Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?

Autores
Gamboa León, Rubi; Gonzalez Ramirez, Claudia; Padilla Raygoza, Nicolas; Sosa-Estani, Sergio Alejandro; Caamal Kantun, Alejandra; Buekens, Pierre; Dumonteil, Eric
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8-0.9% of prevalence for T. cruzi-specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.
Fil: Gamboa León, Rubi. University of Tulane; Estados Unidos
Fil: Gonzalez Ramirez, Claudia. Universidad Autónoma de Yucatán; México
Fil: Padilla Raygoza, Nicolas. Universidad Autónoma de Yucatán; México
Fil: Sosa-Estani, Sergio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina
Fil: Caamal Kantun, Alejandra. Universidad Autónoma de Yucatán; México
Fil: Buekens, Pierre. University of Tulane; Estados Unidos
Fil: Dumonteil, Eric. University of Tulane; Estados Unidos
Materia
TRUPANOSOMA CRUZI
SEROLOGY
CHAGAS DISEASE
MEXICO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192407
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192407
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?Gamboa León, RubiGonzalez Ramirez, ClaudiaPadilla Raygoza, NicolasSosa-Estani, Sergio AlejandroCaamal Kantun, AlejandraBuekens, PierreDumonteil, EricTRUPANOSOMA CRUZISEROLOGYCHAGAS DISEASEMEXICOhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8-0.9% of prevalence for T. cruzi-specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.Fil: Gamboa León, Rubi. University of Tulane; Estados UnidosFil: Gonzalez Ramirez, Claudia. Universidad Autónoma de Yucatán; MéxicoFil: Padilla Raygoza, Nicolas. Universidad Autónoma de Yucatán; MéxicoFil: Sosa-Estani, Sergio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; ArgentinaFil: Caamal Kantun, Alejandra. Universidad Autónoma de Yucatán; MéxicoFil: Buekens, Pierre. University of Tulane; Estados UnidosFil: Dumonteil, Eric. University of Tulane; Estados UnidosAmerican Society of Parasitologists2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192407Gamboa León, Rubi; Gonzalez Ramirez, Claudia; Padilla Raygoza, Nicolas; Sosa-Estani, Sergio Alejandro; Caamal Kantun, Alejandra; et al.; Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?; American Society of Parasitologists; Journal of Parasitology; 97; 2; 4-2011; 338-3430022-3395CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/full/10.1645/GE-2545.1info:eu-repo/semantics/altIdentifier/doi/10.1645/GE-2545.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:10:47Zoai:ri.conicet.gov.ar:11336/192407instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:10:47.331CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
title Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
spellingShingle Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
Gamboa León, Rubi
TRUPANOSOMA CRUZI
SEROLOGY
CHAGAS DISEASE
MEXICO
title_short Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
title_full Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
title_fullStr Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
title_full_unstemmed Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
title_sort Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?
dc.creator.none.fl_str_mv Gamboa León, Rubi
Gonzalez Ramirez, Claudia
Padilla Raygoza, Nicolas
Sosa-Estani, Sergio Alejandro
Caamal Kantun, Alejandra
Buekens, Pierre
Dumonteil, Eric
author Gamboa León, Rubi
author_facet Gamboa León, Rubi
Gonzalez Ramirez, Claudia
Padilla Raygoza, Nicolas
Sosa-Estani, Sergio Alejandro
Caamal Kantun, Alejandra
Buekens, Pierre
Dumonteil, Eric
author_role author
author2 Gonzalez Ramirez, Claudia
Padilla Raygoza, Nicolas
Sosa-Estani, Sergio Alejandro
Caamal Kantun, Alejandra
Buekens, Pierre
Dumonteil, Eric
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv TRUPANOSOMA CRUZI
SEROLOGY
CHAGAS DISEASE
MEXICO
topic TRUPANOSOMA CRUZI
SEROLOGY
CHAGAS DISEASE
MEXICO
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8-0.9% of prevalence for T. cruzi-specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.
Fil: Gamboa León, Rubi. University of Tulane; Estados Unidos
Fil: Gonzalez Ramirez, Claudia. Universidad Autónoma de Yucatán; México
Fil: Padilla Raygoza, Nicolas. Universidad Autónoma de Yucatán; México
Fil: Sosa-Estani, Sergio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina
Fil: Caamal Kantun, Alejandra. Universidad Autónoma de Yucatán; México
Fil: Buekens, Pierre. University of Tulane; Estados Unidos
Fil: Dumonteil, Eric. University of Tulane; Estados Unidos
description We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8-0.9% of prevalence for T. cruzi-specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192407
Gamboa León, Rubi; Gonzalez Ramirez, Claudia; Padilla Raygoza, Nicolas; Sosa-Estani, Sergio Alejandro; Caamal Kantun, Alejandra; et al.; Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?; American Society of Parasitologists; Journal of Parasitology; 97; 2; 4-2011; 338-343
0022-3395
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192407
identifier_str_mv Gamboa León, Rubi; Gonzalez Ramirez, Claudia; Padilla Raygoza, Nicolas; Sosa-Estani, Sergio Alejandro; Caamal Kantun, Alejandra; et al.; Do Commercial Serologic Tests for Trypanosoma cruzi Infection Detect Mexican Strains in Women and Newborns?; American Society of Parasitologists; Journal of Parasitology; 97; 2; 4-2011; 338-343
0022-3395
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/full/10.1645/GE-2545.1
info:eu-repo/semantics/altIdentifier/doi/10.1645/GE-2545.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Parasitologists
publisher.none.fl_str_mv American Society of Parasitologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782495525175296
score 12.982451

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