An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)

Autores: Mascarell Creus, Albert; Cañizares, Joaquin; Vilarrasa Blasi, Josep; Mora Garcia, Santiago; Blanca, José; Gonzalez Ibeas, Daniel; Saladié, Montserrat; Roig, Cristina; Deleu, Wim; Picó Silvent, Belén; López Bigas, Nuria; Aranda, Miguel A.; Garcia Mas, Jordi; Nuez, Fernando; Puigdomènech, Pere; Caño Delgado, Ana I.
Año de publicación: 2009
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Background: Melon (Cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression. Results: We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study. Conclusion: The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.
Fil: Mascarell Creus, Albert. Centro de Investigación en Agrigenómica; España
Fil: Cañizares, Joaquin. Universidad Politécnica de Valencia; España
Fil: Vilarrasa Blasi, Josep. Centro de Investigación en Agrigenómica; España
Fil: Mora Garcia, Santiago. Centro de Investigación en Agrigenómica; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Blanca, José. Universidad Politécnica de Valencia; España
Fil: Gonzalez Ibeas, Daniel. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Saladié, Montserrat. Centro de Investigación en Agrigenómica; España
Fil: Roig, Cristina. Universidad Politécnica de Valencia; España
Fil: Deleu, Wim. Centro de Investigación en Agrigenómica; España
Fil: Picó Silvent, Belén. Universidad Politécnica de Valencia; España
Fil: López Bigas, Nuria. Universitat Pompeu Fabra; España
Fil: Aranda, Miguel A.. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España
Fil: Garcia Mas, Jordi. Centro de Investigación en Agrigenómica; España
Fil: Nuez, Fernando. Universidad Politécnica de Valencia; España
Fil: Puigdomènech, Pere. Centro de Investigación en Agrigenómica; España
Fil: Caño Delgado, Ana I.. Centro de Investigación en Agrigenómica; España
Materia: microarray
melon
pathogen resistance
fruit quality
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/101671

