Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture
- Autores
- Bonin, Luka; Schofs, Laureano; Pečan, Luka Irenej; Oblak, Tine; Tavčar, Gaper; Ponikvar Svet, Maja; Silverman, Julian R.; Sanchez Bruni, Sergio Fabian; Jeran, Marko
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes.
Fil: Bonin, Luka. University Of Ljubljana; Eslovenia
Fil: Schofs, Laureano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Pečan, Luka Irenej. University Of Ljubljana; Eslovenia. Università degli Studi di Trieste; Italia
Fil: Oblak, Tine. Institut Jožef Stefan; Eslovenia
Fil: Tavčar, Gaper. Institut Jožef Stefan; Eslovenia
Fil: Ponikvar Svet, Maja. Institut Jožef Stefan; Eslovenia
Fil: Silverman, Julian R.. Fashion Institute Of Technology; Estados Unidos
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Jeran, Marko. Institut Jožef Stefan; Eslovenia
Socratic Lectures: 12th International Symposium
Ljubljana
Eslovenia
University of Ljubljana. Faculty of Health Sciences - Materia
-
PLANT
SUPERCRITICAL CARBON DIOXIDE
WATER-ETHANOL
EXTRACTS
POLYPHENOLS
BIOLOGICALLY ACTIVE COMPOUNDS
MEDICINE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/268277
Ver los metadatos del registro completo
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Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixtureBonin, LukaSchofs, LaureanoPečan, Luka IrenejOblak, TineTavčar, GaperPonikvar Svet, MajaSilverman, Julian R.Sanchez Bruni, Sergio FabianJeran, MarkoPLANTSUPERCRITICAL CARBON DIOXIDEWATER-ETHANOLEXTRACTSPOLYPHENOLSBIOLOGICALLY ACTIVE COMPOUNDSMEDICINEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes.Fil: Bonin, Luka. University Of Ljubljana; EsloveniaFil: Schofs, Laureano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Pečan, Luka Irenej. University Of Ljubljana; Eslovenia. Università degli Studi di Trieste; ItaliaFil: Oblak, Tine. Institut Jožef Stefan; EsloveniaFil: Tavčar, Gaper. Institut Jožef Stefan; EsloveniaFil: Ponikvar Svet, Maja. Institut Jožef Stefan; EsloveniaFil: Silverman, Julian R.. Fashion Institute Of Technology; Estados UnidosFil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Jeran, Marko. Institut Jožef Stefan; EsloveniaSocratic Lectures: 12th International SymposiumLjubljanaEsloveniaUniversity of Ljubljana. Faculty of Health SciencesUniversity of Ljubljana. Faculty of Health Sciences2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268277Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture; Socratic Lectures: 12th International Symposium; Ljubljana; Eslovenia; 2025; 51-58978-961-297-551-7CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ebooks.uni-lj.si/ZalozbaUL/catalog/book/742info:eu-repo/semantics/altIdentifier/doi/10.55295/PSL.12.2025.II5Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:52Zoai:ri.conicet.gov.ar:11336/268277instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:52.355CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture |
title |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture |
spellingShingle |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture Bonin, Luka PLANT SUPERCRITICAL CARBON DIOXIDE WATER-ETHANOL EXTRACTS POLYPHENOLS BIOLOGICALLY ACTIVE COMPOUNDS MEDICINE |
title_short |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture |
title_full |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture |
title_fullStr |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture |
title_full_unstemmed |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture |
title_sort |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture |
dc.creator.none.fl_str_mv |
Bonin, Luka Schofs, Laureano Pečan, Luka Irenej Oblak, Tine Tavčar, Gaper Ponikvar Svet, Maja Silverman, Julian R. Sanchez Bruni, Sergio Fabian Jeran, Marko |
author |
Bonin, Luka |
author_facet |
Bonin, Luka Schofs, Laureano Pečan, Luka Irenej Oblak, Tine Tavčar, Gaper Ponikvar Svet, Maja Silverman, Julian R. Sanchez Bruni, Sergio Fabian Jeran, Marko |
author_role |
author |
author2 |
Schofs, Laureano Pečan, Luka Irenej Oblak, Tine Tavčar, Gaper Ponikvar Svet, Maja Silverman, Julian R. Sanchez Bruni, Sergio Fabian Jeran, Marko |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
PLANT SUPERCRITICAL CARBON DIOXIDE WATER-ETHANOL EXTRACTS POLYPHENOLS BIOLOGICALLY ACTIVE COMPOUNDS MEDICINE |
topic |
PLANT SUPERCRITICAL CARBON DIOXIDE WATER-ETHANOL EXTRACTS POLYPHENOLS BIOLOGICALLY ACTIVE COMPOUNDS MEDICINE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes. Fil: Bonin, Luka. University Of Ljubljana; Eslovenia Fil: Schofs, Laureano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Pečan, Luka Irenej. University Of Ljubljana; Eslovenia. Università degli Studi di Trieste; Italia Fil: Oblak, Tine. Institut Jožef Stefan; Eslovenia Fil: Tavčar, Gaper. Institut Jožef Stefan; Eslovenia Fil: Ponikvar Svet, Maja. Institut Jožef Stefan; Eslovenia Fil: Silverman, Julian R.. Fashion Institute Of Technology; Estados Unidos Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Jeran, Marko. Institut Jožef Stefan; Eslovenia Socratic Lectures: 12th International Symposium Ljubljana Eslovenia University of Ljubljana. Faculty of Health Sciences |
description |
Natural products serve as an important source of bioactive compounds with significant applications in various fields of science. Among these, polyphenols are particularly noteworthy due to their antimicrobial and antioxidant properties and role in capturing free radicals. This study quantitatively determined the total phenolic content in plant extracts prepared using two distinct extraction methods: supercritical carbon dioxide (SC-CO₂) and water-ethanol maceration. The Folin-Ciocalteu method was employed for quantitative analysis, with results expressed as gallic acid equivalents per kilogram of plant material. The findings demonstrated that water-ethanol extracts consistently contained higher levels of phenolic compounds compared to SC-CO₂ extracts across all analyzed samples. For instance, rosemary extracts prepared with water-ethanol had the highest phenolic content, while thyme and marigold showed lower values. In contrast, SC-CO₂ extracts yielded significantly lower phenolic concentrations, likely due to the selective extraction of lipophilic compounds. Variations in phenolic content were attributed to factors such as sample polarity, plant type, and environmental conditions. These results highlight the importance of selecting appropriate extraction methods based on the desired application. Water-ethanol maceration proved superior for obtaining extracts rich in phenolic compounds, making it suitable for applications requiring high antioxidant activity. This study underscores the potential of plant-derived polyphenols as natural, "green" bioactive ingredients for diverse scientific and therapeutic purposes. |
publishDate |
2025 |
dc.date.none.fl_str_mv |
2025 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Simposio Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/268277 Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture; Socratic Lectures: 12th International Symposium; Ljubljana; Eslovenia; 2025; 51-58 978-961-297-551-7 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/268277 |
identifier_str_mv |
Quantitative determination of total phenolic compounds in plant extracts using supercritical carbon dioxide and water-ethanol mixture; Socratic Lectures: 12th International Symposium; Ljubljana; Eslovenia; 2025; 51-58 978-961-297-551-7 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by/2.5/ar/ |
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dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
University of Ljubljana. Faculty of Health Sciences |
publisher.none.fl_str_mv |
University of Ljubljana. Faculty of Health Sciences |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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