Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells

Autores
Tate, Pablo Sebastián; Marazita, Mariela Claudia; Marquioni Ramella, Melisa Daniela; Suburo, Angela Maria
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aged-related macular degeneration (AMD), a prevalent chronic disease leading to blindness, is associated with oxidative damage of the retinal pigment epithelium (RPE). Ilex paraguariensis (yerba mate, YM), widely consumed in South America, is an excellent source of antioxidants and could prevent RPE cell impairment. Therefore, we evaluated the effects of YM extracts or caffeic (CAF) and chlorogenic (CHL) acids, two major Ilex polyphenols on cell death or premature senescence in a cell line derived from human RPE (ARPE-19). Cultures were incubated with YM, CAF, CHL or appropriate controls and then exposed to H2O2. Protection was correlated with decreases of reactive oxygen species (ROS) and DNA breaks, phosphorylation of the cAMP response element (CREB) and increases of the nuclear factor erythroid 2-related factor 2 (NRF2), BCL2, superoxide dismutase (SOD2) and sirtuin 1 (SIRT1). These results support the protective role of YM and its polyphenols against oxidative stress induced RPE injury.
Fil: Tate, Pablo Sebastián. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Fil: Marazita, Mariela Claudia. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Fil: Marquioni Ramella, Melisa Daniela. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Fil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Materia
YERBA MATE
SENESCENCIA
DMAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163867
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cellsTate, Pablo SebastiánMarazita, Mariela ClaudiaMarquioni Ramella, Melisa DanielaSuburo, Angela MariaYERBA MATESENESCENCIADMAEhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Aged-related macular degeneration (AMD), a prevalent chronic disease leading to blindness, is associated with oxidative damage of the retinal pigment epithelium (RPE). Ilex paraguariensis (yerba mate, YM), widely consumed in South America, is an excellent source of antioxidants and could prevent RPE cell impairment. Therefore, we evaluated the effects of YM extracts or caffeic (CAF) and chlorogenic (CHL) acids, two major Ilex polyphenols on cell death or premature senescence in a cell line derived from human RPE (ARPE-19). Cultures were incubated with YM, CAF, CHL or appropriate controls and then exposed to H2O2. Protection was correlated with decreases of reactive oxygen species (ROS) and DNA breaks, phosphorylation of the cAMP response element (CREB) and increases of the nuclear factor erythroid 2-related factor 2 (NRF2), BCL2, superoxide dismutase (SOD2) and sirtuin 1 (SIRT1). These results support the protective role of YM and its polyphenols against oxidative stress induced RPE injury.Fil: Tate, Pablo Sebastián. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; ArgentinaFil: Marazita, Mariela Claudia. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; ArgentinaFil: Marquioni Ramella, Melisa Daniela. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; ArgentinaFil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; ArgentinaElsevier2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163867Tate, Pablo Sebastián; Marazita, Mariela Claudia; Marquioni Ramella, Melisa Daniela; Suburo, Angela Maria; Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells; Elsevier; Journal of Functional Foods; 67; 4-2020; 107-8541756-4646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1756464620300578info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2020.103833info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:18Zoai:ri.conicet.gov.ar:11336/163867instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:19.102CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
title Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
spellingShingle Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
Tate, Pablo Sebastián
YERBA MATE
SENESCENCIA
DMAE
title_short Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
title_full Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
title_fullStr Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
title_full_unstemmed Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
title_sort Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells
dc.creator.none.fl_str_mv Tate, Pablo Sebastián
Marazita, Mariela Claudia
Marquioni Ramella, Melisa Daniela
Suburo, Angela Maria
author Tate, Pablo Sebastián
author_facet Tate, Pablo Sebastián
Marazita, Mariela Claudia
Marquioni Ramella, Melisa Daniela
Suburo, Angela Maria
author_role author
author2 Marazita, Mariela Claudia
Marquioni Ramella, Melisa Daniela
Suburo, Angela Maria
author2_role author
author
author
dc.subject.none.fl_str_mv YERBA MATE
SENESCENCIA
DMAE
topic YERBA MATE
SENESCENCIA
DMAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aged-related macular degeneration (AMD), a prevalent chronic disease leading to blindness, is associated with oxidative damage of the retinal pigment epithelium (RPE). Ilex paraguariensis (yerba mate, YM), widely consumed in South America, is an excellent source of antioxidants and could prevent RPE cell impairment. Therefore, we evaluated the effects of YM extracts or caffeic (CAF) and chlorogenic (CHL) acids, two major Ilex polyphenols on cell death or premature senescence in a cell line derived from human RPE (ARPE-19). Cultures were incubated with YM, CAF, CHL or appropriate controls and then exposed to H2O2. Protection was correlated with decreases of reactive oxygen species (ROS) and DNA breaks, phosphorylation of the cAMP response element (CREB) and increases of the nuclear factor erythroid 2-related factor 2 (NRF2), BCL2, superoxide dismutase (SOD2) and sirtuin 1 (SIRT1). These results support the protective role of YM and its polyphenols against oxidative stress induced RPE injury.
Fil: Tate, Pablo Sebastián. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Fil: Marazita, Mariela Claudia. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Fil: Marquioni Ramella, Melisa Daniela. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
Fil: Suburo, Angela Maria. Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Medicina Traslacional; Argentina
description Aged-related macular degeneration (AMD), a prevalent chronic disease leading to blindness, is associated with oxidative damage of the retinal pigment epithelium (RPE). Ilex paraguariensis (yerba mate, YM), widely consumed in South America, is an excellent source of antioxidants and could prevent RPE cell impairment. Therefore, we evaluated the effects of YM extracts or caffeic (CAF) and chlorogenic (CHL) acids, two major Ilex polyphenols on cell death or premature senescence in a cell line derived from human RPE (ARPE-19). Cultures were incubated with YM, CAF, CHL or appropriate controls and then exposed to H2O2. Protection was correlated with decreases of reactive oxygen species (ROS) and DNA breaks, phosphorylation of the cAMP response element (CREB) and increases of the nuclear factor erythroid 2-related factor 2 (NRF2), BCL2, superoxide dismutase (SOD2) and sirtuin 1 (SIRT1). These results support the protective role of YM and its polyphenols against oxidative stress induced RPE injury.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163867
Tate, Pablo Sebastián; Marazita, Mariela Claudia; Marquioni Ramella, Melisa Daniela; Suburo, Angela Maria; Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells; Elsevier; Journal of Functional Foods; 67; 4-2020; 107-854
1756-4646
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163867
identifier_str_mv Tate, Pablo Sebastián; Marazita, Mariela Claudia; Marquioni Ramella, Melisa Daniela; Suburo, Angela Maria; Ilex paraguariensis extracts and its polyphenols prevent oxidative damage and senescence of human retinal pigment epithelium cells; Elsevier; Journal of Functional Foods; 67; 4-2020; 107-854
1756-4646
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1756464620300578
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2020.103833
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.238319

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