Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection

Autores
Arana, Mercedes; Tettamanti, Cecilia Soledad; Bercoff, Paula Gabriela; Rodriguez, Marcela Cecilia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work reports the novel application of carbon-coated magnetite nanoparticles (mNPs@C) as catalytic nanomaterial included in a composite electrode material (mNPs@C/CPE) taking advantages of their intrinsic peroxidase-like activity. The nanostructured electrochemical transducer reveals an enhancement of the charge transfer for redox processes involving hydrogen peroxide. Likewise, mNPs@C/CPE demonstrated to be highly selective even at elevated concentrations of ascorbic acid and uric acid, the usual interferents of blood glucose analysis. Upon these remarkable results, the composite matrix was further modified by the addition of glucose oxidase as biocatalyst, in order to obtain a biosensing strategy (GOx/mNPs@C/CPE) with enhanced properties for the electrochemical detection of glucose. GOx/mNPs@C/CPE exhibit a linear range up to 7.5×10−3 mol L−1 glucose, comprising the entirely physiological range and incipient pathological values. The average sensitivity obtained at −0.100 V was (1.62±0.05)×105 nA L mol−1 (R2=0.9992), the detection limit was 2.0×10−6 M while the quantification limit was 6.1×10−6 mol L−1. The nanostructured biosensor demonstrated to have an excellent performance for glucose detection in human blood serum even for pathological values.
Fil: Arana, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Tettamanti, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bercoff, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Rodriguez, Marcela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Carbon-Coated Magnetite Nanoparticles
Electrochemical Glucose Biosensor
Glucose Oxidase
Modified-Carbon Paste Electrode
Peroxidase-Like Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31505
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose DetectionArana, MercedesTettamanti, Cecilia SoledadBercoff, Paula GabrielaRodriguez, Marcela CeciliaCarbon-Coated Magnetite NanoparticlesElectrochemical Glucose BiosensorGlucose OxidaseModified-Carbon Paste ElectrodePeroxidase-Like Activityhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work reports the novel application of carbon-coated magnetite nanoparticles (mNPs@C) as catalytic nanomaterial included in a composite electrode material (mNPs@C/CPE) taking advantages of their intrinsic peroxidase-like activity. The nanostructured electrochemical transducer reveals an enhancement of the charge transfer for redox processes involving hydrogen peroxide. Likewise, mNPs@C/CPE demonstrated to be highly selective even at elevated concentrations of ascorbic acid and uric acid, the usual interferents of blood glucose analysis. Upon these remarkable results, the composite matrix was further modified by the addition of glucose oxidase as biocatalyst, in order to obtain a biosensing strategy (GOx/mNPs@C/CPE) with enhanced properties for the electrochemical detection of glucose. GOx/mNPs@C/CPE exhibit a linear range up to 7.5×10−3 mol L−1 glucose, comprising the entirely physiological range and incipient pathological values. The average sensitivity obtained at −0.100 V was (1.62±0.05)×105 nA L mol−1 (R2=0.9992), the detection limit was 2.0×10−6 M while the quantification limit was 6.1×10−6 mol L−1. The nanostructured biosensor demonstrated to have an excellent performance for glucose detection in human blood serum even for pathological values.Fil: Arana, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Tettamanti, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bercoff, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Rodriguez, Marcela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaWiley VCH Verlag2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31505Rodriguez, Marcela Cecilia; Bercoff, Paula Gabriela; Tettamanti, Cecilia Soledad; Arana, Mercedes; Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection; Wiley VCH Verlag; Electroanalysis; 26; 8; 7-2014; 1721-17281040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elan.201400159info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.201400159/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:29Zoai:ri.conicet.gov.ar:11336/31505instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:29.738CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
title Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
spellingShingle Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
Arana, Mercedes
Carbon-Coated Magnetite Nanoparticles
Electrochemical Glucose Biosensor
Glucose Oxidase
Modified-Carbon Paste Electrode
Peroxidase-Like Activity
