Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
- Autores
- Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; Colman Lerner, Alejandro Ariel
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment.
Fil: Baltanas, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Hohmann, Stefan. University of Gothenburg. Department of Cell and Molecular Biology; Suecia
Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina - Materia
-
Signal Transdunction
Mapk
Stress
Cross-Talk - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2164
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Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathwayBaltanas, RodrigoBush, AlanCouto, Alicia SusanaDurrieu, LucíaHohmann, StefanColman Lerner, Alejandro ArielSignal TransdunctionMapkStressCross-Talkhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment.Fil: Baltanas, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Hohmann, Stefan. University of Gothenburg. Department of Cell and Molecular Biology; SueciaFil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaAmerican Association for the Advancement of Science2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2164Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; et al.; Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway; American Association for the Advancement of Science; Science Signaling; 6; 272; 4-2013; ra261937-9145enginfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1126/scisignal.2003312info:eu-repo/semantics/altIdentifier/url/http://stke.sciencemag.org/content/6/272/ra26info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:12Zoai:ri.conicet.gov.ar:11336/2164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:13.112CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
title |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
spellingShingle |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway Baltanas, Rodrigo Signal Transdunction Mapk Stress Cross-Talk |
title_short |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
title_full |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
title_fullStr |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
title_full_unstemmed |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
title_sort |
Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
dc.creator.none.fl_str_mv |
Baltanas, Rodrigo Bush, Alan Couto, Alicia Susana Durrieu, Lucía Hohmann, Stefan Colman Lerner, Alejandro Ariel |
author |
Baltanas, Rodrigo |
author_facet |
Baltanas, Rodrigo Bush, Alan Couto, Alicia Susana Durrieu, Lucía Hohmann, Stefan Colman Lerner, Alejandro Ariel |
author_role |
author |
author2 |
Bush, Alan Couto, Alicia Susana Durrieu, Lucía Hohmann, Stefan Colman Lerner, Alejandro Ariel |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Signal Transdunction Mapk Stress Cross-Talk |
topic |
Signal Transdunction Mapk Stress Cross-Talk |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment. Fil: Baltanas, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Hohmann, Stefan. University of Gothenburg. Department of Cell and Molecular Biology; Suecia Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina |
description |
Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2164 Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; et al.; Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway; American Association for the Advancement of Science; Science Signaling; 6; 272; 4-2013; ra26 1937-9145 |
url |
http://hdl.handle.net/11336/2164 |
identifier_str_mv |
Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; et al.; Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway; American Association for the Advancement of Science; Science Signaling; 6; 272; 4-2013; ra26 1937-9145 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1126/scisignal.2003312 info:eu-repo/semantics/altIdentifier/url/http://stke.sciencemag.org/content/6/272/ra26 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Association for the Advancement of Science |
publisher.none.fl_str_mv |
American Association for the Advancement of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |