Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds

Autores
Figueroa, Carlos Maria; Piattoni, Claudia Vanesa; Asención Diez, Matías Damián; Comelli, Raul Alberto; Guerrero, Sergio Adrian; Beccaria, Alejandro José; Iglesias, Alberto Alvaro
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glycerol (Gro) has many appliances in the cosmetic, food, and pharmaceutical industries. However, Gro has become an inexpensive carbon source due to its generation as inevitable by-product of the biodiesel production, a process with outstanding growing within the last decade. In addition, the excess of Gro has environmental problems since it is not innocuous and its disposal turns complex. Thus, conversion of low-priced Gro streams to higher value products, such as 1,3-propanediol or dihydroxyacetone (DHA), has been proposed as relevant biorefinery strategies. Many organisms are able to metabolize Gro, and their enzymes are key targets in the bioconversion of this compound. In this work we have cloned and expressed the gene encoding for Gro dehydrogenase (GroDHase) in Escherichia coli. The enzyme converts Gro into DHA. The recombinant enzyme was purified to near homogeneity either by IMAC or heat-shock treatment. We have analyzed the activity assay conditions and determined the S0.5 values for both substrates, NAD+ (0,5 mM) and Gro (100 mM). The maximum activity was reached at pH 11.0 and 40 ºC. NH4Cl was found to be an activator of GroDHase, reducing (4-fold) the S0.5 for Gro and increasing (1.5-fold) the Vmax. Gro waste derived from biodiesel production was an effective substrate for the enzyme, which supports the biotechnological application of the recombinant GroDHase.
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Piattoni, Claudia Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Comelli, Raul Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera". Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera"; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Beccaria, Alejandro José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
Glycerol
Dihydroxyacetone
1,3-Propanediol
Biorefineries
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57019
Acceder
id CONICETDig_3b6d9424462286bf52ce47ef30297cea
oai_identifier_str oai:ri.conicet.gov.ar:11336/57019
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compoundsFigueroa, Carlos MariaPiattoni, Claudia VanesaAsención Diez, Matías DamiánComelli, Raul AlbertoGuerrero, Sergio AdrianBeccaria, Alejandro JoséIglesias, Alberto AlvaroGlycerolDihydroxyacetone1,3-PropanediolBiorefinerieshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Glycerol (Gro) has many appliances in the cosmetic, food, and pharmaceutical industries. However, Gro has become an inexpensive carbon source due to its generation as inevitable by-product of the biodiesel production, a process with outstanding growing within the last decade. In addition, the excess of Gro has environmental problems since it is not innocuous and its disposal turns complex. Thus, conversion of low-priced Gro streams to higher value products, such as 1,3-propanediol or dihydroxyacetone (DHA), has been proposed as relevant biorefinery strategies. Many organisms are able to metabolize Gro, and their enzymes are key targets in the bioconversion of this compound. In this work we have cloned and expressed the gene encoding for Gro dehydrogenase (GroDHase) in Escherichia coli. The enzyme converts Gro into DHA. The recombinant enzyme was purified to near homogeneity either by IMAC or heat-shock treatment. We have analyzed the activity assay conditions and determined the S0.5 values for both substrates, NAD+ (0,5 mM) and Gro (100 mM). The maximum activity was reached at pH 11.0 and 40 ºC. NH4Cl was found to be an activator of GroDHase, reducing (4-fold) the S0.5 for Gro and increasing (1.5-fold) the Vmax. Gro waste derived from biodiesel production was an effective substrate for the enzyme, which supports the biotechnological application of the recombinant GroDHase.Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Piattoni, Claudia Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Comelli, Raul Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera". Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera"; ArgentinaFil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Beccaria, Alejandro José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaInstituto de Histología y Embriología2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57019Figueroa, Carlos Maria; Piattoni, Claudia Vanesa; Asención Diez, Matías Damián; Comelli, Raul Alberto; Guerrero, Sergio Adrian; et al.; Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds; Instituto de Histología y Embriología; Biocell; 33; 10-2009; 55-550327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cricyt.edu.ar/biocell/saib_2009.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:21:53Zoai:ri.conicet.gov.ar:11336/57019instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:21:53.335CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
title Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
spellingShingle Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
Figueroa, Carlos Maria
Glycerol
Dihydroxyacetone
