The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis

Autores
Hernández Pando, Rogelio; Barrios Payán, Jorge; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Hernández Ramírez, Diego; Bottasso, Oscar Adelmo; Bini, Estela Isabel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The high mobility group box 1 (HMGB1) is the prototype of alarmin protein released by stressed or dying cells. The redox state of this protein confers different functions in the regulation of inflammation and immune response. Aim: Determine the kinetics, cellular sources and function of HMGB1 in experimental tuberculosis. Methods: BALB/c mice were infected with Mycobacterium tuberculosis strain H37Rv. At different time points, HMGB1 was quantified in bronchial lavage fluid (BALF) and in lungs was determined its cellular sources by immunohistochemistry. HMGB1 was blocked with specific antibodies or recombinant HMGB1 was administered during early or late infection. Bacilli burdens, inflammation and cytokines expression were determined. Results: The maximal concentration of HMGB1 in BALF was at day one of infection. Bronchial epithelium and macrophages were the most important sources. At day 7 to 21 the oxidized HMGB1 was predominant, while during late infection only the reduced form was seen. Blocking HMGB1 during early infection produced significant decrease of bacilli burdens and high production of pro-inflammatory cytokines, while the opposite was seen when HMGB1 was administered. Blocking HMGB1 activity or administrated it in high amounts during late infection worsening the disease.
Fil: Hernández Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Barrios Payán, Jorge. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Mata Espinosa, Dulce. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Marquina Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Hernández Ramírez, Diego. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; Argentina
Fil: Bini, Estela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; Argentina. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Materia
HMGB1
TUBERCULOSIS
ALARMINS
EXPERIMENTAL MODELS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52536
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52536
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosisHernández Pando, RogelioBarrios Payán, JorgeMata Espinosa, DulceMarquina Castillo, BrendaHernández Ramírez, DiegoBottasso, Oscar AdelmoBini, Estela IsabelHMGB1TUBERCULOSISALARMINSEXPERIMENTAL MODELShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: The high mobility group box 1 (HMGB1) is the prototype of alarmin protein released by stressed or dying cells. The redox state of this protein confers different functions in the regulation of inflammation and immune response. Aim: Determine the kinetics, cellular sources and function of HMGB1 in experimental tuberculosis. Methods: BALB/c mice were infected with Mycobacterium tuberculosis strain H37Rv. At different time points, HMGB1 was quantified in bronchial lavage fluid (BALF) and in lungs was determined its cellular sources by immunohistochemistry. HMGB1 was blocked with specific antibodies or recombinant HMGB1 was administered during early or late infection. Bacilli burdens, inflammation and cytokines expression were determined. Results: The maximal concentration of HMGB1 in BALF was at day one of infection. Bronchial epithelium and macrophages were the most important sources. At day 7 to 21 the oxidized HMGB1 was predominant, while during late infection only the reduced form was seen. Blocking HMGB1 during early infection produced significant decrease of bacilli burdens and high production of pro-inflammatory cytokines, while the opposite was seen when HMGB1 was administered. Blocking HMGB1 activity or administrated it in high amounts during late infection worsening the disease.Fil: Hernández Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoFil: Barrios Payán, Jorge. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoFil: Mata Espinosa, Dulce. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoFil: Marquina Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoFil: Hernández Ramírez, Diego. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoFil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; ArgentinaFil: Bini, Estela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; Argentina. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; MéxicoPublic Library of Science2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52536Hernández Pando, Rogelio; Barrios Payán, Jorge; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Hernández Ramírez, Diego; et al.; The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis; Public Library of Science; Plos One; 10; 7; 7-2015; 1-14; e01332001932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0133200info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:39:40Zoai:ri.conicet.gov.ar:11336/52536instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:39:40.713CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
title The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
spellingShingle The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
