The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis

Autores
Bini, Estela Isabel; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Barrios Payán, Jorge; Colucci, Darío; Cruz, Alejandro Francisco; Zatarain, Zyanya Lucía; Alfonseca, Edgar; Pardo, Marta Romano; Bottasso, Oscar Adelmo; Hernández Pando, Rogelio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The relation between men and women suffering pulmonary tuberculosis is 7/3 in favor to males. Sex hormones could be a significant factor for this difference, considering that testosterone impairs macrophage activation and pro-inflammatory cytokines production, while estrogens are proinflammatory mediator's inducer. The aim of this work was to compare the evolution of tuberculosis in male and female mice using a model of progressive disease. BALB/c mice, male and female were randomized into two groups: castrated or sham-operated, and infected by the intratracheal route with a high dose of Mycobacterium tuberculosis strain H37Rv. Mice were euthanized at different time points and in their lungs were determined bacilli loads, inflammation, cytokines expression, survival and testosterone levels in serum. Non-castrated male mice showed significant higher mortality and bacilli burdens during late disease than female and castrated male animals. Compared to males, females and castrated males exhibited significant higher inflammation in all lung compartments, earlier formation of granulomas and pneumonia, while between castrated and non-castrated females there were not significant differences. Females and castrated males expressed significant higher TNF-α, IFN γ, IL12, iNOS and IL17 than non-castrated males during the first month of infection. Serum Testosterone of males showed higher concentration during late infection. Orchidectomy at day 60 post-infection produced a significant decrease of bacilli burdens in coexistence with higher expression of TNFα, IL-12 and IFNγ. Thus, male mice are more susceptible to tuberculosis than females and this was prevented by castration suggesting that testosterone could be a tuberculosis susceptibility factor.
Fil: Bini, Estela Isabel. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Mata Espinosa, Dulce. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Marquina Castillo, Brenda. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Barrios Payán, Jorge. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Colucci, Darío. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Cruz, Alejandro Francisco. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Zatarain, Zyanya Lucía. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Alfonseca, Edgar. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Pardo, Marta Romano. Centro de Investigación y de Estudios Avanzados; México
Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; Argentina
Fil: Hernández Pando, Rogelio. Instituto Nacional de la Nutrición Salvador Zubiran; México
Materia
TUBERCULOSIS
SEX HORMONES
GENDER
EXPERIMENTAL PATHOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94403
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94403
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosisBini, Estela IsabelMata Espinosa, DulceMarquina Castillo, BrendaBarrios Payán, JorgeColucci, DaríoCruz, Alejandro FranciscoZatarain, Zyanya LucíaAlfonseca, EdgarPardo, Marta RomanoBottasso, Oscar AdelmoHernández Pando, RogelioTUBERCULOSISSEX HORMONESGENDEREXPERIMENTAL PATHOLOGYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The relation between men and women suffering pulmonary tuberculosis is 7/3 in favor to males. Sex hormones could be a significant factor for this difference, considering that testosterone impairs macrophage activation and pro-inflammatory cytokines production, while estrogens are proinflammatory mediator's inducer. The aim of this work was to compare the evolution of tuberculosis in male and female mice using a model of progressive disease. BALB/c mice, male and female were randomized into two groups: castrated or sham-operated, and infected by the intratracheal route with a high dose of Mycobacterium tuberculosis strain H37Rv. Mice were euthanized at different time points and in their lungs were determined bacilli loads, inflammation, cytokines expression, survival and testosterone levels in serum. Non-castrated male mice showed significant higher mortality and bacilli burdens during late disease than female and castrated male animals. Compared to males, females and castrated males exhibited significant higher inflammation in all lung compartments, earlier formation of granulomas and pneumonia, while between castrated and non-castrated females there were not significant differences. Females and castrated males expressed significant higher TNF-α, IFN γ, IL12, iNOS and IL17 than non-castrated males during the first month of infection. Serum Testosterone of males showed higher concentration during late infection. Orchidectomy at day 60 post-infection produced a significant decrease of bacilli burdens in coexistence with higher expression of TNFα, IL-12 and IFNγ. Thus, male mice are more susceptible to tuberculosis than females and this was prevented by castration suggesting that testosterone could be a tuberculosis susceptibility factor.Fil: Bini, Estela Isabel. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoFil: Mata Espinosa, Dulce. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoFil: Marquina Castillo, Brenda. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoFil: Barrios Payán, Jorge. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoFil: Colucci, Darío. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; ArgentinaFil: Cruz, Alejandro Francisco. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoFil: Zatarain, Zyanya Lucía. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoFil: Alfonseca, Edgar. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoFil: Pardo, Marta Romano. Centro de Investigación y de Estudios Avanzados; MéxicoFil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; ArgentinaFil: Hernández Pando, Rogelio. Instituto Nacional de la Nutrición Salvador Zubiran; MéxicoPublic Library of Science2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94403Bini, Estela Isabel; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Barrios Payán, Jorge; Colucci, Darío; et al.; The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis; Public Library of Science; Plos One; 9; 4; 4-2014; 1-81932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0093831info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0093831info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:56:06Zoai:ri.conicet.gov.ar:11336/94403instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:56:07.181CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