1,3-Propanediol
Biorefineries
title_short Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
title_full Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
title_fullStr Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
title_full_unstemmed Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
title_sort Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds
dc.creator.none.fl_str_mv Figueroa, Carlos Maria
Piattoni, Claudia Vanesa
Asención Diez, Matías Damián
Comelli, Raul Alberto
Guerrero, Sergio Adrian
Beccaria, Alejandro José
Iglesias, Alberto Alvaro
author Figueroa, Carlos Maria
author_facet Figueroa, Carlos Maria
Piattoni, Claudia Vanesa
Asención Diez, Matías Damián
Comelli, Raul Alberto
Guerrero, Sergio Adrian
Beccaria, Alejandro José
Iglesias, Alberto Alvaro
author_role author
author2 Piattoni, Claudia Vanesa
Asención Diez, Matías Damián
Comelli, Raul Alberto
Guerrero, Sergio Adrian
Beccaria, Alejandro José
Iglesias, Alberto Alvaro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Glycerol
Dihydroxyacetone
1,3-Propanediol
Biorefineries
topic Glycerol
Dihydroxyacetone
1,3-Propanediol
Biorefineries
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Glycerol (Gro) has many appliances in the cosmetic, food, and pharmaceutical industries. However, Gro has become an inexpensive carbon source due to its generation as inevitable by-product of the biodiesel production, a process with outstanding growing within the last decade. In addition, the excess of Gro has environmental problems since it is not innocuous and its disposal turns complex. Thus, conversion of low-priced Gro streams to higher value products, such as 1,3-propanediol or dihydroxyacetone (DHA), has been proposed as relevant biorefinery strategies. Many organisms are able to metabolize Gro, and their enzymes are key targets in the bioconversion of this compound. In this work we have cloned and expressed the gene encoding for Gro dehydrogenase (GroDHase) in Escherichia coli. The enzyme converts Gro into DHA. The recombinant enzyme was purified to near homogeneity either by IMAC or heat-shock treatment. We have analyzed the activity assay conditions and determined the S0.5 values for both substrates, NAD+ (0,5 mM) and Gro (100 mM). The maximum activity was reached at pH 11.0 and 40 ºC. NH4Cl was found to be an activator of GroDHase, reducing (4-fold) the S0.5 for Gro and increasing (1.5-fold) the Vmax. Gro waste derived from biodiesel production was an effective substrate for the enzyme, which supports the biotechnological application of the recombinant GroDHase.
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Piattoni, Claudia Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Comelli, Raul Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera". Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica "Ing. José Miguel Parera"; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Beccaria, Alejandro José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description Glycerol (Gro) has many appliances in the cosmetic, food, and pharmaceutical industries. However, Gro has become an inexpensive carbon source due to its generation as inevitable by-product of the biodiesel production, a process with outstanding growing within the last decade. In addition, the excess of Gro has environmental problems since it is not innocuous and its disposal turns complex. Thus, conversion of low-priced Gro streams to higher value products, such as 1,3-propanediol or dihydroxyacetone (DHA), has been proposed as relevant biorefinery strategies. Many organisms are able to metabolize Gro, and their enzymes are key targets in the bioconversion of this compound. In this work we have cloned and expressed the gene encoding for Gro dehydrogenase (GroDHase) in Escherichia coli. The enzyme converts Gro into DHA. The recombinant enzyme was purified to near homogeneity either by IMAC or heat-shock treatment. We have analyzed the activity assay conditions and determined the S0.5 values for both substrates, NAD+ (0,5 mM) and Gro (100 mM). The maximum activity was reached at pH 11.0 and 40 ºC. NH4Cl was found to be an activator of GroDHase, reducing (4-fold) the S0.5 for Gro and increasing (1.5-fold) the Vmax. Gro waste derived from biodiesel production was an effective substrate for the enzyme, which supports the biotechnological application of the recombinant GroDHase.
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57019
Figueroa, Carlos Maria; Piattoni, Claudia Vanesa; Asención Diez, Matías Damián; Comelli, Raul Alberto; Guerrero, Sergio Adrian; et al.; Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds; Instituto de Histología y Embriología; Biocell; 33; 10-2009; 55-55
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57019
identifier_str_mv Figueroa, Carlos Maria; Piattoni, Claudia Vanesa; Asención Diez, Matías Damián; Comelli, Raul Alberto; Guerrero, Sergio Adrian; et al.; Harnessing glycerol from biodiesel fuel production to obtain valuable industrial compounds; Instituto de Histología y Embriología; Biocell; 33; 10-2009; 55-55
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cricyt.edu.ar/biocell/saib_2009.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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