Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica

Autores
Cervantes Chávez, José A.; Kronberg, Maria Florencia; Di Bernardo, Maria Susana; Ruiz Herrera, José
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies on the dimorphic transition of Yarrowia lipolytica suggested opposite roles for MAPK and PKA pathways in this phenomenon. To obtain conclusive evidences for these opposite roles we isolated and disrupted the unique gene encoding the Pka catalytic subunit (TPK1). TPK1 was regulated only at the post-transcriptional level, with Pka activity increasing during yeast-like growth. tpk1 null mutants were viable and without growth defects, but more sensitive to different stress conditions. Δtpk1 mutants were mating-deficient, and grew constitutively in the mycelial form, whereas Δste11 (Mapkkk-less)/Δtpk1 double mutants grew in the yeast form, indicating that this is the default growth pattern of the fungus. Our data confirm that MAPK and PKA pathways operate in opposition during the dimorphic behavior of Y. lipolytica, but synergic in mating. These data stress the idea that in different fungi both signal transduction systems may operate distinctly or even be antagonistic or synergic in the coordination of cell responses to different stimuli. © 2009 Elsevier Inc. All rights reserved.
Fil: Cervantes Chávez, José A.. Centro de Investigación y de Estudios Avanzados del IPN; México
Fil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invets. Bioquímicas y Fisiologicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Di Bernardo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invets. Bioquímicas y Fisiologicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Ruiz Herrera, José. Centro de Investigación y de Estudios Avanzados del IPN; México
Materia
Fungal Dimorphism
Pka Catalytic Subunit
Pka Pathway
Yarrowia Lipolytica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60709
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolyticaCervantes Chávez, José A.Kronberg, Maria FlorenciaDi Bernardo, Maria SusanaRuiz Herrera, JoséFungal DimorphismPka Catalytic SubunitPka PathwayYarrowia Lipolyticahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Previous studies on the dimorphic transition of Yarrowia lipolytica suggested opposite roles for MAPK and PKA pathways in this phenomenon. To obtain conclusive evidences for these opposite roles we isolated and disrupted the unique gene encoding the Pka catalytic subunit (TPK1). TPK1 was regulated only at the post-transcriptional level, with Pka activity increasing during yeast-like growth. tpk1 null mutants were viable and without growth defects, but more sensitive to different stress conditions. Δtpk1 mutants were mating-deficient, and grew constitutively in the mycelial form, whereas Δste11 (Mapkkk-less)/Δtpk1 double mutants grew in the yeast form, indicating that this is the default growth pattern of the fungus. Our data confirm that MAPK and PKA pathways operate in opposition during the dimorphic behavior of Y. lipolytica, but synergic in mating. These data stress the idea that in different fungi both signal transduction systems may operate distinctly or even be antagonistic or synergic in the coordination of cell responses to different stimuli. © 2009 Elsevier Inc. All rights reserved.Fil: Cervantes Chávez, José A.. Centro de Investigación y de Estudios Avanzados del IPN; MéxicoFil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invets. Bioquímicas y Fisiologicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Di Bernardo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invets. Bioquímicas y Fisiologicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Ruiz Herrera, José. Centro de Investigación y de Estudios Avanzados del IPN; MéxicoAcademic Press Inc Elsevier Science2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60709Cervantes Chávez, José A.; Kronberg, Maria Florencia; Di Bernardo, Maria Susana; Ruiz Herrera, José; Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 46; 5; 5-2009; 390-3991087-1845CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fgb.2009.02.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1087184509000383info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:58Zoai:ri.conicet.gov.ar:11336/60709instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:59.018CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
title Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
spellingShingle Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
Cervantes Chávez, José A.
Fungal Dimorphism
Pka Catalytic Subunit
Pka Pathway
Yarrowia Lipolytica
title_short Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
title_full Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
title_fullStr Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
title_full_unstemmed Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
title_sort Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica
dc.creator.none.fl_str_mv Cervantes Chávez, José A.
Kronberg, Maria Florencia
Di Bernardo, Maria Susana
Ruiz Herrera, José
author Cervantes Chávez, José A.
author_facet Cervantes Chávez, José A.
Kronberg, Maria Florencia
Di Bernardo, Maria Susana
Ruiz Herrera, José
author_role author
author2 Kronberg, Maria Florencia
Di Bernardo, Maria Susana
Ruiz Herrera, José
author2_role author
author
author
dc.subject.none.fl_str_mv Fungal Dimorphism
Pka Catalytic Subunit
Pka Pathway
Yarrowia Lipolytica
topic Fungal Dimorphism
Pka Catalytic Subunit
Pka Pathway
Yarrowia Lipolytica
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Previous studies on the dimorphic transition of Yarrowia lipolytica suggested opposite roles for MAPK and PKA pathways in this phenomenon. To obtain conclusive evidences for these opposite roles we isolated and disrupted the unique gene encoding the Pka catalytic subunit (TPK1). TPK1 was regulated only at the post-transcriptional level, with Pka activity increasing during yeast-like growth. tpk1 null mutants were viable and without growth defects, but more sensitive to different stress conditions. Δtpk1 mutants were mating-deficient, and grew constitutively in the mycelial form, whereas Δste11 (Mapkkk-less)/Δtpk1 double mutants grew in the yeast form, indicating that this is the default growth pattern of the fungus. Our data confirm that MAPK and PKA pathways operate in opposition during the dimorphic behavior of Y. lipolytica, but synergic in mating. These data stress the idea that in different fungi both signal transduction systems may operate distinctly or even be antagonistic or synergic in the coordination of cell responses to different stimuli. © 2009 Elsevier Inc. All rights reserved.
Fil: Cervantes Chávez, José A.. Centro de Investigación y de Estudios Avanzados del IPN; México
Fil: Kronberg, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invets. Bioquímicas y Fisiologicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Di Bernardo, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invets. Bioquímicas y Fisiologicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Ruiz Herrera, José. Centro de Investigación y de Estudios Avanzados del IPN; México
description Previous studies on the dimorphic transition of Yarrowia lipolytica suggested opposite roles for MAPK and PKA pathways in this phenomenon. To obtain conclusive evidences for these opposite roles we isolated and disrupted the unique gene encoding the Pka catalytic subunit (TPK1). TPK1 was regulated only at the post-transcriptional level, with Pka activity increasing during yeast-like growth. tpk1 null mutants were viable and without growth defects, but more sensitive to different stress conditions. Δtpk1 mutants were mating-deficient, and grew constitutively in the mycelial form, whereas Δste11 (Mapkkk-less)/Δtpk1 double mutants grew in the yeast form, indicating that this is the default growth pattern of the fungus. Our data confirm that MAPK and PKA pathways operate in opposition during the dimorphic behavior of Y. lipolytica, but synergic in mating. These data stress the idea that in different fungi both signal transduction systems may operate distinctly or even be antagonistic or synergic in the coordination of cell responses to different stimuli. © 2009 Elsevier Inc. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60709
Cervantes Chávez, José A.; Kronberg, Maria Florencia; Di Bernardo, Maria Susana; Ruiz Herrera, José; Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 46; 5; 5-2009; 390-399
1087-1845
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60709
identifier_str_mv Cervantes Chávez, José A.; Kronberg, Maria Florencia; Di Bernardo, Maria Susana; Ruiz Herrera, José; Regulatory role of the PKA pathway in dimorphism and mating in Yarrowia lipolytica; Academic Press Inc Elsevier Science; Fungal Genetics And Biology; 46; 5; 5-2009; 390-399
1087-1845
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fgb.2009.02.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1087184509000383
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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