Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture

Autores
Bonin, Luka; Schofs, Laureano; Pečan, Luka Irenej; Oblak, Tine; Tavčar, Gaper; Ponikvar Svet, Maja; Silverman, Julian R.; Sanchez Bruni, Sergio Fabian; Jeran, Marko
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes.
Fil: Bonin, Luka. University Of Ljubljana; Eslovenia
Fil: Schofs, Laureano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Pečan, Luka Irenej. University Of Ljubljana; Eslovenia. Università degli Studi di Trieste; Italia
Fil: Oblak, Tine. Institut Jožef Stefan; Eslovenia
Fil: Tavčar, Gaper. Institut Jožef Stefan; Eslovenia
Fil: Ponikvar Svet, Maja. Institut Jožef Stefan; Eslovenia
Fil: Silverman, Julian R.. Fashion Institute Of Technology; Estados Unidos
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Jeran, Marko. Institut Jožef Stefan; Eslovenia
Socratic Lectures: 12th International Symposium
Ljubljana
Eslovenia
University of Ljubljana. Faculty of Health Sciences
Materia
PLANT
SUPERCRITICAL CARBON DIOXIDE
WATER-ETHANOL
EXTRACTS
POLYPHENOLS
BIOLOGICALLY ACTIVE COMPOUNDS
MEDICINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268277

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network_name_str CONICET Digital (CONICET)
spelling Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixtureBonin, LukaSchofs, LaureanoPečan, Luka IrenejOblak, TineTavčar, GaperPonikvar Svet, MajaSilverman, Julian R.Sanchez Bruni, Sergio FabianJeran, MarkoPLANTSUPERCRITICAL CARBON DIOXIDEWATER-ETHANOLEXTRACTSPOLYPHENOLSBIOLOGICALLY ACTIVE COMPOUNDSMEDICINEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes.Fil: Bonin, Luka. University Of Ljubljana; EsloveniaFil: Schofs, Laureano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Pečan, Luka Irenej. University Of Ljubljana; Eslovenia. Università degli Studi di Trieste; ItaliaFil: Oblak, Tine. Institut Jožef Stefan; EsloveniaFil: Tavčar, Gaper. Institut Jožef Stefan; EsloveniaFil: Ponikvar Svet, Maja. Institut Jožef Stefan; EsloveniaFil: Silverman, Julian R.. Fashion Institute Of Technology; Estados UnidosFil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Jeran, Marko. Institut Jožef Stefan; EsloveniaSocratic Lectures: 12th International SymposiumLjubljanaEsloveniaUniversity of Ljubljana. Faculty of Health SciencesUniversity of Ljubljana. Faculty of Health Sciences2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268277Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture; Socratic Lectures: 12th International Symposium; Ljubljana; Eslovenia; 2025; 51-58978-961-297-551-7CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ebooks.uni-lj.si/ZalozbaUL/catalog/book/742info:eu-repo/semantics/altIdentifier/doi/10.55295/PSL.12.2025.II5Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:52Zoai:ri.conicet.gov.ar:11336/268277instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:52.355CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
title Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
spellingShingle Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
Bonin, Luka
PLANT
SUPERCRITICAL CARBON DIOXIDE
WATER-ETHANOL
EXTRACTS
POLYPHENOLS
BIOLOGICALLY ACTIVE COMPOUNDS
MEDICINE
title_short Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
title_full Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
title_fullStr Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
title_full_unstemmed Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
title_sort Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
dc.creator.none.fl_str_mv Bonin, Luka
Schofs, Laureano
Pečan, Luka Irenej
Oblak, Tine
Tavčar, Gaper
Ponikvar Svet, Maja
Silverman, Julian R.
Sanchez Bruni, Sergio Fabian
Jeran, Marko
author Bonin, Luka
author_facet Bonin, Luka
Schofs, Laureano
Pečan, Luka Irenej
Oblak, Tine
Tavčar, Gaper
Ponikvar Svet, Maja
Silverman, Julian R.
Sanchez Bruni, Sergio Fabian
Jeran, Marko
author_role author
author2 Schofs, Laureano
Pečan, Luka Irenej
Oblak, Tine
Tavčar, Gaper
Ponikvar Svet, Maja
Silverman, Julian R.
Sanchez Bruni, Sergio Fabian
Jeran, Marko
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLANT
SUPERCRITICAL CARBON DIOXIDE
WATER-ETHANOL
EXTRACTS
POLYPHENOLS
BIOLOGICALLY ACTIVE COMPOUNDS
MEDICINE
topic PLANT
SUPERCRITICAL CARBON DIOXIDE
WATER-ETHANOL
EXTRACTS
POLYPHENOLS
BIOLOGICALLY ACTIVE COMPOUNDS
MEDICINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes.
Fil: Bonin, Luka. University Of Ljubljana; Eslovenia
Fil: Schofs, Laureano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Pečan, Luka Irenej. University Of Ljubljana; Eslovenia. Università degli Studi di Trieste; Italia
Fil: Oblak, Tine. Institut Jožef Stefan; Eslovenia
Fil: Tavčar, Gaper. Institut Jožef Stefan; Eslovenia
Fil: Ponikvar Svet, Maja. Institut Jožef Stefan; Eslovenia
Fil: Silverman, Julian R.. Fashion Institute Of Technology; Estados Unidos
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Jeran, Marko. Institut Jožef Stefan; Eslovenia
Socratic Lectures: 12th International Symposium
Ljubljana
Eslovenia
University of Ljubljana. Faculty of Health Sciences
description Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268277
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture; Socratic Lectures: 12th International Symposium; Ljubljana; Eslovenia; 2025; 51-58
978-961-297-551-7
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268277
identifier_str_mv Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture; Socratic Lectures: 12th International Symposium; Ljubljana; Eslovenia; 2025; 51-58
978-961-297-551-7
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.55295/PSL.12.2025.II5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv University of Ljubljana. Faculty of Health Sciences
publisher.none.fl_str_mv University of Ljubljana. Faculty of Health Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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