Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
- Autores
- Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target.
Fil: Natera, Jose Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Massad, Walter Alfredo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
THYAMINE
RIBOFLAVIN
PHOTOOXDATION
ANTIOXIDANT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/189553
Ver los metadatos del registro completo
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Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2Natera, Jose EduardoMassad, Walter AlfredoGarcia, Norman AndinoTHYAMINERIBOFLAVINPHOTOOXDATIONANTIOXIDANThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target.Fil: Natera, Jose Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Massad, Walter Alfredo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189553Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino; Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 87; 2; 3-2011; 317-3230031-86551751-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00867.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:38Zoai:ri.conicet.gov.ar:11336/189553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:38.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 |
title |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 |
spellingShingle |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 Natera, Jose Eduardo THYAMINE RIBOFLAVIN PHOTOOXDATION ANTIOXIDANT |
title_short |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 |
title_full |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 |
title_fullStr |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 |
title_full_unstemmed |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 |
title_sort |
Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2 |
dc.creator.none.fl_str_mv |
Natera, Jose Eduardo Massad, Walter Alfredo Garcia, Norman Andino |
author |
Natera, Jose Eduardo |
author_facet |
Natera, Jose Eduardo Massad, Walter Alfredo Garcia, Norman Andino |
author_role |
author |
author2 |
Massad, Walter Alfredo Garcia, Norman Andino |
author2_role |
author author |
dc.subject.none.fl_str_mv |
THYAMINE RIBOFLAVIN PHOTOOXDATION ANTIOXIDANT |
topic |
THYAMINE RIBOFLAVIN PHOTOOXDATION ANTIOXIDANT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target. Fil: Natera, Jose Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina Fil: Massad, Walter Alfredo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina Fil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/189553 Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino; Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 87; 2; 3-2011; 317-323 0031-8655 1751-1097 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/189553 |
identifier_str_mv |
Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino; Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 87; 2; 3-2011; 317-323 0031-8655 1751-1097 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00867.x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846082584781520896 |
score |
13.22299 |