In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry

Autores
Cabruja, Matias Ezequiel; Priotti, Josefina; Domizi, Pablo Daniel; Papsdorf, Katharina; Kroetz, Deanna L.; Brunet, Anne; Contrepois, Kévin; Snyder, Michael P.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Total triacylglycerol (TAG) level is a key clinical marker of metabolic and cardiovascular diseases. However, the roles of individual TAGs have not been thoroughly explored in part due to their extreme structural complexity. We present a targeted mass spectrometry-based method combining multiple reaction monitoring (MRM) and multiple stage mass spectrometry (MS3) for the comprehensive qualitative and semiquantitative profiling of TAGs. This method referred as TriP-MS3 – triacylglycerol profiling using MS3 – screens for more than 6,700 TAG species in a fully automated fashion. TriP-MS3 demonstrated excellent reproducibility (median interday CV ∼ 0.15) and linearity (median R2 = 0.978) and detected 285 individual TAG species in human plasma. The semiquantitative accuracy of the method was validated by comparison with a state-of-the-art reverse phase liquid chromatography (RPLC)-MS (R2 = 0.83), which is the most commonly used approach for TAGs profiling. Finally, we demonstrate the utility and the versatility of the method by characterizing the effects of a fatty acid desaturase inhibitor on TAG profiles in vitro and by profiling TAGs in Caenorhabditis elegans.
Fil: Cabruja, Matias Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of Stanford; Estados Unidos
Fil: Priotti, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of California; Estados Unidos
Fil: Domizi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of Stanford; Estados Unidos
Fil: Papsdorf, Katharina. University of Stanford; Estados Unidos
Fil: Kroetz, Deanna L.. University of California; Estados Unidos
Fil: Brunet, Anne. University of Stanford; Estados Unidos
Fil: Contrepois, Kévin. University of Stanford; Estados Unidos
Fil: Snyder, Michael P.. University of Stanford; Estados Unidos
Materia
FATTY ACID
LIPIDS
MASS SPECTROMETRY
TRIACYLGLYCEROLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153310
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometryCabruja, Matias EzequielPriotti, JosefinaDomizi, Pablo DanielPapsdorf, KatharinaKroetz, Deanna L.Brunet, AnneContrepois, KévinSnyder, Michael P.FATTY ACIDLIPIDSMASS SPECTROMETRYTRIACYLGLYCEROLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Total triacylglycerol (TAG) level is a key clinical marker of metabolic and cardiovascular diseases. However, the roles of individual TAGs have not been thoroughly explored in part due to their extreme structural complexity. We present a targeted mass spectrometry-based method combining multiple reaction monitoring (MRM) and multiple stage mass spectrometry (MS3) for the comprehensive qualitative and semiquantitative profiling of TAGs. This method referred as TriP-MS3 – triacylglycerol profiling using MS3 – screens for more than 6,700 TAG species in a fully automated fashion. TriP-MS3 demonstrated excellent reproducibility (median interday CV ∼ 0.15) and linearity (median R2 = 0.978) and detected 285 individual TAG species in human plasma. The semiquantitative accuracy of the method was validated by comparison with a state-of-the-art reverse phase liquid chromatography (RPLC)-MS (R2 = 0.83), which is the most commonly used approach for TAGs profiling. Finally, we demonstrate the utility and the versatility of the method by characterizing the effects of a fatty acid desaturase inhibitor on TAG profiles in vitro and by profiling TAGs in Caenorhabditis elegans.Fil: Cabruja, Matias Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of Stanford; Estados UnidosFil: Priotti, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of California; Estados UnidosFil: Domizi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of Stanford; Estados UnidosFil: Papsdorf, Katharina. University of Stanford; Estados UnidosFil: Kroetz, Deanna L.. University of California; Estados UnidosFil: Brunet, Anne. University of Stanford; Estados UnidosFil: Contrepois, Kévin. University of Stanford; Estados UnidosFil: Snyder, Michael P.. University of Stanford; Estados UnidosElsevier Science2021-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153310Cabruja, Matias Ezequiel; Priotti, Josefina; Domizi, Pablo Daniel; Papsdorf, Katharina; Kroetz, Deanna L.; et al.; In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry; Elsevier Science; Analytica Chimica Acta; 1184; 1-11-2021; 1-90003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267021008497info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2021.339023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:02:49Zoai:ri.conicet.gov.ar:11336/153310instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:02:50.131CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
title In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
spellingShingle In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
Cabruja, Matias Ezequiel
FATTY ACID
LIPIDS
MASS SPECTROMETRY
TRIACYLGLYCEROLS
title_short In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
title_full In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
title_fullStr In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
title_full_unstemmed In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
title_sort In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry
dc.creator.none.fl_str_mv Cabruja, Matias Ezequiel
Priotti, Josefina
Domizi, Pablo Daniel
Papsdorf, Katharina
Kroetz, Deanna L.