Acceder

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network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)Mascarell Creus, AlbertCañizares, JoaquinVilarrasa Blasi, JosepMora Garcia, SantiagoBlanca, JoséGonzalez Ibeas, DanielSaladié, MontserratRoig, CristinaDeleu, WimPicó Silvent, BelénLópez Bigas, NuriaAranda, Miguel A.Garcia Mas, JordiNuez, FernandoPuigdomènech, PereCaño Delgado, Ana I.microarraymelonpathogen resistancefruit qualityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Melon (Cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression. Results: We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study. Conclusion: The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.Fil: Mascarell Creus, Albert. Centro de Investigación en Agrigenómica; EspañaFil: Cañizares, Joaquin. Universidad Politécnica de Valencia; EspañaFil: Vilarrasa Blasi, Josep. Centro de Investigación en Agrigenómica; EspañaFil: Mora Garcia, Santiago. Centro de Investigación en Agrigenómica; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Blanca, José. Universidad Politécnica de Valencia; EspañaFil: Gonzalez Ibeas, Daniel. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Saladié, Montserrat. Centro de Investigación en Agrigenómica; EspañaFil: Roig, Cristina. Universidad Politécnica de Valencia; EspañaFil: Deleu, Wim. Centro de Investigación en Agrigenómica; EspañaFil: Picó Silvent, Belén. Universidad Politécnica de Valencia; EspañaFil: López Bigas, Nuria. Universitat Pompeu Fabra; EspañaFil: Aranda, Miguel A.. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; EspañaFil: Garcia Mas, Jordi. Centro de Investigación en Agrigenómica; EspañaFil: Nuez, Fernando. Universidad Politécnica de Valencia; EspañaFil: Puigdomènech, Pere. Centro de Investigación en Agrigenómica; EspañaFil: Caño Delgado, Ana I.. Centro de Investigación en Agrigenómica; EspañaBioMed Central2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101671Mascarell Creus, Albert; Cañizares, Joaquin; Vilarrasa Blasi, Josep; Mora Garcia, Santiago; Blanca, José; et al.; An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.); BioMed Central; BMC Genomics; 10; 467; 10-2009; 1-151471-21641471-2164CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-10-467info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2164-10-467info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:09:24Zoai:ri.conicet.gov.ar:11336/101671instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:09:24.327CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
title	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
spellingShingle	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.) Mascarell Creus, Albert microarray melon pathogen resistance fruit quality
title_short	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
title_full	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
title_fullStr	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
title_full_unstemmed	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
title_sort	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)
dc.creator.none.fl_str_mv	Mascarell Creus, Albert Cañizares, Joaquin Vilarrasa Blasi, Josep Mora Garcia, Santiago Blanca, José Gonzalez Ibeas, Daniel Saladié, Montserrat Roig, Cristina Deleu, Wim Picó Silvent, Belén López Bigas, Nuria Aranda, Miguel A. Garcia Mas, Jordi Nuez, Fernando Puigdomènech, Pere Caño Delgado, Ana I.
author	Mascarell Creus, Albert
author_facet	Mascarell Creus, Albert Cañizares, Joaquin Vilarrasa Blasi, Josep Mora Garcia, Santiago Blanca, José Gonzalez Ibeas, Daniel Saladié, Montserrat Roig, Cristina Deleu, Wim Picó Silvent, Belén López Bigas, Nuria Aranda, Miguel A. Garcia Mas, Jordi Nuez, Fernando Puigdomènech, Pere Caño Delgado, Ana I.
author_role	author
author2	Cañizares, Joaquin Vilarrasa Blasi, Josep Mora Garcia, Santiago Blanca, José Gonzalez Ibeas, Daniel Saladié, Montserrat Roig, Cristina Deleu, Wim Picó Silvent, Belén López Bigas, Nuria Aranda, Miguel A. Garcia Mas, Jordi Nuez, Fernando Puigdomènech, Pere Caño Delgado, Ana I.
author2_role	author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	microarray melon pathogen resistance fruit quality
topic	microarray melon pathogen resistance fruit quality
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Background: Melon (Cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression. Results: We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study. Conclusion: The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon. Fil: Mascarell Creus, Albert. Centro de Investigación en Agrigenómica; España Fil: Cañizares, Joaquin. Universidad Politécnica de Valencia; España Fil: Vilarrasa Blasi, Josep. Centro de Investigación en Agrigenómica; España Fil: Mora Garcia, Santiago. Centro de Investigación en Agrigenómica; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Blanca, José. Universidad Politécnica de Valencia; España Fil: Gonzalez Ibeas, Daniel. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España Fil: Saladié, Montserrat. Centro de Investigación en Agrigenómica; España Fil: Roig, Cristina. Universidad Politécnica de Valencia; España Fil: Deleu, Wim. Centro de Investigación en Agrigenómica; España Fil: Picó Silvent, Belén. Universidad Politécnica de Valencia; España Fil: López Bigas, Nuria. Universitat Pompeu Fabra; España Fil: Aranda, Miguel A.. Consejo Superior de Investigaciones Científicas. Centro de Edafología y Biología Aplicada del Segura; España Fil: Garcia Mas, Jordi. Centro de Investigación en Agrigenómica; España Fil: Nuez, Fernando. Universidad Politécnica de Valencia; España Fil: Puigdomènech, Pere. Centro de Investigación en Agrigenómica; España Fil: Caño Delgado, Ana I.. Centro de Investigación en Agrigenómica; España
description	Background: Melon (Cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression. Results: We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study. Conclusion: The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.
publishDate	2009
dc.date.none.fl_str_mv	2009-10
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/101671 Mascarell Creus, Albert; Cañizares, Joaquin; Vilarrasa Blasi, Josep; Mora Garcia, Santiago; Blanca, José; et al.; An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.); BioMed Central; BMC Genomics; 10; 467; 10-2009; 1-15 1471-2164 1471-2164 CONICET Digital CONICET
url	http://hdl.handle.net/11336/101671
identifier_str_mv	Mascarell Creus, Albert; Cañizares, Joaquin; Vilarrasa Blasi, Josep; Mora Garcia, Santiago; Blanca, José; et al.; An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.); BioMed Central; BMC Genomics; 10; 467; 10-2009; 1-15 1471-2164 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-10-467 info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2164-10-467
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	BioMed Central
publisher.none.fl_str_mv	BioMed Central
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)

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