Hernández Pando, Rogelio
HMGB1
TUBERCULOSIS
ALARMINS
EXPERIMENTAL MODELS
title_short The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
title_full The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
title_fullStr The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
title_full_unstemmed The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
title_sort The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis
dc.creator.none.fl_str_mv Hernández Pando, Rogelio
Barrios Payán, Jorge
Mata Espinosa, Dulce
Marquina Castillo, Brenda
Hernández Ramírez, Diego
Bottasso, Oscar Adelmo
Bini, Estela Isabel
author Hernández Pando, Rogelio
author_facet Hernández Pando, Rogelio
Barrios Payán, Jorge
Mata Espinosa, Dulce
Marquina Castillo, Brenda
Hernández Ramírez, Diego
Bottasso, Oscar Adelmo
Bini, Estela Isabel
author_role author
author2 Barrios Payán, Jorge
Mata Espinosa, Dulce
Marquina Castillo, Brenda
Hernández Ramírez, Diego
Bottasso, Oscar Adelmo
Bini, Estela Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv HMGB1
TUBERCULOSIS
ALARMINS
EXPERIMENTAL MODELS
topic HMGB1
TUBERCULOSIS
ALARMINS
EXPERIMENTAL MODELS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: The high mobility group box 1 (HMGB1) is the prototype of alarmin protein released by stressed or dying cells. The redox state of this protein confers different functions in the regulation of inflammation and immune response. Aim: Determine the kinetics, cellular sources and function of HMGB1 in experimental tuberculosis. Methods: BALB/c mice were infected with Mycobacterium tuberculosis strain H37Rv. At different time points, HMGB1 was quantified in bronchial lavage fluid (BALF) and in lungs was determined its cellular sources by immunohistochemistry. HMGB1 was blocked with specific antibodies or recombinant HMGB1 was administered during early or late infection. Bacilli burdens, inflammation and cytokines expression were determined. Results: The maximal concentration of HMGB1 in BALF was at day one of infection. Bronchial epithelium and macrophages were the most important sources. At day 7 to 21 the oxidized HMGB1 was predominant, while during late infection only the reduced form was seen. Blocking HMGB1 during early infection produced significant decrease of bacilli burdens and high production of pro-inflammatory cytokines, while the opposite was seen when HMGB1 was administered. Blocking HMGB1 activity or administrated it in high amounts during late infection worsening the disease.
Fil: Hernández Pando, Rogelio. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Barrios Payán, Jorge. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Mata Espinosa, Dulce. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Marquina Castillo, Brenda. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Hernández Ramírez, Diego. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; Argentina
Fil: Bini, Estela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; Argentina. Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán"; México
description Background: The high mobility group box 1 (HMGB1) is the prototype of alarmin protein released by stressed or dying cells. The redox state of this protein confers different functions in the regulation of inflammation and immune response. Aim: Determine the kinetics, cellular sources and function of HMGB1 in experimental tuberculosis. Methods: BALB/c mice were infected with Mycobacterium tuberculosis strain H37Rv. At different time points, HMGB1 was quantified in bronchial lavage fluid (BALF) and in lungs was determined its cellular sources by immunohistochemistry. HMGB1 was blocked with specific antibodies or recombinant HMGB1 was administered during early or late infection. Bacilli burdens, inflammation and cytokines expression were determined. Results: The maximal concentration of HMGB1 in BALF was at day one of infection. Bronchial epithelium and macrophages were the most important sources. At day 7 to 21 the oxidized HMGB1 was predominant, while during late infection only the reduced form was seen. Blocking HMGB1 during early infection produced significant decrease of bacilli burdens and high production of pro-inflammatory cytokines, while the opposite was seen when HMGB1 was administered. Blocking HMGB1 activity or administrated it in high amounts during late infection worsening the disease.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52536
Hernández Pando, Rogelio; Barrios Payán, Jorge; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Hernández Ramírez, Diego; et al.; The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis; Public Library of Science; Plos One; 10; 7; 7-2015; 1-14; e0133200
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52536
identifier_str_mv Hernández Pando, Rogelio; Barrios Payán, Jorge; Mata Espinosa, Dulce; Marquina Castillo, Brenda; Hernández Ramírez, Diego; et al.; The role of high mobility group box 1 protein (HMGB1) in the immunopathology of experimental pulmonary tuberculosis; Public Library of Science; Plos One; 10; 7; 7-2015; 1-14; e0133200
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0133200
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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