Fernanda AndreaAba, Marcelo AlfredoDESMOPRESSINOXYTOCINMAMMARY CANCER CELLSCANINEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Neoplasms of the mammary gland represent the most frequent tumor type in the female dog, and according to the histologic criteria, approximately 50% are malignant. In the most aggressive cases of mammary cancer, surgery is not enough to warrant a favorable outcome, and adjuvant therapies are needed to improve the patient?s overall survival. Several in vitro studies performed on murine and human cancer cells have suggested that some peptide hormones can modulate tumor growth. However, little is known about its effects on canine cancer. The aim of the present study was to evaluate the effects of Oxytocin (OT) and Desmopressin (DDAVP) on proliferation of the canine mammary cancer cell line CMT-U27. The cells were grown in 96-well plates, at two densities (4x103 and 8x103 cells/well) in 200 µl of RPMI-1640 supplemented with 10% Fetal Bovine Serum. After overnight culture, the medium was removed and replaced with medium containing OT or DDAVP at five concentrations: 10, 50, 100, 500 and 1000 nM, or medium without drugs (controls). After 72 h of incubation, cell viability was determined using the MTT colorimetric dye reduction method. With 4x103 cells/well, OT at 1000 nM resulted in a 25% of inhibition of cell viability (p<0.01). Surprisingly, OT 50 nM resulted in a higher inhibitory effect (18%) than 500 nM (9%). At 8x103 cells/well, OT showed a significant antiproliferative effect (almost 25%) with the highest concentration (p<0.05). Desmopressin at 1000 nM and 4x103 cells/well reduced cell growth by 22% (p<0.05). At 50, 100, 500 nM the inhibitory effect was lower. With 8x103cells/well, DDAVP at 100, 500 and 1000 nM exhibited a mild antiproliferative effect (p>0.05). In conclusion, OT and DDAVP can inhibit proliferation of CMT-U27 cells, by almost 20% at the highest concentrations. Further studies are required to evaluate its potential as antitumor agents, alone or combined with conventional cytotoxic drugs, for the treatment of dogs with advanced mammary cancer.Fil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Imperiale, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaLXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinaAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184786Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2016; 141-1421669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biologia.org.ar/eventos/congreso/info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reunion-anualNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:45Zoai:ri.conicet.gov.ar:11336/184786instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:45.98CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
title Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
spellingShingle Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
Benavente, Micaela Andrea
DESMOPRESSIN
OXYTOCIN
MAMMARY CANCER CELLS
CANINE
title_short Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
title_full Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
title_fullStr Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
title_full_unstemmed Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
title_sort Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells
dc.creator.none.fl_str_mv Benavente, Micaela Andrea
Bianchi, Carolina Paula
Imperiale, Fernanda Andrea
Aba, Marcelo Alfredo
author Benavente, Micaela Andrea
author_facet Benavente, Micaela Andrea
Bianchi, Carolina Paula
Imperiale, Fernanda Andrea
Aba, Marcelo Alfredo
author_role author
author2 Bianchi, Carolina Paula
Imperiale, Fernanda Andrea
Aba, Marcelo Alfredo
author2_role author
author
author
dc.subject.none.fl_str_mv DESMOPRESSIN
OXYTOCIN
MAMMARY CANCER CELLS
CANINE
topic DESMOPRESSIN
OXYTOCIN
MAMMARY CANCER CELLS
CANINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Neoplasms of the mammary gland represent the most frequent tumor type in the female dog, and according to the histologic criteria, approximately 50% are malignant. In the most aggressive cases of mammary cancer, surgery is not enough to warrant a favorable outcome, and adjuvant therapies are needed to improve the patient?s overall survival. Several in vitro studies performed on murine and human cancer cells have suggested that some peptide hormones can modulate tumor growth. However, little is known about its effects on canine cancer. The aim of the present study was to evaluate the effects of Oxytocin (OT) and Desmopressin (DDAVP) on proliferation of the canine mammary cancer cell line CMT-U27. The cells were grown in 96-well plates, at two densities (4x103 and 8x103 cells/well) in 200 µl of RPMI-1640 supplemented with 10% Fetal Bovine Serum. After overnight culture, the medium was removed and replaced with medium containing OT or DDAVP at five concentrations: 10, 50, 100, 500 and 1000 nM, or medium without drugs (controls). After 72 h of incubation, cell viability was determined using the MTT colorimetric dye reduction method. With 4x103 cells/well, OT at 1000 nM resulted in a 25% of inhibition of cell viability (p<0.01). Surprisingly, OT 50 nM resulted in a higher inhibitory effect (18%) than 500 nM (9%). At 8x103 cells/well, OT showed a significant antiproliferative effect (almost 25%) with the highest concentration (p<0.05). Desmopressin at 1000 nM and 4x103 cells/well reduced cell growth by 22% (p<0.05). At 50, 100, 500 nM the inhibitory effect was lower. With 8x103cells/well, DDAVP at 100, 500 and 1000 nM exhibited a mild antiproliferative effect (p>0.05). In conclusion, OT and DDAVP can inhibit proliferation of CMT-U27 cells, by almost 20% at the highest concentrations. Further studies are required to evaluate its potential as antitumor agents, alone or combined with conventional cytotoxic drugs, for the treatment of dogs with advanced mammary cancer.
Fil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Imperiale, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicina
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Neoplasms of the mammary gland represent the most frequent tumor type in the female dog, and according to the histologic criteria, approximately 50% are malignant. In the most aggressive cases of mammary cancer, surgery is not enough to warrant a favorable outcome, and adjuvant therapies are needed to improve the patient?s overall survival. Several in vitro studies performed on murine and human cancer cells have suggested that some peptide hormones can modulate tumor growth. However, little is known about its effects on canine cancer. The aim of the present study was to evaluate the effects of Oxytocin (OT) and Desmopressin (DDAVP) on proliferation of the canine mammary cancer cell line CMT-U27. The cells were grown in 96-well plates, at two densities (4x103 and 8x103 cells/well) in 200 µl of RPMI-1640 supplemented with 10% Fetal Bovine Serum. After overnight culture, the medium was removed and replaced with medium containing OT or DDAVP at five concentrations: 10, 50, 100, 500 and 1000 nM, or medium without drugs (controls). After 72 h of incubation, cell viability was determined using the MTT colorimetric dye reduction method. With 4x103 cells/well, OT at 1000 nM resulted in a 25% of inhibition of cell viability (p<0.01). Surprisingly, OT 50 nM resulted in a higher inhibitory effect (18%) than 500 nM (9%). At 8x103 cells/well, OT showed a significant antiproliferative effect (almost 25%) with the highest concentration (p<0.05). Desmopressin at 1000 nM and 4x103 cells/well reduced cell growth by 22% (p<0.05). At 50, 100, 500 nM the inhibitory effect was lower. With 8x103cells/well, DDAVP at 100, 500 and 1000 nM exhibited a mild antiproliferative effect (p>0.05). In conclusion, OT and DDAVP can inhibit proliferation of CMT-U27 cells, by almost 20% at the highest concentrations. Further studies are required to evaluate its potential as antitumor agents, alone or combined with conventional cytotoxic drugs, for the treatment of dogs with advanced mammary cancer.
publishDate 2016
dc.date.none.fl_str_mv 2016
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Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2016; 141-142
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184786
identifier_str_mv Antiproliferative effects of oxytocin and desmopressin on canine mammary cancer cells; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXIV Reunión Anual de la Sociedad Argentina de Inmunología; XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; VII Reunión Anual de la Sociedad Argentina de Nanomedicina y V Congreso Nacional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2016; 141-142
1669-9106
CONICET Digital
CONICET
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publisher.none.fl_str_mv Fundación Revista Medicina
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