Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway

Autores
Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; Colman Lerner, Alejandro Ariel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment.
Fil: Baltanas, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Hohmann, Stefan. University of Gothenburg. Department of Cell and Molecular Biology; Suecia
Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
Signal Transdunction
Mapk
Stress
Cross-Talk
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2164
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathwayBaltanas, RodrigoBush, AlanCouto, Alicia SusanaDurrieu, LucíaHohmann, StefanColman Lerner, Alejandro ArielSignal TransdunctionMapkStressCross-Talkhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment.Fil: Baltanas, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Hohmann, Stefan. University of Gothenburg. Department of Cell and Molecular Biology; SueciaFil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaAmerican Association for the Advancement of Science2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2164Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; et al.; Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway; American Association for the Advancement of Science; Science Signaling; 6; 272; 4-2013; ra261937-9145enginfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1126/scisignal.2003312info:eu-repo/semantics/altIdentifier/url/http://stke.sciencemag.org/content/6/272/ra26info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:03:39Zoai:ri.conicet.gov.ar:11336/2164instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:03:39.297CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
title Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
spellingShingle Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
Baltanas, Rodrigo
Signal Transdunction
Mapk
Stress
Cross-Talk
title_short Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
title_full Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
title_fullStr Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
title_full_unstemmed Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
title_sort Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway
dc.creator.none.fl_str_mv Baltanas, Rodrigo
Bush, Alan
Couto, Alicia Susana
Durrieu, Lucía
Hohmann, Stefan
Colman Lerner, Alejandro Ariel
author Baltanas, Rodrigo
author_facet Baltanas, Rodrigo
Bush, Alan
Couto, Alicia Susana
Durrieu, Lucía
Hohmann, Stefan
Colman Lerner, Alejandro Ariel
author_role author
author2 Bush, Alan
Couto, Alicia Susana
Durrieu, Lucía
Hohmann, Stefan
Colman Lerner, Alejandro Ariel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Signal Transdunction
Mapk
Stress
Cross-Talk
topic Signal Transdunction
Mapk
Stress
Cross-Talk
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment.
Fil: Baltanas, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bush, Alan. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Couto, Alicia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Durrieu, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Hohmann, Stefan. University of Gothenburg. Department of Cell and Molecular Biology; Suecia
Fil: Colman Lerner, Alejandro Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas . Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the "shmooing" morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2164
Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; et al.; Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway; American Association for the Advancement of Science; Science Signaling; 6; 272; 4-2013; ra26
1937-9145
url http://hdl.handle.net/11336/2164
identifier_str_mv Baltanas, Rodrigo; Bush, Alan; Couto, Alicia Susana; Durrieu, Lucía; Hohmann, Stefan; et al.; Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway; American Association for the Advancement of Science; Science Signaling; 6; 272; 4-2013; ra26
1937-9145
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/DOI:10.1126/scisignal.2003312
info:eu-repo/semantics/altIdentifier/url/http://stke.sciencemag.org/content/6/272/ra26
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association for the Advancement of Science
publisher.none.fl_str_mv American Association for the Advancement of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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