title The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
spellingShingle The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
Bini, Estela Isabel
TUBERCULOSIS
SEX HORMONES
GENDER
EXPERIMENTAL PATHOLOGY
title_short The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
title_full The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
title_fullStr The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
title_full_unstemmed The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
title_sort The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis
dc.creator.none.fl_str_mv Bini, Estela Isabel
Mata Espinosa, Dulce
Marquina Castillo, Brenda
Barrios Payán, Jorge
Colucci, Darío
Cruz, Alejandro Francisco
Zatarain, Zyanya Lucía
Alfonseca, Edgar
Pardo, Marta Romano
Bottasso, Oscar Adelmo
Hernández Pando, Rogelio
author Bini, Estela Isabel
author_facet Bini, Estela Isabel
Mata Espinosa, Dulce
Marquina Castillo, Brenda
Barrios Payán, Jorge
Colucci, Darío
Cruz, Alejandro Francisco
Zatarain, Zyanya Lucía
Alfonseca, Edgar
Pardo, Marta Romano
Bottasso, Oscar Adelmo
Hernández Pando, Rogelio
author_role author
author2 Mata Espinosa, Dulce
Marquina Castillo, Brenda
Barrios Payán, Jorge
Colucci, Darío
Cruz, Alejandro Francisco
Zatarain, Zyanya Lucía
Alfonseca, Edgar
Pardo, Marta Romano
Bottasso, Oscar Adelmo
Hernández Pando, Rogelio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TUBERCULOSIS
SEX HORMONES
GENDER
EXPERIMENTAL PATHOLOGY
topic TUBERCULOSIS
SEX HORMONES
GENDER
EXPERIMENTAL PATHOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The relation between men and women suffering pulmonary tuberculosis is 7/3 in favor to males. Sex hormones could be a significant factor for this difference, considering that testosterone impairs macrophage activation and pro-inflammatory cytokines production, while estrogens are proinflammatory mediator's inducer. The aim of this work was to compare the evolution of tuberculosis in male and female mice using a model of progressive disease. BALB/c mice, male and female were randomized into two groups: castrated or sham-operated, and infected by the intratracheal route with a high dose of Mycobacterium tuberculosis strain H37Rv. Mice were euthanized at different time points and in their lungs were determined bacilli loads, inflammation, cytokines expression, survival and testosterone levels in serum. Non-castrated male mice showed significant higher mortality and bacilli burdens during late disease than female and castrated male animals. Compared to males, females and castrated males exhibited significant higher inflammation in all lung compartments, earlier formation of granulomas and pneumonia, while between castrated and non-castrated females there were not significant differences. Females and castrated males expressed significant higher TNF-α, IFN γ, IL12, iNOS and IL17 than non-castrated males during the first month of infection. Serum Testosterone of males showed higher concentration during late infection. Orchidectomy at day 60 post-infection produced a significant decrease of bacilli burdens in coexistence with higher expression of TNFα, IL-12 and IFNγ. Thus, male mice are more susceptible to tuberculosis than females and this was prevented by castration suggesting that testosterone could be a tuberculosis susceptibility factor.
Fil: Bini, Estela Isabel. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Mata Espinosa, Dulce. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Marquina Castillo, Brenda. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Barrios Payán, Jorge. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Colucci, Darío. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Cruz, Alejandro Francisco. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Zatarain, Zyanya Lucía. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Alfonseca, Edgar. Instituto Nacional de la Nutrición Salvador Zubiran; México
Fil: Pardo, Marta Romano. Centro de Investigación y de Estudios Avanzados; México
Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; Argentina
Fil: Hernández Pando, Rogelio. Instituto Nacional de la Nutrición Salvador Zubiran; México
description The relation between men and women suffering pulmonary tuberculosis is 7/3 in favor to males. Sex hormones could be a significant factor for this difference, considering that testosterone impairs macrophage activation and pro-inflammatory cytokines production, while estrogens are proinflammatory mediator's inducer. The aim of this work was to compare the evolution of tuberculosis in male and female mice using a model of progressive disease. BALB/c mice, male and female were randomized into two groups: castrated or sham-operated, and infected by the intratracheal route with a high dose of Mycobacterium tuberculosis strain H37Rv. Mice were euthanized at different time points and in their lungs were determined bacilli loads, inflammation, cytokines expression, survival and testosterone levels in serum. Non-castrated male mice showed significant higher mortality and bacilli burdens during late disease than female and castrated male animals. Compared to males, females and castrated males exhibited significant higher inflammation in all lung compartments, earlier formation of granulomas and pneumonia, while between castrated and non-castrated females there were not significant differences. Females and castrated males expressed significant higher TNF-α, IFN γ, IL12, iNOS and IL17 than non-castrated males during the first month of infection. Serum Testosterone of males showed higher concentration during late infection. Orchidectomy at day 60 post-infection produced a significant decrease of bacilli burdens in coexistence with higher expression of TNFα, IL-12 and IFNγ. Thus, male mice are more susceptible to tuberculosis than females and this was prevented by castration suggesting that testosterone could be a tuberculosis susceptibility factor.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94403
Bini, Estela Isabel; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Barrios Payán, Jorge; Colucci, Darío; et al.; The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis; Public Library of Science; Plos One; 9; 4; 4-2014; 1-8
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94403
identifier_str_mv Bini, Estela Isabel; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Barrios Payán, Jorge; Colucci, Darío; et al.; The influence of sex steroid hormones in the immunopathology of experimental pulmonary tuberculosis; Public Library of Science; Plos One; 9; 4; 4-2014; 1-8
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0093831
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0093831
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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