B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis

Autores
Parodi Ramoneda, Cecilia María; Barrio, Alejandra; Garcia Bustos, Maria Fernanda; González Prieto, Ana Gabriela; Pimentel Solá, María Julia; Badano, Maria Noel; Albareda, María Cecilia; Castro Eiro, Melisa Daiana; Laucella, Susana Adriana; Elizalde de Bracco, María Marta
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: The aim of this study was to evaluate characteristics of B cells in human tegumentary leismaniasis (TL) analysing cutaneous leishmaniasis (CL), most prevalent form and mucosal leishmaniasis (ML), aggressive form characterized by the destruction of the oral-nasal-pharyngeal cavities. Methods and results: By flow cytometry analysis, we found decreased percentages of non–class-switched memory B cells in TL with the degree of the loss related to clinical severity. Using commercial ELISA, we reported high levels of B-cell activating factor (BAFF) and IgG preferentially in aggressive CL and markedly in ML together with decreased BAFF receptors in the latter. We also found lower levels of BAFF after clinical recovery suggesting a relation between BAFF and disease activity. Mucosal leishmaniasis history of therapeutic failure presented high levels of BAFF accompanied by detectable concentrations of IFN-γ and IL-6 (assayed by commercial ELISA and cytometric bead arrays respectively), cytokines involved in exaggerated inflammatory responses and tissue damage in TL. Conclusion: We demonstrate B-cell disturbances in TL with the degree of the alterations related to clinical severity. We suggest a relation between excess of BAFF and disease activity and point towards a possible implication of BAFF in the inflammatory phenomenon of ML.
Fil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Barrio, Alejandra. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Cátedra de Microbiología; Argentina
Fil: Garcia Bustos, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: González Prieto, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Cátedra de Microbiología; Argentina
Fil: Pimentel Solá, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Badano, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Albareda, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Castro Eiro, Melisa Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Laucella, Susana Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Elizalde de Bracco, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
B LYMPHOCYTES
B-CELL ACTIVATING FACTOR
CUTANEOUS LEISHMANIASIS
HYPERGAMMAGLOBULINEMIA
INTERFERON-GAMMA
INTERLEUKIN-6
MUCOCUTANEOUS LEISHMANIASIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168880
Acceder
id CONICETDig_395dfab5c7b74fd1f778833f73a46bb5
oai_identifier_str oai:ri.conicet.gov.ar:11336/168880
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasisParodi Ramoneda, Cecilia MaríaBarrio, AlejandraGarcia Bustos, Maria FernandaGonzález Prieto, Ana GabrielaPimentel Solá, María JuliaBadano, Maria NoelAlbareda, María CeciliaCastro Eiro, Melisa DaianaLaucella, Susana AdrianaElizalde de Bracco, María MartaB LYMPHOCYTESB-CELL ACTIVATING FACTORCUTANEOUS LEISHMANIASISHYPERGAMMAGLOBULINEMIAINTERFERON-GAMMAINTERLEUKIN-6MUCOCUTANEOUS LEISHMANIASIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Aims: The aim of this study was to evaluate characteristics of B cells in human tegumentary leismaniasis (TL) analysing cutaneous leishmaniasis (CL), most prevalent form and mucosal leishmaniasis (ML), aggressive form characterized by the destruction of the oral-nasal-pharyngeal cavities. Methods and results: By flow cytometry analysis, we found decreased percentages of non–class-switched memory B cells in TL with the degree of the loss related to clinical severity. Using commercial ELISA, we reported high levels of B-cell activating factor (BAFF) and IgG preferentially in aggressive CL and markedly in ML together with decreased BAFF receptors in the latter. We also found lower levels of BAFF after clinical recovery suggesting a relation between BAFF and disease activity. Mucosal leishmaniasis history of therapeutic failure presented high levels of BAFF accompanied by detectable concentrations of IFN-γ and IL-6 (assayed by commercial ELISA and cytometric bead arrays respectively), cytokines involved in exaggerated inflammatory responses and tissue damage in TL. Conclusion: We demonstrate B-cell disturbances in TL with the degree of the alterations related to clinical severity. We suggest a relation between excess of BAFF and disease activity and point towards a possible implication of BAFF in the inflammatory phenomenon of ML.Fil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Barrio, Alejandra. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Cátedra de Microbiología; ArgentinaFil: Garcia Bustos, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: González Prieto, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Cátedra de Microbiología; ArgentinaFil: Pimentel Solá, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Badano, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Albareda, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Castro Eiro, Melisa Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Laucella, Susana Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Elizalde de Bracco, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaWiley2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168880Parodi Ramoneda, Cecilia María; Barrio, Alejandra; Garcia Bustos, Maria Fernanda; González Prieto, Ana Gabriela; Pimentel Solá, María Julia; et al.; B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis; Wiley; Parasite Immunology; 42; 9; 9-2020; 1-220141-9838CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/pim.12759info:eu-repo/semantics/altIdentifier/doi/10.1111/pim.12759info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:03:34Zoai:ri.conicet.gov.ar:11336/168880instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:03:34.674CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
title B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
spellingShingle B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
Parodi Ramoneda, Cecilia María
