Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease

Autores
Albareda, María Cecilia; Pérez Mazliah, Damián Eduardo; Natale, Maria Ailen; Castro Eiro, Melisa Daiana; Alvarez, María G.; Viotti, Rodolfo Jorge; Bertocchi, Graciela; Lococo, Bruno; Tarleton, Rick L.; Laucella, Susana Adriana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have previously demonstrated that immune responses in subjects with chronic Trypanosoma cruzi infection display features common to other persistent infections with signs of T cell exhaustion. Alterations in cytokine receptor signal transduction have emerged as one of the cell-intrinsic mechanisms of T cell exhaustion. In this study, we performed an analysis of the expression of IL-7R components (CD127 and CD132) on CD4+ and CD8+ T cells and evaluated IL-7–dependent signaling events in patients at different clinical stages of chronic chagasic heart disease. Subjects with no signs of cardiac disease showed a decrease in CD127+CD132+ cells and a reciprocal gain of CD127−CD132+ in CD8+ and CD4+ T cells compared with either patients exhibiting heart enlargement or uninfected controls. T. cruzi infection, in vitro, was able to stimulate the downregulation of CD127 and the upregulation of CD132 on T cells. IL-7–induced phosphorylation of STAT5 as well as Bcl-2 and CD25 expression were lower in T. cruzi–infected subjects compared with uninfected controls. The serum levels of IL-7 were also increased in chronic chagasic patients. The present study highlights perturbed IL-7/IL-7R T cell signaling through STAT5 as a potential mechanism of T cell exhaustion in chronic T. cruzi infection.
Fil: Albareda, María Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Mazliah, Damián Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
Fil: Natale, Maria Ailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
Fil: Castro Eiro, Melisa Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
Fil: Alvarez, María G.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Viotti, Rodolfo Jorge. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Bertocchi, Graciela. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Lococo, Bruno. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Tarleton, Rick L.. Center for Tropical and Emerging Global Diseases; Grecia
Fil: Laucella, Susana Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
Materia
T Cruzi
Chagas Disease
Il7 Receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38456
Acceder
id CONICETDig_38e0cbdd49d64975508fa694b5578ff9
oai_identifier_str oai:ri.conicet.gov.ar:11336/38456
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas DiseaseAlbareda, María CeciliaPérez Mazliah, Damián EduardoNatale, Maria AilenCastro Eiro, Melisa DaianaAlvarez, María G.Viotti, Rodolfo JorgeBertocchi, GracielaLococo, BrunoTarleton, Rick L.Laucella, Susana AdrianaT CruziChagas DiseaseIl7 Receptorhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3We have previously demonstrated that immune responses in subjects with chronic Trypanosoma cruzi infection display features common to other persistent infections with signs of T cell exhaustion. Alterations in cytokine receptor signal transduction have emerged as one of the cell-intrinsic mechanisms of T cell exhaustion. In this study, we performed an analysis of the expression of IL-7R components (CD127 and CD132) on CD4+ and CD8+ T cells and evaluated IL-7–dependent signaling events in patients at different clinical stages of chronic chagasic heart disease. Subjects with no signs of cardiac disease showed a decrease in CD127+CD132+ cells and a reciprocal gain of CD127−CD132+ in CD8+ and CD4+ T cells compared with either patients exhibiting heart enlargement or uninfected controls. T. cruzi infection, in vitro, was able to stimulate the downregulation of CD127 and the upregulation of CD132 on T cells. IL-7–induced phosphorylation of STAT5 as well as Bcl-2 and CD25 expression were lower in T. cruzi–infected subjects compared with uninfected controls. The serum levels of IL-7 were also increased in chronic chagasic patients. The present study highlights perturbed IL-7/IL-7R T cell signaling through STAT5 as a potential mechanism of T cell exhaustion in chronic T. cruzi infection.Fil: Albareda, María Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez Mazliah, Damián Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; ArgentinaFil: Natale, Maria Ailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; ArgentinaFil: Castro Eiro, Melisa Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; ArgentinaFil: Alvarez, María G.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; ArgentinaFil: Viotti, Rodolfo Jorge. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; ArgentinaFil: Bertocchi, Graciela. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; ArgentinaFil: Lococo, Bruno. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; ArgentinaFil: Tarleton, Rick L.. Center for Tropical and Emerging Global Diseases; GreciaFil: Laucella, Susana Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; ArgentinaAmerican Association of Immunologists2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38456Albareda, María Cecilia; Pérez Mazliah, Damián Eduardo; Natale, Maria Ailen; Castro Eiro, Melisa Daiana; Alvarez, María G.; et al.; Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease; American Association of Immunologists; Journal of Immunology; 194; 8; 3-2015; 3883-38890022-17671550-6606CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jimmunol.org/content/194/8/3883info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.1402202info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:34Zoai:ri.conicet.gov.ar:11336/38456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:34.55CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
title Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
spellingShingle Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
Albareda, María Cecilia
T Cruzi
Chagas Disease
Il7 Receptor
title_short Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
title_full Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
title_fullStr Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
title_full_unstemmed Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
title_sort Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease
dc.creator.none.fl_str_mv Albareda, María Cecilia
Pérez Mazliah, Damián Eduardo
Natale, Maria Ailen
Castro Eiro, Melisa Daiana
Alvarez, María G.
