Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans

Autores
Romanowski, Andrés; Migliori, María Laura; Valverde, Claudio Fabián; Golombek, Diego Andrés
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abiotic and biotic environmental stressors play a key role in the ecophysiology of most organisms. As the presence and activity of stress-inducing agents vary along the day, organisms that are able to predict these periodic changes are better fit to survive. Caenorhabditis elegans, a soil-dwelling nematode, is subjected to daily changes in its natural environment, and its tolerance to osmotic and oxidative stress varies along the day. Pseudomonas fluorescens strain CHA0 is a soil bacterium that produces a set of secondary metabolites that antagonize phytopathogenic fungi and therefore promote healthy growth of several plant species. Here we show that strain CHA0 is able to affect C. elegans either under growth limiting conditions (i.e., slow-killing) or by rapid paralysis in nutrient replete conditions (fast-killing). Both types of toxicity require the post-transcriptional Gac/Rsm regulatory cascade, and the fast paralytic killing depends strongly on hydrogen cyanide production. The response observed in C. elegans nematodes to fast paralytic killing varies along the day and its sensitivity is higher during the night, at Zeitgeber Time (ZT) 12 (lights off). This behavior correlates well with HCN tolerance, which is higher during the day, at ZT0 (lights on). The innate immune response to P. fluorescens CHA0 might depend on the stress response pathway of C. elegans. The fact that the tolerance varies daily gives further proof of an underlying clock that governs cyclic behavior in C. elegans. © 2010 Elsevier Ltd.
Fil: Romanowski, Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Migliori, María Laura. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Golombek, Diego Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Materia
Caenorhabditis Elegans
Circadian Rhythms
Cyanide
Daily Variation
Fast Paralytic Killing
Pseudomonas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53647
Acceder
id CONICETDig_3b427f070f906a973c02fb5a2ae2daea
oai_identifier_str oai:ri.conicet.gov.ar:11336/53647
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegansRomanowski, AndrésMigliori, María LauraValverde, Claudio FabiánGolombek, Diego AndrésCaenorhabditis ElegansCircadian RhythmsCyanideDaily VariationFast Paralytic KillingPseudomonashttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Abiotic and biotic environmental stressors play a key role in the ecophysiology of most organisms. As the presence and activity of stress-inducing agents vary along the day, organisms that are able to predict these periodic changes are better fit to survive. Caenorhabditis elegans, a soil-dwelling nematode, is subjected to daily changes in its natural environment, and its tolerance to osmotic and oxidative stress varies along the day. Pseudomonas fluorescens strain CHA0 is a soil bacterium that produces a set of secondary metabolites that antagonize phytopathogenic fungi and therefore promote healthy growth of several plant species. Here we show that strain CHA0 is able to affect C. elegans either under growth limiting conditions (i.e., slow-killing) or by rapid paralysis in nutrient replete conditions (fast-killing). Both types of toxicity require the post-transcriptional Gac/Rsm regulatory cascade, and the fast paralytic killing depends strongly on hydrogen cyanide production. The response observed in C. elegans nematodes to fast paralytic killing varies along the day and its sensitivity is higher during the night, at Zeitgeber Time (ZT) 12 (lights off). This behavior correlates well with HCN tolerance, which is higher during the day, at ZT0 (lights on). The innate immune response to P. fluorescens CHA0 might depend on the stress response pathway of C. elegans. The fact that the tolerance varies daily gives further proof of an underlying clock that governs cyclic behavior in C. elegans. © 2010 Elsevier Ltd.Fil: Romanowski, Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Migliori, María Laura. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Golombek, Diego Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53647Romanowski, Andrés; Migliori, María Laura; Valverde, Claudio Fabián; Golombek, Diego Andrés; Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 50; 1; 1-2011; 23-300882-4010CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2010.09.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0882401010001774info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:23:38Zoai:ri.conicet.gov.ar:11336/53647instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:23:38.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
title Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
spellingShingle Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
Romanowski, Andrés
Caenorhabditis Elegans
Circadian Rhythms
Cyanide
Daily Variation
Fast Paralytic Killing
Pseudomonas
title_short Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
