Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)

Autores
López, Agustín Ignacio; Moschen, Sebastián Nicolás; Villan, Claudio Sebastian; Lopez Fernandez, Maria Paula; Maldonado, Sara Beatriz; Paniego, Norma Beatriz; Heinz, Ruth Amelia; Fernández, Paula del Carmen
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables.
Fil: López, Agustín Ignacio. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moschen, Sebastián Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villan, Claudio Sebastian. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Lopez Fernandez, Maria Paula. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heinz, Ruth Amelia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Paula del Carmen. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
HELIANTHUS ANNUUS
LEAF SENESCENCE
FLOW CYTOMETRY
PHYSIOLOGICAL ANALYSYS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46642
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/46642
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)López, Agustín IgnacioMoschen, Sebastián NicolásVillan, Claudio SebastianLopez Fernandez, Maria PaulaMaldonado, Sara BeatrizPaniego, Norma BeatrizHeinz, Ruth AmeliaFernández, Paula del CarmenHELIANTHUS ANNUUSLEAF SENESCENCEFLOW CYTOMETRYPHYSIOLOGICAL ANALYSYShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables.Fil: López, Agustín Ignacio. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moschen, Sebastián Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villan, Claudio Sebastian. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Lopez Fernandez, Maria Paula. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Heinz, Ruth Amelia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández, Paula del Carmen. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46642López, Agustín Ignacio; Moschen, Sebastián Nicolás; Villan, Claudio Sebastian; Lopez Fernandez, Maria Paula; Maldonado, Sara Beatriz; et al.; Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.); Elsevier Ireland; Plant Science; 250; 9-2016; 40-500168-94521873-2259CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168945216300966info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2016.05.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:16Zoai:ri.conicet.gov.ar:11336/46642instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:16.409CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
title Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
spellingShingle Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
López, Agustín Ignacio
HELIANTHUS ANNUUS
LEAF SENESCENCE
FLOW CYTOMETRY
PHYSIOLOGICAL ANALYSYS
title_short Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
title_full Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
title_fullStr Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
title_full_unstemmed Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
title_sort Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.)
dc.creator.none.fl_str_mv López, Agustín Ignacio
Moschen, Sebastián Nicolás
Villan, Claudio Sebastian
Lopez Fernandez, Maria Paula
Maldonado, Sara Beatriz
Paniego, Norma Beatriz
Heinz, Ruth Amelia
Fernández, Paula del Carmen
author López, Agustín Ignacio
author_facet López, Agustín Ignacio
Moschen, Sebastián Nicolás
Villan, Claudio Sebastian
Lopez Fernandez, Maria Paula
Maldonado, Sara Beatriz
Paniego, Norma Beatriz
Heinz, Ruth Amelia
Fernández, Paula del Carmen
author_role author
author2 Moschen, Sebastián Nicolás
Villan, Claudio Sebastian
Lopez Fernandez, Maria Paula
Maldonado, Sara Beatriz
Paniego, Norma Beatriz
Heinz, Ruth Amelia
Fernández, Paula del Carmen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HELIANTHUS ANNUUS
LEAF SENESCENCE
FLOW CYTOMETRY
PHYSIOLOGICAL ANALYSYS
topic HELIANTHUS ANNUUS
LEAF SENESCENCE
FLOW CYTOMETRY
PHYSIOLOGICAL ANALYSYS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables.
Fil: López, Agustín Ignacio. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moschen, Sebastián Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villan, Claudio Sebastian. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Lopez Fernandez, Maria Paula. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heinz, Ruth Amelia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Paula del Carmen. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Leaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46642
López, Agustín Ignacio; Moschen, Sebastián Nicolás; Villan, Claudio Sebastian; Lopez Fernandez, Maria Paula; Maldonado, Sara Beatriz; et al.; Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.); Elsevier Ireland; Plant Science; 250; 9-2016; 40-50
0168-9452
1873-2259
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46642
identifier_str_mv López, Agustín Ignacio; Moschen, Sebastián Nicolás; Villan, Claudio Sebastian; Lopez Fernandez, Maria Paula; Maldonado, Sara Beatriz; et al.; Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies ( Helianthus annuus L.); Elsevier Ireland; Plant Science; 250; 9-2016; 40-50
0168-9452
1873-2259
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168945216300966
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2016.05.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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