Viotti, Rodolfo Jorge
Bertocchi, Graciela
Lococo, Bruno
Tarleton, Rick L.
Laucella, Susana Adriana
author Albareda, María Cecilia
author_facet Albareda, María Cecilia
Pérez Mazliah, Damián Eduardo
Natale, Maria Ailen
Castro Eiro, Melisa Daiana
Alvarez, María G.
Viotti, Rodolfo Jorge
Bertocchi, Graciela
Lococo, Bruno
Tarleton, Rick L.
Laucella, Susana Adriana
author_role author
author2 Pérez Mazliah, Damián Eduardo
Natale, Maria Ailen
Castro Eiro, Melisa Daiana
Alvarez, María G.
Viotti, Rodolfo Jorge
Bertocchi, Graciela
Lococo, Bruno
Tarleton, Rick L.
Laucella, Susana Adriana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv T Cruzi
Chagas Disease
Il7 Receptor
topic T Cruzi
Chagas Disease
Il7 Receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We have previously demonstrated that immune responses in subjects with chronic Trypanosoma cruzi infection display features common to other persistent infections with signs of T cell exhaustion. Alterations in cytokine receptor signal transduction have emerged as one of the cell-intrinsic mechanisms of T cell exhaustion. In this study, we performed an analysis of the expression of IL-7R components (CD127 and CD132) on CD4+ and CD8+ T cells and evaluated IL-7–dependent signaling events in patients at different clinical stages of chronic chagasic heart disease. Subjects with no signs of cardiac disease showed a decrease in CD127+CD132+ cells and a reciprocal gain of CD127−CD132+ in CD8+ and CD4+ T cells compared with either patients exhibiting heart enlargement or uninfected controls. T. cruzi infection, in vitro, was able to stimulate the downregulation of CD127 and the upregulation of CD132 on T cells. IL-7–induced phosphorylation of STAT5 as well as Bcl-2 and CD25 expression were lower in T. cruzi–infected subjects compared with uninfected controls. The serum levels of IL-7 were also increased in chronic chagasic patients. The present study highlights perturbed IL-7/IL-7R T cell signaling through STAT5 as a potential mechanism of T cell exhaustion in chronic T. cruzi infection.
Fil: Albareda, María Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Mazliah, Damián Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
Fil: Natale, Maria Ailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
Fil: Castro Eiro, Melisa Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
Fil: Alvarez, María G.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Viotti, Rodolfo Jorge. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Bertocchi, Graciela. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Lococo, Bruno. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina
Fil: Tarleton, Rick L.. Center for Tropical and Emerging Global Diseases; Grecia
Fil: Laucella, Susana Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos ; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud. Instituto Nacional de Parasitología; Argentina
description We have previously demonstrated that immune responses in subjects with chronic Trypanosoma cruzi infection display features common to other persistent infections with signs of T cell exhaustion. Alterations in cytokine receptor signal transduction have emerged as one of the cell-intrinsic mechanisms of T cell exhaustion. In this study, we performed an analysis of the expression of IL-7R components (CD127 and CD132) on CD4+ and CD8+ T cells and evaluated IL-7–dependent signaling events in patients at different clinical stages of chronic chagasic heart disease. Subjects with no signs of cardiac disease showed a decrease in CD127+CD132+ cells and a reciprocal gain of CD127−CD132+ in CD8+ and CD4+ T cells compared with either patients exhibiting heart enlargement or uninfected controls. T. cruzi infection, in vitro, was able to stimulate the downregulation of CD127 and the upregulation of CD132 on T cells. IL-7–induced phosphorylation of STAT5 as well as Bcl-2 and CD25 expression were lower in T. cruzi–infected subjects compared with uninfected controls. The serum levels of IL-7 were also increased in chronic chagasic patients. The present study highlights perturbed IL-7/IL-7R T cell signaling through STAT5 as a potential mechanism of T cell exhaustion in chronic T. cruzi infection.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38456
Albareda, María Cecilia; Pérez Mazliah, Damián Eduardo; Natale, Maria Ailen; Castro Eiro, Melisa Daiana; Alvarez, María G.; et al.; Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease; American Association of Immunologists; Journal of Immunology; 194; 8; 3-2015; 3883-3889
0022-1767
1550-6606
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38456
identifier_str_mv Albareda, María Cecilia; Pérez Mazliah, Damián Eduardo; Natale, Maria Ailen; Castro Eiro, Melisa Daiana; Alvarez, María G.; et al.; Perturbed T Cell IL-7 Receptor Signaling in Chronic Chagas Disease; American Association of Immunologists; Journal of Immunology; 194; 8; 3-2015; 3883-3889
0022-1767
1550-6606
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jimmunol.org/content/194/8/3883
info:eu-repo/semantics/altIdentifier/doi/10.4049/jimmunol.1402202
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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