Brunet, Anne
Contrepois, Kévin
Snyder, Michael P.
author Cabruja, Matias Ezequiel
author_facet Cabruja, Matias Ezequiel
Priotti, Josefina
Domizi, Pablo Daniel
Papsdorf, Katharina
Kroetz, Deanna L.
Brunet, Anne
Contrepois, Kévin
Snyder, Michael P.
author_role author
author2 Priotti, Josefina
Domizi, Pablo Daniel
Papsdorf, Katharina
Kroetz, Deanna L.
Brunet, Anne
Contrepois, Kévin
Snyder, Michael P.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FATTY ACID
LIPIDS
MASS SPECTROMETRY
TRIACYLGLYCEROLS
topic FATTY ACID
LIPIDS
MASS SPECTROMETRY
TRIACYLGLYCEROLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Total triacylglycerol (TAG) level is a key clinical marker of metabolic and cardiovascular diseases. However, the roles of individual TAGs have not been thoroughly explored in part due to their extreme structural complexity. We present a targeted mass spectrometry-based method combining multiple reaction monitoring (MRM) and multiple stage mass spectrometry (MS3) for the comprehensive qualitative and semiquantitative profiling of TAGs. This method referred as TriP-MS3 – triacylglycerol profiling using MS3 – screens for more than 6,700 TAG species in a fully automated fashion. TriP-MS3 demonstrated excellent reproducibility (median interday CV ∼ 0.15) and linearity (median R2 = 0.978) and detected 285 individual TAG species in human plasma. The semiquantitative accuracy of the method was validated by comparison with a state-of-the-art reverse phase liquid chromatography (RPLC)-MS (R2 = 0.83), which is the most commonly used approach for TAGs profiling. Finally, we demonstrate the utility and the versatility of the method by characterizing the effects of a fatty acid desaturase inhibitor on TAG profiles in vitro and by profiling TAGs in Caenorhabditis elegans.
Fil: Cabruja, Matias Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of Stanford; Estados Unidos
Fil: Priotti, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of California; Estados Unidos
Fil: Domizi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. University of Stanford; Estados Unidos
Fil: Papsdorf, Katharina. University of Stanford; Estados Unidos
Fil: Kroetz, Deanna L.. University of California; Estados Unidos
Fil: Brunet, Anne. University of Stanford; Estados Unidos
Fil: Contrepois, Kévin. University of Stanford; Estados Unidos
Fil: Snyder, Michael P.. University of Stanford; Estados Unidos
description Total triacylglycerol (TAG) level is a key clinical marker of metabolic and cardiovascular diseases. However, the roles of individual TAGs have not been thoroughly explored in part due to their extreme structural complexity. We present a targeted mass spectrometry-based method combining multiple reaction monitoring (MRM) and multiple stage mass spectrometry (MS3) for the comprehensive qualitative and semiquantitative profiling of TAGs. This method referred as TriP-MS3 – triacylglycerol profiling using MS3 – screens for more than 6,700 TAG species in a fully automated fashion. TriP-MS3 demonstrated excellent reproducibility (median interday CV ∼ 0.15) and linearity (median R2 = 0.978) and detected 285 individual TAG species in human plasma. The semiquantitative accuracy of the method was validated by comparison with a state-of-the-art reverse phase liquid chromatography (RPLC)-MS (R2 = 0.83), which is the most commonly used approach for TAGs profiling. Finally, we demonstrate the utility and the versatility of the method by characterizing the effects of a fatty acid desaturase inhibitor on TAG profiles in vitro and by profiling TAGs in Caenorhabditis elegans.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153310
Cabruja, Matias Ezequiel; Priotti, Josefina; Domizi, Pablo Daniel; Papsdorf, Katharina; Kroetz, Deanna L.; et al.; In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry; Elsevier Science; Analytica Chimica Acta; 1184; 1-11-2021; 1-9
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153310
identifier_str_mv Cabruja, Matias Ezequiel; Priotti, Josefina; Domizi, Pablo Daniel; Papsdorf, Katharina; Kroetz, Deanna L.; et al.; In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry; Elsevier Science; Analytica Chimica Acta; 1184; 1-11-2021; 1-9
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267021008497
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2021.339023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.121305

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