Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes

Autores
Selva, Juan Pablo; Garbus, Ingrid; Pasten, Maria Cielo; Carballo, José; Zappacosta, Diego Carlos; Pellino, Marco; Sharbel, Timoty; Echenique, Carmen Viviana
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Apomixis is an asexual reproductive process that results in seeds containing maternal clones since meiotic reduction and egg cell fertilization does not occur. Apomixis is common in polyploid grasses, and poorly represented in crop species of economic interest. A key goal in understanding the molecular basis of apomixis is the possible transference of this trait to species of agronomic relevance. Weeping lovegrass (Eragrostis curvula [Schrad.] Nees) is a perennial grass native to Southern Africa that reproduces by diplosporous apomixis. A custom 60-mer spots Agilent array was designed with 970 k probes based on a reference transcriptome constructed from inflorescence of sexual and apomictic genotypes. Eight independent hybridizations were performed with one-color Cy3 labelled samples (four apomictic and four sexual E. curvula genotypes). Data preprocessing and analysis was performed using the GeneSpring software v. 14.5. Platform quality was verified by Agilent spike-in controls. Normalization procedures consisted on percentile shift 75, background correction, median as baseline and reproductive mode as parameter. Individual probes from the array were considered to be differentially hybridized under the log2 transformed Fold Change data > 1 and p-values > 0.01 using unpaired t-test and multiple Benjamini-Hochberg correction. From these analyses 138 differential 60-mer sequences were obtained and four candidate genes strongly expressed in apomictic plants were identified. These genes could be have an important role in apomeiosis induction in weeping lovegrass and its relevance is currently being analysed through several complementary approaches, including in situ hybridization and obtention of Arabidopsis transformant.
Fil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Pellino, Marco. Global Institute for Food Security; Canadá
Fil: Sharbel, Timoty. Global Institute for Food Security; Canadá
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Plant and Animal Genome XXVI Conference (PAG)
San Diego
Estados Unidos
Plant and Animal Genome
Materia
MICROARRAYS
APOMIXIS
ERAGROSTIS CURVULA
GENE EXPRESSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225697
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/225697
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypesSelva, Juan PabloGarbus, IngridPasten, Maria CieloCarballo, JoséZappacosta, Diego CarlosPellino, MarcoSharbel, TimotyEchenique, Carmen VivianaMICROARRAYSAPOMIXISERAGROSTIS CURVULAGENE EXPRESSIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Apomixis is an asexual reproductive process that results in seeds containing maternal clones since meiotic reduction and egg cell fertilization does not occur. Apomixis is common in polyploid grasses, and poorly represented in crop species of economic interest. A key goal in understanding the molecular basis of apomixis is the possible transference of this trait to species of agronomic relevance. Weeping lovegrass (Eragrostis curvula [Schrad.] Nees) is a perennial grass native to Southern Africa that reproduces by diplosporous apomixis. A custom 60-mer spots Agilent array was designed with 970 k probes based on a reference transcriptome constructed from inflorescence of sexual and apomictic genotypes. Eight independent hybridizations were performed with one-color Cy3 labelled samples (four apomictic and four sexual E. curvula genotypes). Data preprocessing and analysis was performed using the GeneSpring software v. 14.5. Platform quality was verified by Agilent spike-in controls. Normalization procedures consisted on percentile shift 75, background correction, median as baseline and reproductive mode as parameter. Individual probes from the array were considered to be differentially hybridized under the log2 transformed Fold Change data > 1 and p-values > 0.01 using unpaired t-test and multiple Benjamini-Hochberg correction. From these analyses 138 differential 60-mer sequences were obtained and four candidate genes strongly expressed in apomictic plants were identified. These genes could be have an important role in apomeiosis induction in weeping lovegrass and its relevance is currently being analysed through several complementary approaches, including in situ hybridization and obtention of Arabidopsis transformant.Fil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; ArgentinaFil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Pellino, Marco. Global Institute for Food Security; CanadáFil: Sharbel, Timoty. Global Institute for Food Security; CanadáFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaPlant and Animal Genome XXVI Conference (PAG)San DiegoEstados UnidosPlant and Animal GenomePlant and Animal Genome2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225697Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes; Plant and Animal Genome XXVI Conference (PAG); San Diego; Estados Unidos; 2018; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.intlpag.orginfo:eu-repo/semantics/altIdentifier/url/https://plan.core-apps.com/pag_2018/abstract/ea4431cd14b6570e9d2f2a543c1ddfc6Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:56Zoai:ri.conicet.gov.ar:11336/225697instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:57.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
title Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
spellingShingle Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
Selva, Juan Pablo
MICROARRAYS
APOMIXIS