title_short Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
title_full Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
title_fullStr Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
title_full_unstemmed Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
title_sort Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection
dc.creator.none.fl_str_mv Arana, Mercedes
Tettamanti, Cecilia Soledad
Bercoff, Paula Gabriela
Rodriguez, Marcela Cecilia
author Arana, Mercedes
author_facet Arana, Mercedes
Tettamanti, Cecilia Soledad
Bercoff, Paula Gabriela
Rodriguez, Marcela Cecilia
author_role author
author2 Tettamanti, Cecilia Soledad
Bercoff, Paula Gabriela
Rodriguez, Marcela Cecilia
author2_role author
author
author
dc.subject.none.fl_str_mv Carbon-Coated Magnetite Nanoparticles
Electrochemical Glucose Biosensor
Glucose Oxidase
Modified-Carbon Paste Electrode
Peroxidase-Like Activity
topic Carbon-Coated Magnetite Nanoparticles
Electrochemical Glucose Biosensor
Glucose Oxidase
Modified-Carbon Paste Electrode
Peroxidase-Like Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work reports the novel application of carbon-coated magnetite nanoparticles (mNPs@C) as catalytic nanomaterial included in a composite electrode material (mNPs@C/CPE) taking advantages of their intrinsic peroxidase-like activity. The nanostructured electrochemical transducer reveals an enhancement of the charge transfer for redox processes involving hydrogen peroxide. Likewise, mNPs@C/CPE demonstrated to be highly selective even at elevated concentrations of ascorbic acid and uric acid, the usual interferents of blood glucose analysis. Upon these remarkable results, the composite matrix was further modified by the addition of glucose oxidase as biocatalyst, in order to obtain a biosensing strategy (GOx/mNPs@C/CPE) with enhanced properties for the electrochemical detection of glucose. GOx/mNPs@C/CPE exhibit a linear range up to 7.5×10−3 mol L−1 glucose, comprising the entirely physiological range and incipient pathological values. The average sensitivity obtained at −0.100 V was (1.62±0.05)×105 nA L mol−1 (R2=0.9992), the detection limit was 2.0×10−6 M while the quantification limit was 6.1×10−6 mol L−1. The nanostructured biosensor demonstrated to have an excellent performance for glucose detection in human blood serum even for pathological values.
Fil: Arana, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Tettamanti, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Bercoff, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Rodriguez, Marcela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work reports the novel application of carbon-coated magnetite nanoparticles (mNPs@C) as catalytic nanomaterial included in a composite electrode material (mNPs@C/CPE) taking advantages of their intrinsic peroxidase-like activity. The nanostructured electrochemical transducer reveals an enhancement of the charge transfer for redox processes involving hydrogen peroxide. Likewise, mNPs@C/CPE demonstrated to be highly selective even at elevated concentrations of ascorbic acid and uric acid, the usual interferents of blood glucose analysis. Upon these remarkable results, the composite matrix was further modified by the addition of glucose oxidase as biocatalyst, in order to obtain a biosensing strategy (GOx/mNPs@C/CPE) with enhanced properties for the electrochemical detection of glucose. GOx/mNPs@C/CPE exhibit a linear range up to 7.5×10−3 mol L−1 glucose, comprising the entirely physiological range and incipient pathological values. The average sensitivity obtained at −0.100 V was (1.62±0.05)×105 nA L mol−1 (R2=0.9992), the detection limit was 2.0×10−6 M while the quantification limit was 6.1×10−6 mol L−1. The nanostructured biosensor demonstrated to have an excellent performance for glucose detection in human blood serum even for pathological values.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31505
Rodriguez, Marcela Cecilia; Bercoff, Paula Gabriela; Tettamanti, Cecilia Soledad; Arana, Mercedes; Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection; Wiley VCH Verlag; Electroanalysis; 26; 8; 7-2014; 1721-1728
1040-0397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31505
identifier_str_mv Rodriguez, Marcela Cecilia; Bercoff, Paula Gabriela; Tettamanti, Cecilia Soledad; Arana, Mercedes; Magnetite NPs@C with Highly-Efficient Peroxidase-Like Catalytic Activity as an Improved Biosensing Strategy for Selective Glucose Detection; Wiley VCH Verlag; Electroanalysis; 26; 8; 7-2014; 1721-1728
1040-0397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.201400159
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.201400159/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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