title_full Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
title_fullStr Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
title_full_unstemmed Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
title_sort Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans
dc.creator.none.fl_str_mv Romanowski, Andrés
Migliori, María Laura
Valverde, Claudio Fabián
Golombek, Diego Andrés
author Romanowski, Andrés
author_facet Romanowski, Andrés
Migliori, María Laura
Valverde, Claudio Fabián
Golombek, Diego Andrés
author_role author
author2 Migliori, María Laura
Valverde, Claudio Fabián
Golombek, Diego Andrés
author2_role author
author
author
dc.subject.none.fl_str_mv Caenorhabditis Elegans
Circadian Rhythms
Cyanide
Daily Variation
Fast Paralytic Killing
Pseudomonas
topic Caenorhabditis Elegans
Circadian Rhythms
Cyanide
Daily Variation
Fast Paralytic Killing
Pseudomonas
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Abiotic and biotic environmental stressors play a key role in the ecophysiology of most organisms. As the presence and activity of stress-inducing agents vary along the day, organisms that are able to predict these periodic changes are better fit to survive. Caenorhabditis elegans, a soil-dwelling nematode, is subjected to daily changes in its natural environment, and its tolerance to osmotic and oxidative stress varies along the day. Pseudomonas fluorescens strain CHA0 is a soil bacterium that produces a set of secondary metabolites that antagonize phytopathogenic fungi and therefore promote healthy growth of several plant species. Here we show that strain CHA0 is able to affect C. elegans either under growth limiting conditions (i.e., slow-killing) or by rapid paralysis in nutrient replete conditions (fast-killing). Both types of toxicity require the post-transcriptional Gac/Rsm regulatory cascade, and the fast paralytic killing depends strongly on hydrogen cyanide production. The response observed in C. elegans nematodes to fast paralytic killing varies along the day and its sensitivity is higher during the night, at Zeitgeber Time (ZT) 12 (lights off). This behavior correlates well with HCN tolerance, which is higher during the day, at ZT0 (lights on). The innate immune response to P. fluorescens CHA0 might depend on the stress response pathway of C. elegans. The fact that the tolerance varies daily gives further proof of an underlying clock that governs cyclic behavior in C. elegans. © 2010 Elsevier Ltd.
Fil: Romanowski, Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Migliori, María Laura. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Valverde, Claudio Fabián. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Golombek, Diego Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
description Abiotic and biotic environmental stressors play a key role in the ecophysiology of most organisms. As the presence and activity of stress-inducing agents vary along the day, organisms that are able to predict these periodic changes are better fit to survive. Caenorhabditis elegans, a soil-dwelling nematode, is subjected to daily changes in its natural environment, and its tolerance to osmotic and oxidative stress varies along the day. Pseudomonas fluorescens strain CHA0 is a soil bacterium that produces a set of secondary metabolites that antagonize phytopathogenic fungi and therefore promote healthy growth of several plant species. Here we show that strain CHA0 is able to affect C. elegans either under growth limiting conditions (i.e., slow-killing) or by rapid paralysis in nutrient replete conditions (fast-killing). Both types of toxicity require the post-transcriptional Gac/Rsm regulatory cascade, and the fast paralytic killing depends strongly on hydrogen cyanide production. The response observed in C. elegans nematodes to fast paralytic killing varies along the day and its sensitivity is higher during the night, at Zeitgeber Time (ZT) 12 (lights off). This behavior correlates well with HCN tolerance, which is higher during the day, at ZT0 (lights on). The innate immune response to P. fluorescens CHA0 might depend on the stress response pathway of C. elegans. The fact that the tolerance varies daily gives further proof of an underlying clock that governs cyclic behavior in C. elegans. © 2010 Elsevier Ltd.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53647
Romanowski, Andrés; Migliori, María Laura; Valverde, Claudio Fabián; Golombek, Diego Andrés; Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 50; 1; 1-2011; 23-30
0882-4010
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53647
identifier_str_mv Romanowski, Andrés; Migliori, María Laura; Valverde, Claudio Fabián; Golombek, Diego Andrés; Circadian variation in Pseudomonas fluorescens (CHA0)-mediated paralysis of Caenorhabditis elegans; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 50; 1; 1-2011; 23-30
0882-4010
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2010.09.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0882401010001774
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981306161430528
score 12.48226

Publicaciones similares