ERAGROSTIS CURVULA
GENE EXPRESSION
title_short Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
title_full Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
title_fullStr Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
title_full_unstemmed Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
title_sort Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes
dc.creator.none.fl_str_mv Selva, Juan Pablo
Garbus, Ingrid
Pasten, Maria Cielo
Carballo, José
Zappacosta, Diego Carlos
Pellino, Marco
Sharbel, Timoty
Echenique, Carmen Viviana
author Selva, Juan Pablo
author_facet Selva, Juan Pablo
Garbus, Ingrid
Pasten, Maria Cielo
Carballo, José
Zappacosta, Diego Carlos
Pellino, Marco
Sharbel, Timoty
Echenique, Carmen Viviana
author_role author
author2 Garbus, Ingrid
Pasten, Maria Cielo
Carballo, José
Zappacosta, Diego Carlos
Pellino, Marco
Sharbel, Timoty
Echenique, Carmen Viviana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MICROARRAYS
APOMIXIS
ERAGROSTIS CURVULA
GENE EXPRESSION
topic MICROARRAYS
APOMIXIS
ERAGROSTIS CURVULA
GENE EXPRESSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Apomixis is an asexual reproductive process that results in seeds containing maternal clones since meiotic reduction and egg cell fertilization does not occur. Apomixis is common in polyploid grasses, and poorly represented in crop species of economic interest. A key goal in understanding the molecular basis of apomixis is the possible transference of this trait to species of agronomic relevance. Weeping lovegrass (Eragrostis curvula [Schrad.] Nees) is a perennial grass native to Southern Africa that reproduces by diplosporous apomixis. A custom 60-mer spots Agilent array was designed with 970 k probes based on a reference transcriptome constructed from inflorescence of sexual and apomictic genotypes. Eight independent hybridizations were performed with one-color Cy3 labelled samples (four apomictic and four sexual E. curvula genotypes). Data preprocessing and analysis was performed using the GeneSpring software v. 14.5. Platform quality was verified by Agilent spike-in controls. Normalization procedures consisted on percentile shift 75, background correction, median as baseline and reproductive mode as parameter. Individual probes from the array were considered to be differentially hybridized under the log2 transformed Fold Change data > 1 and p-values > 0.01 using unpaired t-test and multiple Benjamini-Hochberg correction. From these analyses 138 differential 60-mer sequences were obtained and four candidate genes strongly expressed in apomictic plants were identified. These genes could be have an important role in apomeiosis induction in weeping lovegrass and its relevance is currently being analysed through several complementary approaches, including in situ hybridization and obtention of Arabidopsis transformant.
Fil: Selva, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Pellino, Marco. Global Institute for Food Security; Canadá
Fil: Sharbel, Timoty. Global Institute for Food Security; Canadá
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Plant and Animal Genome XXVI Conference (PAG)
San Diego
Estados Unidos
Plant and Animal Genome
description Apomixis is an asexual reproductive process that results in seeds containing maternal clones since meiotic reduction and egg cell fertilization does not occur. Apomixis is common in polyploid grasses, and poorly represented in crop species of economic interest. A key goal in understanding the molecular basis of apomixis is the possible transference of this trait to species of agronomic relevance. Weeping lovegrass (Eragrostis curvula [Schrad.] Nees) is a perennial grass native to Southern Africa that reproduces by diplosporous apomixis. A custom 60-mer spots Agilent array was designed with 970 k probes based on a reference transcriptome constructed from inflorescence of sexual and apomictic genotypes. Eight independent hybridizations were performed with one-color Cy3 labelled samples (four apomictic and four sexual E. curvula genotypes). Data preprocessing and analysis was performed using the GeneSpring software v. 14.5. Platform quality was verified by Agilent spike-in controls. Normalization procedures consisted on percentile shift 75, background correction, median as baseline and reproductive mode as parameter. Individual probes from the array were considered to be differentially hybridized under the log2 transformed Fold Change data > 1 and p-values > 0.01 using unpaired t-test and multiple Benjamini-Hochberg correction. From these analyses 138 differential 60-mer sequences were obtained and four candidate genes strongly expressed in apomictic plants were identified. These genes could be have an important role in apomeiosis induction in weeping lovegrass and its relevance is currently being analysed through several complementary approaches, including in situ hybridization and obtention of Arabidopsis transformant.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225697
Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes; Plant and Animal Genome XXVI Conference (PAG); San Diego; Estados Unidos; 2018; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225697
identifier_str_mv Array-based comparative study of apomicitc and sexual Eragrostis curvula genotypes; Plant and Animal Genome XXVI Conference (PAG); San Diego; Estados Unidos; 2018; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/https://plan.core-apps.com/pag_2018/abstract/ea4431cd14b6570e9d2f2a543c1ddfc6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Plant and Animal Genome
publisher.none.fl_str_mv Plant and Animal Genome
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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