Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2

Autores
Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target.
Fil: Natera, Jose Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Massad, Walter Alfredo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
THYAMINE
RIBOFLAVIN
PHOTOOXDATION
ANTIOXIDANT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189553

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spelling Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2Natera, Jose EduardoMassad, Walter AlfredoGarcia, Norman AndinoTHYAMINERIBOFLAVINPHOTOOXDATIONANTIOXIDANThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target.Fil: Natera, Jose Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Massad, Walter Alfredo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189553Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino; Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 87; 2; 3-2011; 317-3230031-86551751-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00867.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:38Zoai:ri.conicet.gov.ar:11336/189553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:38.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
title Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
spellingShingle Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
Natera, Jose Eduardo
THYAMINE
RIBOFLAVIN
PHOTOOXDATION
ANTIOXIDANT
title_short Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
title_full Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
title_fullStr Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
title_full_unstemmed Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
title_sort Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2
dc.creator.none.fl_str_mv Natera, Jose Eduardo
Massad, Walter Alfredo
Garcia, Norman Andino
author Natera, Jose Eduardo
author_facet Natera, Jose Eduardo
Massad, Walter Alfredo
Garcia, Norman Andino
author_role author
author2 Massad, Walter Alfredo
Garcia, Norman Andino
author2_role author
author
dc.subject.none.fl_str_mv THYAMINE
RIBOFLAVIN
PHOTOOXDATION
ANTIOXIDANT
topic THYAMINE
RIBOFLAVIN
PHOTOOXDATION
ANTIOXIDANT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target.
Fil: Natera, Jose Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Massad, Walter Alfredo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Garcia, Norman Andino. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Kinetics and mechanism of photoprocesses generated by visible light-irradiation of the system riboflavin (Rf, vitamin B2) plus Thiamine (Th) and Thiamine pyrophosphate (ThDP), representing vitamin B1, was studied in pH 7 water. A weak dark complex vitamin B2-vitamin B1, with a mean value of 4 ± 0.4 m-1 is formed. An intricate mechanism of competitive reactions operates upon photoirradiation, being the light only absorbed by Rf. Th and ThDP quench excited singlet and triplet states of Rf, with rate constants in the order of 109 and 106m-1s-1, respectively. With Vitamin B1 in a concentration similar to that of dissolved molecular oxygen in water, the quenching of triplet excited Rf by the latter is highly predominant, resulting in the generation of O2( 1Δg). Superoxide radical anion was not detected under work conditions. A relatively slow O2(1Δ g)-mediated photodegradation of Th and ThDP was observed. Nevertheless, Th and especially ThDP behave as efficient physical deactivators of O2(1Δg). The thiazol structure in vitamin B1 appears as a good scavenger of this reactive oxygen species. This characteristic, that presents at vitamin B1 as a potential photoprotector of biological entities against O2(1Δg) attack, was been experimentally confirmed employing the protein lisozime as a photo-oxidizable target.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189553
Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino; Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 87; 2; 3-2011; 317-323
0031-8655
1751-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189553
identifier_str_mv Natera, Jose Eduardo; Massad, Walter Alfredo; Garcia, Norman Andino; Vitamin B1 as a scavenger of reactive oxygen species photogenerated by vitamin B2; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 87; 2; 3-2011; 317-323
0031-8655
1751-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1751-1097.2010.00867.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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