Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis

Autores
Goicoechea, Hector Casimiro; Roy, Bidhan C.; Santos, Marina; Campiglia, Andres D.; Mallik, Sanku
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and c-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitativeanalysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and c-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum.
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. University of Central Florida; Estados Unidos
Fil: Roy, Bidhan C.. North Dakota State University; Estados Unidos
Fil: Santos, Marina. University of Central Florida; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Campiglia, Andres D.. University of Central Florida; Estados Unidos
Fil: Mallik, Sanku. North Dakota State University; Estados Unidos
Materia
Luminescence
Lanthanide ions
Carbonic anhydrase
gamma-Globulin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102566
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysisGoicoechea, Hector CasimiroRoy, Bidhan C.Santos, MarinaCampiglia, Andres D.Mallik, SankuLuminescenceLanthanide ionsCarbonic anhydrasegamma-Globulinhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and c-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitativeanalysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and c-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum.Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. University of Central Florida; Estados UnidosFil: Roy, Bidhan C.. North Dakota State University; Estados UnidosFil: Santos, Marina. University of Central Florida; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Campiglia, Andres D.. University of Central Florida; Estados UnidosFil: Mallik, Sanku. North Dakota State University; Estados UnidosAcademic Press Inc Elsevier Science2005-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102566Goicoechea, Hector Casimiro; Roy, Bidhan C.; Santos, Marina; Campiglia, Andres D.; Mallik, Sanku; Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis; Academic Press Inc Elsevier Science; Analytical Biochemistry; 336; 1; 1-2005; 64-740003-2697CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ab.2004.09.020info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0003269704007560info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:28Zoai:ri.conicet.gov.ar:11336/102566instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:28.964CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
title Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
spellingShingle Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
Goicoechea, Hector Casimiro
Luminescence
Lanthanide ions
Carbonic anhydrase
gamma-Globulin
title_short Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
title_full Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
title_fullStr Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
title_full_unstemmed Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
title_sort Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis
dc.creator.none.fl_str_mv Goicoechea, Hector Casimiro
Roy, Bidhan C.
Santos, Marina
Campiglia, Andres D.
Mallik, Sanku
author Goicoechea, Hector Casimiro
author_facet Goicoechea, Hector Casimiro
Roy, Bidhan C.
Santos, Marina
Campiglia, Andres D.
Mallik, Sanku
author_role author
author2 Roy, Bidhan C.
Santos, Marina
Campiglia, Andres D.
Mallik, Sanku
author2_role author
author
author
author
dc.subject.none.fl_str_mv Luminescence
Lanthanide ions
Carbonic anhydrase
gamma-Globulin
topic Luminescence
Lanthanide ions
Carbonic anhydrase
gamma-Globulin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and c-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitativeanalysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and c-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum.
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. University of Central Florida; Estados Unidos
Fil: Roy, Bidhan C.. North Dakota State University; Estados Unidos
Fil: Santos, Marina. University of Central Florida; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Campiglia, Andres D.. University of Central Florida; Estados Unidos
Fil: Mallik, Sanku. North Dakota State University; Estados Unidos
description Two lanthanide complexes, namely 5-aminosalicylic acid ethylenediaminetetraacetate europium(III) (5As-EDTA-Eu3+) and 4-aminosalicylic acid ethylenediaminetetraacetate terbium(III), were evaluated for the analysis of carbonic anhydrase, human serum albumin (HSA), and c-globulin. Quantitative analysis is based on their luminescence enhancement upon protein binding and qualitativeanalysis on their lifetime capability to recognize the binding protein. Analytical figures of merit are presented for the three proteins. The limits of detection with 5As-EDTA-Eu3+ are at the parts per billion level. Partial least square regression analysis is used to determine HSA and c-globulin in binary mixtures without previous separation at the concentration ranges typically found in clinical tests of human blood serum.
publishDate 2005
dc.date.none.fl_str_mv 2005-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102566
Goicoechea, Hector Casimiro; Roy, Bidhan C.; Santos, Marina; Campiglia, Andres D.; Mallik, Sanku; Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis; Academic Press Inc Elsevier Science; Analytical Biochemistry; 336; 1; 1-2005; 64-74
0003-2697
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102566
identifier_str_mv Goicoechea, Hector Casimiro; Roy, Bidhan C.; Santos, Marina; Campiglia, Andres D.; Mallik, Sanku; Evaluation of two lanthanide complexes for qualitative and quantitative analysis of target proteins via partial least squares analysis; Academic Press Inc Elsevier Science; Analytical Biochemistry; 336; 1; 1-2005; 64-74
0003-2697
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ab.2004.09.020
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0003269704007560
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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