B LYMPHOCYTES
B-CELL ACTIVATING FACTOR
CUTANEOUS LEISHMANIASIS
HYPERGAMMAGLOBULINEMIA
INTERFERON-GAMMA
INTERLEUKIN-6
MUCOCUTANEOUS LEISHMANIASIS
title_short B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
title_full B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
title_fullStr B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
title_full_unstemmed B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
title_sort B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis
dc.creator.none.fl_str_mv Parodi Ramoneda, Cecilia María
Barrio, Alejandra
Garcia Bustos, Maria Fernanda
González Prieto, Ana Gabriela
Pimentel Solá, María Julia
Badano, Maria Noel
Albareda, María Cecilia
Castro Eiro, Melisa Daiana
Laucella, Susana Adriana
Elizalde de Bracco, María Marta
author Parodi Ramoneda, Cecilia María
author_facet Parodi Ramoneda, Cecilia María
Barrio, Alejandra
Garcia Bustos, Maria Fernanda
González Prieto, Ana Gabriela
Pimentel Solá, María Julia
Badano, Maria Noel
Albareda, María Cecilia
Castro Eiro, Melisa Daiana
Laucella, Susana Adriana
Elizalde de Bracco, María Marta
author_role author
author2 Barrio, Alejandra
Garcia Bustos, Maria Fernanda
González Prieto, Ana Gabriela
Pimentel Solá, María Julia
Badano, Maria Noel
Albareda, María Cecilia
Castro Eiro, Melisa Daiana
Laucella, Susana Adriana
Elizalde de Bracco, María Marta
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv B LYMPHOCYTES
B-CELL ACTIVATING FACTOR
CUTANEOUS LEISHMANIASIS
HYPERGAMMAGLOBULINEMIA
INTERFERON-GAMMA
INTERLEUKIN-6
MUCOCUTANEOUS LEISHMANIASIS
topic B LYMPHOCYTES
B-CELL ACTIVATING FACTOR
CUTANEOUS LEISHMANIASIS
HYPERGAMMAGLOBULINEMIA
INTERFERON-GAMMA
INTERLEUKIN-6
MUCOCUTANEOUS LEISHMANIASIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aims: The aim of this study was to evaluate characteristics of B cells in human tegumentary leismaniasis (TL) analysing cutaneous leishmaniasis (CL), most prevalent form and mucosal leishmaniasis (ML), aggressive form characterized by the destruction of the oral-nasal-pharyngeal cavities. Methods and results: By flow cytometry analysis, we found decreased percentages of non–class-switched memory B cells in TL with the degree of the loss related to clinical severity. Using commercial ELISA, we reported high levels of B-cell activating factor (BAFF) and IgG preferentially in aggressive CL and markedly in ML together with decreased BAFF receptors in the latter. We also found lower levels of BAFF after clinical recovery suggesting a relation between BAFF and disease activity. Mucosal leishmaniasis history of therapeutic failure presented high levels of BAFF accompanied by detectable concentrations of IFN-γ and IL-6 (assayed by commercial ELISA and cytometric bead arrays respectively), cytokines involved in exaggerated inflammatory responses and tissue damage in TL. Conclusion: We demonstrate B-cell disturbances in TL with the degree of the alterations related to clinical severity. We suggest a relation between excess of BAFF and disease activity and point towards a possible implication of BAFF in the inflammatory phenomenon of ML.
Fil: Parodi Ramoneda, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Barrio, Alejandra. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Cátedra de Microbiología; Argentina
Fil: Garcia Bustos, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: González Prieto, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Cs.de la Salud. Cátedra de Microbiología; Argentina
Fil: Pimentel Solá, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Badano, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Albareda, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Castro Eiro, Melisa Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Laucella, Susana Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina
Fil: Elizalde de Bracco, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description Aims: The aim of this study was to evaluate characteristics of B cells in human tegumentary leismaniasis (TL) analysing cutaneous leishmaniasis (CL), most prevalent form and mucosal leishmaniasis (ML), aggressive form characterized by the destruction of the oral-nasal-pharyngeal cavities. Methods and results: By flow cytometry analysis, we found decreased percentages of non–class-switched memory B cells in TL with the degree of the loss related to clinical severity. Using commercial ELISA, we reported high levels of B-cell activating factor (BAFF) and IgG preferentially in aggressive CL and markedly in ML together with decreased BAFF receptors in the latter. We also found lower levels of BAFF after clinical recovery suggesting a relation between BAFF and disease activity. Mucosal leishmaniasis history of therapeutic failure presented high levels of BAFF accompanied by detectable concentrations of IFN-γ and IL-6 (assayed by commercial ELISA and cytometric bead arrays respectively), cytokines involved in exaggerated inflammatory responses and tissue damage in TL. Conclusion: We demonstrate B-cell disturbances in TL with the degree of the alterations related to clinical severity. We suggest a relation between excess of BAFF and disease activity and point towards a possible implication of BAFF in the inflammatory phenomenon of ML.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168880
Parodi Ramoneda, Cecilia María; Barrio, Alejandra; Garcia Bustos, Maria Fernanda; González Prieto, Ana Gabriela; Pimentel Solá, María Julia; et al.; B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis; Wiley; Parasite Immunology; 42; 9; 9-2020; 1-22
0141-9838
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168880
identifier_str_mv Parodi Ramoneda, Cecilia María; Barrio, Alejandra; Garcia Bustos, Maria Fernanda; González Prieto, Ana Gabriela; Pimentel Solá, María Julia; et al.; B-cell profile, B-cell activating factor concentration and IgG levels in human cutaneous and mucosal leishmaniasis; Wiley; Parasite Immunology; 42; 9; 9-2020; 1-22
0141-9838
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/pim.12759
info:eu-repo/semantics/altIdentifier/doi/10.1111/pim.12759
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980092521742336
score 12.993085

Publicaciones similares